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Data Article
Data on mineral composition, fatty acids,
oxidative stability, UV-VIS spectra and
fluorescence emission of the Dersani
®
and
Sunflower
®
oils used as a cicatrizing agent
Joseane Bortolanza de Oliveira
a
, Flavio Santana Michels
b
,
Elaine Silva de P
adua Melo
a
,
Carlos Eduardo Domingues Naz
ario
c
,
Anderson Rodrigues Lima Caires
b
, Daniel Araujo Gonçalves
d
,
Claudia Andrea Lima Cardoso
e
,
Valter Arag~
ao do Nascimento
a
,
*
a
Group of Spectroscopy and Bioinformatics Applied Biodiversity and Health (GEBABS), School of Medicine,
Federal University of Mato Grosso do Sul, Campo Grande/MS, Brazil, S/N, Campo Grande, 79070-900, Brazil
b
Optics and Photonics Group, Institute of Physics, Federal University of Mato Grosso do Sul, Campo Grande,
Brazil
c
Federal University of Mato Grosso do Sul, Institute of Chemistry, Campo Grande, Brazil
d
Department of Chemistry, Minas Gerais State University eUEMG, Ituiutaba, Brazil
e
Centro de Estudos Em Recursos Naturais, UEMS, Dourados, MS, Brazil
article info
Article history:
Received 2 July 2019
Received in revised form 12 August 2019
Accepted 14 August 2019
Available online 22 August 2019
Keywords:
Essential
Sunflower oil
Stability
Antioxidants
Spectrophotometry
abstract
Dersani
®
and sunflower
®
oils are used by the Brazilian population
as a cicatrizing agent. However, data on physical and chemical
properties of these oils are scarce. In this data article on oils, we
determined a total of 14 fatty acids composition by gas chroma-
tography (GC), as well as quantifying the elements contents (Ca, K,
Mg, Al, Cr, Fe, Mn, Na, P, Se and Zn) using inductively coupled
plasma optical spectrometry (ICP OES). Rancimat method was used
to determine the oxidative stability of the oils at temperature of
110
C, in which the induction times for Dersani
®
and Sunflower
®
oils were 1.54 (±0.02) and 6.21 (±0.17) hours, respectively. Spec-
troscopic techniques UV-VIS and fluorescence were employed to
obtain spectral datasets. UV-VIS and fluorescence spectroscopy
*Corresponding author.
E-mail address: aragao60@hotmail.com (V. Arag~
ao do Nascimento).
Contents lists available at ScienceDirect
Data in brief
journal homepage: www.elsevier.com/locate/dib
https://doi.org/10.1016/j.dib.2019.104427
2352-3409/©2019 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://
creativecommons.org/licenses/by/4.0/).
Data in brief 26 (2019) 104427
reveals the presence of phenolic, tocopherols, tocotrienols and
methyl-linolenate compounds in the oils. The determination of
mineral and others contents in oils is an important criterion for the
assessment of oil quality with regard to oxidation and their
toxicity, properties and storage.
©2019 The Authors. Published by Elsevier Inc. This is an open
access article under the CC BY license (http://creativecommons.
org/licenses/by/4.0/).
Specifications Table
Subject area Biochemistry
More specific subject area Essential fatty acid oil and thermal stability
Type of data Table, Figures
How data was acquired Chromatograph model 3800 (Varian, Walnut Creek, USA);
Microwave digestion System (Speedwave four, Berghof, Eningen, BW, Germany);
ICP OES (iCAP 6300 Duo, Thermo Fisher Scientific, Bremen, Germany);
Rancimat 893 (Metrohm Co, Basel);
Spectrophotometer (Operating Range: 190 - 1100 nm, Lambda265UV/Vis, Perkin Elmer,
Waltham, MA, USA);
Spectrofluorometer (FS-2, Scinco, Seoul, Korea);
Data format Raw, Analyzed
Experimental factors a) An amount of the oil (0.16 g for Dersani
®
and 0.16 g for Sunflower, respectively), was
added in a solution of KOH (5%) in MeOH (4 mL) and heated in a water-bath at 100
C for
5 min. A solution of NH
4
CleH
2
SO
4
in MeOH (5.0 mL) was then added and homogenized
for 30 seconds.
b) Sample Pretreatment with Microwave-Assisted Techniques: Weigh 0.7 g of the each
sample into the digestion vessel. Add 6.0 mL of HNO
3
(65%, Merck - Darmstadt,
Germany) and 2.0 mL of H
2
O
2
(30%, Merck - Darmstadt, Germany).
c) Preparation of the oil samples to analysis by Rancimat: Samples of 3.0 ±0.1 g of oils with
volatile oxidation products were stripped from the oil and dissolved in deionized water.
d) Analysis by UV/VIS: Samples of the Dersani
®
and Sunflower
®
oils were diluted in HPLC
grade hexane at a concentration of 10 g/L.
e) Analysis by Fluorescence: The samples were diluted in HPLC grade hexane at a con-
centration of 10 g/L.
Experimental features The gas chromatography method allowed the determination of 14 fatty acids.
Determination of concentration levels of contents (Al, Ca, Cr, Fe, K, Mg, Mn, Na, P, Se and Zn)
in Dersani
®
and sunflower
®
oils used in the treatment of wounds.
Induction times were determined on each sample in a Metrohm Rancimat.
Identification of phenolic compounds and tocopherols in Dersani
®
and Sunflower
®
oils using
UV-VIS spectroscopy.
Identification of molecular groups in Dersani
®
(Tocopherols, tocotrienols) and Sunflower
®
(alpha-tocopherol and methyl-linolenate) oils using fluorescence spectroscopy.
Data source location City of Campo Grande, State of Mato Grosso do Sul, Brazil.
Data accessibility The raw data was attached to this data article as Supplementary Material.
Value of the data
Data on the oxidative stability of essential fatty acid oil can be compared with the international standard EN14112 for
samples of sunflower and vegetable oil, as well as others oils used in the treatment of wounds.
Data on the presence or absence of the level of metals such as Cu, Fe, Mn and Ni are involved in the oxidation of oils.
Studies should be performed considering other types of oils used in the treatment of wounds.
The datasets obtained from different techniques, lead to different results respectively for Dersani
®
oil and Sunflower
®
oil.
The study would a lead to new questions and investigations of oils used in the treatment of wounds.
This data are important for monitoring purposes and also to fill the gaps in two oils used to treatment of wounds.
J.B. de Oliveira et al. / Data in brief 26 (2019) 1044272
1. Data
Dersani
®
oil is used to treat wounds and is composed of essential fatty acids [1]. On the other hand,
cold-pressed sunflower seed oil (Helianthus annus) has been used topically to improve the skin barrier
and prevent systemic infections [2].
The manuscript is organized as follow: the data presented in subsection 1.1 (Table 1) include results
on fatty acids composition of oils by CG analysis. In subsection 1.2 (Table 2) we presented data on
elemental content in Dersani
®
and Sunflower
®
oils detected by ICP OES. The subsection 1.3 (Table 3,
Table 1
Fatty acids composition of Dersani
®
and Sunflower
®
oils.
Fatty acids (%) Dersani
®
Sunflower
®
Capryc (C10:0) 0.12 0.12
Lauric (C12:0) 0.11 0.11
Myristic (C14:0) 0.18 0.17
Palmitic (C16:0) 6.89 7.02
Palmitoleic (C16:1) 0.32 0.3
Stearic (C18:0) 2.67 2.74
Oleic (C18:1) 32.18 32.44
Linoleic (C18:2) 54.81 54.41
Linolenic (C18:3) 0.32 0.31
Arachidic (C20:0) 0.24 0.26
Gondoic (C20:1) 0.46 0.45
Behenic (C22:0) 0.98 0.95
Erucic (C22:1) 0.44 0.45
Lignoceric (C24:0) 0.28 0.28
SFA 11.47 11.65
MUFA 33.4 33.65
PUFA 54.81 54.72
Table 2
Analytical data on elemental content present in the Dersani
®
and Sunflower
®
oils detected in ICP OES (in units of mg/
Kg ±standard deviation of triplicate).
Elements Dersani
®
Oil (mg/Kg) Sunflower
®
Oil (mg/Kg)
Al 10.30 ±0.03 118.20 ±0.40
Ca 41.00 ±0.60 28.25 ±0.04
Co <LOQ <LOQ
Cr 3.60 ±0.11 3.45 ±0.07
Cu <LOQ <LOQ
Fe 1.30 ±0.02 1.40 ±0.04
K 11.00 ±0.12 46.50 ±0.05
Mg 22.50 ±0.14 15.04 ±0.03
Mn 1.30 ±0.02 <LOQ
Na 243.50 ±27.80 58.20 ±0.10
Ni <LOQ <LOQ
P 127.50 ±4.40 169.50 ±1.23
S<LOQ <LOQ
Se 0.90 ±0.08 2.00 ±0.01
Zn 2.35 ±0.08 1.60 ±0.01
<LOQ - Analyte concentrations were below the limits of detection.
Table 3
Rancimat at 110
C of the Dersani
®
and Sunflower
®
oils.
Dersani
®
oil Sunflower
®
oil
Racimat [h] 1.56 ±0.02 6.21 ±0.17
Mean ±SD: Standard deviation values are expressed as mean of samples analyzes in triplicates.
J.B. de Oliveira et al. / Data in brief 26 (2019) 104427 3
Fig. 1) provides data on oxidative stability obtained by Rancimat method. In the subsection 1.4 (Fig. 2),
data on UV-VIS are presented as a graph of absorbance versus wavelength. The subsection 1.5 (Figs. 3
and 4) includes the data on excitation and emission map for fluorescence of the Dersani
®
and sun-
flower
®
oils.
1.1. Data obtained by analysis by CG
Data on chromatographic analysis used to estimate the percentages of saturated fatty acids (SAT),
monounsaturated fatty acids (MUFA) and polyunsaturated fatty acids (PUFA) of the oils examined in
this article are in Table 1.
1.2. Data analysis by ICP OES
In Table 2, a total of 11 elements (Al, Ca, Cr, Fe, K, Mg, Mn, Na, P, Se and Zn) were quantified in the
Dersani
®
and Sunflower
®
oils. The data in Table 2 show that Co, Cu, Ni and S level was below limit of
Fig. 1. Conductivity versus time determined by the Rancimat method. Oxidation stability of oils at 110 C. The induction time of
Dersani
®
and Sunflower
®
oils were 1.56 and 6.21 hours.
Fig. 2. UV-vis absorption spectra of absorbance versus wavelength from 200e750 nm for the Dersani
®
and Sunflower
®
oils.
J.B. de Oliveira et al. / Data in brief 26 (2019) 1044274
detection in the samples. The experiments were analyzed by ICP OES after digestion procedures (Tables
4 and 5).
1.3. Data on thermal stability obtained from Rancimat
The oxidative stability index of oils (induction period time) was given in Table 3. The Rancimat
induction time at 110
C varied from 1.56 ±0.02 to Dersani
®
oil and 6.21 ±0.17 h to Girassol
®
oil (Table
Fig. 3. Excitationeemission map of Dersani
®
oil obtained by exciting between 225 and 450 nm and emission in the 250e700nm
range.
Fig. 4. Excitationeemission map of Sunflower
®
oil obtained by exciting between 225 and 450nm and emission in the 250e70 0 nm
range.
Table 4
Operating program for the microwave digestion system.
Step Power (W) Temperature (
C) Hold Time (min) Ramp time (min) Pressure (Bar)
1 1305 150 10 5 35
2 1305 250 15 3 35
3 0 50 10 1 35
4 0 50 0 0 35
5 0 50 0 0 35
J.B. de Oliveira et al. / Data in brief 26 (2019) 104427 5
3). The Fig. 1 shows the example of an analysis the induction time of 1.56 h to Dersani oil and 6.21 h to
sunflower oil.
1.4. Data on UV-VIS
Fig. 2 shows typical plots of UV-Vis absorption spectra of the two oils from 200 to 750 nm. All oils
have two absorption peaks from 250 to 350 nm. Dersani
®
oil shows a band centered near 330 nm
and low intensity bands between 260 and 300 nm. Sunflower oil showed bands already known at
approximately 232 and 270 nm, which are used for monitoring the degradation products in vege-
table oils. In this selected wavelength range the absorption of phenolic compounds (270e330 nm)
and tocopherols (325 nm) occurs, although the contribution of some fatty acids should not be
excluded.
1.5. Data on fluorescence
The Figs. 3 and 4 shows the excitation-emission map of Dersani
®
and Sunflower
®
oils obtained by
exciting at 225e450 nm and collecting the emission in the range 250e700 nm. The oils show differ-
ences in their fluorescence spectra.
The emission of Dersani
®
oil occurs in the region near 500 nm for excitation wavelengths at 370
nm. Such emission can be associated with the presentation of vitamin E, a group of natural antioxi-
dants composed of tocopherols and tocotrienols. On the other hand, the emission bands of Sun-
flower
®
oil may be derived from the presence of alpha-tocopherol which when excited near 300 nm
should emit at 325 nm, and methyl-linolenate with emission around 400 nm when excited at 300 and
320 nm.
2. Experimental design, materials and methods
2.1. Material
Samples of the two most popular brands of essential oils were purchased at local pharmacies in the
city of Campo Grande, Brazil. Total ten samples of one brand of each sample were taken for study. In
this way total 20 samples (10 samples for Dersani [Manufacturer: Laborat
orio Daudt Oliveira Ltda] and
10 sunflower oil [Manufacturer: Phytotratha] respectively) were collected for study considering the
same batch reference number and date of manufacture.
Table 5
Analytical characteristics of ICPeOES method: Limit of detection (LODs), limit of quantification (LOQs) and correlation coefficient
(R
2
).
Elements LOD (mg L
1
) LOQ (mg L
1
)R
2
Al 0.003 0.010 0.999
Ca 0.02 0.08 0.999
Co 0.0002 0.0008 0.999
Cr 0.0004 0.001 0.999
Cu 0.002 0.006 0.999
Fe 0.0009 0.003 0.999
K 0.01 0.04 0.999
Mg 0.01 0.03 0.999
Mn 0.0001 0.0004 0.999
Na 0.0001 0.0005 0.999
Ni 0.002 0.005 0.999
P 0.005 0.02 0.999
Se 0.001 0.005 0.999
Zn 0.0006 0.002 0.999
J.B. de Oliveira et al. / Data in brief 26 (2019) 1044276
2.2. Chromatographic method
The sample preparation procedure was performed according to Ref. [3] as follow: a) an amount of
0.16 g of Dersani oil and 0.16 g of sunflower oil was weighed separately, and for the esterification
process 4 ml of KOH (5%) in MeOH was added to each sample; b) samples were placed in a thermal bath
with boiling water for 5 minutes; c) after cooling, 5 mL of NH
4
CleH
2
SO
4
eMeOH (0.5:10:89.5 w:v:v)
was added in each oil sample, which were homogenized on a vortex mixer (Labnet International S0200
VX-200 Vortex Mixer with Combi head) for 30 seconds and placed in boiling water for 5 minutes; d)
after cooling, each sample was individually homogenized after the addition of 4 ml of saturated NaCl
solution; e) subsequently, 5 ml of hexane was added to the oil samples and then homogenized again
using a vortex mixer; f) a quantity of 1
m
L of each sample was used by the chromatography equipment.
The analysis of fatty acid composition by gas chromatography was performed on a Thermo Fisher
Scientific (FOCUS GC) chromatograph equipped with a flame ionization detector (FID) and manual
injector, capillary column DB-Wax (30 m length, 0.32 mm internal diameter, and 0.25
m
moffilm, J &W
Scientific). The injector temperature was programmed at 250
C; column temperature at 180
C for 20
min, and ramp rate of 2
C/min up to 220
C; temperature of the detector at 260
C. Hydrogen gas was
used as the mobile phase at a rate of 1.0 mL/min. “Make up nitrogen”gas flow at a flow of 20 mL/min
was used to minimize band broadening and injection volume of 1
m
L. For the identification of fatty
acids, the retention times were compared with those of the methyl ester standards (Sigma-Aldrich),
while the quantification was performed by area normalization, expressing the result in percentage of
area of each acid over the total area of fatty acids (%).
2.3. Microwave-assisted digestion
A microwave system (Speedwave four
®
, Berghof, Germany) was used for digestion of samples.
About 0.7 g of each samples were weighted, transferred inside Teflon vessels and 6 ml of HNO
3
(65%,
Merck - Darmstadt, Germany) and 2 ml of H
2
O
2
(30%, Merck - Darmstadt, Germany) were added. All the
samples were digested in triplicates. The microwave instrumental parameters and conditions used for
oils are reported in Table 4, respectively. A blank solutions were prepared using 6 ml deionized water
(18 M
U
cm) obtained from a Milli-Q Plus system (Millipore, Bedford, MA, USA), with 6 ml of
concentrated HNO
3
(65%, Merck - Darmstadt, Germany) and 2 ml of concentrated H
2
O
2
(30%, Merck -
Darmstadt, Germany).
2.4. Process of data analysis by ICP OES
Analysis were carried out using an inductively coupled plasma optical emission spectrometry
model iCAP 6300 (Thermo Fisher Scientific, Bremen, Germany) with axial configuration. The operating
conditions of the ICP-OES equipment were 1250 Wof a RF power,12 L.min
1
of a plasma flow rate, 0.5
L.min
1
of an auxiliary gas flow rate, 0.45 L.min
1
of a nebulizer flow rate and 20 s of stabilization time.
The analytical emission lines (nm) were determined by the operational software iTEVA of ICP OES
instrument as follows: Al (396.100 nm); Ca (422.673 nm); Co (228.616 nm); Cr (267.716 nm); Cu
(324.754 nm); Fe (259.940 nm); K (766.490 nm); Mg (279.553 nm); Mn (257.610 nm); Na (588.995
nm); Ni (221.647 nm); P (214.914 nm); Cu (327.396 nm); Se (196.09 nm) and Zn (213.856 nm). High
purity argon 99,996% (White-Martins-Praxair, MS, Brazil) was used to purge the optics and to form the
plasma.
A multi-element stock solutions (Specsol, S~
ao Paulo, Brazil) containing 100 mg.L
1
of each elements
(Al, Ca, Co, Cr, Cu, Fe, K, Mg, Mn, Na, Ni, P, Se and Zn) was used to prepare the calibration solution by
sequential solution with ultrapure water (18 M
U
cm). To determine the concentration of elements in
both oils, calibration curves were built on seven different concentrations. All determinations were done
in triplicate. A blank was carried out in the same way. From the resources of the ITEVA software that
controls ICP OES, we checked the small region of the spectrum to determine if a line for a given element
is free of interference from lines of other elements.
The detection limit (LODs) and quantification limit (LOQs) was calculated according to IUPAC [4].
They are calculated as follows: LOD ¼3*stdError/A1; LOQ ¼10*stdError/A1, where stdError ¼the
J.B. de Oliveira et al. / Data in brief 26 (2019) 104427 7
standard error of estimation of the background signal and A1 ¼gain coefficient of the calibration curve.
R
2
is the square of the correlation coefficient. A perfect line would have an R
2
value of 1, and most R
2
values for calibration curves are over 0.999 for all analytes (see Table 5).
2.5. Process of data analysis by Rancimat
The oxidative stability of the oils was evaluated by mean of the Rancimat method. Stability was
expressed as the oxidative induction period (IP, hrs) measured at 110
C on a Rancimat 893 (Metrohm
Co, Basel) and air flow of 10 L/hr. Samples of 3.0 ±0.1 g of oil with a Volatile oxidation products were
stripped from the oil and dissolved in deionized water. The increased of conductivity was expressed
through a curve from which the inductionperiod can be calculated by the interception of tangent to the
inclination and the tangent to the curve level part. Thus, the time taken to reach a level of conductivity
was measured (see Fig. 1). The methodology adopted was performed according to the European
standard EN14112 and according to the guidance of the National Agency of Petroleum [5], Natural Gas
and Biofuels (ANP) [6]. For both oils, analyzes were performed in triplicate.
2.6. Process of data analysis by UV/VIS
Samples of the Dersani
®
and Sunflower
®
oils were diluted separately in HPLC grade hexane at a
concentration of 10 g/L. UV-visible absorption measurements were performed using a spectropho-
tometer (Lambda 265UV/Vis, PerkinElmer, Waltham, MA, USA) and an optical cuvette made from
quartz with a 10 mm optical path and with four polished faces was used as a sample holder. The UV-
visible absorption spectra were collected in the 200e750 nm range.
2.7. Process of data analysis by fluorescence
Excitation-emission matrix fluorescence spectra were measured using a spectrofluorometer (FS-2,
Scinco, Seoul, Korea). The excitationeemission matrices of fluorescence were obtained by exciting the
samples in the wavelengths from225 to 450 nm in 5 nm steps and collecting the emission between 250
and 700 nm in 1 nm steps. The excitation and emission slits were 5 nm and the sensitivity of the
detector was 600 V. The samples were diluted in HPLC grade hexane at a concentration of 10 g/L. A
four-sided quartz cell with 10 mm optical path was used. A four-sided quartz cell with 10 mm optical
path was used.
Acknowledgements
We would like to thank National Council for Scientific and Technological Development (CNPq),
Brazil, for the financial support (CNPq: Process No 311336/2017-5). This study was financed in part by
the Coordenaç~
ao de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001.
Conflict of interest
The authors declare that they have no known competing financial interests or personal relation-
ships that could have appeared to influence the work reported in this paper.
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