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Characterization of a murine model of non-lethal, symptomatic dengue virus infection
Vanessa V. Sarathy, Mellodee White, Li Li, Jaclyn A. Kaiser, Gerald A. Campbell, Gregg N.
Milligan, Nigel Bourne, and Alan D.T. Barrett
Supplementary Table 1: FRNT50 values of six-week-old AG129 mice infected with DENV-3.
^Reported in Sarathy, 201512.
D83-144 C0360/94
Inoculum 107.0 108.0 ^106.5 107.0 ^107.5
n2 4 8 1 1
FRNT50
(95% CI)
1540
(1140-2082)
1118
(905-1379)
399
(338-472)
1279
(1077-1518)
634
(546-736)
r20.90 0.91 0.88 0.96 0.99
dpi 52 28 29 52 29
Supplementary Figure 1
Supplementary Figure 1: Histology and immunostaining of liver sections from C0360/94-
infected mice. H&E staining of liver sections from animals sacrificed on days 1 (a,b), 2 (d,e),
and 3 (g,h) post-infection (p.i.) with a lethal dose of DENV-3 C0360/94 shows damage in the
liver sections. 10X (a, d, g) and 40X (b, e, h) magnifications show broad and localized effects on
liver tissue. (c, f, i) Immunostaining for DENV NS3 (red) in liver sections indicates that
C0360/94 actively replicates in the liver during the course of infection on days 1-3. Blue staining:
nuclei, with DAPI.