MOLECULAR MARKER BASED GENETIC DIVERSITY ANALYSIS IN RICE (ORYZA SATIVA L.) DOUBLED HAPLOIDS USING RAPD AND ISSR MARKERS

Abstract

The availability of an array of molecular markers allowed comparing the efficiency of two of these marker systems for diversity analysis. In the present investigation, two multiloci markers, RAPD (20 primers) and ISSR (20 primers) and also eight morphometric traits were used to assess the genetic diversity among 38 doubled haploid lines and their parents in rice. Informativeness of the primers was established with the help of statistics such as marker index and mean marker index whereas diversity analysis was done using statistics such as dendrogram and principal component analysis. Though the number of ISSR markers (20.55 per primer) generated was more than the number of RAPD markers (7.8 per primer) the level of polymorphism was found to be more with RAPD markers (46.83%) over ISSR markers (35.30%). All the derived parameters to assess the efficiency of marker systems such as marker index and mean marker index values clearly indicate the RAPD markers were more polymorphic when compared to ISSR markers.