ArticlePDF Available
230 Crop Breeding and Applied Biotechnology - 19: 230-234, 2019
MS Carneiro et al.
RB005014 – a sugarcane culvar with high
llering and agroindustrial yield
Monalisa Sampaio Carneiro1*, Roberto Giacomini Chapola1,
Antonio Ribeiro Fernandes Junior2, Danilo Eduardo Cursi1,
Thiago Willian Almeida Balsalobre1 and Hermann Paulo
Homann1
Abstract: RB005014 was developed for the Brazilian central-south region, for
harvesng between July and September and planng on soils that have mod-
erate or higher ferlity levels. It has high llering, high sucrose yield, excellent
ratooning ability aer mechanical harvesng, resistance to the main diseases
and carries the Bru1 gene of brown rust resistance.
Keywords: Saccharum spp., improvement, disease resistance.
Crop Breeding and Applied Biotechnology
19: 230-234, 2019
Brazilian Society of Plant Breeding.
Printed in Brazil
hp://dx.doi.org/10.1590/1984-
70332019v19n2c32
CULTIVAR RELEASE
*Corresponding author:
E-mail: monalisa@ufscar.br
ORCID: 0000-0002-9835-7205.
Received: 26 February 2018
Accepted: 14 February 2019
1 Universidade Federal de São Carlos, Depar-
tamento de Biotecnologia e Produção Vegetal
e Animal, 13.600-970, São Paulo, SP, Brazil
2 Universidade Federal de São Carlos, Estação
Experimental de Valparaíso, 16.880-000,
Valparaíso, SP, Brazil
INTRODUCTION
Modern sugarcane culvars have a complex genome, due to large genome
size arround 10 Gb, a variable ploidy level and constant aneuploidy resulng
in highly heterozygous hybrids (Vieira et al. 2018). Culvated sugarcane is
a vegetavely propagated crop, which takes approximately 8 to 12 years to
improve and release more producve culvars.
The Sugarcane Breeding Program of the Federal University of São Carlos
– PMGCA/UFSCar (www.ridesaufscar.com.br) is part of the Inter-University
Network for the Development of Sugarcane Industry – RIDESA (www.ridesa.
com.br). In over 40 years, 94 RB sugarcane culvars were released, currently
planted on nearly 64% of the sugarcane growing area in Brazil (Daros et al.
2015, Chapola et al. 2016).
The RIDESA network aims to develop high-yielding culvars with a high
sucrose content, resistance to the main diseases and adapted to dierent climate
and soil condions (Carneiro et al. 2016, Daros et al. 2018). Furthermore, the
stability of culvar yields in mechanical planng and harvesng systems has been
a challenge and concern for RIDESA (Daros et al. 2018). In this context, the high
llering and excellent ratooning ability of RB005014, even under mechanical
harvesng, indicate the culvar as promising. Moreover, this sugarcane culvar
is suitable for mechanized planng and has good resistance levels against the
major sugarcane diseases.
PEDIGREE AND BREEDING METHOD
Culvar RB005014 was derived from a biparental cross of the full-sib
genotypes SP80-1816 x RB855536 (Figure 1). At the beginning of the year
RB005014 – a sugarcane culvar with high llering and agroindustrial yield
231Crop Breeding and Applied Biotechnology - 19: 230-234, 2019
2000, the cross was carried out at the experimental site
Estação de Floração e Cruzamento da Serra do Ouro (lat 9º
13’ S, long 35º 50’ W, alt 450 m asl), in the municipality of
Murici, Alagoas, of the Federal University of Alagoas. Later
in the same year, sugarcane caryopses were planted and
germinated in a greenhouse at the experimental staon
of the Federal University of São Carlos (lat 22° 21’ S, long
47° 23’ W, alt 620 m asl), in the city of Araras, São Paulo.
Thereaer the sugarcane plantlets were individualized and
planted at an experimental eld in order to establish the
rst selecon stage (T1). In this phase, each genotype,
represented by a single clump, was mass-selected in the
rst ratoon crop for general morphological criteria like
as higher Brix, resistance to the main diseases, absence
of owering, stalk number and reduced bagasse pith
(Morais et al. 2015).
The clones selected in the T1 stage were taken to the second selecon stage (T2), which also had standard
commercial culvars for comparison of yields. In this phase, the genotypes were grown at two locaons in the state
of São Paulo: Araras (lat 22° 21’ S, long 47° 23’ W, alt 620 m asl) and Valparaiso (lat 21° 13’ S, long 50° 52’ W, alt 439
m asl). The experiment in T2 stage was evaluated in an augmented randomized incomplete block design (Federer
1956), with plots consisng of two 2.5-m rows, with one replicaon. Genotypes in T2 stage were assessed in plant
cane and ratoon crops likewise in T1 stage, plus the variables stalk weight per plot (WP) and kilogram brix per plot
(KBP) (Kang et al. 1983). Clones selected during T2 stage advanced for the third selecon stage (T3) as described
by Carneiro et al., (2016). In T3, clones were evaluated at three sites under dierent climate and soil condions,
in dierent regions of Sao Paulo state (Tarumã-SP (lat 22° 44’ S, long 50° 34’ W, alt 429 m asl), Nova Europa-SP (lat
21° 46’ S, long 48° 33’ W, alt 502 m asl), Barra Bonita-SP (lat 22° 28’ S, long 48° 33’ W, alt 526 m asl). The selecon
in T3 stage was performed considering the performance of the clones across all evaluated environments and both
plant and ratoon crops. The selecon criteria were ulized sucrose content in sugarcane (PC, in %) and kilogram pol
per plot (KPP).
The selected genotypes were planted in the experimentaon stage (ES), in which they were assessed in 15 elds
trials allocated in the diverse in regions of São Paulo and Mato Grosso do Sul: Tarumã-SP (lat 22° 44’ S, long 50° 34’
W, alt 429 m asl), Nova Europa-SP (lat 21° 46’ S, long 48° 33’ W, alt 502 m asl), Barra Bonita-SP (lat 22° 28’ S, long
48° 33’ W, alt 526 m asl), Guaíra-SP (lat 20° 19’ S, long 48° 18’ W, alt 518 asl), Pradópolis-SP (lat 21° 21’ S, long 48°
4’ W, alt 533 asl), Promissão-SP (lat 21° 32’ S, long 49° 51’ W, alt 425 asl), Valparaíso-SP (lat 21°13’ S, long 50° 52’
W, alt 439 m asl), Olímpia-SP (lat 20° 44’ S, 48° 54’ W, alt 480 asl), Tanabi-SP (lat 20° 37’ S, long 49° 39’ W, alt 521 m
asl), Paraguaçu Paulista-SP (lat 22° 24’ S, long 50° 34’ W, alt 509 m asl), Piracicaba-SP (lat 22° 43’ S, long 47° 38’ W,
alt 526 m asl), Orindiúva-SP (lat 20° 11’ S, long 49° 21’ W, alt 487 m asl), Guariba-SP (lat 21° 21’ S, long 48° 13’ W, alt
649 m asl), Sandovalina-SP (lat 22° 27’ S, 51° 45’ W, alt 383 m asl), Angélica-MS (lat 22° 9’ S, long 53° 46’ W, alt 366
m asl)), recording the data of three to four cycles. The elds tests were established in the randomized block (3 or 4
replicates), with standard commercial culvars as controls, alocated in the blocks. The traits assessed were sucrose
content in sugarcane (PC, in %), tons of stalks per hectare (TSH), tons of pol per hectare (TPH), and ber content
(in, %). The clone adaptability and stability were esmated according by Eberhart and Russell (1966). The selected
genotypes of ES were evaluated to maturaon curve, according to the sucrose content in sugarcane (PC, in %).
PERFORMANCE
Culvar RB005014 has an intermediate development cycle and upright growth habit. The stalks have a medium
diameter, a high amount of wax, a greyish green color and the leaf blades are green and waxy. The llering capacity in
plant and ratoon crops is high, with excellent canopy cover, high ratooning ability even under mechanical harvesng,
and sugarcane longevity (several harvests from one planng). In addion, culvar RB005014 has a high agro-
Figure 1. Pedigree of sugarcane culvar RB005014.
232 Crop Breeding and Applied Biotechnology - 19: 230-234, 2019
MS Carneiro et al.
industrial yield, yield stability and good ber content.
Under the condions of central-south region of Brazil, the
recommended harvest me for RB005014 is the middle
of the growing season, between July and September
(Figure 2). Under these condions, culvar RB005014
rarely owers and produces lile or no pith.
Culvar RB005014 had responsiveness to improvements
soil and climac condions. Considering the standard
commercial culvar (RB867515), the culvar RB005014
had greater agricultural yield (TSH) in the intermediate
to favorable environments, whereas in restricted
environments presented TSH lower yields than standard
commercial (Figure 3). The RB005014 was evaluated in pre-
commercial areas, and RB005014 culvar recommendaon
is for favorable and intermediate soil and climatic
condions, according to the classicaon of Prado (2008).
Culvar RB005014 produced an agricultural yield (TSH)
of more than 119 T ha-1 and a cane sucrose content (PC,
in %) of approximately 15.5%. The performance of this
culvar with regard to the agroindustrial yield (in tons
of pol per hectare - TPH) was excellent, higher to that
of commercial standard culvars of intermediate/late
maturaon, considering the mean data of 13 eld tests
with three to four harvests each (Figure 4).
OTHER CHARACTERISTICS
Disease reacon
Culvar RB005014 was subjected to tests of arcial
inoculaon and natural infecon with the main sugarcane
diseases, together with other genotypes. These tests
assessed the reaction of clones and cultivars against
these diseases under the conditions of central-south
region of Brazil.
Disease evaluaon under natural infecon condions
were performed in areas with weather conditions to
pathogen occurrence and, consequently, with high inoculum pressure. Thereby, we evaluated under natural infecon
condions the main sugarcane diseases; orange rust (Puccinia kuehnii), brown rust (Puccinia melanocephala), smut
(Sporisorium scitamineum), leaf mosaic (sugarcane mosaic virus) and leaf scald (Xanthomonas albilineans). The
evaluaon is based on the number of infected clumps (% incidence) for smut, mosaic and scald, and based on the
leaf area percentage with symptoms (% severity) for orange and brown rusts (Amorim et al. 1987).
Greenhouse tests of arcial inoculaon with smut fungus spores and contaminaon with mosaic virus suspension
were carried out, as described by Matsuoka (1979). The evaluaon was followed by scale for each disease, which
considers the amount of infected plants (% incidence) and the clones were categorized as resistant, intermediate
or suscepble. The results in both tests indicated RB005014 as highly resistant to the diseases evaluated (Table 1);
therefore, it is recommended for planng without restricon.
The brown rust resistance in modern sugarcane culvars is largely due to the Bru1 gene presence. Thereby, to verify
if culvar RB005014 has this resitance gene, the genomic DNA was extracted as described by Aljanabi et al. (1999)
Figure 2. Maturaon curve of sugarcane culvar RB005014 in
comparison with the commercial standard culvars RB867515
and RB92579.
Figure 3. Adaptability and stability of culvar RB005014 in com-
parison with the commercial standard culvar RB867515. The
mean data of tons of stalks per hectare (TSH) were adjusted based
on regression analysis (Eberhart and Russell 1966). The points
indicate the dataset of 13 experiments, with four harvests each.
RB005014 – a sugarcane culvar with high llering and agroindustrial yield
233Crop Breeding and Applied Biotechnology - 19: 230-234, 2019
and aer that the presence of the Bru1 gene was detected
using the molecular markers R12H16 and 9O20-F4-RsaI
(Costet et al. 2012). The PCR reacons and amplicaon
condions were carried out as proposed by Costet et al.
(2012). The result showed that Bru1 gene was present
in culvar RB005014, aested by the posive diagnosis
of the two molecular markers evaluated (haplotype 1).
BASIC SEED MAINTENANCE AND
DISTRIBUTION
The samples of culvar RB005014 are keepng and
distributed by the Sugarcane Breeding Program (hps://
www.ridesaufscar.com.br/) of the Department of Biotechnology, Plant and Animal Producon, Center of Agricultural
Sciences, Federal University of São Carlos, Araras, São Paulo, Brazil.
Figure 4. Isoquants of mean tons of pol per hectare (TPH) in funcon of sucrose content (PC in %), in sugarcane and tons of stalks
per hectare (TSH) in 13 experiments. In the black circle, culvar RB005014 is compared with standard commercial culvars (gray
circles) and clones (void circles).
Table 1. Reacon of sugarcane culvar RB005014 to diseases in
the central-south region of Brazil
Disease Culvar RB005014
Smut R
Brown rust R+
Orange rust R
Mosaic R
Leaf Scald R
R = resistant; + = Presence of molecular markers Bru1 (haplotype 1: presence of the
two markers R12H16 and 9O20-F4-RsaI)
REFERENCES
Aljanabi S, Forget L and Dookun A (1999) An improved and rapid protocol
for the isolaon of polysaccharide-and polyphenol-free sugarcane
DNA. Plant Molecular Biology Reporter 17: 281-281.
Amorim L, Bergamin-lho A, Sanguino A, Cardoso C, Moraes VA and
Fernandes CR (1987) Metodologia de avaliação da ferrugem da cana-
de-açúcar (Puccinia melanocephala). Bolem Técnico COPERSUCAR
39: 13-16.
Carneiro MS, Chapola RG, Fernandes Júnior AR, Cursi DE, Barreto FZ,
Balsalobre TWA and Homann HP (2016) RB975242 and RB975201
- Late maturaon sugarcane variees. Crop Breeding and Applied
Biotechnology 16: 365-370.
Chapola RG, Fernandes Júnior AR, Cursi DE and Homann HP (2016)
Censo de variedades de cana-de-açúcar nos estados de São Paulo e
Mato Grosso do Sul em 2015. STAB 34: 40-42.
Costet L, Le Cun L, Royaert S, Raboin LM, Hervouet C, Toubi L, Telismart
H, Garsmeur O, Rousselle Y, Pauquet J, Nibouche S, Glaszmann JC,
234 Crop Breeding and Applied Biotechnology - 19: 230-234, 2019
MS Carneiro et al.
This is an Open Access arcle distributed under the terms of the Creave Commons Aribuon License, which permits unrestricted
use, distribuon, and reproducon in any medium, provided the original work is properly cited.
Hoarau JY and D’Hont A (2012) Haplotype structure around Bru1
reveals a narrow genec basis for brown rust resistance in modern
sugarcane culvars. Theorecal and Applied Genecs 125: 825-36.
Daros E, Oliveira RA and Barbosa GVS (2015) 45 anos de variedades RB
de cana-de-açúcar: 25 anos de Ridesa. Graciosa, Curiba, 156p.
Daros E, Oliveira RA, Zambon JLC, Filho JCB, Brasileiro BP, Ido OT, Ruaro L
and Weber H (2017) RB036088 – a sugarcane culvar for mechanical
planng and harvesng. Crop Breeding and Applied Biotechnology
17: 84-88.
Daros E, Oliveira RA, Zambon JLC, Filho JCB, Brasileiro BP, Ido OT, Ruaro
L and Weber H (2018) RB036066 – a sugarcane culvar with high
adaptability and yield stability to Brazilian South-Central region. Crop
Breeding and Applied Biotechnology 18: 325-329.
Eberhart SA and Russell WA (1966) Stability parameters for comparing
variees. Crop Science 6: 36-40.
Federer WT (1956) Augmented (or hoonuiaku) designs. Hawaian Planters’
Record 55: 191-208.
Kang MS, Miller JD and Tai PYP (1983) Genec and phenotypic path
analysis and heritability in sugarcane. Crop Science 23: 643-647.
Matsuoka S (1979) Método para pré-testagem de clones de cana-de-
açúcar ao carvão e ao mosaico conjuntamente. In I congresso
nacional da sociedade dos técnicos açucareiros e alcooleiros do
brasil. STAB, Maceió, p. 231-233.
Morais L K, Aguiar MS, Silva PA, Câmara TMM, Cursi DE, Fernandes
Júnior AR, Chapola RG, Carneiro MS and Bespalhok Filho JC (2015)
Breeding of sugarcane. In Cruz VMV and Dierig DA (eds) Industrial
crops: breeding for bioenergy and bioproducts. Springer, New
York, p. 29-42.
Prado H (2008) Pedologia fácil: aplicações na agricultura. Hélio do Prado,
Piracicaba, 45p.
Vieira MLC, Almeida CB, Oliveira CA, Tacuaá LO, Munhoz CF, Cauz-Santos
LA, Pinto LR, Monteiro-Vitorello CB, Xavier MA and Forni-Marns ER
(2018) Revising meiosis in sugarcane: chromosomal irregularies
and the prevalence of bivalent conguraons. Froners in Plant
Science 9: 213.
... The Federal University of São Carlos (UFSCar) had a sugarcane breeding program (PMGCA) recognized worldwide and that is part of the the Inter-University Network for the Development of Sugarcane Industry (RIDESA, www.ridesa.com.br). RIDESA is a a network of ten public Federal Universities that have successfully developed sugarcane cultivars adapted to different environments and agricultural managements enabling cultivation in more than 9 million hectares in Brazil , Daros et al. 2017, 2018, Carneiro et al. 2019. A new cultivar, RB985476, released by PMGCA/UFSCCar, has high tillering and excellent ratooning ability even under mechanized harvesting. ...
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