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Caffeine Consumption and the Colonic Mucosa-Associated Gut Microbiota: 196

Authors:
Shawn Gurwara
Shawn Gurwara
Shawn Gurwara
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Annie Dai at Baylor College of Medicine
Annie Dai
Nadim Ajami
Nadim Ajami
Nadim Ajami
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Hashem B. El-Serag
Hashem B. El-Serag
Hashem B. El-Serag
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Abstract

INTRODUCTION Studies have demonstrated health benefits of caffeine consumption, including decrease in cardiovascular disease, diabetes, and liver diseases. The exact mechanisms are not known. Caffeine consumption may possibly modulate the gut microbiome and therefore affect health and disease risk. We examined the association between caffeine consumption and the composition and structure of the colonic-gut microbiota. METHODS In this study, 34 participants underwent a screening colonoscopy and had endoscopically normal colons. We obtained a total of 97 snap-frozen colonic mucosa biopsies from various segments of colon from these individuals. Microbial DNA was extracted, and subsequently amplified for the 16S rRNA gene V4 region and sequenced using the Illumina MiSeq platform. We analyzed the sequencing data using the UPARSE and SILVA database for operational taxonomic unit (OTU) classification. Self-administered BLOCK Food Frequency Questionnaire was used to ascertain daily caffeine consumption. We compared the diversity and relative abundance of bacterial taxonomies by high (≥82.9 mg) vs. low (<82.9 mg) consumption of caffeine. False discover rate (FDR) P -values were reported and <0.05 indicated statistical significance. RESULTS The alpha diversity was the greatest in high caffeine consumers (Shannon index P < 0.0001). The beta diversity differed significantly between high vs. low caffeine drinkers ( P = 0.0001). The composition of microbiomes did not differ at the phylum level based on caffeine consumption. At the genus level, high caffeine consumption was associated with increased relative abundance of Faecalibacterium ( P < 0.0005) and Roseburia ( P = 0.02), but decreased levels of Erysipelatoclostridium ( P value <0.001) and an OTU belonging to the Lachnospiraceae family (Unc8895) ( P < 0.0005). The observed association was seen regardless of age and dietary quality. Other bacteria commonly detected in gut microbiomes, including Odoribacter , Dialister, Fusicatenibactor , Alistipes, Blautia , and multiple members of Lachnospiraceae, were significantly more abundant ( P < 0.05) in participants with higher caffeine consumption (Table 1). CONCLUSION Higher caffeine consumption was associated with increased richness and evenness of the mucosa-associated gut microbiota, and higher relative abundance of anti-inflammatory bacteria, such as Faecalibacterium and Roseburia and lower levels of potentially harmful Erysipelatoclostridium .
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Tuesday, October 29 10:30 AM - 4:00 PM Location: Exhibit Halls 3 and 4 (Street Level)
P1916 - Caffeine Consumption and the Colonic Mucosa-Associated Gut Microbiota
Award: Fellows-in-Training Award (Colon Category)
Award: Presidential Poster Award
Presenting Author(s)
Shawn Gurwara, MD , Annie Dai , Nadim Ajami, PhD , Hashem B. El-Serag, MD, MPH
MACG , Li Jiao, MD
Baylor College of Medicine, Houston, TX; MicrobiomeDX, Houston, TX; Baylor College of Medicine and Center
for Innovations in Quality, Effectiveness and Safety (IQuESt) / Michael E. DeBakey Veterans Affairs Medical
Center, Houston, TX
Introduction: Studies have demonstrated health benefits of caffeine consumption, including decrease in
cardiovascular disease, diabetes, and liver diseases. The exact mechanisms are not known. Caffeine
consumption may possibly modulate the gut microbiome and therefore affect health and disease risk. We
examined the association between caffeine consumption and the composition and structure of the colonic-gut
microbiota.
Shawn Gurwara, MD
Baylor College of Medicine
Houston, Texas
1 1 2
1 1
1 2 3
SG
Page 1 of 4
ACG 2019 Annual Meeting
12/2/2019
https://eventscribe.com/2019/ACG/fsPopup.asp?Mode=presInfo&PresentationID=593977
Methods: In this study, 34 participants underwent a screening colonoscopy and had endoscopically normal
colons. We obtained a total of 97 snap-frozen colonic mucosa biopsies from various segments of colon from
these individuals. Microbial DNA was extracted, and subsequently amplified for the 16S rRNA gene V4 region
and sequenced using the Illumina MiSeq platform. We analyzed the sequencing data using the UPARSE and
SILVA database for operational taxonomic unit (OTU) classification. Self-administered BLOCK Food
Frequency Questionnaire was used to ascertain daily caffeine consumption. We compared the diversity and
relative abundance of bacterial taxonomies by high (≥ 82.9 mg) vs. low (< 82.9 mg) consumption of caffeine.
False discover rate (FDR) P-values were reported and < 0.05 indicated statistical significance.
Results: The alpha diversity was the greatest in high caffeine consumers (Shannon index
beta diversity differed significantly between high vs. low caffeine drinkers (P = 0.0001). The composition of
microbiomes did not differ at the phylum level based on caffeine consumption. At the genus level, high caffeine
consumption was associated with increased relative abundance of Faecalibacterium (P
Roseburia (P= 0.02), but decreased levels of Erysipelatoclostridium (P value < 0.001) and an OTU belonging
to the Lachnospiraceae family (Unc8895) (P< 0.0005). The observed association was seen regardless of age
and dietary quality. Other bacteria commonly detected in gut microbiomes, including Odoribacter
Fusicatenibactor, Alistipes, Blautia, and multiple members of Lachnospiraceae, were significantly more
abundant (P < 0.05) in participants with higher caffeine consumption (Table 1).
Discussion: Higher caffeine consumption was associated with increased richness and evenness of the
mucosa-associated gut microbiota, and higher relative abundance of anti-inflammatory bacteria, such as
Faecalibacterium and Roseburia and lower levels of potentially harmful Erysipelatoclostridium
Page 2 of 4
ACG 2019 Annual Meeting
12/2/2019
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Table 1. Relative abundance of genera according to coffee consumption status (FDR P Values < 0.05)
Disclosures:
Shawn Gurwara indicated no relevant financial relationships.
Annie Dai indicated no relevant financial relationships.
Nadim Ajami indicated no relevant financial relationships.
Hashem El-Serag indicated no relevant financial relationships.
David Graham indicated no relevant financial relationships.
Li Jiao indicated no relevant financial relationships.
Page 3 of 4
ACG 2019 Annual Meeting
12/2/2019
https://eventscribe.com/2019/ACG/fsPopup.asp?Mode=presInfo&PresentationID=593977
Citation: Shawn Gurwara, MD; Annie Dai; Nadim Ajami, PhD; Hashem B. El-Serag, MD, MPH; David Y. Graham, MD, MACG;
- CAFFEINE CONSUMPTION AND THE COLONIC MUCOSA-ASSOCIATED GUT MICROBIOTA. Program No. P1916.
Scientific Meeting Abstracts. San Antonio, Texas: American College of Gastroenterology.
Page 4 of 4
ACG 2019 Annual Meeting
12/2/2019
https://eventscribe.com/2019/ACG/fsPopup.asp?Mode=presInfo&PresentationID=593977
... Related studies have shown that this effect of caffeine can lead to a decreased transit time of nutrients in the gut, which has been shown to change microbiome composition by affecting water and nutrient availability throughout the gut (Brown et al., 1990;Kashyap et al., 2013). Caffeine has also been previously associated with a richer gut microbiome and may reduce the prevalence of inflammatory bacteria (Gurwara et al., 2019). ...
... Coffee is made up of several components that individually have the potential to change the gut microbiome, including caffeine, polyphenols, and fiber (Gniechwitz et al., 2007;Liang and Kitts, 2015). Previous studies have investigated the effect of coffee on the microbiome, but to our knowledge there has not been extensive research investigating the combined effects of coffee and antibiotics on the gut microbiome (Jaquet et al., 2009;Nakayama and Oishi, 2013;Gurwara et al., 2019). Food and drug interactions are important to study, as they have the potential to impact microbiome composition, function, and host health in ways that cannot necessarily be predicted (Cabral D. et al., 2020;Cabral D. J. et al., 2019). ...
... Thus, the differences observed between caffeinated and decaffeinated coffee may be the result of other components of coffee besides caffeine. In addition, physiological effects of coffee consumption such as decreased transit time of nutrients in the gut could be occurring in vivo leading to changes in microbial abundance (Brown et al., 1990;Kashyap et al., 2013;Gurwara et al., 2019). ...
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... This genus is relatively new, created in 2008 when certain species previously classified as Clostridium and Ruminococcus were reclassified into this genus [31]. Although omega-3 fatty acids [34], caffeine [35], and RS4-resistant starch [36] increase Blautia abundance, these components are not abundant in SONOMONO NATTO POWDER CAPSULES TM . Plichta et al. showed that the metabolic activity of Blautia hydrogenotrophica was activated in the presence of Bifidobacterium bifidum, suggesting the possibility of cross-feeding between B. hydrogenotrophica and Bi. ...
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... Concerning the intestinal microbiome, a study showed that caffeine is directly associated with changes only in some intestinal microbiota groups, such as the Bacteroides group [26]. Another study showed that higher caffeine consumption was associated with increased richness and evenness of the mucosa-associated gut microbiota, and higher relative abundance of anti-inflammatory bacteria, such as Faecalibacterium and Roseburia, and lower levels of potentially harmful Erysipelatoclostridium [27]. Regarding other metabolic disorders, it is known that in per-sons who have not previously consumed caffeine, caffeine intake raises blood pressure in the short term, and affect tolerance develops within a week but may be incomplete in some person results in a modest increase in systolic and diastolic blood pressure. ...
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... (wang, Cai, et al. 2018) white, green and black tea polyphenols in vitro gastrointestinal model Capsules containing 1000 mg green tea, white tea or black tea polyphenolic extract after in vitro digestion, tPP bioaccessibility and antioxidant activity are higher in the colon than in the duodenum, suggesting the gut microbiota could metabolize tPP and enhance beneficial effects in the large intestine. High (≥82.9 mg) vs. low ( (82.9 mg) consumption of caffeine Higher caffeine consumption was associated with increased richness and evenness of the mucosa-associated gut microbiota, and higher relative abundance of anti-inflammatory bacteria, such as Faecalibacterium and Roseburia and lower levels of harmful Erysipelatoclostridium. (Gurwara et al. 2019) tPs from tea flowers in vitro gastrointestinal model 2.0 mg/ml tPs prepared for saliva digestion tPs enhanced the production of sCFas. in vitro fermentation of tPs altered the composition of gut microbiota, specifically in elevating the ratio of Bacteroidetes to Firmicutes and enriching Prevotella. tPs from Fuzhuan tea physiological behavior and the brain activity, it is assumed that the whole tea or individual tea active ingredient can regulate sleep through the intestinal microbiota. ...
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