INTRODUCTION: Human Papilloma Virus (HPV) is a sexually transmitted virus that leads to condyloma, genital warts. Public health awareness of HPV infection, ways of protection and information about immunization are provided by the health staff. In this study, we aimed to evaluate the level of knowledge of midwives and nurses working at the hospital about sexually transmitted and highly prevalent ... [Show full abstract] HPV virus.MATERIAL AND METHOD: The questionnaire in the study was applied to midwives and nurses working at Health Science University, Bursa Yüksek İhtisas Training and Research Hospital. The questionnaire consists of total 21 questions, including 6 questions about demographic characteristics and 15 questions about HPV infection and vaccinations.RESULTS: A total of 163 midwives and nurses completed the questionnaire. Four questionnaires were excluded because all the questions were not answered. Fourteen (8.6 %) participants stated that they did not hear about HPV infection. 88.3% of them knew that HPV infection could cause cancer.CONCLUSION: The studies that primarily aim to increase the awareness of midwives and nurses about HPV infection, may lead to reduce genital warts and cervical cancer. It will play an important role in increasing the awareness of the community about HPV infection and cervical cancer by reviewing curriculums in midwifery and nursing schools and keeping up-to-date information in training courses for actively working health staff.