Article

Anticancer and Antioxidant Property of Bunium bulbocastanum Fruits Various Fractions

Authors:
  • College of Pharmaceutical Sciences, Dayananda Sagar University, Bangalore
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Abstract

Bunium bulbocastanum (family Apiaceae) fruits are normally used as a culinary spice for years. Since antioxidant property has a significant role in preventing oxidative stress, which has been demonstrated to be a major cause in the pathogenesis of cancer, the present study was aimed to evaluate the in vitro antioxidant and cytotoxic effect of Bunium bulbocastanum fruit extracts. Standard in vitro antioxidant assays such as reducing power, super oxide scavenging, nitric oxide scavenging and lipid peroxidation were performed. Anticancer activity was demonstrated using MTT assay on human cell lines. Our results indicated that extract of ethyl acetate has more antioxidation potential than the standard (ascorbic acid). Ethyl acetate, aqueous, crude methanolic, n-hexane and chloroform fractions had 31.24, 26.07, 20.67, 15.51 and 5.72% antioxidation potential at 0.5 mg/ml respectively. Moreover, in the screening of anticancer activity MTT assay revealed the potency of ethyl acetate fraction as better anticancer agent concluding the anticancer activity is due to its antioxidant property.

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... B. bulbocastanum (BB), also known as Kala jeera, belongs to the Apiaceae family. Its fruit and seeds have long been used as a spice in cooking [8,9]. BB essential oil is abundant in oxygenated monoterpenes, specificallyterpinene, cumin aldehyde, cymene, and limonene, all of which have antibacterial and antioxidant qualities [10,11]. ...
... As a few studies have shown that various parts of the plant possess antioxidant activity, the seeds were extracted with ethanol and subjected to further fractionation. According to a previous study, ethyl acetate and an aqueous fraction of the plant exhibit antiproliferative activity against Hep2, K-562, MCF7, and NIH3T3 cancer cell lines [8]. Preliminary phytochemical studies showed that the ethyl acetate seed fraction contains terpenoids, tannins, and steroids ( Table 2). ...
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Several in vitro investigations of the therapeutic characteristics of B. bulbocastanum have shown that it has cytotoxic, antifungal, and antibacterial effects. It also exhibits antioxidant and anticancer effects. When the ethyl acetate fraction of B. bulbocastanum was examined for its phytochemical composition, it was found to be rich in phenolic compounds and had significant cytotoxic effects on PC-3 cell lines. The prostate length-to-weight ratio was significantly higher in vivo in the ethyl acetate fraction-treated group. Compared to the disease control group, his-topathological examination of the ethyl acetate-treated group revealed a reduction in inflammation and malignant lesions, confirming its antiproliferative efficacy. According to serum biochemistry, acid phosphatase and PSA levels in the ethyl acetate fraction treatment group were significantly lower than those in the disease control group. When compared to the disease control group, malondialdehyde levels in the ethyl acetate fraction treatment group were likewise reduced dramatically. However, in the group treated with the ethyl acetate fraction, glutathione levels increased considerably. The ethyl acetate fraction of B. bulbocastanum may have cytotoxic and antiproliferative potential, both in vitro and in vivo.
... Moreover, results of Hazarika and Das [78] suggests that all fractions of B. bulbocastanum extract (aqueous, crude methanolic, ethyl acetate, n-hexane and chloroform) had antioxidation potential with an encouraging finding of percent antioxidation inhibition. ...
... From the investigation of Hazarika and Das [78] using MTT assay, we are able to conclude that aqueous and ethyl acetate extract of B. bulbocastanum fruit has a noteworthy anticancer activity. ...
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The purpose of this review is to provide comprehensive scientific information on Talghouda (Bunium fontanesii), the geographical distribution, botanical description, its chemistry, pharmacological properties of species are stated. As much data on B. fontanesii was gathered using several scientific search engines as: Google Scholar, Science Direct, PubMed, SpringerLink, Web of Science and Scopus. In this review, studies mentioned B. fontanesii and related synonyms and its phytopharmacological activities were cited, the data have been classified according to the ethnomedical use, the phytochemistry and the biological activities of Talghouda. Previous ethnobotanical studies have reported that Talghouda has been used in many folk medicines, especially in thyroid dysfunction, their essential oils and seeds are used in foods in the form of bread. Compositional analysis in different parts of B. fontanesii shows different classes of compounds, including terpenoids and non-terpenoids, as caryophyllene oxide and caryophyllene, (Z)-farnesene, germacrene B, spathulenol, nonacosane and palmitic acid. Phenolic compounds, fatty acids and alkaloids. Polar and non-polar extracts like essential oils have a wide variety of pharmacological activities, i.e., antioxidant, antifungal, antibacterial, anti-inflammatory, anticancer, enzyme inhibition, antiglycation, phytotoxic activity, hypoglycemic effect, estrogenic effects activities. This review confirms the important ethnobotanical and pharmacological potential of Talghouda.
... Moreover, results of Hazarika and Das [78] suggests that all fractions of B. bulbocastanum extract (aqueous, crude methanolic, ethyl acetate, n-hexane and chloroform) had antioxidation potential with an encouraging finding of percent antioxidation inhibition. ...
... From the investigation of Hazarika and Das [78] using MTT assay, we are able to conclude that aqueous and ethyl acetate extract of B. bulbocastanum fruit has a noteworthy anticancer activity. ...
... The antioxidant capabilities, anti-inflammatory activity, antiproliferative, and antiplatelet actions of quercetin can all be ascribed to its anti-atherogenic activity. Antioxidant, anti-artherogenic, anti-inflammatory, anti-carcinogenic, neuroprotective, antibacterial, and antiviral effects have been discovered in it [5] . Quercetin also has GRAS (Generally Recognized As Safe) status. ...
... [10,11] Moreover compound with anticancer agents are reported to have a potent antioxidant property. [12,13] With an intention of discovering a better anticancer agent and antioxidant a series of possible compounds have been prepared on, N 10 acridones by Ulmann's condensation method. ...
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