Article

Transformation of Soybean (Glycine Max L.) Via GUS –Labeled Agrobacterium Rhizogenes R1000

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Abstract

Ghada SH. AL-Yozbaki ; Jamella H. Rasheed ; Shifa M. Salih Biotechnology Lab., Department of Biology, College of Education for Pure Sciences , University of Mosul , Mosul, Iraq ABSTRACT The objective of this work was to investigate the ability of GUS –Labeled Agrobacterium rhizogenes strain R1000 for transformation to produce hairy roots in Glycine max L. .Two different methods were tested to perform the infection co-cultivation and direct inoculation . Cotyledons ,hypocotyls , epicotyls and leaves from seedlings of soybean were used as explants. In this study the direct inoculation was the best method .The cotyledons were showed a higher percentage for the formation of hairy roots, which were 76.9%, followed by hypocotyls , epicotyls and leaves which showed percentages 64.0% ,58.3% and 38.0% respectively .Histochemical assay proved that GUS- gene was transferred to hairy root cells .

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... Tỷ lệ mẫu hình thành rễ (%) là số mẫu hình thành rễ/số mẫu gây nhiễm. Tần số chuyển gene (%) là số mẫu hình thành rễ/số mẫu sống sót [7,11,12]. ...
... Trong trường hợp tỷ lệ mẫu sống sót là 100 %, tần số chuyển gene cũng chính là tỷ lệ mẫu hình thành rễ. Tỷ lệ mẫu sống sót càng cao thì tần số chuyển gene có giá trị càng gần với tỷ lệ mẫu sống sót [7,11,12]. Nhưng, do bản chất A. rhizogenes cũng chính là tác nhân gây bệnh cho thực vật, do đó tế bào thực vật cũng sẽ có những đáp ứng phòng thủ tương tự như khi nó tương tác với những yếu tố bất lợi khác [17,18]. Nếu không đáp ứng phòng thủ được, hậu quả là A. rhizogenes sẽ gây chết tế bào. ...
Article
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Catharanthus roseus is a well known medicinal plant. It produces several phytocompounds and many of which show anticancerous properties. However the yields of these compounds are very low. Recently, induction of C. roseus hairy roots by Agrobacterium rhizogenes, is interested as a promising tool for the enhanced production of these metabolites. In this research, wounded leaves from four strains of C. roseus were infected with various strains of A. rhizogenes isolated in Vietnam to provide more information about the induction efficiency of hairy roots. In this experiment, after 3 weeks of infection, the presence of rolB gene in hairy roots were analysed by polymerase chain reaction. All of 13 A. rhizogenes strains could induce the formation of hairy root in C. roseus. The A. rhizogenes C18 strain had the highest induction percentage in C. roseus VIN002, VIN005, and VIN072 with 59.4 %, 50.3 %, and 40.0 % respectively. And the same result was ontained at 26.7% by A. Rhizogenes C26 for C. roseus VIN077 rhizogenes strains. This result identified two A. rhizogenes strains C18 and C26 as potential transformation tools for hairy root production from C. roseus.
... Tỷ lệ mẫu hình thành rễ (%) là số mẫu hình thành rễ/số mẫu gây nhiễm. Tần số chuyển gene (%) là số mẫu hình thành rễ/số mẫu sống sót [7,11,12]. ...
... Trong trường hợp tỷ lệ mẫu sống sót là 100 %, tần số chuyển gene cũng chính là tỷ lệ mẫu hình thành rễ. Tỷ lệ mẫu sống sót càng cao thì tần số chuyển gene có giá trị càng gần với tỷ lệ mẫu sống sót [7,11,12]. Nhưng, do bản chất A. rhizogenes cũng chính là tác nhân gây bệnh cho thực vật, do đó tế bào thực vật cũng sẽ có những đáp ứng phòng thủ tương tự như khi nó tương tác với những yếu tố bất lợi khác [17,18]. Nếu không đáp ứng phòng thủ được, hậu quả là A. rhizogenes sẽ gây chết tế bào. ...
Article
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Catharanthus roseus is a well known medicinal plant. It produces several phytocompounds and many of which show anticancerous properties. However the yields of these compounds are very low. Recently, induction of C. roseus hairy roots by Agrobacterium rhizogenes, is interested as a promising tool for the enhanced production of these metabolites. In this research, wounded leaves from four strains of C. roseus were infected with various strains of A. rhizogenes isolated in Vietnam to provide more information about the induction efficiency of hairy roots. In this experiment, after 3 weeks of infection, the presence of rolB gene in hairy roots were analysed by polymerase chain reaction. All of 13 A. rhizogenes strains could induce the formation of hairy root in C. roseus. The A. rhizogenes C18 strain had the highest induction percentage in C. roseus VIN002, VIN005, and VIN072 with 59.4 %, 50.3 %, and 40.0 % respectively. And the same result was ontained at 26.7% by A. Rhizogenes C26 for C. roseus VIN077 rhizogenes strains. This result identified two A. rhizogenes strains C18 and C26 as potential transformation tools for hairy root production from C. roseus.
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Chapter
Legumes represent some of the most important crops in the world that are very useful in smallholder and large-scale farming systems. They are commonly referred to as the poor man’s meat due to their high dietary proteins and other micronutrients, including iron, zinc, folate, and thiamine that are important for human and livestock nutrition. The role of legumes in boosting food security and generating income in rural households across the globe is indispensable. Other than boosting healthy diet choices in rural households, legumes have also been used to promote soil health and fertility as they collectively account for 80% of biological nitrogen fixation. Industrial application of legumes is also gaining popularity with grain legumes currently used in synthesis of bioplastics. Legume production commands 27% of global crop production with a significant proportion coming from low input rural smallholder farms. However, legume production has declined over the years as a result of poor soil health management strategies and climate change. These have led to utilization of intensive agriculture practices that dictates the use of inorganic fertilizers and other agrochemicals to boost crop production. Intensive agricultural practices are unsustainable, costly and not eco-friendly. The plant growth-promoting microorganisms like rhizobia, mycorrhiza, and phosphate solubilizing bacteria are eco-friendly and can sustainably be used to improve soil fertility and improve legume production. Besides, co-inoculation of legume plants with rhizobia and phosphate solubilizing microorganisms can sustainably improve legume production and soil fertility. Here, we review the potential benefits of utilization of rhizobia, and other plant growth promoting microorganisms for sustainable improvement of legume production in smallholder agroecosystems.
... Quy trình gây nhiễm được thực hiện theo Yoshimatsu et al. (2004), Wang (2006) và AL-Yozbaki et al. (2015). Các mô lá thật từ cây Dừa cạn in vitro được tạo vết thương bằng dao mổ ngang qua gân chính của lá. ...
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Chapter
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Transgenic roots from Catharanthus roseus by Agrobacterium rhizogenes can synthesize many alkaloid compounds having costly pharmaceutical properties. Induction of transgenic roots in C. roseus depended on various factors. Infection of leaves of three C. roseus strains at difference of OD 600nm of A. rhizogenes C18 suspension, infection time, co-cultivation period, and light indicated these factors effected severally on the root forming induction. The OD 600nm of suspension, infection time, and co-cultivation period were three positive factors for formation of roots at the certain conditions. Whereas, illumination with white light in co-cultivation period could inhibit transgenic root induction of A. rhizogenes C18 in all of three C. roseus strains.
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