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Caffeine Consumption and the Colonic Mucosa-Associated Gut Microbiota: 196

Tuesday, October 29 10:30 AM - 4:00 PM Location: Exhibit Halls 3 and 4 (Street Level)
P1916 - Caffeine Consumption and the Colonic Mucosa-Associated Gut Microbiota
Award: Fellows-in-Training Award (Colon Category)
Award: Presidential Poster Award
Presenting Author(s)
Shawn Gurwara, MD , Annie Dai , Nadim Ajami, PhD , Hashem B. El-Serag, MD, MPH
MACG , Li Jiao, MD
Baylor College of Medicine, Houston, TX; MicrobiomeDX, Houston, TX; Baylor College of Medicine and Center
for Innovations in Quality, Effectiveness and Safety (IQuESt) / Michael E. DeBakey Veterans Affairs Medical
Center, Houston, TX
Introduction: Studies have demonstrated health benefits of caffeine consumption, including decrease in
cardiovascular disease, diabetes, and liver diseases. The exact mechanisms are not known. Caffeine
consumption may possibly modulate the gut microbiome and therefore affect health and disease risk. We
examined the association between caffeine consumption and the composition and structure of the colonic-gut
Shawn Gurwara, MD
Baylor College of Medicine
Houston, Texas
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Methods: In this study, 34 participants underwent a screening colonoscopy and had endoscopically normal
colons. We obtained a total of 97 snap-frozen colonic mucosa biopsies from various segments of colon from
these individuals. Microbial DNA was extracted, and subsequently amplified for the 16S rRNA gene V4 region
and sequenced using the Illumina MiSeq platform. We analyzed the sequencing data using the UPARSE and
SILVA database for operational taxonomic unit (OTU) classification. Self-administered BLOCK Food
Frequency Questionnaire was used to ascertain daily caffeine consumption. We compared the diversity and
relative abundance of bacterial taxonomies by high (≥ 82.9 mg) vs. low (< 82.9 mg) consumption of caffeine.
False discover rate (FDR) P-values were reported and < 0.05 indicated statistical significance.
Results: The alpha diversity was the greatest in high caffeine consumers (Shannon index
beta diversity differed significantly between high vs. low caffeine drinkers (P = 0.0001). The composition of
microbiomes did not differ at the phylum level based on caffeine consumption. At the genus level, high caffeine
consumption was associated with increased relative abundance of Faecalibacterium (P
Roseburia (P= 0.02), but decreased levels of Erysipelatoclostridium (P value < 0.001) and an OTU belonging
to the Lachnospiraceae family (Unc8895) (P< 0.0005). The observed association was seen regardless of age
and dietary quality. Other bacteria commonly detected in gut microbiomes, including Odoribacter
Fusicatenibactor, Alistipes, Blautia, and multiple members of Lachnospiraceae, were significantly more
abundant (P < 0.05) in participants with higher caffeine consumption (Table 1).
Discussion: Higher caffeine consumption was associated with increased richness and evenness of the
mucosa-associated gut microbiota, and higher relative abundance of anti-inflammatory bacteria, such as
Faecalibacterium and Roseburia and lower levels of potentially harmful Erysipelatoclostridium
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Table 1. Relative abundance of genera according to coffee consumption status (FDR P Values < 0.05)
Shawn Gurwara indicated no relevant financial relationships.
Annie Dai indicated no relevant financial relationships.
Nadim Ajami indicated no relevant financial relationships.
Hashem El-Serag indicated no relevant financial relationships.
David Graham indicated no relevant financial relationships.
Li Jiao indicated no relevant financial relationships.
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ACG 2019 Annual Meeting
Citation: Shawn Gurwara, MD; Annie Dai; Nadim Ajami, PhD; Hashem B. El-Serag, MD, MPH; David Y. Graham, MD, MACG;
Scientific Meeting Abstracts. San Antonio, Texas: American College of Gastroenterology.
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ACG 2019 Annual Meeting
... Related studies have shown that this effect of caffeine can lead to a decreased transit time of nutrients in the gut, which has been shown to change microbiome composition by affecting water and nutrient availability throughout the gut (Brown et al., 1990;Kashyap et al., 2013). Caffeine has also been previously associated with a richer gut microbiome and may reduce the prevalence of inflammatory bacteria (Gurwara et al., 2019). ...
... Coffee is made up of several components that individually have the potential to change the gut microbiome, including caffeine, polyphenols, and fiber (Gniechwitz et al., 2007;Liang and Kitts, 2015). Previous studies have investigated the effect of coffee on the microbiome, but to our knowledge there has not been extensive research investigating the combined effects of coffee and antibiotics on the gut microbiome (Jaquet et al., 2009;Nakayama and Oishi, 2013;Gurwara et al., 2019). Food and drug interactions are important to study, as they have the potential to impact microbiome composition, function, and host health in ways that cannot necessarily be predicted (Cabral D. et al., 2020;Cabral D. J. et al., 2019). ...
... Thus, the differences observed between caffeinated and decaffeinated coffee may be the result of other components of coffee besides caffeine. In addition, physiological effects of coffee consumption such as decreased transit time of nutrients in the gut could be occurring in vivo leading to changes in microbial abundance (Brown et al., 1990;Kashyap et al., 2013;Gurwara et al., 2019). ...
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The microbiome is essential for host health, and perturbations resulting from antibiotic use can lead to dysbiosis and disease. Diet can be a powerful modulator of microbiome composition and function, with the potential to mitigate the negative effects of antibiotic use. Thus, it is necessary to study the impacts of diet and drug interactions on the gut microbiome. Coffee is a commonly consumed beverage containing many compounds that have the potential to affect the microbiome, including caffeine, polyphenols, and fiber. We supplemented mice with caffeinated and decaffeinated coffee in conjunction with amoxicillin, and used 16S rRNA amplicon sequencing of fecal samples to investigate changes in diversity and composition of the murine fecal microbiome. We found that antibiotics, regardless of coffee supplementation, caused significant disruption to the murine fecal microbiome, enriching for Proteobacteria, Verrucomicrobia, and Bacteroidetes, but reducing Firmicutes. While we found that coffee alone did not have a significant impact on the composition of the fecal microbiome, coffee supplementation did significantly affect relative abundance metrics in mice treated with amoxicillin. After caffeinated coffee supplementation, mice treated with amoxicillin showed a smaller increase in Proteobacteria, specifically of the family Burkholderiaceae. Correspondingly we found that in vitro, Burkholderia cepacia was highly resistant to amoxicillin, and that it was inhibited by concentrations of caffeine and caffeinated coffee comparable to levels of caffeine in murine ceca. Overall, this work shows that coffee, and possibly the caffeine component, can impact both the microbiome and microbiome members during antibiotic exposure.
... (wang, Cai, et al. 2018) white, green and black tea polyphenols in vitro gastrointestinal model Capsules containing 1000 mg green tea, white tea or black tea polyphenolic extract after in vitro digestion, tPP bioaccessibility and antioxidant activity are higher in the colon than in the duodenum, suggesting the gut microbiota could metabolize tPP and enhance beneficial effects in the large intestine. High (≥82.9 mg) vs. low ( (82.9 mg) consumption of caffeine Higher caffeine consumption was associated with increased richness and evenness of the mucosa-associated gut microbiota, and higher relative abundance of anti-inflammatory bacteria, such as Faecalibacterium and Roseburia and lower levels of harmful Erysipelatoclostridium. (Gurwara et al. 2019) tPs from tea flowers in vitro gastrointestinal model 2.0 mg/ml tPs prepared for saliva digestion tPs enhanced the production of sCFas. in vitro fermentation of tPs altered the composition of gut microbiota, specifically in elevating the ratio of Bacteroidetes to Firmicutes and enriching Prevotella. tPs from Fuzhuan tea physiological behavior and the brain activity, it is assumed that the whole tea or individual tea active ingredient can regulate sleep through the intestinal microbiota. ...
Sleep disorders have received widespread attention nowadays, which have been promoted by the accelerated pace of life, unhealthy diets and lack of exercise in modern society. The chemical medications to improve sleep has shown serious side effects and risks with high costs. Therefore, it is urgent to develop efficient nutraceuticals from natural sources to ensure sleep quality as a sustainable strategy. As the second most consumed beverage worldwide, the health-promoting effects of tea have long been widely recognized. However, the modulatory effect of teas on sleep disorders has received much less attention. Tea contains various natural sleep-modulating active ingredients such as L-theanine (LTA), caffeine, tea polyphenols (TPP), tea pigments, tea polysaccharides (TPS) and γ-aminobutyric acid (GABA). This review focuses on the potential influence and main regulating mechanisms of different tea active ingredients on sleep, including being absorbed by the small intestine and then cross the blood-brain barrier to act on neurons in the brain as neurotransmitters, manipulating the immune system and further affect sleep-wake cycle by regulating the levels of cytokines, and controlling the gut microbes to maintain the homeostasis of circadian rhythm. Current research progress and limitations are summarized and several future development directions are also proposed. This review hopes to provide new insights into the future elucidation of the sleep-regulating mechanisms of different teas and their natural active ingredients and the development of tea-based functional foods for alleviating sleep disorders. Highlights• Natural sleep-modulating active ingredients in tea have been summarized.• Influences of drinking tea or tea active ingredients on sleep are reviewed.• Three main regulating mechanisms of tea active ingredients on sleep are explained.• The associations among nervous system, immune system and intestinal microbiota are investigated.• The potential of developing delivery carriers for tea active ingredients is proposed.
... In recent years, the interplay between diet and microbiota has emerged as an important pathological basis for IBS, which requires further investigation [4,13,14]. Moreover, the role of caffeine consumption on microbiome composition has been evaluated in different diseases, but limited studies have assessed the impact of caffeine in the IBS population [15,16]. Thus, in the present study, we aimed to assess the differences in nutrient intake and gut microbiota patterns between IBS and healthy control (HC) groups; meanwhile, we explored the associations between gut microbial community and food components in both IBS and HC groups. ...
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The interplay between diet and gut microbiota has gained interest as a potential contributor in pathophysiology of irritable bowel syndrome (IBS). The purpose of this study was to compare food components and gut microbiota patterns between IBS patients and healthy controls (HC) as well as to explore the associations of food components and microbiota profiles. A cross-sectional study was conducted with 80 young adults with IBS and 21 HC recruited. The food frequency questionnaire was used to measure food components. Fecal samples were collected and profiled by 16S rRNA Illumina sequencing. Food components were similar in both IBS and HC groups, except in caffeine consumption. Higher alpha diversity indices and altered gut microbiota were observed in IBS compared to the HC. A negative correlation existed between total observed species and caffeine intake in the HC, and a positive correlation between alpha diversity indices and dietary fiber in the IBS group. Higher alpha diversity and gut microbiota alteration were found in IBS people who consumed caffeine more than 400 mg/d. Moreover, high microbial diversity and alteration of gut microbiota composition in IBS people with high caffeine consumption may be a clue toward the effects of caffeine on the gut microbiome pattern, which warrants further study.
... Caffeine is initially absorbed in the stomach and small intestine but is further fermented in the colon by gut microbiota (Scheperjans et al., 2015b). Recently, caffeine consumption is reportedly related to the colonic mucosaassociated gut microbiota (Gurwara et al., 2019); long-term coffee intake is associated with fecal microbial composition in humans, and regular consumption of coffee appears to be associated with changes in some intestinal microbiota groups in which caffeine, as the main dietary factor influencing PD development, may play a role (Gonzalez et al., 2020). Intestinal microorganisms also play a role in the metabolism of caffeine as caffeine was degraded in the gut of H. hampei, and that experimental inactivation of the gut microbiota eliminates this activity, suggesting that the detoxification of caffeine in H. hampei is mediated by the insect's gut microbiota (Ceja-Navarro et al., 2015). ...
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Parkinson’s disease (PD) is the second most common neurodegenerative disorder, characterized by dopaminergic neurodegeneration, motor impairment and non-motor symptoms. Epidemiological and experimental investigations into potential risk factors have firmly established that dietary factor caffeine, the most-widely consumed psychoactive substance, may exerts not only neuroprotective but a motor and non-motor (cognitive) benefits in PD. These multi-benefits of caffeine in PD are supported by convergence of epidemiological and animal evidence. At least six large prospective epidemiological studies have firmly established a relationship between increased caffeine consumption and decreased risk of developing PD. In addition, animal studies have also demonstrated that caffeine confers neuroprotection against dopaminergic neurodegeneration using PD models of mitochondrial toxins (MPTP, 6-OHDA, and rotenone) and expression of α-synuclein (α-Syn). While caffeine has complex pharmacological profiles, studies with genetic knockout mice have clearly revealed that caffeine’s action is largely mediated by the brain adenosine A2A receptor (A2AR) and confer neuroprotection by modulating neuroinflammation and excitotoxicity and mitochondrial function. Interestingly, recent studies have highlighted emerging new mechanisms including caffeine modulation of α-Syn degradation with enhanced autophagy and caffeine modulation of gut microbiota and gut-brain axis in PD models. Importantly, since the first clinical trial in 2003, United States FDA has finally approved clinical use of the A2AR antagonist istradefylline for the treatment of PD with OFF-time in Sept. 2019. To realize therapeutic potential of caffeine in PD, genetic study of caffeine and risk genes in human population may identify useful pharmacogenetic markers for predicting individual responses to caffeine in PD clinical trials and thus offer a unique opportunity for “personalized medicine” in PD.
... The effects have recently been shown also in humans subjects, in an a small-scale observational study with 34 participants. 210 Caffeine consumption via FFQ was associated with larger diversity of GM and increased Faecalibacterium spp. and Roseburia spp., though decreasing Erysipelatoclostridium spp. ...
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Alzheimer's disease (AD) has brought a heavy burden to society as a representative neurodegenerative disease. The etiology of AD combines multiple factors, concluding family, gender, head trauma, diseases and social psychology. There are multiple hypotheses explaining the pathogenesis of AD such as β-amyloid (Aβ) deposition and tau hyperphosphorylation, which lead to extracellular amyloid plaques and neurofibrillary tangles in neurons. The existing therapeutic drugs have several disadvantages including single target, poor curative effect, and obvious side effects. Tea contains many bioactive components, such as tea polyphenols (TPP), L-theanine (L-TH), tea pigment, tea polysaccharides and caffeine. The epidemiological investigations have shown that drinking tea can reduce the risk of AD. The mechanisms of tea active ingredients in the prevention and regulation of AD includes reducing the generation and aggregation of Aβ; inhibiting tau aggregation and hyperphosphorylation; inhibiting neuronal apoptosis and regulate neurotransmitters; relieving oxidative stress and neuroinflammation as well as the regulation of intestinal flora. This review summarizes the different signaling pathways that tea active ingredients regulate AD. Furthermore, we propose the main limitations of current research and future research directions, hoping to contribute to the development of natural functional foods based on tea active ingredients in the prevention and treatment of AD.
Yellow tea, a rare type tea from China, has a rich breadth of functional ingredients and benefits the gastrointestinal tract. However, it is not clear whether the yellow tea extract can alleviate constipation. Therefore, we used loperamide-induced constipation in mice to evaluate the effects of yellow tea extract. Fifty Kunming mice were randomly divided into five groups: normal, model, low-dose yellow tea extract, low-dose yellow tea extract prevention group, and high-dose yellow tea extract prevention group. Mice were administered yellow tea extract for 5 weeks followed by loperamide-induced constipation for the final 2 weeks. The results showed that yellow tea extract alleviated constipation symptoms by improving the fecal water content, defecation weight, and gastrointestinal transit rate. Yellow tea extract intervention also protected colon tissue, regulated serum neurotransmitters, and decreased the vasoactive intestinal peptide level. Furthermore, qRT-PCR indicated that yellow tea extract regulated genes associated with the constipation state, raised 5-HT3 and 5-HT4 and reduced AQP3 and AQP4 mRNA expression. Moreover, we found that yellow tea extract changed the gut microbiota composition. Community diversity and richness were increased and principal co-ordinate analysis demonstrated that the yellow tea extract prophylaxis groups differed from the model group. Difference analysis indicated that yellow tea extract increased Roseburia, Lachnospiraceae_UCG-006, and Bifidobacterium and decreased norank_f_Clostridiales_vadinBB60_group, unclassified_o_Bacteroidales, and Bacteroides, which are correlated with constipation. Based on these results, we believe that regular yellow tea consumption can effectively alleviate constipation.
Bei legalen Drogen handelt es sich um Substanzen, die einfach und rechtmäßig erhältlich sind. Der Konsum und Besitz von diesen Substanzen und der Handel mit ihnen sind straffrei und sie können im Supermarkt, am Kiosk oder in der Tankstelle um die Ecke erworben werden. Doch Vorsicht: Legal bedeutet auf keinen Fall, dass diese Substanzen harmlos sind, denn den Körper interessiert das kleine Wörtchen „legal“ kein bisschen, und nur weil diese Suchtmittel ohne strafrechtliche Folgen zu erwerben sind, rechtfertigt dies die negativen Folgen für Gesundheit und Lebensqualität nicht.
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A healthy gut microbiota (GM) is paramount for a healthy lifestyle. Alterations of the GM have been involved in the aetiology of several chronic diseases, including obesity and type 2 diabetes, as well as cardiovascular and neurodegenerative diseases. In pathological conditions, the diversity of the GM is commonly reduced or altered, often toward an increased Firmicutes/Bacteroidetes ratio. The colonic fermentation of dietary fiber has shown to stimulate the fraction of bacteria purported to have beneficial health effects, acting as prebiotics, and to increase the production of short chain fatty acids, e.g. propionate and butyrate, while also improving gut epithelium integrity such as tight junction functionality. However, a variety of phytochemicals, often associated with dietary fiber, have also been proposed to modulate the GM. Many phytochemicals possess antioxidant and anti-inflammatory properties that may positively affect the GM, including polyphenols, carotenoids, phytosterols/phytostanols, lignans, alkaloids, glucosinolates and terpenes. Some polyphenols may act as prebiotics, while carotenoids have been shown to alter immunoglobulin A expression, an important factor for bacteria colonization. Other phytochemicals may interact with the mucosa, another important factor for colonization, and prevent its degradation. Certain polyphenols have shown to influence bacterial communication, interacting with quorum sensing. Finally, phytochemicals can be metabolized in the gut into bioactive constituents, e.g. equol from daidzein and enterolactone from secoisolariciresinol, while bacteria can use glycosides for energy. In this review, we strive to highlight the potential interactions between prominent phytochemicals and health benefits related to the GM, emphasizing their potential as adjuvant strategies for GM-related diseases.
Green and dark tea extract (GTE/DTE) ameliorate chemical induced-colitis in mice; however, the role of gut microbiota on the anti-colitis effects of green and dark tea in mice remains unclear. This study aims to explore the role of modulations in gut microbes mediated by green and dark tea in colitis mice by a fecal microbiota transplantation (FMT). Our results indicated that GTE and DTE (5 mg/kg bodyweight/day for 4 weeks) exhibited prebiotic effects on the donor mice. Moreover, the FMT treatments (transferring the microbiota daily from the 1g/kg bodyweight fecal sample to each recipient) indicated that, compared with the fecal microbiota from the normal diet treated donor mice, the fecal microbiota from the GTE and DTE treated donor mice significantly ameliorate colitis-related symptoms (e.g., loss of bodyweight, colonic inflammation, loss of barrier integrity, and gut microbiota dysbiosis) and downregulated TLR4/MyD88/NF-κB pathway. Collectively, GTE and DTE ameliorate chemical induced-colitis by modulating gut microbiota.
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