A Molecular method for the recovery and identification of enteric virus in shellfish

ArticleinThe New Microbiologica: official journal of the Italian Society for Medical Virology (SIVIM) 26(2):157-162 · March 2003with4 Reads
Impact Factor: 1.78 · Source: OAI

    Abstract

    In this paper we report the results of an investigation into the presence of enteric viruses in shellfish from the waters around Sardinia. Twenty two samples of shellfish were examined using a rapid and sensitive technique to concentrate and detect viral RNA in shellfish tissues. After recovery of viral particles, RNA was extracted, transcribed into cDNA and amplified using "nested PCR". Testing with enterovirus-specific RT-PCR produced positive results in over 13% of specimens. The virus detection procedure appears to be effective. In some circumstances it could be a better test of water quality than conventional monitoring techniques.