From May to June 2011, during a survey of the wheat-growing areas in Meknes in the Saïs Region of Morocco, several cyst nematode populations were detected. Sampling was performed 1 month before wheat (Triticum durum) harvest, in fields showing patches of stunted plants. Plants were growing poorly, had chlorotic lower leaves, and a reduced numbers of ears. Root systems were short and had a bushy appearance because of increased secondary root production. No cysts were visible on the roots, but were found in the soil. Cysts were collected from soil on 200-µm sieves by the modified Cobb decanting and sieving method (1) and identified by morphology and internal transcribed spacer (ITS)-rDNA sequencing. All isolates were identified as Heterodera avenae except the isolate from Aïn Jemâa. From the latter, key morphological features from cysts and second-stage juveniles (J2) were determined. The cysts (n = 10) had the following characteristics: bifenestrate vulval cone, body length without neck 590 µm (551 to 632 µm), body width 393 µm (310 to 490 µm), neck length 75 µm (65 to 90 µm), fenestra length 64 µm (60 to 72 µm) and width 21 µm (18 to 25 µm), underbridge length 96 µm (85 to 115 µm), vulval slit length 8 µm (7 to 9 µm), vulva bridge width 27 µm (24 to 33 µm), and bullae absent. The J2s (n = 10) had the following characteristics: body length 445 µm (412 to 472 µm), body width 19 µm (19 to 21 µm), stylet length 24 µm (23 to 25 µm), four lateral lines, tail length 50 µm (46 to 54 µm), and hyaline terminal tail 28 µm (24 to 31 µm). Values of the morphological characters were within the range of H. latipons reported by Handoo (3). The bifenestrate cysts with a strong underbridge and no bullae and J2 with a tail length greater than 40 µm, a stylet longer than 15 µm, and four incisures in the lateral field were typical for H. latipons. To confirm the identification, molecular observations were made. DNA was extracted from three juveniles from three different cysts separately (4). The ITS-rDNA region was amplified using the primers 5′-CGT AAC AAG GTA GCT GTA G-3′ and 5′-TCC TCC GCT AAA TGA TAT G-3′ as described by Ferris et al. (2). This resulted in a 1,040-bp DNA fragment. The PCR-products were purified and sequenced (Macrogen, Inc., Seoul, Korea). All sequences obtained (GenBank Accession Nos. per cyst: JQ319035, JQ319036, and JQ319037) were compared with sequences available from the GenBank database (www.ncbi.nlm.nih.gov), including several species of Heterodera. This comparison revealed a sequence similarity of 97 to 99% with H. latipons and 89% or lower with any other species of Heterodera. Morphological and molecular identification demonstrated that the population of cyst nematodes from a wheat field in Aïn Jemâa, Morocco was H. latipons. In the patches with poor growing plants, 65 cysts per 100 cm 3 soil were found. To our knowledge, this detection represents a new record of H. latipons. Since the nematode can cause considerable damage to wheat, one of the main cereals produced in Morocco, care should be taken to prevent the spread to other regions.