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Susceptibility of different materials and antifouling coating to macro-fouling organisms in a high wave-energy environment



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The Journal of Ocean Technology, Vol. 15, No. 1, 2020
Copyright Journal of Ocean Technology 2020
Navarrete, Parragué, Osiadacz, Rojas, Bonicelli,
Fernández, Arboleda-Baena, Finke, and Baldanzi
boost our knowledge of biofouling risks in high
wave-energy habitats.
Who should read this paper?
This paper is of interest to experts in the fields of marine renewable energy,
aquaculture, and marine ecology as well as the general public.
Why is it important?
In this paper, the authors present results of an experimental study designed
to evaluate the susceptibility and selectivity of commonly used materials
in the maritime industry to colonization and biomass accumulation of
encrusting subtidal species. Specifically, they assess whether biofouling
composition, main species, and biomass accumulation rates are different
among three materials (aluminum, high-density polyethylene, and steel) at
two different depths (5 m and 15 m) in central Chile.
About the authors
Dr. Sergio Navarrete is a Professor at Pontifical Catholic University of
Chile. His expertise lies within the fields of community ecology, trophic
ecology, population ecology, and biogeography. He focuses on the
dynamics and diversity of coastal marine communities and the influence
they have on oceanographic and climatic processes from local to
regional scales.
Mirtala Parragué is a marine biologist with Universidad de Concepción,
Chile, and Laboratory Manager of the Marine Corrosion and Biofouling
Laboratory in the Marine Energy Research and Innovation Center
(MERIC) at Estación Costera de Investigaciones Marinas owned by
Pontificia Universidad Católica de Chile. Her interests are focused on the
biofouling community in the subtidal zone in south and central Chile,
especially in recruitment patterns, colonization strategies, and risks
evaluation of the species that live in this area and how these processes are
influenced by different biotic and abiotic factors.
Nicole Osiadacz has a degree in administration in ecotourism from Andres
Bello University, is a professional diver of the Chilean Army, and a PADI
diver instructor. Her interest is focused on the subtidal marine ecology and
underwater photography. She is working in the Marine Research Coastal
Station since 2016 as a marine research assistant of subtidal field surveys
with Dr. Sergio Navarrete.
Sergio Navarrete
Mirtala Parragué
Nicole Osiadacz
Francisca Rojas
Jessica Bonicelli
Accumulating Biofouling – and Knowledge
The Journal of Ocean Technology, Vol. 15, No. 1, 2020
Copyright Journal of Ocean Technology 2020
Miriam Fernández
Clara Arboleda-Baena
Randy Finke
Simone Baldanzi
Francisca Rojas graduated from the Universidad de Valparaíso as a marine
biologist with interest lying within the fields of marine ecology and
biology. Since 2015, she has worked at the Estación Costera de
Investigaciones Marinas with Dr. Sergio Navarrete. The lab team is
focused on the study of the dynamics and diversity of coastal marine
communities and the influence they have on oceanographic and climatic
processes from local to regional scales. She manages the field surveys in
the intertidal and laboratory logistics.
Jessica Bonicelli is a biological oceanography researcher at the Instituto de
Fomento Pesquero, Chile. Her interests are in numerical simulation,
numerical modelling, and data analysis.
Dr. Miriam Fernández is a Professor at the Pontificial Catholic University
of Chile. Her research focuses on the life history traits of marine
invertebrates and their ecology for the management and conservation of
marine resources.
Clara Arboleda-Baena is a microbial ecologist who specializes in
interactions between micro and macroorganism in aquatic ecosystems. Her
research is interdisciplinary, occurring in the interface between microbial
molecular ecology, microscopy, and field work.
Randy Finke is a Research Support Specialist at the Pontificia Universidad
Católica de Chile’s Estación Costera de Investigaciones Marinas (ECIM)
in Las Cruces, Chile. He is currently responsible for scuba diving, boating,
oceanographic operations and equipment maintenance, management of
ECIM’s environmental (SST, meteorological, tidal) database, and assisting
visiting researchers with the logistics of their research.
Dr. Simone Baldanzi is an Associate Professor at the Faculty of Marine
Science and Natural Resources at the University of Valparaiso, Chile. His
research focuses on experimental marine ecology, ecophysiology, marine
larval ecology, and evolutionary ecology of marine invertebrates.
The Journal of Ocean Technology, Vol. 15, No. 1, 2020
The Journal of Ocean Technology, Vol. 15, No. 1, 2020
Copyright Journal of Ocean Technology 2020
Sergio A. Navarrete
, Mirtala Parragué
, Nicole Osiadacz
, Francisca Rojas
, Jessica
, Miriam Fernández
, Clara Arboleda-Baena
, Randy Finke
, and Simone
Estación Costera de Investigaciones Marinas, Las Cruces, Facultad de Ciencias Biológicas, Pontificia
Universidad Católica de Chile, Santiago, Chile
Marine Energy Research and Innovation Center (MERIC), Santiago, Chile
Center of Applied Ecology and Sustainability (CAPES), Pontificia Universidad Católica de Chile,
Santiago, Chile
Departamento de Oceanografía y Medio Ambiente, Instituto de Fomento Pesquero (IFOP), Valparaíso,
Facultad de Ciencias del Mar y Recursos Naturales, Universidad de Valparaíso, Viña del mar, Chile
Our knowledge about the interaction of materials used in aquaculture with biofouling species
is largely restricted to sheltered coastal areas. Little is known about the susceptibility and
specificity of different materials, or the effectiveness of antifouling (AF) coatings, to the
incrustation by large biofouling species in high wave-energy environments. Since these
energetic habitats are becoming increasingly targeted by the aquaculture industry, and since
there is increasing concerns about the use of harmful antifouling coatings, it is urgent to
boost our knowledge about biofouling risks in these environments. Here we assessed whether
biofouling composition, main species, and biomass accumulation rates were different among
three materials, aluminum, high-density polyethylene (HDPE), and steel A36, and at two
different depths of exposed shore in central Chile. We hypothesized that either colonization
was material-specific and/or the adhesion of macrofoulers to the different materials (tenacity)
was sufficiently different that waves could remove them from some surfaces more than others.
Additionally, we evaluated the performance of an antifouling paint widely used in aquaculture
operations in Chile. All materials were colonized by macrofouling within three months of
exposure, with no significant differences in either species composition, total cover, or the rate
of biomass accumulation. No significant settlement of macrofouling was found on plates coated
with the antifouling paint after seven months of exposure. The fast growth rates and similar
composition of macrofouling suggest that the large differences in roughness and hydrophobic
character among materials are not sufficient to produce differential settlement or dislodgement
in these biofouling communities. The efficacy of the tested antifouling paint suggest that this
paint could be used as reference when testing more environmentally-friendly coatings, such as
those using biomimetic approaches.
The Journal of Ocean Technology, Vol. 15, No. 1, 2020
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High wave energy regions of the coastal
oceans are receiving increasing attention
as a source of renewable energy and
as areas where many human activities,
including aquaculture, can expand to reduce
environmental externalities and ameliorate
conflicts with other uses, which currently
concentrate in protected habitats [Gentry et
al., 2017A]. The expansion of aquaculture,
the fastest growing food production sector
in the world, from easily polluted protected
bays and fjords to wave energetic open waters
is imminent according to most industry
projections [Bostock et al., 2010; Gentry et
al., 2017A; Gentry et al., 2017B; Troell et al.,
2009]. At the same time, there is increasing
attention to the great potential for clean marine
renewable energy (MRE) extracted from
waves around the world [Arinaga and Cheung,
2012; Cornett, 2008] and a wide diversity of
technologies are being developed and tested to
harvest this energy [López et al., 2013]. In all
these industrial developments, the materials
employed must not only be able to accomplish
and support the intended work, but must also
cope with the challenges imposed by the
inevitable biofouling of marine organisms
[Lacoste and Gaertner-Mazouni, 2015; Titah-
Benbouzid and Benbouzid, 2017]. Indeed,
the mechanical, buoyancy, and hydrodynamic
features of these human-made infrastructures
interact with different biofouling species in
a manner that can make the entire enterprise
economically or mechanically unfeasible
[Lacoste and Gaertner-Mazouni, 2015; Nall et
al., 2017]. Vast regions of the world’s shores
are highly exposed to waves, including the
coasts of Peru and Chile in the southeastern
Pacific [Lucero et al., 2017; Mork et al.,
2010], but yet, because of the logistical and
safety-related difficulties of conducting
detailed observations in wave exposed
habitats, the characterization of biofouling in
these regions is incomplete, at best. To what
extent biofouling colonization and biomass
accumulation vary among different materials
and what is the effectiveness of commonly
used antifouling paints when subjected to
impounding waves has not been evaluated.
The risks of biofouling to human-made
infrastructures result from the interaction
among three factors. First, the set of attributes
of the biota found within the biogeographic
region where the device is deployed, especially
macrofouling organisms (e.g., domination
by macroalgae, flexible hydrozoans, rigid
barnacles, or perforating organisms), which
determine, for instance, the load, lift, and
drag forces exerted on the infrastructure
[Richmond and Seed 1991; Macleod et al.,
2016]. Second, the properties of the material,
such as surface chemical composition,
compliance, roughness, and wettability,
which can alter the colonization by different
fouling species as well as their tenacity
(adhesion force) once established [Schultz,
2007; Titah-Benbouzid and Benbouzid, 2015].
Environmental impact; Engineering; Mathematical models; Hydroacoustic equipment; High
frequency sound sources; Sonar
The Journal of Ocean Technology, Vol. 15, No. 1, 2020
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Third, the local environmental conditions,
including productivity, temperature, flows, and
hydrodynamic forces that control growth rates
of different fouling species. Thus, information
on materials’ performances in one region of
the world and a given type of habitat cannot
be used to inform decisions on another part
of the world and under widely different
environmental conditions [Want et al., 2017].
Here we present results of a field experiment
designed to assess differential susceptibility to
macrofouling organisms by different materials
commonly used in the seagoing industry, in
a highly wave-exposed subtidal habitat of
central Chile.
Many studies have shown that biofouling
community composition varies profoundly
with time of exposure of the surfaces to the
marine environment; from the biofilm that
settles on the surface within hours of exposure
(microfouling), to the large sessile invertebrates
and algae (macrofouling) that dominate the
surface after few to several months [Callow
and Callow, 2011; Dafforn et al., 2011].
Comparisons of material performance and
antifouling methods must therefore be done by
exposing materials for similar periods of time.
Experimental studies that have done so have
typically shown that early succession stages
vary substantially among different materials,
apparently because the physical-chemical
characteristics of the material surface can affect
the colonization process [Carr and Hixon,
1997; Russ, 1977; Salta et al., 2013; Walters
and Wethey, 1996]. However, such differences
among artificial materials generally tend to be
reduced or disappear towards later successional
stages [Anderson and Underwood, 1994;
Chapman and Clynick, 2006]. In some cases,
however, initial differences in colonization can
lead to persistent differences in composition
of the sessile biofouling community of later
successional stages, which can resist new
invasions [Petraitis and Dudgeon, 2005;
Sutherland, 1974; Vieira et al., 2018]. To
what extent differences in initial stages of
succession, which are commonly observed
in different material surfaces, can determine
longer-term or final community composition
depends on the attributes and diversity
of the species that form part of the local
biofouling community that settle and engage
in competition for space [Navarrete, 2007;
Paine, 1984]. These interactions and species
attributes will determine whether succession is
highly “canalized” towards a common mono-
specific state, regardless of initial differences,
or if alternative states are feasible [Berlow,
1997; Caro et al., 2010; Petraitis and Dudgeon,
1999; Wootton, 2005]. From the point of
view of aquaculture and MRE applications,
different states of the biofouling community can
represent widely different risks for the operation
of the technology and supporting infrastructure,
including maintenance schedules.
Besides differences in initial fouler
colonization among materials, often associated
to differences in surface hydrophobic
properties or wettability, the adhesion strength
of different sessile species to the surface
(tenacity) varies among the different materials,
primarily due to differences in surface
roughness [Howell and Beherends, 2006].
Such differences in tenacity may not amount
to large changes in species composition in
calm waters, but may produce differential
dislodgement of individuals on different
materials in wave-exposed or high fluid
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speed environments
[e.g. Schultz, 2007].
Indeed, dislodgment of
large sessile organisms
is common in wave
exposed shores [Bertness
et al., 1998; Carrington et
al., 2009] and it may lead
to different biofouling
communities on different
substrates. Thus, we
hypothesize that the
different materials
commonly used in the
aquaculture and MRE
industries can sustain different biofouling
communities, with different cover and
biomass accumulation rates, which represent
widely different risks to the equipment and
infrastructure operation and maintenance.
Here, we experimentally assess the
susceptibility to biofouling of three different
materials commonly used in the aquaculture
and shipping industries, which are also
part of the emerging MRE technologies.
Since previous studies reported differences
in rates of biomass accumulation between
depths [Navarrete et al., 2019], we tested the
materials and paint at two depths, above and
below the thermocline, for a period of nearly
eight months.
The study was conducted in the Bay of
Cartagena, a section of the coast of central
Chile directly exposed to prevailing southern
waves (Figure 1A). The site represents
an upwelling shadow [sensu Graham and
Largier, 1997], where waters remain slightly
warmer (1-2°C), nutrients slightly lower, and
phytoplankton blooms more common than at
sites to the north and south [Narváez et al.,
2004; Tapia et al., 2009; Wieters et al., 2003].
General hydrographic characteristics of the
study site have been previously described
[Bonicelli et al., 2014A; Bonicelli et al.,
2014B; Kaplan et al., 2003; Lagos et al., 2005;
Narváez et al., 2004; Navarrete et al., 2015;
Piñones et al., 2005]. The name of the study
site is somewhat deceiving because Cartagena
Bay is not a protected bay, but it is fully
exposed to the predominant incoming waves
propagating from the south (see [Parra & Beyá,
2017],, and
“Results” in this paper).
We deployed four moorings at 20-21 m
bottom depths across the Bay of Cartagena,
covering about 5 km and separated by about
1.5 km (Figure 1A). In this manner, and since
the hydrographic conditions vary between
the northern and southern ends of Cartagena
Bay [Bonicelli et al., 2014A], we expected
Figure 1: A) Map of study site at the open embayment of Cartagena showing position of the moorings
and bathymetry. B) Monthly mean seawater temperature (+ SE) at surface (5 m deep) and deep (15
m deep) depths. C) Maximum daily wave height from the Global Forecast System numerical model
used by Windguru (see text). The map of study site has been adjusted from Navarrete et al. [2019].
The Journal of Ocean Technology, Vol. 15, No. 1, 2020
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to capture sufficient spatial variation in
biofouling across the moorings. Each mooring
consisted of a 150 kg weight and a line of high
tenacity polypropylene rope with a subsurface
buoy at about 1 m deep, and two small (50
cm diameter) buoys along the rope to keep
the line tense even during passing waves.
Testing frames were made of 2.5 cm diameter
hydraulic-rated PVC, forming a 70 x 40 cm
rectangle, and were deployed at two depths in
each mooring line, at about 5 m deep, above
the seasonal thermocline [Bonicelli et al.,
2014A; Narváez et al., 2004], and at about 15
m deep, below the thermocline. One replicate
of a 10 x 10 cm biofouling plate made of each
of the three materials and antifouling paint
(four treatments) was affixed to each frame
in a completely random pattern at each depth.
The size and hydraulic behaviour of the frame
did not allow us to include replicate plates of
each treatment within a frame. Additionally, we
attached temperature loggers (Onset Tidbit®),
which recorded temperature every 10 minutes
at about 2 m depth above the first frame and
at about 19 m depth below the deeper frame
in each mooring line. Wave height data were
obtained from Windguru (www.windguru.
cz), which is based on Global Forecast System
(GFS) numerical model. Predicted wave height
from this model has been shown to correlate
well with in-situ recorded waves at the study
site [Navarrete et al., 2015].
Placing the experimental plates above and
below the seasonal thermocline allowed us
to maximize differences in environmental
conditions within the water column. Besides
the seasonal differences in sea surface
temperature, one of the major drivers of
metabolic activity in ectothermic animals, the
thermocline typically marks abrupt changes in
concentration of Chlorophyll-a (higher right
above or at the thermocline) [Narváez et al.,
2004], the relative abundance of competent
larval stages [Bonicelli et al., 2014A; Vargas
et al., 2006], the influence of river plumes
[Piñones et al., 2005; Vargas et al., 2006], or
the frequency of hypoxic events.
Materials tested were a) aluminum
AA1100 temper h14 (99.0% aluminium,
with aluminium oxide finish), which is
used in many applications because of its
high corrosion resistance, b) High Density
Polyethylene (HDPE), a positively buoyant,
high durability material used in most
aquaculture operations, and c) construction
steel A36, used in the shipping industry and
in the experimental MRE technologies. Since
anticorrosive and antifouling paints do not
adhere well to aluminum and HDPE, they are
typically used without coatings. In addition
to these materials, we tested the efficacy of a
widely used antifouling, copper-based paint
(Ocean Jet, commercially
available in Chile. This antifouling paint is
based on high solid epoxy polyamine (with
micaceous iron oxide). Steel plates (A36)
with antifouling were painted at the ACN
Ltda. in Valdivia, Chile, following the same
procedures used in ships.
We measured surface roughness (SR) and
hydrophobic index (H) for each of the
three materials tested (aluminium AA1100,
HDPE, and steel A36) and for the AF
coating. We haphazardly chose one plate of
each surface and measured SR and H along
two perpendicular transects within a 4 x 2
cm area. Because of the widely different
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roughness levels of the different plates, two
different instruments were used to measure
SR. The SR of aluminum and HDPE were
measured using a Bruker Innova Atomic
Force Microscope (AFM) and the SR of
steel and AF coating were measured using a
BioLogic Optical Surface Profiler (OSP470).
All measurements were then expressed
as RMS (mean square root) and given in
. The surface hydrophobic index was
measured by determining the water contact
angle (WCA) following ASTM D7334-08
(ASTM International, 2013), using water-type
II reagent (distilled) after ASTM D1193-
06 (ASTM International, 2011). The index
was calculated by employing critical surface
tension theory, assigning hydrophilic index
WCA < 45°, hydrophobic index to WCA >
90°, and intermediate index for 45° < WCA <
90°. Finally, the quality of polymer adhesion
was verified by means of a tape-and-peel test,
conducted according to ASTM D3359-09ε2
standard (method A, ASTM International,
2010). Before deployment and during the
second and third monitoring dates (see
below), each plate was weighed in a precision
balance, both in air and submerged in water,
and then individually marked. Monitoring
was conducted by scuba diving. Plates were
photographed in-situ, then removed from the
frame and taken aboard the ILAN research boat
of the Estación Costera de Investigaciones
Marinas (ECIM), where they were weighed
on a precision scale after carefully cleaning
the sides and back, photographed, and then the
cover of all species was recorded with a 10 x
10 cm quadrat with 100 intersection points.
All species observed on the plate surfaces,
without disturbing the settled organisms,
were registered. Plates were then redeployed
in their original positions. Biofouling plates
were initially deployed in July 2017 and
monitored in November 2017 (97 days’
exposure) and then again in March 2018 (219
days’ exposure). On the second monitoring
date, several frames had been lost due to large
waves, which impeded us to analyze time
trajectories. Loss of moorings due to fatigue of
the anchoring lines did not allow us to extend
monitoring beyond March 2018.
Data Analyses
Total biofouling biomass on each plate was
obtained by subtracting the weight of the plate
from the total mass recorded during monitoring.
Total biofouling biomass accumulation rates
were calculated as the weight of biomass (g)
accumulated per plate (100 cm
) divided by the
number of days the plates were exposed in the
field (g/day/100 cm
Statistical comparisons of materials and depths
were conducted for biomass accumulation
rates and total cover and were restricted to the
first monitoring, after 97 days of exposure,
because two of the four replicates were lost
on the second monitoring. The experimental
design corresponded to a randomized complete
block design (four moorings as blocks),
with a two-way orthogonal structure within
each block, which corresponded to materials
(fixed factor with four levels) and depth (fix
factor with two levels). Biomass rates were
log-transformed, and cover data were arcsin-
square root transformed before analysis to meet
homoscedasticity assumptions and improve
normality. Results showed that blocks did
not make a significant contribution to overall
variance, even after using a conservative
significance level alpha =0.1 to reduce
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type II error rate. Therefore, blocks and the
interactions with other factors were pooled
with residual variance (see “Results”). Because
no species settled and grew on the plates with
antifouling paint (see “Results”), this material
was not included in statistical analyses.
To examine possible change in species
composition among materials, we conducted a
non-metric multidimensional scaling (nMDS)
ordination using presence/absence data
including all sessile species recorded on the
plates. A Bray-Curtis similarity index was used.
As shown in previous studies, seawater
temperature was about 2-3 degrees warmer
and more seasonal at 5 m deep above the
thermocline than at 15 m deep below the
thermocline (Figure 1B). The study included
the austral winter period, when the water
column is well mixed and surface and bottom
temperatures are similar. Wave height exceeded
3 m during most of the study, with large storm
waves of over 5 m height in winter months and
a clear decrease towards spring and summer
months (Figure 1C).
The most common components of the
macrofouling were hydrozoans (primarily
Obelia geniculata), barnacles (primarily
Notobalanus flosculus and Austromegabalus
psittacus), mussels, and the tunicate Pyura
chilensis, which reached about 1% cover over
the duration of the study (averaging both
monitoring dates and depths, Table 1). The
encrusting alga Hildenbrandia lecannellieri
reached 30% in one plate of aluminum material
after three months’ exposure, but it was not
present on any other plate of the same or
different material. By the second monitoring
(> seven months), Hildenbrandia lecannellieri
had been completely overgrown by Obelia.
Other species observed on the plate surface
never reached over 1.5% cover.
At both depths, all materials, except plates
with antifouling paint, were rapidly colonized
by biofouling, which covered over 90% of
Table 1: List of encrusting taxa encountered throughout the study period at the two depths (5 and 15 m) and any of the three materials and
exposure times. Mean cover is the long-term average across depths and monitoring times.
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the plate surface within the three-month time
elapsed between deployment and the first
monitoring (Figures 2-3; Figure S1). Over
this time, no differences between the surface
(5 m) and deeper (15 m) plates were observed
(Figure 3, Table 2). Although total biofouling
cover was slightly lower on aluminum than the
other two materials (91% versus 95%), these
differences were not statistically significant
at any depth (Table 2). The notable exception
was the steel plate painted with antifouling
paint, where virtually no macrofouling
settled throughout the seven months of the
experiment. This treatment was therefore left
out of statistical comparisons. After three
months of exposure, steel plates showed clear
signs of surface corrosion and some bacterial
mats were apparent among hydrozoans. By the
time of the second monitoring, the surface of
these plates had laminated and, in some cases,
peeled off the plate, removing in this way the
fouling organisms (Figure 2).
Rates of biomass accumulation (g/day) in the
100 cm
plates varied somewhat among the
three materials (Figure 4), but the differences
were not consistent between depths, and they
were not statistically significant (Table 2).
Overall, similar biomass accumulation rates
were observed in shallow and deeper waters
over the three-month time exposure. Again, no
settlement of macrofoulers was observed on
plates with antifouling paint.
By far the most abundant species in terms of
cover was the hydrozoan O. geniculata, which
covered over 80% of the plate surface after
three months of exposure at 5 m deep and over
70% at 15 m deep in all three materials (Figure
5A, B, C). The large barnacle A. psittacus also
settled in all materials but did not surpass 5%
cover within the first three months of plate
exposure. After seven months, this barnacle
species, as well as the mussel Semimytilus
algosus and the tunicate P. chilensis, were
present in all materials and started to overgrow
O. geniculata and reached over 10% cover
in most plates (Figure 5A, B, C). Biofouling
species composition, i.e., considering only
the presence/absence of species on the
different materials, showed that the biofouling
communities were not different among the
different materials (Figure 5D).
The surface roughness indices were widely
different among the three materials and the AF
coating (Table 3). Particularly, aluminium and
HDPE had similarly low SR values, compared
to steel and the AF paint (Table 3). The water
contact angle of HDPE, steel, and the AF
coating showed similar values of intermediate
hydrophobic character (see Table 3), while
aluminium had WCA exceeding 90°, indicating
high hydrophobic character (Table 3). Regardless
of these physical differences among surface
materials, no correlations were found with
biofouling biomass or cover (see Figure S2).
The adverse effects of biofouling growth
on all human-made structures deployed at
sea is an important consideration for all
marine industries. Strategies to reduce the
problem must also reduce the impacts in the
environment and different materials may
exhibit different susceptibility to colonization
and growth by diverse macrofouling species
and under different conditions. Our results
show that three of the most common materials
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Figure 2: Photographs of
representative plates taken
at the first (about three
months’ field exposure) and
second monitoring (about
seven months’ exposure) for
each material used (HDPE,
aluminium, steel), antifouling
paint (AF paint), and at 5 m of
depth and 15 m of depth.
Figure 3: Mean total cover (+SE)
of biofouling that settled on
the experimental plates of the
different materials and at 5 m of
depth and 15 m of depth.
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Figure 4: Mean rate of biomass
accumulation (g/day/100 cm2)
on the experimental plates of the
different materials observed at 5 m
of depth and 15 m of depth.
Figure 5: Average cover (%) of the main fouling species observed on A) aluminium, B) steel, and C) HDPE, during the first monitoring (about
three months’ field exposure) and at the second monitoring (about seven months’ exposure) and at the two deployment depths (5 and 15
m). D) Multivariate ordination of biofouling community found on plates of the different tested materials at the two depths using non-metric
multidimensional scaling on presence/absence data. Legend with colour codes are reported below the graphs.
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used in the maritime industry are equally
susceptible to fouling and exhibit similar
biomass accumulation rates and biofouling
community composition in wave exposed
habitats of central Chile. Thus, even though
studies on other shores have found that
surface roughness and hydrophobic index can
have large effects on biofouling composition
and colonization rates [e.g., Calow and Calow,
2011], we found no evidence of such effects
on macrofoulers in our study, highlighting the
strong dependency of macrofouler-surface
interactions on environmental conditions
[Howell and Beherends, 2006]. We also
found that a copper-based antifouling paint
prevents any colonization by macrofoulers
for over seven months under these high-
energy environmental conditions. We suggest
that this paint could be used a reference
when testing more environmentally-friendly
coatings, such as those using biomimetic
approaches [Salta et al., 2010]. We discuss
these findings in light of results obtained in
other wave-exposed shores of the world and
from protected conditions in Chile.
Several studies have shown that the biofouling
community composition varies among different
substrates at the early succession stages because
the physical-chemical characteristics of the
material surface can affect the colonization
process [Carr and Hixon, 1997; Russ, 1977;
Walters and Wethey, 1996]. Anderson and
Underwood [1994] compared different
substrates (concrete, plywood, fibreglass,
and aluminum) in one bay of Australia,
and concluded that the assemblages were
significantly different after one or two months
Table 2: Results of two-way ANOVA (analysis of variance) comparing a) total biofouling cover (% arsin-sqrt-transformed) and b) rates of
biofouling biomass accumulation (g/day, log-transformed) in the 100 cm2 plates among the three materials on which we observed settlement
(aluminum, HDPE, steel) and the two deployment depths (5 and 15 m).
Table 3: Results of the analysis of surface roughness and hydrophobic character of the three materials (aluminium, HDPE, and steel) and
antifouling (AF) paint. RMS=Root Mean Square; WCA=Water Contact Angle; High=WCA<90; Intermediate= 45<WCA<90.
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of exposure, but they become similar after four
or five months of submersion, depending on
the season of the year. Thus, after a differential
colonization processes on various surfaces,
the community becomes more similar in all
the materials by converging to similar sets of
dominant species [see also Andersson et al.,
2009; McGuiness, 1989]. Similar results were
observed by Chapman and Clynick [2006],
who compared organisms on different types of
waste material (tires, wood, metal, and natural
sandstone) in Australian estuaries and found,
after 19 months, that algal and invertebrate
compositions were similar among the different
substrates. In a different study conducted
along two breakwaters and a natural reef on
the shore of Dubai, United Arab Emirates,
Burt et al. [2009] compared various materials
(concrete, gabbro, granite, and sandstone) and
concluded that, after one year, observed site-
specific differences in recruitment were more
important in determining benthic community
structure and coral recruitment than differences
among substrate materials. Similar results
have been observed along the Mediterranean
shore of Italy [Chapman and Bulleri, 2003].
Thus, most studies that have experimentally
exposed different surfaces to the same or
similar environmental conditions and, in this
manner, they have controlled the time of
biofouling succession, indicate that the type of
substratum can affect initial colonization but
does not usually alter later successional stages
and the final established biofouling community
is generally similar across materials. Similar
results were observed in our experimental
study in wave-exposed subtidal habitats, as
well as in a previous study in which acrylic and
ceramic tiles were compared at the same site
[Navarrete et al., 2019]. Our results show that
after only three months, HDPE, steel A36, and
aluminum were almost completely covered by
biofouling and exhibited the same dominant
species, the hydrozoan Obelia geniculata, and
similar species composition. After seven months
of exposure, competitively dominant species,
especially large-bodied barnacles and tunicates,
started to overcome O. geniculata in all
surfaces, but Obelia was still the most abundant
species in terms of surface cover. Thus, the risks
of fouling among these materials for aquaculture
and MRE applications are the same. It should be
noted, however, that the loss of metal material
(lamination and detachment of surface layers)
in the untreated (unpainted) steel plates after
three months also removed some of the attached
organisms, which would lead to differences in
the established community with other materials.
But loss of structural material can hardly be a
useful antifouling strategy, and we consider that
during the useful period of time untreated steel
can be deployed (< three months), the fouling
risk is similar to the other materials.
As other authors have emphasized, caution
must be exerted when comparing biofouling
communities in different surfaces through
surveys of existing structures in different
habitats and for sometimes unknown periods
of time [Connell and Glasby, 1999; Macleod
et al., 2016; Manríquez et al., 2014; Want et
al., 2017]. For instance, Connell and Glasby
[1999] compared various surfaces (rocky reef,
sandstone (brick) retaining walls, fibreglass,
concrete pontoons, concrete pilings, and wooden
pilings with bark and stripped of bark) with
different times of submersion in an Australian
shore and found differences of compositions
among the surfaces. But such differences
could be best explained by differences in
The Journal of Ocean Technology, Vol. 15, No. 1, 2020
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environmental conditions, orientation, and wave
exposure, instead of surface materials, as also
shown in other studies [Bulleri and Chapman,
2004]. Because of slight or large differences
in environmental conditions, total biofouling
species diversity is typically higher in studies
conducting surveys across space than in studies
that expose materials to more homogeneous
conditions. Such differences in species richness
may also confuse conclusions regarding true
biofouling susceptibility across materials and
emphasize the importance of testing antifouling
strategies in a replicated test bench where
environmental conditions are similar and time of
succession can be controlled.
Our results also show the very high rates of
biomass accumulation attained in southeastern
Pacific coastal waters [Navarrete et al., 2019;
Viviani and DiSalvo, 1980]. After seven months,
we observed the equivalent of over 30 kg/m
biofouling biomass. At the end of this period of
time, most of this biomass (> 65%) was due to
large rigid barnacle A. psittacus and the large
tunicate P. chilensis, which, besides the large
weight they impose on the structure, can greatly
increase drag forces in moving objects in the
water, such as boats or turbines. Unfortunately,
loss of replicate mooring lines prevented
us from running more detailed statistical
comparisons. It is interesting to note that in this
study we did not observe differences in biomass
accumulation rates between depths, above (5
m deep) or below (15 m deep) the thermocline.
This result contrasts with a previous study
at the same site showing significantly larger
biomass accumulation on acrylic and ceramic
tiles in water shallower than the thermocline
than below [Navarrete et al., 2019]. We believe
the difference between studies is at least partly
related to the fact that water temperature was
particularly low during most of the spring 2017.
Mean temperatures during austral spring and
summer were between 1 and 1.2 degrees colder
in 2017 than over the equivalent period in 2016,
and more homogeneous between 5 and 15 m
deep. In 2016 surface water remained below
12.5°C until mid-December, while it had already
reached over 13.5° in early November 2017.
Such low temperatures in surface waters into
late spring may have reduced the reported depth-
stratification in biofouling biomass accumulation
in 2016 as compared to previous years.
Application of effective antifouling strategies
is therefore absolutely necessary for most
aquaculture applications [Bostock et al.,
2010] and to test most of the wave-energy
technologies currently being developed
[López et al., 2013; Want et al., 2017]. To
have a reference to compare against the
untreated surface materials, here we tested
the effectivity of a commonly used copper-
based antifouling paint, Ocean Jet, applied
on steel plates following the same procedure
used on ships hulls. This initial comparison
showed that over the >seven-month long
exposure, the antifouling paint was effective
in preventing settlement by all early and
late successional macrofouling species,
including macroalgae, sessile, and mobile
invertebrates. Invertebrates and macroalgae
were observed on the bolt head that held the
plate in place and on the PVC frame right next
to the plate, suggesting that the antifouling
action is primarily by direct contact with
settling organisms. However, copper and
zinc-based coatings leach these elements
into the environment, where over time they
can accumulate and have large negative
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environmental impacts [Dafforn et al., 2011;
Soroldoni et al., 2017; Turner, 2010]. Yet,
over the short term, some studies show no
negative effect of exposure to antifouling
copper paints on key intertidal grazers [Corte
et al., 2017]. It is therefore urgent to increase
research on alternative antifouling products
and strategies that are naturally found in the
environment where human-made structures
are deployed [Silva-Aciares and Riquelme,
2008; Almeida and Vasconcelos, 2015; Salta
et al., 2010] and to evaluate the effect of
these artificial habitats on macroinvertebrates
colonization [Fernandez-Gonzalez et al.,
2016]. The efficiency of the tested antifouling
paint and apparent harmless local effects in
nearby surfaces suggest this paint can be used
as a reference to contrast environmentally-
friendly antifouling strategies in wave-
exposed environments.
In summary, different artificial materials
commonly used in the maritime industry
were rapidly colonized and overgrown
by encrusting biofouling species of wave
exposed shores of central Chile. The
composition, cover, and the rate of biomass
accumulation of the main fouling species
were similar among the three materials tested,
and similar at two different depths, above
and below the thermocline. The hydrozoan
Obelia geniculata was the most frequent and
abundant (cover) species in all surfaces, and
the larger bodied barnacle Austromegabalus
psittacus, mussels, and tunicates increased
in abundance later in succession, accounting
for most of the wet biomass. We also
showed that differences in surface roughness
and hydrophobic properties among tested
materials were not sufficient to affect the
rapid growth rate of the most common
biofouling species found in the species pool
at our study site. Finally, an antifouling
paint widely used in shipping operations
was very effective in preventing all fouling,
macroalgae, and invertebrates at both depths
and through more than seven months.
The authors thank Professor J. Francisco Armijo
(Faculty of Chemistry and Pharmacy, Pontifical
Catholic University of Chile) for providing the
analysis of surface roughness and hydrophobic
character for the materials tested in this study.
This study was possible thanks to financial
support of the Marine Energy Research and
Innovation Center, MERIC, an applied research
centre funded by the Ministry of Energy and the
Production Development Corporation, project
CORFO 14CEI2-28228. Additional funding was
provided by FONDECYT, grant N°3160294
to Jessica Bonicelli, grant #1151095 and grant
#1160289 to Sergio A. Navarrete.
Sergio A. Navarrete , Mirtala Parragué , Nicole
Osiadacz, and Randy Finke conceived the
experiment, the experimental design, and the
mooring test frame system. Mirtala Parragué,
Nicole Osiadacz, Francisca Rojas, Jessica
Bonicelli, and Randy Finke participated in
the field work and/or laboratory analyses.
Sergio A. Navarrete, Mirtala Parragué, Nicole
Osiadacz, Francisca Rojas, Jessica Bonicelli,
Miriam Fernández, Clara Arboleda-Baena,
Randy Finke, and Simone Baldanzi discussed
the results, analyzed data, and contributed to
manuscript preparation.
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Figure S1: Total cover of
biofouling by monitoring date
after 97 and 219 days.
Figure S2: Scatter plots between
biofouling measures and the physical
properties of the materials used (HDPE,
aluminum, steel, and antifouling paint).
A) Total biofouling biomass and surface
roughness; B) Total biofouling biomass
and hydrophobic index; C) Total cover
and surface roughness; D) Total cover
and hydrophobic index.
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... Most knowledge about biofouling and most experimental tests of AF methods have been conducted in the laboratory or in wave-protected habitats, usually within bays and port harbors. The requirements of the MRE, oceanic aquaculture, desalination, and other industries are rapidly expanding this knowledge to high-energy environments [32][33][34][35]. We conducted a replicated field experiment over more than one year in a highly wave-exposed environment to test biofouling colonization and accumulation rates on three different materials typically used in the shipping, aquaculture, and desalination industries. ...
... The general hydrographic characteristics of the study site-including broad patterns of circulation related to wind forcing, phytoplankton, wave regimes, and riverine inputs-have been previously described [36,[39][40][41][42][43][44]. In addition, the encrusting community of wave-exposed environments and successional trends at different depths have been previously described [32,33]. ...
... Most knowledge about biofouling and most experimental tests of AF methods have been conducted in the laboratory or in wave-protected habitats, usually within bays and port harbors. The requirements of the MRE, oceanic aquaculture, desalination, and other industries are rapidly expanding this knowledge to high-energy environments [32][33][34][35]. We conducted a replicated field experiment over more than one year in a highly waveexposed environment to test biofouling colonization and accumulation rates on three different materials typically used in the shipping, aquaculture, and desalination industries. ...
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Biofouling control on human-made structures and seagoing technologies that minimize environmental impacts is a major focus of research in marine industries. However, the most widely used antifouling (AF) method is still copper-based coatings. Some “eco-friendly” approaches are commercially available but have been scarcely tested in natural conditions, especially high-energy environments. We conducted a replicated long-term field experiment in a highly wave-exposed, high productivity coastal environment to test three untreated materials used in maritime industries, two traditional copper-based AF coatings, and two materials offered as “eco-friendly” AF in the market (i.e., a slow-copper release and a self-adhesive, fiber-covered, skin-like coating). We showed that biofouling cover and biomass increased at similar rates over time among all untreated materials, including the skin-like AF. The two traditional copper-based AF coatings and the slow-release AF paint both showed similarly low biofouling biomass and richness, demonstrating their efficacy after 12 months in the field. Although the “eco-friendly” slow-release technologies are not completely innocuous to the environment, we suggest this approach over the more environmentally aggressive traditional copper paints, which are the most widely used in aquaculture and shipping industries today. However, further research is needed to test whether their environmental impact is significantly lower in the long-term than traditional AF paints, and therefore the search for non-toxic coating must continue. The fortuitous settlement and growth of sea urchins in our experiments also suggest that a combination of “eco-friendly” AF and biological control would be possible and should be further investigated. The skin-like coatings must be tested under different environmental conditions, and they are not recommended in wave-exposed coastal habitats.
... The structure and speed of development of micro-and macro-fouling communities are influenced by many environmental variables, such as current flow, light availability, nutrient load (Qian et al., 2007;Dang and Lovell, 2016;de Carvalho, 2018), as well as by biological factors, such as the availability, growth rate and metabolic state of fouling species and inter-species interactions (Dang and Lovell, 2016;Pollet et al., 2018;Zhang et al., 2019). Such combination of environmental and biological factors leads to temporal and spatial variations in the composition of fouling communities Lovell, 2000, 2016;Oberbeckmann et al., 2016;Navarrete et al., 2020). ...
... Owing to the complexity of the biofouling process, and the involvement of many exogenous and endogenous factors, most studies related to temporal succession of biofouling communities have focused on either micro- (Rampadarath et al., 2017;Pollet et al., 2018;Antunes et al., 2020) or macro-fouling (Naser, 2017;Briand et al., 2018;Navarrete et al., 2020) communities, rather than both together. Short-term, i.e., hourly, and daily succession of microfouling communities has been investigated to identify primary and secondary colonizers (Dang and Lovell, 2000;Pollet et al., 2018;Antunes et al., 2020). ...
... Long-term succession studies mostly involved analyzing microfouling communities that were developed for two (Satheesh and Wesley, 2012;Elifantz et al., 2013) or four weeks per season (Antunes et al., 2020), while analyses pertaining to monthly succession were quite limited (Pinto et al., 2019). Conversely, succession of macrofouling communities has been extensively investigated beginning from two until a maximum of 84 months (Macleod et al., 2016;Naser, 2017;Navarrete et al., 2020). The exploration of spatial effects on micro- (Briand et al., 2012;Muthukrishnan et al., 2017;Rampadarath et al., 2017) and macro-fouling (Macleod et al., 2016;Navarrete et al., 2020) community development extended to several ocean basins, such as the Arabian Gulf (AG), and the Indian and Atlantic oceans. ...
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Marine biofouling is a complicated process involving changes within micro- and macro-fouling community, species co-occurrence, and inter-taxa association patterns. An investigation of all above-mentioned aspects has rarely been conducted so far. Our study aimed to compare the monthly succession of the biofouling community developed at two locations each in the north- (Kuwait) and south-west (Oman) of the Arabian Gulf (AG) over 6 months, and to explore the association patterns within microfouling and between micro- and macro-fouling communities on a temporal and spatial scale. Spatio-temporal effects on the abundance and composition of micro- and macro-fouling communities were detected based on total biomass, bacterial and phototroph abundances, macrofouling coverage and 16S rRNA gene sequencing. We documented the development of distinct ecological niches within the fouling community resulting in fundamentally different succession patterns depending on location. Network analysis revealed nine clusters of highly interconnected co-occurring fouling bacterial taxa (M1-M9), with strong association (both positive and negative) to microalgae and macrofoulers in both Kuwait and Oman. Early stages of Kuwait biofilm showed M7 (cyanobacterial OTUs) positively and negatively associated with the majority of diatoms and macroalgae (Cladophoraceae), respectively, unlike the later stages where M5 (composed of Vibrio spp.) was positively associated with polychaetes ( Hydroides elegans ). While the causal relationships behind the observed inter-taxa associations remain unknown, our study provided insights into the underlying dynamics of biofouling processes encountered in the north- and south-west of the AG. Comprehensive future investigations encompassing transcriptomic or metabolomic tools may be required to address the challenge of interpreting such complicated dynamics over time and space in a continuously changing environment.
... Early work by Crisp and Stubbings (1957) used panels suspended at the surface in the tidal channel at the Menai Strait, in order to investigate the influence of current flow on barnacle settlement patterns. A mid-depth biofouling monitoring system has recently been deployed at a wave exposed site in Chile which may, in future, be used to test wave energy devices (Navarrete et al. 2019(Navarrete et al. , 2020. Monitoring seasonal recruitment onto settlement panels of organisms with planktonic larvae may serve as an effective method of identifying important life history stages of problematic fouling species (Sutherland and Karlson 1977;Underwood and Anderson 1994;Marraffini et al. 2017;Susick et al. 2020). ...
... Wave-induced forces may have important hydrodynamic consequences to the benthic community even in relatively deep waters (Denny 1987). The role that wave-induced oscillations through the water column may have on biofouling settlement and growth is an important knowledge gap concerning subsurface infrastructure (Navarrete et al. 2020). Earlier studies by Want et al. (2017) reported predictable biofouling assemblages on infrastructure with contrasting wave climates. ...
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A novel system was developed to deploy settlement panels to monitor biofouling growth in situ and evaluate antifouling coatings at depths representative of operational conditions of full-scale marine renewable energy devices. Biofouling loading, species diversity, and succession were assessed at depths ranging from 25-40 m at four tests sites in Orkney (UK) featuring extreme wave and tidal current exposure to more sheltered conditions. Evaluations were carried out over a period of 8 months with intermediate retrieval of samples after 3 months. Early pioneer fouling communities, comprised of colonial hydroids, were succeeded by tube-forming amphipods across sites while solitary tunicates dominated in greater shelter. The highest biofouling loading was observed on high-density polyethylene (HDPE) panels (6.17 kg m −2) compared with coated steel (3.34 kg m −2) panels after 8 months. Distinct assemblages were present at exposed vs sheltered sites. Better understanding of fouling and antifouling strategies may provide guidance to more effectively manage biofouling impacts in this sector.
... Most knowledge about biofouling and the performance of antifouling coatings has been conducted in the laboratory or in situ, in wave-protected habitats, usually in bays and port harbors. One of the main drawbacks related to in situ tests is the scarcity of studies performed under high-energy environmental conditions, such as under moderate and strong wave and current activity, due to logistical and safety-related difficulties in conducting detailed observations [153][154][155]. Since these high wave-energy regions of coastal oceans are becoming increasingly targeted as areas of human activity, such as aquaculture, and as a source of renewable energy, it is critical to improve knowledge about biofouling risks in these environments, as well as the evaluation of novel antifouling surfaces that can be used in the material design of relevant industrial equipment. ...
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Marine biofouling is an undeniable challenge for aquatic systems since it is responsible for several environmental and ecological problems and economic losses. Several strategies have been developed to mitigate fouling-related issues in marine environments, including developing marine coatings using nanotechnology and biomimetic models, and incorporating natural compounds, peptides, bacteriophages, or specific enzymes on surfaces. The advantages and limitations of these strategies are discussed in this review, and the development of novel surfaces and coatings is highlighted. The performance of these novel antibiofilm coatings is currently tested by in vitro experiments, which should try to mimic real conditions in the best way, and/or by in situ tests through the immersion of surfaces in marine environments. Both forms present their advantages and limitations, and these factors should be considered when the performance of a novel marine coating requires evaluation and validation. Despite all the advances and improvements against marine biofouling, progress toward an ideal operational strategy has been slow given the increasingly demanding regulatory requirements. Recent developments in self-polishing copolymers and fouling-release coatings have yielded promising results which set the basis for the development of more efficient and eco-friendly antifouling strategies.
... But these advances have been largely biased towards internal tissue microbiomes, mostly in mammals, and especially in humans (Costello et al., 2012;Tremaroli & Bäckhed, 2012). In marine ecosystems, the study of susceptibility to macroscopic biofouling on artificial surfaces covered by microbial biofilms at sea has been an active focus of research from an applied material science perspective (Salta et al., 2010;Navarrete et al., 2019;Navarrete et al., 2020;Daille et al., 2020;Antunes et al., 2020). Still, in most natural systems, our understanding of the macroscopic and microscopic interactions remains rudimentary, at best. ...
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In marine ecosystems, most invertebrates possess diverse microbiomes on their external surfaces, such as those found in the pedal mucus of grazing gastropods and chitons that aids displacement on different surfaces. The microbes are then transported around and placed in contact with free-living microbial communities of micro and other macro-organisms, potentially exchanging species and homogenizing microbial composition and structure among grazer hosts. Here, we characterize the microbiota of the pedal mucus of five distantly related mollusk grazers, quantify differences in microbial community structure, mucus protein and carbohydrate content, and, through a simple laboratory experiment, assess their effects on integrated measures of biofilm abundance. Over 665 Amplicon Sequence Variants (ASVs) were found across grazers, with significant differences in abundance and composition among grazer species and epilithic biofilms. The pulmonate limpet Siphonaria lessonii and the periwinkle Echinolittorina peruviana shared similar microbiota. The microbiota of the chiton Chiton granosus, keyhole limpet Fissurella crassa, and scurrinid limpet Scurria araucana differed markedly from one another, and form those of the pulmonate limpet and periwinkle. Flavobacteriaceae (Bacteroidia) and Colwelliaceae (Gammaproteobacteria) were the most common among microbial taxa. Microbial strict specialists were found in only one grazer species. The pedal mucus pH was similar among grazers, but carbohydrate and protein concentrations differed significantly. Yet, differences in mucus composition were not reflected in microbial community structure. Only the pedal mucus of F. crassa and S. lessonii negatively affected the abundance of photosynthetic microorganisms in the biofilm, demonstrating the specificity of the pedal mucus effects on biofilm communities. Thus, the pedal mucus microbiota are distinct among grazer hosts and can affect and interact non-trophically with the epilithic biofilms on which grazers feed, potentially leading to microbial community coalescence mediated by grazer movement. Further studies are needed to unravel the myriad of non-trophic interactions and their reciprocal impacts between macro- and microbial communities.
... Nevertheless, while it is true that the surface topography created by the polymer and adhered to the solid surface could have been one of the proximate mechanisms deterring establishment of competent settling larvae, it is also possible that changes in the hydrophobic nature of the polymer also played a role (as shown in other species). In previous work [50], we have shown that larvae of most macro-foulers (barnacles, tunicates) settle abundantly over a very wide range of surface roughness, bracketing the roughness range presented in these experiments. Simple comparison among materials of similar surface roughness will therefore not provide much insight into the mechanisms by which PEDOT deters larval settlement. ...
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Maritime enterprises have long sought solutions to reduce the negative consequences of the settlement and growth of marine biofouling (micro- and macro-organisms) on virtually all surfaces and materials deployed at sea. The development of biofouling control strategies requires solutions that are cost-effective and environmentally friendly. Polymer-based coatings, such as the poly (3,4-ethylenedioxythiophene) (PEDOT) and its potential applications, have blossomed over the last decade thanks to their low cost, nontoxicity, and high versatility. Here, using multiple-choice larval settlement experiments, we assessed the efficacy of PEDOT against the balanoid barnacle Notobalanus flosculus one of the most common biofouling species in Southeastern Pacific shores, and compared results against a commercially available antifouling (AF) coating, and biofilms at different stages of succession (1, 2, 4 and 8 weeks). We show that larval settlement on PEDOT-coated surfaces was similar to the settlement on AF-coated surfaces, while larvae settled abundantly on roughened acrylic and on early-to-intermediate stages of biofilm (one to four weeks old). These results are promising and suggest that PEDOT is a good candidate for fouling-resistant coating for specific applications at sea. Further studies to improve our understanding of the mechanisms of barnacle larval deterrence, as well as exposure to field conditions, are encouraged.
Technical Report
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Marine energy devices are installed in highly dynamic environments and have the potential to affect benthic and pelagic habitats around them. Regulatory bodies often require baseline characterization and/or post-installation monitoring to determine whether changes in these habitats are being observed. However, a great diversity of technologies is available for surveying and sampling marine habitats. Selecting the most suitable instrument to identify and measure changes in habitats at marine energy sites can become a daunting task. We conducted a thorough review of journal articles, survey reports, and grey literature to extract information about the technologies used, the data collection and processing methods, and the performance and effectiveness of these instruments. We examined documents related to marine energy development, offshore wind farms, oil and gas offshore sites, and other marine industries around the world over the last 20 years, as well as national and international guidelines for surveying habitats around offshore activities. A total of 120 different technologies were identified across six main habitat categories: seafloor, sediment, infauna, epifauna, pelagic, and biofouling. The technologies were organized into 12 broad technology classes: acoustic, corer, dredge, grab, hook and line, net and trawl, plate, remote sensing, scrape samples, trap, visual, and others. Visual was the most common and the most diverse technology class, with applications across all six habitat categories. Sampling designs varied considerably among the reviewed studies but transect was the predominant design for surveying seafloor, epifauna, and pelagic habitats. The most common data analyses were univariate and multivariate statistical analyses aimed at calculating and comparing biodiversity indices, characterizing faunal assemblages or sediment classes, or modeling the distribution of animals related to abiotic parameters. Technologies and sampling methods adaptable and designed to work efficiently in energetic environments have greater success at marine energy sites. In addition, sampling designs and statistical analyses should be carefully thought through to identify differences in faunal assemblages and spatiotemporal changes in habitats.
Full-text available
Marine energy devices are installed in highly dynamic environments and have the potential to affect the benthic and pelagic habitats around them. Regulatory bodies often require baseline characterization and/or post-installation monitoring to determine whether changes in these habitats are being observed. However, a great diversity of technologies is available for surveying and sampling marine habitats, and selecting the most suitable instrument to identify and measure changes in habitats at marine energy sites can become a daunting task. We conducted a thorough review of journal articles, survey reports, and grey literature to extract information about the technologies used, the data collection and processing methods, and the performance and effectiveness of these instruments. We examined documents related to marine energy development, offshore wind farms, oil and gas offshore sites, and other marine industries around the world over the last 20 years. A total of 120 different technologies were identified across six main habitat categories: seafloor, sediment, infauna, epifauna, pelagic, and biofouling. The technologies were organized into 12 broad technology classes: acoustic, corer, dredge, grab, hook and line, net and trawl, plate, remote sensing, scrape samples, trap, visual, and others. Visual was the most common and the most diverse technology class, with applications across all six habitat categories. Technologies and sampling methods that are designed for working efficiently in energetic environments have greater success at marine energy sites. In addition, sampling designs and statistical analyses should be carefully thought through to identify differences in faunal assemblages and spatiotemporal changes in habitats.
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Community assembly may not follow predictable successional stages, with a large fraction of the species pool constituted by potential pioneering species and successful founders defined through lottery. In such systems, priority effects may be relevant in the determination of trajectories of developing communities and hence diversity and assemblage structure at later advanced states. In order to assess how different founder species may trigger variable community trajectories and structures, we conducted an experimental study using subtidal sessile assemblages as model. We manipulated the identity of functionally different founders and initial colony size (a proxy of the time lag before the arrival of later species), and followed trajectories. We did not observe any effects of colony size on response variables, suggesting that priority effects take place even when the time lag between the establishment of pioneering species and late colonizers is very short. Late community structure at experimental panels that started either with the colonial ascidian Botrylloides nigrum, or the arborescent bryozoan Bugula neritina, was similar to control panels allowed natural assembling. In spite of high potential for fast space domination, and hence negative priority effects, B. nigrum suffered high mortality and did not persist throughout succession. Bugula neritina provided complex physical microhabitats through conspecific clustering that have enhanced larval settlement of late species arrivals, but no apparent facilitation was observed. Differently, panels founded by the encrusting bryozoan Schizoporella errata led to different and less diverse communities compared to naturally assembled panels, evidencing strong negative priority effects through higher persistence and space preemption. Schizoporella errata founder colonies inhibited further conspecific settlement, which may greatly relax intraspecific competition, allowing resource allocation to colony growth and space domination, thus reducing the chances for the establishment of other species.
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Marine aquaculture presents an opportunity for increasing seafood production in the face of growing demand for marine protein and limited scope for expanding wild fishery harvests. However, the global capacity for increased aquaculture production from the ocean and the relative productivity potential across countries are unknown. Here, we map the biological production potential for marine aquaculture across the globe using an innovative approach that draws from physiology, allometry and growth theory. Even after applying substantial constraints based on existing ocean uses and limitations, we find vast areas in nearly every coastal country that are suitable for aquaculture. The development potential far exceeds the space required to meet foreseeable seafood demand; indeed, the current total landings of all wild-capture fisheries could be produced using less than 0.015% of the global ocean area. This analysis demonstrates that suitable space is unlikely to limit marine aquaculture development and highlights the role that other factors, such as economics and governance, play in shaping growth trajectories. We suggest that the vast amount of space suitable for marine aquaculture presents an opportunity for countries to develop aquaculture in a way that aligns with their economic, environmental and social objectives.
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As part of ongoing commitments to produce electricity from renewable energy sources in Scotland, Orkney waters have been targeted for potential large-scale deployment of wave and tidal energy converting devices. Orkney has a well-developed infrastructure supporting the marine energy industry; recently enhanced by the construction of additional piers. A major concern to marine industries is biofouling on submerged structures, including energy converters and measurement instrumentation. In this study, the marine energy infrastructure and instrumentation were surveyed to characterise the biofouling. Fouling communities varied between deployment habitats; key species were identified allowing recommendations for scheduling device maintenance and preventing spread of invasive organisms. A method to measure the impact of biofouling on hydrodynamic response is described and applied to data from a wave-monitoring buoy deployed at a test site in Orkney. The results are discussed in relation to the accuracy of the measurement resources for power generation. Further applications are suggested for future testing in other scenarios, including tidal energy.
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Marine aquaculture is expanding into deeper offshore environments in response to growing consumer demand for seafood, improved technology, and limited potential to increase wild fisheries catches. Sustainable development of aquaculture will require quantification and minimization of its impacts on other ocean-based activities and the environment through scientifically informed spatial planning. However, the scientific literature currently provides limited direct guidance for such planning. Here, we employ an ecological lens and synthesize a broad multidisciplinary literature to provide insight into the interactions between offshore aquaculture and the surrounding environment across a spectrum of spatial scales. While important information gaps remain, we find that there is sufficient research for informed decisions about the effects of aquaculture siting to achieve a sustainable offshore aquaculture industry that complements other uses of the marine environment.
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Like plants, sessile invertebrates are often morphologically modified at high densities. At high densities acorn barnacles commonly form hummocks of tall, densely packed individuals. We examined hummock development and its consequences on filter feeding and growth in the northern acorn barnacle, Sernibalunus bala~zoides. Hummocking occurs in response to high recruitment densities and growth rates, which intensify competition for primary substrate space. In the field, hummocks are more common at low than at high tidal heights, paralleling within-site variation in recruitment and growth rates. Hummocks also are most pronounced at high-flow sites with high barnacle recruitment and growth rates. In laboratory flume studies, particle capture rates were higher for individuals near the peak of hummocks than for solitary individuals, and were lower for individuals in the troughs between hummocks than for solitary individuals. Hummocked individuals are el- evated above the surface and are thus likely exposed to higher particle fluxes than either individuals between hummocks or solitary individuals. These patterns in particle capture closely match patterns of barnacle shell growth in the field. The shells of hummocked individuals were larger than those of solitary individuals, which were larger than the shells of individuals in troughs between hummocks. The tissue growth of solitary individuals, however, is lower than that of crowded individuals, apparently since, without neighbors, solitary barnacles must allocate more resources to structural support than do crowded individuals. Thus, crowding may benefit individual barnacles by reducing their skeletal support costs. Most studies of crowding in sessile, space-limited invertebrates have focused on neg- ative, competitive effects. Our results, along with previous work showing that crowding may benefit northern acorn barnacles by buffering them from heat and desiccation stress and increasing reproductive output, illustrate that crowding also can positively affect sessile organisms. We suggest that interactions among most sessile, space-limited invertebrates are best viewed as a balance between negative and positive effects.
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Phytoplankton and particulate organic matter constitute the primary food source for adult filter-feeders, as well as for larval stages of many benthic and pelagic organisms. The structure and dynamics of nearshore benthic communities may be associated with variation in nearshore primary production. However, we know little about the scales of variability in phytoplankton in nearshore waters along open coasts, or about their causes. To characterize spatial and temporal patterns of chl a concentration, we conducted 2.5 yr of daily, shore-based monitoring at 3 sites separated by 10s of km within an upwelling region in central Chile. We found that: (1) peaks in chl a concentration were typically short-lived, persisting no longer than 4 d, (2) blooms occurred in spring to early summer months at all sites, but also during autumn months at 1 site (Las Cruces), and (3) the intensity and frequency of blooms were consistently different among sites; highest concentrations were at Las Cruces, lower at El Quisco, and the lowest at Quintay. Analyses of wind data and surface temperature, and inspection of Advanced Very High Resolution Radiometer (AVHRR) satellite images, suggested that among-site differences were due, at least in part, to alongshore variation in upwelling intensity and the formation of warm-water pockets or upwelling shadows in sections of the coast, such as Las Cruces. In contrast to the spatial pattern described offshore and over larger spatial scales, chl a concentrations were significantly lower at the coldest site, Quintay, located at the core of an upwelling center (Pta. Curaumilla), than at the warmer site of Las Cruces, which lies downstream from upwelling. Day-to-day variation in chl a levels during spring at Las Cruces seems related to the alongshore intrusion of waters upwelled upstream. Overall, the pattern observed at our 3 sites, together with previous studies at other upwelling systems, suggests that sections of the coast around 15 to 20 km downstream (equatorward) from upwelling centers could exhibit consistently higher phytoplankton concentrations than sites located in front of upwelling centers, generating a source-sink type of geographic pattern of nearshore nutrients and phytoplankton along the coast.
Antifouling paint particles (APPs) are generated during periodical maintenance of boat hulls. Chemical composition and toxicity (either chronic or acute) of APPs found in the sediment was evaluated using the epibenthic copepod Nitokra sp. The APPs analyzed showed the presence of high levels of metals such as Cu (234,247 ± 268 μg g⁻¹), Zn (112,404 ± 845 μg g⁻¹) and the booster biocide DCOIT (0.13 μg g⁻¹). Even at low concentrations (as from 5 mg g⁻¹ of APPs by mass of sediment) a significantly decrease in the fecundity was observed in laboratory tests. When the sediment was disturbed in elutriate test, a LC50 of 0.14% for APPs was found. This study was the first assessment of toxicity associated with the presence of APPs in sediment to benthic organisms, and it calls attention to the need of improving regulations in boatyards and marina areas.
Many landscapes are characterized by a mosaic of patches in various stages of succession. Whether successional paths dampen, track, or magnify extrinsic variation in initial conditions influences how much historical and site-specific detail is required to explain variation in patch composition. I investigated the patterns and importance of historical effects in a successional marine rocky intertidal community on the central coast of Oregon, USA Patches in the mid-intertidal mussel bed (M. californianus) were manually cleared in a way that mimicked natural disturbances. In four separate blocks (large patches ~9 m2), three sets of plots were initiated with their starting dates staggered by one year. Within each set of plots, I manipulated the presence/absence of two groups of early successional sessile species under each of three predator densities. This design allowed me to address the following general questions: (1) What are the separate and interactive effects of successional age, yearly variation, and initial conditions on the temporal changes observed after disturbance? (2) When do interactions between early species act to dampen or magnify natural variation between years or starting dates? Succession in mid-intertidal patches in the mussel bed displayed complex patterns of historical effects, which varied among species and between different stages of succession. Embedded in this potential complexity were some consistent and repeatable successional trends. Some potentially important canalizing, or 'noise-dampening' forces in this system included: (1) physiological and/or life history trade-offs between dispersal ability and competitive ability, (2) strong direct biotic interactions, which buffer environmental variability, and (3) compensatory ('buffering') responses of species within an important functional group. 'Noise-amplifying' forces included: (1) variable indirect effects of predators, (2) prey size escapes, and (3) predator saturation (or prey 'Swamp' escapes). Understanding the patterns and causes of consistency or contingency in succession will be critical for managing variability in landscapes that are increasingly dominated by anthropogenic disturbance regimes.
Temperature variability under different wind conditions and its association with the spatial pattern of settlement of three intertidal barnacles – the chthamaloids Jehlius cirratus and Notochthamalus scabrosus, and the balanoid Notobalanus flosculus – were studied across Cartagena Bay, located in the upwelling region of central Chile. During days of strong winds, the diurnal signal in surface temperature at the protected end of the bay (site CTGN) was attenuated and decoupled from the northern sites (ECIM and PCHC) which are directly exposed to wind forcing, suggesting that wind intensity drives shifts in the relative importance of physical transport processes across the bay. Overall, the mean settlement rates of both chthamaloids were higher at PCHC, whereas N. flosculus settled at higher rates in CTGN. Under strong wind conditions, settlement rates of both chthamaloids decreased at the northern sites, while the settlement of N. flosculus reached minima at all three sites. Moreover, the effect of wind stress on the spatial pattern of settlement across the bay differed between species. A significant and positive correlation between the spatial heterogeneity of settlement and maximum daily wind stress – used as a metric for the intensity of the afternoon sea breeze – was found only for J. cirratus. It is concluded that daily changes in wind stress have a strong effect on the spatial pattern of diurnal temperature fluctuations, and on the spatial pattern of barnacle settlement around the bay. Such association emerges from the effect of wind on near-shore circulation and its differential modulation of thermal structure around an open embayment and, by extension, on the patterns of larval transport and onshore delivery to sites located at extremes of the bay, which probably is common in other bays with similar characteristics.