Fracture distribution controls fluids circulation in fault damage zones, with evident implications for fault mechanics, hydrogeology and hydrocarbon exploration. Being usually characterized by a strong damage and structural complexity, this is of particularly importance for relay zones. We investigated the fracture distribution within a portion of a relay ramp damage zone pertaining to the Tre Monti fault (Central Italy). The damage zone is hosted within peritidal carbonates and located at the footwall of the relay ramp front segment. We analysed the distribution of the fracture density in the outcrop adopting a multidisciplinary study, involving classical and modern structural geology techniques: (1) scanlines measured in the field, (2) oriented rock samples, and (3) scan-areas performed on a virtual outcrop model obtained by aerial structure from motion. Scanlines and virtual scan-areas show that fracture density increases with the distance from the front segment of the relay ramp. Moreover, all the methods highlight that supratidal and intertidal carbonate facies exhibit higher fracture density than subtidal limestones. This trend of fracture density has two main explanations. (1) The damage is associated with the relay ramp development. Approaching the centre of the relay ramp (i.e., moving away from the front segment) an increase in the number of subsidiary faults with their associated damage zones produces high fracture densities. (2) The increase in fracture density can be attributed to the increasing content in supratidal and intertidal carbonate facies that are more abundant in the centre of the relay ramp. Our results highlight structural and lithological control on fracture distribution within relay ramps hosted in shallow water limestones.