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Portable Water Bath to Support Nanofibrils Processing

Authors:
Warji Warji at Lampung University
Warji Warji
Nanik Purwanti at IPB University
Nanik Purwanti
Sutrisno Suro Mardjan at IPB University
Sutrisno Suro Mardjan
Sri Yuliani at National Research and Innovation Agency
Sri Yuliani
  • National Research and Innovation Agency
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Abstract and Figures

Nanofibrils are nano-sized fibrils made from protein isolates. To make the nanofibrils that protein isolate solution was heated in a water bath at 80 C while stirring for more 16 h. This nanofibril formation process requires a water bath that can heat and stir protein isolates. So far there have been a lot of stirrers and water baths, but those that can heat and stir together simultaneously are very limited. Therefore it is necessary to design a water bath that can be paired with a stirrer so that it can heat and stir continuously. The portable water bath designed includes the chamber, heater, thermostat and control panel. This water bath works in a temperature range of 50 C -100 C. Water bath can be paired with heater.
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Portable Water Bath to Support Nanofibrils Processing
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The 3rd International Symposium on Agricultural and Biosystem Engineering
IOP Conf. Series: Earth and Environmental Science 355 (2019) 012086
IOP Publishing
doi:10.1088/1755-1315/355/1/012086
1
Portable Water Bath to Support Nanofibrils Processing
Warji1, N Purwanti2, Sutrisno2 and S Yuliani3
1Agricultural Engineering, Lampung University, Lampung, Indonesia
2Biosystem Engineering Division, Department of Mechanical and Biosystem
Engineering, Bogor Agricultural University, Bogor, Indonesia
3IndonesianCenter for Agricultural Postharvest Research and Development, Ministry
of Agriculture of Republic of Indonesia, Cimanggu Agricultural Research Campus,
Bogor, Indonesia.
Email: warji1978@gamail.com
Abstract. Nanofibrils are nano-sized fibrils made from protein isolates. To make the nanofibrils
that protein isolate solution was heated in a water bath at 80 oC while stirring for more 16 h.
This nanofibril formation process requires a water bath that can heat and stir protein isolates. So
far there have been a lot of stirrers and water baths, but those that can heat and stir together
simultaneously are very limited. Therefore it is necessary to design a water bath that can be
paired with a stirrer so that it can heat and stir continuously. The portable water bath designed
includes the chamber, heater, thermostat and control panel. This water bath works in a
temperature range of 50oC -100oC. Water bath can be paired with heater.
1. Introduction
Nanofibrils is globular proteins including whey proteins, soy proteins and egg white proteins self-
assemble into fibrillar structures with several nanometers thickness and several micrometers length by
prolonged heating at very acidic conditions [1-7]. Nano fibrils formed long and straight with a few
nanometers of diameter, as previously reported by [5], [8], and [9].
Nano fibrils is biomaterial that can formed shell of microcapsules [8-10]. Ther nanofibrils potential
as gelling agents and thickener in food; e.g. juice and pudding. The Nano fibrils also hold an opportunity
to enhance food texture. Nanofibrils were formed from soy isolate protein (SPI) or whey protein isolate
(WPI). This isolate protein solutions heated in a water bath at 80 oC while stirring for 16 h. So, portable
water bath that paired with stirer is needed in this process. This research aim to design portable water
bath to support nanofibrils processing.
2. Materials and Methods
2.1. Materials
Materials used in design were chamber, heater bar, electric swicth, thermostat, indicator lamp, stecker
and cable (Fig.1). Material used in nanofibril processing is soy protein isolate, HCl 37% and double
distilled water.
The 3rd International Symposium on Agricultural and Biosystem Engineering
IOP Conf. Series: Earth and Environmental Science 355 (2019) 012086
IOP Publishing
doi:10.1088/1755-1315/355/1/012086
2
Figure 1. thermostat. Figure 2. heater bar. Figure 3.swicth and lamp indicator
2.2. Preparation of protein nanofibrils
SPI was formed into nanofibrils following the method of [9] and [10]. SPI suspensions were prepared
by dispersing 2 %w/w of the proteins in double distilled water. The protein suspensions were stirred
overnight to complete hydration and then, the pH was set to 2.0 using 6 N HCl solution. The protein
suspensions at this pH were then heated in a water bath at 80 oC while stirring for 16 h (figure 2).
Figure 4. Nanofibrils preparation
3. Results and Discussion
3.1. Portable water bath
Portable water bath show in figure 3. The Portatble water bath consist of chamber, heater bar, themostat,
swicth and indicator lamp. The chamber is made of non-magnetic stainless so that it does not interfere
with the stiter process using a magnetic bar. Thermostats can be setup in a temperature range of 50 oC
to 300 0C. In water material portable water bath can work in a temperature range of 50 oC to 100 oC;
while in media with boiling points above 100 oC, this water bath can work in the range of 50 oC to the
boiling point of the material.
Figure 5. Portable water bath.
Portable water bath can be paired with magnetic stirers (figure 4a). The test results show that the
water bath can heat water in the chamber. The time needed to heat 3 liters to 80 oC for about 15 minutes.
The heater set at 83 oC can raise the water temperature in the chamber portable water bath to a
temperature of 83 oC and the heater off at that temperature. Heatar on at 78 oC (figure 4b).
a
b
c
The 3rd International Symposium on Agricultural and Biosystem Engineering
IOP Conf. Series: Earth and Environmental Science 355 (2019) 012086
IOP Publishing
doi:10.1088/1755-1315/355/1/012086
3
Figure 6. Heating process, portable Figure 7. heating temperature graph.
water bath paired on magnetic stirer.
3.2. Nanofibrils
SPI can be converted into SPI nanofibrils through a heating process in a water bath at 80oC and stirred
for 16 hours.
Figure 8. SPI suspension Figure 9. SPI nanofibrils image that modified from
[9] (b)
The results of the observations using TEM show nanofibrils in the form of curve curves as the results
of the research by [9].
4. Conclusion
The portable water bath designed includes the chamber, heater, thermostat and control panel. This water
bath works in a temperature range of 50oC -100oC. Water bath can be paired with heater.
5. References
[1] Moayedzadeh S, Madadlou S and Khosrowshahi A 2015 Formationmechanisms,
handlinganddigestibility of foodproteinnanofibrils Trends in Food Science & Technology. 45:
50-59
[2] Bolder S G, Hendrickx H, Sagis L M C and van der Linden E 2006 Fibril assemblies in aqueous
whey protein mixtures Journal of Agricultural and Food Chemistry. 54: 4229-4234
0
10
20
30
40
50
60
70
80
90
010 20 30
Temperature (oC)
Time (minute)
a
b
a
The 3rd International Symposium on Agricultural and Biosystem Engineering
IOP Conf. Series: Earth and Environmental Science 355 (2019) 012086
IOP Publishing
doi:10.1088/1755-1315/355/1/012086
4
[3] Akkermans C, van der Goot A J, Venema P, Gruppen J M and Boom R M.2007 Micrometer-sized
fibrillar protein aggregates from soy glycinin and soy protein isolate Journal of Agricultural
and Food Chemistry. 5: 9877-9882
[4] Akkermans C, van der Goot A J, Venema P, der Linden V and Boom R M. 2008 Formation of
fibrillar whey protein aggregates: Influence of heat and shear treatment, and resulting rheology
Food Hydrocolloids. 22:1315–1325
[5] Sagis L M C, de Ruiter R, Rossier-Miranda F J, de Ruiter J, Schroën K, van Aelst A C, Kieft H,
Boom R and van der Linden E 2008 Polymer microcapsules with a fiber-reinforced
nanocomposite shell Langmuir. 24:1608-1612
[6] Humblet-Hua K N P, Scheltens G, van der Linden E, Sagis L M C 2010 Encapsulation systems
based on ovalbumin fibrils and high methoxyl pectin Food Hydrocolloids. 25:307-314.
[7] Warji, Mardjan S S, Yuliani S, K Schroën, Purwanti N 2018 Flow Behavior of Isolate Protein
from Soybeans var. Grobogan and Whey Protein Isolate at Acidic Condition under Various
Heating Times Jurnal Keteknikan Pertanian. 2:171-178 DOI: 10.19028/jtep.06.2.171-178
[8] Rossier-Miranda F J, Schroën K, Boom R 2010 Mechanical characterization and pH response of
fibril-reinforced microcapsules prepared by layer-by-layer adsorption Langmuir. 26:19106-
19113
[9] Warji, Mardjan S S, Yuliani S, Purwanti N 2017 Characterization of nanofibrils from soy protein
and their potential applications for food thickener and building blocks of microcapsules
International Journal of Food Properties. 20:sup1 s1121-s1131http://dx.doi.org/
10.1080/10942912.2017. 1336720.
[10] Purwanti N, Warji, Mardjan S S, Yuliani S, K Schroën 2018 Preparation of Multilayered
Microcapsules from Nanofibrils of Soy Protein Isolate using Layer-by-Layer Method. 147:
012009 doi :10.1088/1755-1315/147/1/012009
... Warji и колектив [19] предлагат портативна водна баня за получаване на нанофибрили. ...
... Warji et al. [19] offer a portable water bath to produce nanofibrils. The advantage of the proposed device is that the main vessel of the bath is made of non-magnetic material and the authors use a magnetic stirrer to maintain a homogeneous temperature in the vessel. ...
... В настоящата работа са използвани изходни данни от портативна вана за получаване на нанофибрили, предложена от Warji и колектив [19]. ...
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