Effect of Fennel Extract on the Improvement of Memory Disorders in Beta Amyloid Alzheimer Model of Male Wistar Rats

Abstract

Introduction: Alzheimer's disease is the most common neurodegenerative disorder. Due to the neurodegenerative effect of fennel (Foeniculum vulgare) this study aimed at investigating the effect of this plant on the improvement of learning and memory in rat models of Alzheimer's disease.

Material & Methods: The male Wistar rats were assigned into six groups, namely the control group (no injection), sham group (saline injection), experimental group (injection of beta-amyloid (40-1). The other three groups received 75, 140, and 250 ml/kg of fennel extract intraperitoneally 7 days before and 3 weeks after bilateral intraventricular beta-amyloid injection. Shuttle box was used to study the learning and memory behavior of the rats; moreover hematoxylin-eosin staining was conducted for histological studies.

Findings: According to the results, fennel extract at a dose of 140 mg/kg increased behavioral indicators in measuring the passive avoidance memory in the Alzheimer's group, compared to the sham group (P

Full text

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              
Foeniculum vulgare
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     
neurodegenerative
      

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  
       

    
     

       

beta-amyloid (Aβ)

 

        
    
       

 

    
    
      
 reactive oxygen spices (ROS)
   
 Aβ 
    ROS 
     



    
        

     
     


   
       
  


       
  
CA1
  

   
Foeniculum vulgar Mill Apiaccac
     
   
    

   

     
    
  
       
     

  
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        
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        
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Downloaded from sjimu.medilam.ac.ir at 6:23 IRDT on Tuesday June 25th 2019 [ DOI: 10.29252/sjimu.27.1.1 ]

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     
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   
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Aβ
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     
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     
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      
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      
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  
   
      
      
    ±
     
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     
       
        
      
    
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       

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   

        
        
         
       
        
      
 .      
      
        
       . 
         
      
         
   
       
         
    .    
    
        
        
      
      
       
    . 
      
        
Downloaded from sjimu.medilam.ac.ir at 6:23 IRDT on Tuesday June 25th 2019 [ DOI: 10.29252/sjimu.27.1.1 ]



       
        
        
        
      
     .     
       
          
          
      
   

     
 
Cambridge Medical Instruments,
United Kingdom    
        
  -  
       
      
       
    .   
       
    
 Olysiabioreport 
 

   SPSS     
ANOVAT-test
PTukey-testPost
hocP<0.05
    
      Sigma plat


      


        
       
      
     
P<0.001 




Aβ (1-40)Aβ (1-40))P<0.001###( (n=8)
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 
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        
        
   
 P<0.05    
       
  
   
 P<0.05  
  




Aβ (1-40)P<0.05#, P<0.05***(


     

        

 





   
       
   

    P<0.001

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
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


P<0.001
P<0.001###

     
 
  
       

        


    
       
     

      
    


      
    
  
P<0.001




P<0.001***
P<0.001###
Downloaded from sjimu.medilam.ac.ir at 6:23 IRDT on Tuesday June 25th 2019 [ DOI: 10.29252/sjimu.27.1.1 ]

 


    &
    
CA1

      
    CA1




         



       
CA1
      
       

CA1

     
      
   Aβ (1-40) 
     
CA1
AC

  
   CA1

     
       

       
    

CA1


Aβ
(1-40)B


CA1

 

       
E


CA1
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   
     

Aβ (1-40)
 

 
       
      
     
     Aβ (1-40)
EDF
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
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H&ECA1AAβ (1-40)B
mg/kgFV 70Dmg/kgE FV 140 
mg/kgFV 250 F
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
   
     CA1

     

   
     



CA1

       
   Aβ (1-40)
       
 
   

        
    

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CA1A
Aβ (1-40)Bmg/kgFV 70Dmg/kgE FV 140 
mg/kgFV 250 F
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 
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
       
        
   1-40Aβ  
       

      

       



    
    

    

    

     
    
Aβ (1-40)
  
Aβ
   


α

 Aβ    
  AD 
   
       
        
     

     CA1
 

      


    
Woolly 
    CA1  
 
      
  NO    
NO
      
      
    
   β 
      
    
     
   β
 NT2
  PC12

 H2O2

   
    



       
Visual

   
    

     
   


     

   
Downloaded from sjimu.medilam.ac.ir at 6:23 IRDT on Tuesday June 25th 2019 [ DOI: 10.29252/sjimu.27.1.1 ]



      

      



         
       
   
       

Aβ (1-40)

CA1
 

    
Aβ(1-40)

  
       

     
     


Aβ (1-40)
CA1
  
Aβ (1-40)

CA1
CA3
DG  

      
Aβ (1-40)
CA1

     
     
      

  RNA
 
   
  CA1
  
Aβ (1-40)









     
     
     


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

Effect of Fennel Extract on the Improvement of Memory
Disorders in Beta Amyloid Alzheimer Model
of Male Wistar Rats
Eslimiesfahani D1*, Oryan S1, Khosravi M1, valizadegan F2
(Received: January 6, 2018 Accepted: May 28, 2018)
Abstract
Introduction: Alzheimer's disease is the
most common neurodegenerative disorder.
Due to the neurodegenerative effect of
fennel (Foeniculum vulgare) this study
aimed at investigating the effect of this
plant on the improvement of learning and
memory in rat models of Alzheimer's
disease.
Material & Methods: The male Wistar rats
were assigned into six groups, namely the
control group (no injection), sham group
(saline injection), experimental group
(injection of beta-amyloid (40-1). The other
three groups received 75, 140, and 250
ml/kg of fennel extract intraperitoneally 7
days before and 3 weeks after bilateral
intraventricular beta-amyloid injection.
Shuttle box was used to study the learning
and memory behavior of the rats; moreover
hematoxylin-eosin staining was conducted
for histological studies.
Findings: According to the results, fennel
extract at a dose of 140 mg/kg increased
behavioral indicators in measuring the
passive avoidance memory in the
Alzheimer's group, compared to the sham
group (P<0.001). Moreover, optical
microscopy studies showed a significant
decrease in tissue damage and an increase
in the number of Nissl bodies stained in
CA1 hippocampal region, especially in
pyramidal layer, in Alzheimer's disease-
induced rats treated with fennel extract at a
dose of 140 mg/kg.
Discussion & Conclusions: In this study,
fennel extract shows the neuroprotective
effect against Alzheimer’s disease induced
by beta-amyloid (40-1). the observed
improvements are probably related with
antioxidant, anti-inflammatory, and pseudo-
estrogenic properties of this plant.
Keywords: Alzheimer’s disease, Beta-
amyloid, Passive avoidance memory, Rat,
Shuttle box
1. Dept of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
2. Dept of Biology, Faculty of Sciences, Mazandaran University, Babolsar, Iran
* Corresponding author Email: eslimid@yahoo.com
Scientific Journal of Ilam University of Medical Sciences

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