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The complete mitochondrial genome of Paracolopha morrisoni (Baker, 1919) (Hemiptera: Aphididae)



We have determined the mitochondrial genome of Paracolopha morrisoni (Baker, 1919), a gall-forming aphid collected from Korea. The circular mitogenome of Paracolopha morrisoni is 16,330 bp long including 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNAs, and a single large non-coding region of 932 bp. The base composition was AT-biased (84.9%). Gene order of P. morrisoni is identical to all other aphid mitochondrial genomes. Phylogenetic trees show that P. morrisoni is sister to Eriosoma lanigerum both belonging to tribe. The mitochondrial genome of P. morrisoni will be useful in understanding the genetic backgrounds of the species.
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Mitochondrial DNA Part B
ISSN: (Print) 2380-2359 (Online) Journal homepage:
The complete mitochondrial genome of
Paracolopha morrisoni (Baker, 1919) (Hemiptera:
Jieun Lee, Jonghyun Park, Hyobin Lee, Jongsun Park & Wonhoon Lee
To cite this article: Jieun Lee, Jonghyun Park, Hyobin Lee, Jongsun Park & Wonhoon Lee
(2019) The complete mitochondrial genome of Paracolopha�morrisoni (Baker, 1919) (Hemiptera:
Aphididae), Mitochondrial DNA Part B, 4:2, 3037-3039, DOI: 10.1080/23802359.2019.1666046
To link to this article:
© 2019 The Author(s). Published by Informa
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Published online: 18 Sep 2019.
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The complete mitochondrial genome of Paracolopha morrisoni (Baker, 1919)
(Hemiptera: Aphididae)
Jieun Lee
, Jonghyun Park
, Hyobin Lee
, Jongsun Park
and Wonhoon Lee
Department of Plant Medicine, Gyeongsang National University, Jinju, The Republic of Korea;
InfoBoss Co., Ltd, Seoul, The Republic of
InfoBoss Research Center, Seoul, The Republic of Korea;
Institute of Agriculture & Life Science, Gyeongsang National University,
Jinju, The Republic of Korea
We have determined the mitochondrial genome of Paracolopha morrisoni (Baker, 1919), a gall-forming
aphid collected from Korea. The circular mitogenome of Paracolopha morrisoni is 16,330 bp long includ-
ing 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNAs, and a single large non-coding
region of 932bp. The base composition was AT-biased (84.9%). Gene order of P. morrisoni is identical
to all other aphid mitochondrial genomes. Phylogenetic trees show that P. morrisoni is sister to
Eriosoma lanigerum both belonging to tribe. The mitochondrial genome of P. morrisoni will be useful in
understanding the genetic backgrounds of the species.
Received 2 August 2019
Accepted 16 August 2019
Paracolopha morrisoni;
mitochondrial genome;
Aphididae; Korea
Paracolopha morrisoni is an aphid widely occurring in eastern
Asian countries (China, Japan, and Korea), Unites States
(Maryland, and South Carolina), and Europe (Belgium. Britain,
Italy, and The Netherlands; Si 1985;Malumphy2012). In Asia, P.
morrisoni are known to be heteroecious, altering their hosts
from a primary host Zelkova serrata to the secondary host,
bamboo (Sasa spp.; Si 1985). Outside of Asia on the other
hand, they are only found on roots of bamboo species, both
introduced (Phyllostachys and Pleioblastus spp., native to China)
and native species (Arundinaria gigantea,nativetotheUS).
Since P. morrisoni, in America and Europe, are mostly found in
cultivated bamboos and do not alternate hosts, it has been
believed these aphids originated in east Asia but were intro-
duced overseas hitchhiking international trades of host plants.
This is not completely confirmed, however, since the North
American populations show novel characteristics such as alates
occurringinspring(Si1985). To understand genetic back-
ground of this species, we determined the complete mitochon-
drial genome of P. morrisoni collected from South Korea.
Total DNA of P. morrisoni was extracted from wingless
females collected on Zelkova serrata from Haymang-gun,
Gyeongsangnam-do, Korea in 2019 (355402000 N, 1267608400 E;
the specimen is stored in Gyeongsang National University,
Korea accession number: Coll#JE147) using DNeasy Brood &
Tissue Kit (QIAGEN, Hilden, Germany). Raw sequences obtained
from Illumina HiSeqX at Macrogen Inc., Korea, were filtered by
Trimmomatic 0.33 (Bolger et al. 2014)andde novo assembled
and confirmed by Velvet 1.2.10 (Zerbino and Birney 2008),
SOAPGapCloser 1.12 (Zhao et al. 2011), BWA 0.7.17 (Li et al.
2009), and SAMtools 1.9 (Li 2013). Geneious R11 11.1.5
(Biomatters Ltd., Auckland, New Zealand) was used for annota-
tion based on that of Eriosoma lanigerum (NC_033352). ARWEN
(Laslett and Canb
ack 2008) was used to annotate tRNAs.
Paracolopha morrisoni mitochondrial genome length
(Genbank accession is MN167467) is 16,330 bp and GC ratio
is 15.1%, showing AT-biased. It contains 13 protein-coding
genes, 2 rRNAs, and 22 tRNAs. The tRNAs size ranges from 53
to 73 bp, similar to other aphids (5290 bp). Gene order of
P. morrisoni is identical to that of all other aphid mitoge-
nomes which are apparently the ancestral gene order of all
insects (Wang et al. 2013).
All available complete mitochondrial genomes of 34
aphids including P. morrisoni and Bembisia tabaci
(NC_006279) as an outgroup were aligned by MAFFT 7.388
(Katoh and Standley 2013). Bootstrapped maximum-likelihood
(bootstrap repeat is 1000) and neighbor-joining (bootstrap
repeat is 10,000) trees were constructed using MEGA X
(Kumar et al. 2018). Phylogenetic trees show that all valid
aphid tribes are monophyletic with P. morrisoni in Erisomatini
(Figure 1). However, our tree topologies also presented
Erisomatini grouped with Greenideidae resulting in paraphy-
letic manner of subfamily Eristomatinae, which is not the first
time to be reported (Nov
a et al. 2013). Our mitochon-
drial genome will be a key resource in understanding the
genetic backgrounds and phylogenetic position of
P. morrisoni.
CONTACT Jongsun Park InfoBoss Co., Ltd., 301 Room, Haeun Bldg., 670, Seolleung-ro, Gangnam-gu, Seoul 06088, The Republic of
Korea; Wonhoon Lee Department of Plant Medicine, Gyeongsang National University, Jinjudaero 501, Jinju 52828, The Republic
of Korea
Co-first authors.
ß2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use,
distribution, and reproduction in any medium, provided the original work is properly cited.
2019, VOL. 4, NO. 2, 30373039
Disclosure statement
The authors declare that they have no competing interests.
This research was supported by Research of Animal and Plant
Quarantine Agency of South Korea Fund [1545018425].
Jongsun Park
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Figure 1. Maximum-likelihood (bootstrap repeat is 1000) and neighbor-joining (bootstrap repeat is 10,000) phylogenetic trees of 34 aphids and one whitefly mito-
chondrial genomes: Paracolopha morrisoni (MN167467, this study), Acyrthosiphon pisum (NC_011594), Sitobion avenae (NC_024683), Myzus persicae (NC_029727,
KU877171), Diuraphis noxia (NC_022727), Cavariella salicicola (NC_022682), Schizaphis graminum (NC_006158), Aphis gossypii(NC_024581), Aphis fabae mordvilkoi
(NC_039988), Aphis craccivora (NC_031387, KX447142), Hormaphis betula (NC_029495), Cervaphis quercus (NC_024926), Greenidea psidii (NC_041198), Eriosoma lani-
gerum (NC_033352), Baizongia pistaciae (NC_035314), Nurudea yanoniella (NC_035313, MK435595), Nurudea shiraii (NC_035301), Melaphis rhois (NC_036065),
Nurudea ibofushi (NC_035311), Schlechtendalia chinensis (NC_032386), Schlechtendalia peitan (NC_035302), Nurudea choui (NC_035310), Nurudea meitanensis
(NC_035316), Schlechtendalia elongallis (NC_035315), Schlechtendalia flavogallis (NC_035312), Kaburagia rhusicola ovatirhusicola (MF043985), Kaburagia rhusicola
ensigallis (MF043984), Kaburagia rhusicola ovagallis (MF043986), Kaburagia rhusicola rhusicola (MF043987), Mindarus keteleerifoliae (NC_033410), Appendiseta robiniae
(NC_042165), and Bemisia tabaci (NC_006279) as an outgroup. Phylogenetic tree was drawn based on the maximum-likelihood tree. The numbers above branches
indicate bootstrap support values of maximum-likelihood and neighbor joining phylogenetic tree, respectively.
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