No full-text available
To read the full-text of this research,
you can request a copy directly from the authors.
Chapter 11. Determination of the Identity, Content and Purity of Therapeutic Peptides by NMR Spectroscopy
Abstract
This chapter describes the application of NMR spectroscopy to the determination of the identity, content, structure and purity of peptides. Both 1D and 2D NMR examples are presented for both ¹H and other nuclei. NMR spectroscopy is a nondestructive technique requiring limited sample pretreatment and development time. The limited sample pretreatment reduces the experimental time and variations due to handling, thereby increasing robustness. NMR spectroscopy can distinguish between very closely related peptides. It is especially suited for the determination of unrelated impurities such as process-related impurities and extractables/leachables. NMR spectroscopy is very sensitive to higher-order structure. Although NMR equipment in itself is expensive, the actual cost of an NMR spectrum is low owing to automation and limited consumables.
ResearchGate has not been able to resolve any citations for this publication.
- B Valentine
- A Steinschneider
- D Dhawan
- M I Burgar
- T S T Amour
- D Fiat
B. Valentine, A. Steinschneider, D. Dhawan, M. I. Burgar, T. S. T. Amour
and D. Fiat, Int. J. Pept. Protein Res., 1985, 25, 56.
- R Hoffmann
- I Reichert
- W O Wachs
- M Zeppezauer
- H R Kalbitzer
R. Hoffmann, I. Reichert, W. O. Wachs, M. Zeppezauer and
H. R. Kalbitzer, Int. J. Pept. Protein Res., 1994, 44, 193.
- J T Gerig
J. T. Gerig, Prog. Nucl. Magn. Reson. Spectrosc., 1994, 26, 293.
- P Mykhailiuk
- S Afonin
- G V Palamarchuk
- O V Shishkin
- A S Ulrich
- I V Komarov
P. Mykhailiuk, S. Afonin, G. V. Palamarchuk, O. V. Shishkin, A. S. Ulrich
and I. V. Komarov, Angew. Chem., Int. Ed. Engl., 2008, 47, 5765-5767.
- S Afonin
- R W Glaser
- M Berditchevskaia
- P Wadhwani
- K.-H Gührs
- U Möllmann
- A Perner
- A S Ulrich
S. Afonin, R. W. Glaser, M. Berditchevskaia, P. Wadhwani, K.-H. Gührs,
U. Möllmann, A. Perner and A. S. Ulrich, ChemBioChem, 2003, 4, 1151-1163.
- E Kellenbach
E. Kellenbach, et al., NMR Spectroscopy in Pharmaceutical Analysis, ed.
U. Holzgrabe, I. Wawer and B. Diehl, Elsevier, Amsterdam, 1st edn, 2008,
ch. 5, pp. 429-436;
- E Kellenbach
E. Kellenbach, et al., Pharmeuropa, 2008, 2008, 1-8.
- A Bundi
- K Wüthrich
A. Bundi and K. Wüthrich, Biopolymers, 1979, 18, 285.
- D S Wishart
- B D Sykes
D. S. Wishart and B. D. Sykes, Methods Enzymol., 1994, 239, 363.
- G Maniara
- K Rajamoorthi
- S Rajan
- G W Stockon
G. Maniara, K. Rajamoorthi, S. Rajan and G. W. Stockon, Anal. Chem.,
1998, 70, 4921.
- F Malz
- H Jancke
F. Malz and H. Jancke, J. Pharm. Biomed. Anal., 2005, 38, 813.
- Y B Monakhova
- B W K Diehl
Y. B. Monakhova and B. W. K. Diehl, J. Pharm. Biomed. Anal., 2018,
154, 332.
- A Preiß
- J Kruppa
- J Buschmann
- C Mügge
A. Preiß, J. Kruppa, J. Buschmann and C. Mügge, J. Pharm. Biomed. Anal.,
1998, 16, 1381.
- C W Funke
- J.-R Mellema
- P Salemink
- G N Wagenaars
C. W. Funke, J.-R. Mellema, P. Salemink and G. N. Wagenaars, J. Pharm.
Pharmacol., 1988, 40, 78.
- J A Hoffmann
- R E Chance
- M G Johnson
J. A. Hoffmann, R. E. Chance and M. G. Johnson, Protein Expression
Purif., 1990, 1, 127.
- A C Gucinski
- M T Boyne
- D A Keire
A. C. Gucinski, M. T. Boyne II and D. A. Keire, Anal. Bioanal. Chem., 2015,
407, 749.
- C K Larive
- D Jayawickrama
- L Orfi
C. K. Larive, D. Jayawickrama and L. Orfi, Appl. Spectrosc., 1997,
51, 1531.
- D J Craik
- K A Higgins
D. J. Craik and K. A. Higgins, Pept. Res., 1991, 4, 177.
- A D Kline
- R M Justice
A. D. Kline and R. M. Justice Jr., Biochemistry, 1990, 29, 2906.
- C Grathwohl
- K Wüthrich
C. Grathwohl and K. Wüthrich, Biopolymers, 1976, 15, 2025.
- K Hu
- W M Westler
- J L Markley
K. Hu, W. M. Westler and J. L. Markley, J. Am. Chem. Soc., 2011, 133,
1662-1665.
- C Li
C. Li, et al., J. Pharm. Biomed. Anal., 2019, 166, 105.
- K Skidmore
- D Hewitt
- Y.-H Kao
K. Skidmore, D. Hewitt and Y.-H. Kao, Biotechnol. Prog., 2012, 28, 1526.
- N Magarian
- K Lee
- K Nagpal
- K Skidmore
- E Mahajan
N. Magarian, K. Lee, K. Nagpal, K. Skidmore and E. Mahajan, Biotechnol.
Prog., 2016, 32, 718.
- Y.-H Kao
- J Bender
- A Hagewiesche
- P Wong
- Y Huang
- M Vanderlaan
Y.-H. Kao, J. Bender, A. Hagewiesche, P. Wong, Y. Huang and
M. Vanderlaan, PDA J. Pharm. Sci. Technol., 2001, 55, 268.
- E Kellenbach
- M Burgering
- F Kaspersen
E. Kellenbach, M. Burgering and F. Kaspersen, Org. Process Res. Dev.,
1999, 3, 141.
- M Buck
M. Buck, Q. Rev. Biophys., 1998, 31, 297.
- A Jasanoff
- A R Fersht
A. Jasanoff and A. R. Fersht, Biochemistry, 1994, 33, 2129.
- A Motta
- D Picone
- T Tancredi
- P A Temussi
A. Motta, D. Picone, T. Tancredi and P. A. Temussi, Tetrahedron, 1988,
44, 975.
- R Spadaccini
- O Crescenzi
- D Picone
- T Tancredi
- P A Temussi
R. Spadaccini, O. Crescenzi, D. Picone, T. Tancredi and P. A. Temussi,
J. Pept. Sci., 1999, 5, 306.
- D Picone
- A Ursi
- A Motta
- T Tancredi
- P A Temussi
D. Picone, A. D'Ursi, A. Motta, T. Tancredi and P. A. Temussi, Eur. J.
Biochem., 1990, 192, 433.
- D A Kallick
- M R Tessmer
- C R Watts
- C Y Li
D. A. Kallick, M. R. Tessmer, C. R. Watts and C. Y. Li, J. Magn. Reson., Ser.
B, 1995, 109, 60-65.
- F Åslund
- K Nordstrand
- K D Berndt
- M Nikkola
- T Bergman
- H Ponstingl
- H Jörnvall
- G Otting
- A Holmgren
F. Åslund, K. Nordstrand, K. D. Berndt, M. Nikkola, T. Bergman,
H. Ponstingl, H. Jörnvall, G. Otting and A. Holmgren, J. Biol. Chem.,
1996, 271, 6736.
- D Wishart
D. Wishart, Curr. Pharm. Biotechnol., 2005, 6, 105.
- S L Mansfield
- D A Jayawickrama
- J S Timmons
- C K Larive
S. L. Mansfield, D. A. Jayawickrama, J. S. Timmons and C. K. Larive,
Biochim. Biophys. Acta, Protein Struct. Mol. Enzymol., 1998, 1382, 257.
- S L Mansfield
- A J Gotch
- G S Harms
- C K Johnson
- C K Larive
S. L. Mansfield, A. J. Gotch, G. S. Harms, C. K. Johnson and C. K. Larive,
J. Phys. Chem. B, 1999, 103, 2262.
- D I Freedberg
- Y Aubin
- C Jones
D. I. Freedberg, Y. Aubin and C. Jones, BioPharm Int, 2010, 6(Supplement
August), 28.
- M Lindberg
- J Jarvet
- Ü Langel
- A Gräslund
M. Lindberg, J. Jarvet, Ü. Langel and A. Gräslund, Biochemistry, 2001,
40, 3141.