Thesis

Proximate Analysis and Detection, Isolation and Characterization of Phenolic Lipids from Mangifera altissima Fruit

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Abstract

Mangifera altissima fruit peel, flesh, seed and latex were analyzed to detect, isolate, and characterize phenolic lipids. Initially, the fruit parts were dried and powdered. Proximate analysis showed that M. altissima flesh showed the highest values in the percent ash, percent crude protein, percent fat analyses, while the M. altissima seed showed highest values in percent moisture content and total carbohydrates and M. altissima peel showed highest values in percent crude fiber.The powdered samples were extracted with 1:1 mixture of dichloromethane/ethanol using Soxhlet appratus at 40° for 8 hours. The extracts formed a blue-colored solution upon confirmatory test using FeCl3. Using the Folin-Ciocalteu method, the total phenol content of M. altissima fruit peel, flesh, seed and latex extracts were quantitated. The latex showed the highest amount of total phenolics with an average of 57.95 ± 6.90 mg GAE/g. The mango fruit DCM extract was purified using column chromatography and the six fractions obtained were subjected to 1HNMR. The spectra have almost similar fingerprints, showing distinctive peaks in the aromatic region. In some of the spectra, signals in the aldehydic region, were detected. A rough structure was elucidated using the spectral data.

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