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A novel framework of viral whole genome analysis in an RNA virus
DJ Darwin Bandoy, Soﬁa Carolina Egana-Labrin, Rodrigo A. Gallardo, Bart C. Weimer
RNA viruses are hypermutable. Using reovirus as
model system for hypermutable virus evolution and
reassortment. Avian reovirus (ARV) in meat type
chickens manifests as a plethora of clinical signs
ranging from runting and stunting to a severe disease
characterized by viral tenosynovitis, pericarditis and
myocarditis. The strategy to control the disease in
meat type chickens entails breeder live virus
vaccination using conventional S1133-like strains,
followed by autogenous vaccines using prevalent
isolates obtained from the ﬁeld. However, the rate of
change in the virus hinders our ability to obtain
vaccines that provide persistent protection.
Kmer Distance Matrix
Whole Genome Sequencing
Viral Core Genome Hypothesis
Using a novel viral pangenomics approach to examine whole genome sequences of ARV, we
identiﬁed four core genes and 40 hypervariable regions, indicative of an open pangenome.
The core genes represent highly conserved regions across different ARV’s.
These changes may alter antigenicity and pathogenicity that maybe related to tissue tropism and
Using this approach with this virus is providing early indications that speciﬁc mutations can be
used in the design of next generation vaccines to control this virus in meat type chickens.