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We explored the potential application of sulforaphane against influenza A virus and elucidated the underlying cytopathic effect (CPE) and cytotoxicity. In the present study, 2 sulforaphane products were used to investigate the CPEs on influenza A virus replication in Madin-Darby canine kidney cells and to conduct a cytotoxicity test. One was the standard sample and the other was extracted from broccoli seeds. The 2 products of sulforaphane were each diluted to different concentrations. The results indicated that sulforaphane possessed antiviral activity against influenza A/WSN/33 (H1N1) virus, and the standard sulforaphane sample showed biological activity against influenza virus with low cytotoxicity at concentrations of 6.25 to 12.5 μM. The same phenomenon was observed with a broccoli seed extract concentration of sulforaphane of 6.25 μM. Both samples displayed higher cytotoxicity at 50 μM of sulforaphane, and the extract samples showed stronger cytotoxicity at sulforaphane concentrations of 12.5 to 100 μM compared with the standard, particularly at 100 μM. In conclusion, natural sulforaphane from broccoli seeds showed potential as an agent against influenza A virus infection, and the CPE after treatment with sulforaphane was concentration dependent; a suitable sulforaphane concentration of 6.25 μM is suggested and was demonstrated as effective, with high antiviral activity and low cytotoxicity.
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1Institute of Vegetables and Flowers, Chinese Academy of Agricultural
Sciences, Beijing, P.R. China
2Key Laboratory of Biology and Genetic Improvement of Horticultural
Crops, Ministry of Agriculture, Beijing, P.R. China
Corresponding Author:
Zhansheng Li, Key Laboratory of Biology and Genetic Improvement of
Horticultural Crops, Ministry of Agriculture, Beijing, P.R. China.
Email: lizhansheng@ caas. cn
Original Article
Natural Product Communications
June 2019: 1– 8
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Natural Sulforaphane From Broccoli
Seeds Against Influenza A Virus
Replication in MDCK Cells
Zhansheng Li1,2, Yumei Liu1,2, Zhiyuan Fang1,2, Limei Yang1,2, Mu Zhuang1,2,
Yangyong Zhang1,2, and Honghao Lv1,2
We explored the potential application of sulforaphane against influenza A virus and elucidated the underlying cytopathic ef-
fect (CPE) and cytotoxicity. In the present study, 2 sulforaphane products were used to investigate the CPEs on influenza A
virus replication in Madin-Darby canine kidney cells and to conduct a cytotoxicity test. One was the standard sample and the
other was extracted from broccoli seeds. The 2 products of sulforaphane were each diluted to different concentrations. The
results indicated that sulforaphane possessed antiviral activity against influenza A/WSN/33 (H1N1) virus, and the standard
sulforaphane sample showed biological activity against influenza virus with low cytotoxicity at concentrations of 6.25 to 12.5
μM. The same phenomenon was observed with a broccoli seed extract concentration of sulforaphane of 6.25 μM. Both
samples displayed higher cytotoxicity at 50 μM of sulforaphane, and the extract samples showed stronger cytotoxicity at
sulforaphane concentrations of 12.5 to 100 μM compared with the standard, particularly at 100 μM. In conclusion, natural
sulforaphane from broccoli seeds showed potential as an agent against influenza A virus infection, and the CPE after treat-
ment with sulforaphane was concentration dependent; a suitable sulforaphane concentration of 6.25 μM is suggested and was
demonstrated as effective, with high antiviral activity and low cytotoxicity.
sulforaphane, broccoli, cytotoxicity, influenza virus
Received: December 11th, 2018; Accepted: March 20th, 2019.
Inuenza A virus, a member of the Orthomyxoviridae fam-
ily, is a major human pathogen that typically causes annual
epidemics and occasional pandemics.1-3 Furthermore, the
recent emergence of highly pathogenic avian and swine u
has become a global issue for humans.4,5 In the past 100
years, 5 inuenza epidemics and pandemics have caused
increasing morbidity and mortality worldwide, including
H1N1 in 1918, H2N2 in 1957, H3N2 in 1968, H5N1 in 2009,
and H7N9 in 2014. Particularly, inuenza A (H7N9) causes
high mortality in China.2,6,7 Inuenza A virus is an RNA
virus with a rough spherical shape. These viruses are nega-
tive sense, single-stranded, and segmented with 7 or 8 pieces,
and each piece encodes 1 or 2 genes. Two large glycopro-
teins, hemagglutinin (HA) and neuraminidase (NA), have
been identied in inuenza A viruses of dierent subtypes on
the surface of the viral envelope. Thus far, 18 types of HA
and 11 types of NA have been identied among dierent
inuenza viruses.8,9
HA is a glycol protein expressed on the viral surface along
with NA, and these proteins are responsible for the
attachment of the viral particle to the host cell through cell
surface sialic acid receptors.3,10 HA subsequently completes
the fusion of the viral envelope with the host membrane to
release the viral genome into the target cells, initiating infec-
tion. NA is an enzyme that cleaves the sialic acid residue
tethering the progeny virus and detaches it from infected
cells, accomplishing virus propagation.10,11 Currently, 2
main strategies, using vaccination and anti-inuenza drugs,
are widely employed. However, eective vaccination may
require sustained reproduction to match the antigenic
Natural Product Communications2
changes of viruses, implying that it is almost impossible to
produce ecient and timely vaccines to control inuenza
outbreaks.12,13 Currently, 2 classes of anti-inuenza drugs,
amantadine and rimantadine, have been developed for the
interruption of certain processes of inuenza infection by
targeting either the M2 channel or NA.3,12 Additionally, osel-
tamivir (OSV) and zanamivir target NA protein by the inhi-
bition of its enzymatic activity, rendering the tethered
progeny virus unable to escape from the host cells.14
However, resistant variants have continued to emerge from
patients after treatment, regardless of both classes of drugs
and other drugs, making it urgent to identify novel anti-inu-
enza drugs with safe and eective activity.3,15 Sulforaphane
[1-isothiocyanato-4-(methylsulnyl) butane] is an isothiocy-
anate produced by the hydrolysis of glucoraphanin-rich
broccoli (Brassica oleracea var. italica).16-19 Epidemiological
studies have shown that sulforaphane exhibits anticancer
activity, cardiovascular disease prevention, hypotensive
eects, and myopia prevention.20,21 The anticancer activities
of sulforaphane have been widely demonstrated and studied
in many cancers, including those of the lung,22 stomach,19
liver,23 colon,24 breast,25 prostate,20 and bladder.26 This
mechanism of sulforaphane is attributed to its induction of
phase II detoxication enzymes and prevention of the gener-
ation of phase I detoxication enzymes and
Many studies have reported that sulforaphane plays a key
role in sulforaphane preconditioning of the Nrf2 defense
pathway, which protects cerebral vasculature against blood-
brain barrier disruption, neurological decits in stroke, and
tumor growth and spread.29 Additionally, in the past 30 years,
studies have shown that sulforaphane plays an important role
in regulation and as an inducer of Nrf2 signaling and ecacy
as an inhibitor of carcinogenesis, as well as preventing infec-
tious disease, cardiovascular disease, and recently new med-
ical areas.30 Measures of the Nrf2 pathway response and
function serve as guideposts for the optimization of dose,
schedule, and formulation as clinical trials with sulforaphane
and broccoli-based preparations have become more com-
monplace and more rigorous in design and implementa-
tion.3,12 Thus, it is necessary to investigate the response of
standard sulforaphane and the broccoli extract sulforaphane
against HA cells, which can aid in new drug research and
support human health via the consumption of broccoli.19,22,31
Thus far, few reports have shown whether sulforaphane
could prevent inuenza virus infection; therefore, it is neces-
sary to explore this issue. In the present study, inuenza A/
WSN/33(H1N1) virus was selected to examine sulforaphane
in cytopathic eect (CPE) reduction assays and cytotoxicity
tests, which are benecial for research on inuenza preven-
tion, the development of new drugs, and epidemiology
In the present study, we selected broccoli seeds with high
sulforaphane content as material for extracting sulforaphane.
The sulforaphane content in broccoli seeds was 5512.63
mg/L DW, as detected by HPLC, which is a high content in
Brassica.32,33 The chromatography of sulforaphane in the
standard and extracted samples showed that the system was
eective and stable. The linearity was good within the range
of 5.0 to 300.0 mg/L, and the linear equation was Y = 2.76 ×
10-4X −0.73 (R2 = 0.9998), where the X-axis represents the
peak area and the Y-axis represents the concentration (mg/L).
The HPLC method, with low-temperature working condi-
tions and good stability, has been widely used for the deter-
mination of sulforaphane and glucosinolates and their
hydrolysis products.33,34 Sulforaphane, the second product
derived from glucoraphanin, is not stable and easily changes
into either nitrile material or other compounds at high tem-
peratures or under dierent chemical conditions.33 Thus, GC
and GC-MS are not suitable for the determination of sulfora-
phane or glucosinolates. Most studies have successfully
detected sulforaphane in cruciferous vegetables, particularly
in B. oleracea, with HPLC and HPLC-MS methods.
Sulforaphane is particularly rich in broccoli; the ripe seed
contains the highest levels, followed by seedlings, buds, o-
rets and stems, and leaves, and almost no sulforaphane has
been detected in the roots.33 Thus, HPLC and HPLC-MS
have become typical and simple determination methods for
Initially, we examined the cytotoxicity of sulforaphane in
Madin-Darby canine kidney (MDCK) cells by using the
CellTiter-Glo® assay. Culture medium containing 0.5%
dimethyl sulfoxide (DMSO) served as a negative control.
The compound showed cytotoxicity in MDCK cells at con-
centrations of more than 25 µM, while the compounds did
not show signicant cytotoxicity to MDCK cells at concen-
trations of less than 12.5 µM (Figure 1). As shown in
Figure 1. The induction of viral resistance was examined
by continuous sulforaphane treatment. Plaque formation was
observed at different concentrations of sulforaphane (6.25, 12.5,
25.0, 50.0, and 100 μM) by microscopy. (a) Standard, (b) the
broccoli seed extract. Madin-Darby canine kidney cells were
infected with influenza A/WSN/33 (MOI = 0.01) and treated with
sulforaphane and dimethyl sulfoxide. At 40 hours postinfection,
the supernatants were collected and used for infection in the next
round of investigation. Virus yields of mock-treated cells were set
at 100%.
Li et al. 3
Figure 1, concentrations of 6.25 and 12.5 µM of sulfora-
phane showed signicant bioactivity against inuenza A
virus, and the cytotoxicity in MDCK cells was not signi-
cantly dierent from that of the control.
CPE screening, an assay for measuring the damage to
host cells during virus invasion, was utilized to screen and
identify compounds that display a reduction in the CPE on
inuenza A/WSN/33 virus.2 We found that 6.25 and 12.5 µM
concentrations of standard sulforaphane showed signicant
bioactivity against inuenza A virus (Figure 1a), and the
cytotoxicity to MDCK cells was not signicantly dierent
from that of the control. The reduction in CPE was conrmed
by direct microscopic observation, which detected far less
CPE than in the DMSO control (Figure 1). In addition, this
compound exerts a well-dened dose-dependent response
against the A/WSN/33 virus based on plaque formation
(Figure 1). This is an alternative assay for the evaluation of
potency, with an EC50 of approximately 6.25 µM, which is
almost 2-fold lower than that of OSV.3,36 The same eect
was observed at a concentration of 6.25 µM of sulforaphane
seed extract (Figure 1b), consistent with that of the standard
According to microscope observations, there were dier-
ent reections of MDCK cells alone and those infected with
virus to dierent gradient concentrations of sulforaphane
diluted with DMSO based on the same magnication
(Figure 1). MDCK cells with virus showed a greater reduc-
tion in CPE at sulforaphane concentrations of 100 and 50
µM than that of standard with more transparent cells. The
extract from broccoli seed showed higher CPE reduction at
sulforaphane concentrations of 100, 50, and 25 µM, and both
samples demonstrated that sulforaphane could prevent inu-
enza A virus, especially at low concentrations of 12.5 and
6.25 µM, compared with the control without virus (DMSO).
The most characteristic feature of sulforaphane is its high
chemical reactivity due to the electrophilicity of the central
carbon of the isothiocyanate (-N=C=S) group,37 which read-
ily reacts with sulfur-, nitrogen-, and oxygen-centered nucle-
ophiles.38 Chemical modication of the sensor cysteine of
KEAP1 by sulforaphane impairs its substrate adaptor func-
tion, leading to Nrf2 accumulation and the enhanced tran-
scription of Nrf2-dependent genes.38 These genes have
antioxidant response elements (AREs) in their upstream reg-
ulatory regions, which are the sites of binding Nrf2 as a het-
erodimer with a small Maf transcription factor.39
Nrf2-dependent genes encode multiple functionally
diverse enzymes and other proteins with cytoprotective
activities.29,30 Several studies have indicated that there is an
inverse relationship between the levels of Nrf2 expression
and the viral entry and replication, and an attractive thera-
peutic intervention demonstrated that supplementation with
Nrf2-activating antioxidants inhibits viral replication in
human NEC.29,40 Sulforaphane could increase the accumula-
tion of Nrf2; therefore, additional studies are needed to
explore the relation between the virus and standard sulfora-
phane or its extract from broccoli.21,40
Studies have reported that glucoraphanin is also taken up
from the gut to the liver where it is interconverted to its
reduced glucosinolate analog, glucoerucin, while sulfora-
phane is converted to its corresponding reduced isothiocya-
nate analog, erucin [1-isothiocyanato-4-(methylthio)
butane].41,42 Glucoerucin and glucoraphanin have poor bio-
availability; thus, the bioavailability found in humans or
other mammals is typically due to sulforaphane in a specic
test.43 These ndings suggested that sulforaphane at concen-
trations of 6.25 and 12.5 µM were good for living cells and
eective against inuenza A virus. Higher cytotoxicity was
observed at concentrations higher than 25.0 µM in both the
standard and extracted samples (Figure 1). Moreover, the
extract contained more toxic compounds from the extraction
process than the standard, which should be investigated in
future research.
We examined the inhibitory activity of the test compounds
against virus replication in MDCK cells using the inuenza
A/WSN/33 (H1N1 subtype) virus strain. The results are
shown in Figure 2, including OSV as a positive control. The
compounds showed signicant anti-inuenza A/WSN/33
virus activity at 25, 12.5, and 6.25 µM of the standard sul-
foraphane, and 6.25 µM of the sulforaphane from broccoli
seed extract (Figure 2) (P < 0.05).
The MDCK cells without the inuenza A virus (control)
indicated that DMSO and OSV showed no signicant cyto-
toxic activity on MDCK cells (Figure 2), whereas 6.25 µM
Figure 2. The cytotoxicity toward influenza A virus in Madin-
Darby canine kidney cells treated with different concentrations
of sulforaphane from the standard and extract samples. The
capital letters show significant differences at the level of 0.01
based on one-way ANOVA. Capital letters shown in red and
black represent parallel comparisons of black bars and red bars,
Natural Product Communications4
sulforaphane of the standard slightly reduced the activity of
MDCK cells maintaining 90.2% of initial cell numbers
(DMSO). Notably, 12.5 µM standard sulforaphane and 6.25
µM sulforaphane in the extract from broccoli seed showed
the same level of activity, maintaining 81.7% and 77.0% of
initial cell numbers, respectively. A concentration of 25.0
µM standard sulforaphane maintained 67.0% of initial cell
numbers, and both 100 µM standard sulforaphane and 12.5
µM sulforaphane in the extract from broccoli seed showed
the same level of activity (57.5% and 55.3%, respectively,
responding to initial cell numbers). This result suggested that
sulforaphane is eective against inuenza (EC50 > 12.5 µM).
The concentration of 50.0 µM sulforaphane in the extract of
broccoli seed was better than 50.0 µM sulforaphane standard
and 25.0 µM sulforaphane in the extract of broccoli seed
with regard to cytotoxic activity for MDCK cells. The 100.0
µM concentration of sulforaphane in the extract from broc-
coli seed showed strong cytotoxic activity toward MDCK
cells, maintaining only 6.5% of initial cell numbers.
The positive OSV control treatments showed that the
numbers of MDCK cells infected with inuenza A virus and
treated with the OSV positive control (Figure 2), 6.25 µM
sulforaphane standard and in the extract samples, and 12.5
µM sulforaphane standard were higher than the numbers of
MDCK cells treated with DMSO (negative control), suggest-
ing that sulforaphane at a concentration of 6.25 µM from
both standard and broccoli extract showed better bioactivity
against inuenza A virus in MDCK cells. Standard and
extract samples showed 19.2% and 17.8% increased cell
numbers, respectively, compared with DMSO-treated cells,
and the positive control increased cell numbers by 40.0%.
The same function against virus was also found at 12.5 and
6.25 µM sulforaphane, while 25 µM sulforaphane standard
had no signicant eect on MDCK cells infected with inu-
enza A virus compared with the negative control. Additionally,
100 µM of sulforaphane standard and 12.5 µM sulforaphane
in the extract from broccoli seed showed the same eect,
maintaining 84.0% and 81.4% of initial cells (DMSO),
respectively. Moreover, 50 µM sulforaphane standard or in
the extract samples maintained 65.6% and 63.9% of the ini-
tial cells (DMSO), respectively, and 25.0 µM sulforaphane
standard could maintain 55.0% of the initial cells, whereas
100 µM sulforaphane in the extract only maintained 7% of
the initial cells, suggesting strong cytotoxicity to MDCK
During the hydrolysis of glucoraphanin, more sulfora-
phane product is catalyzed by myrosinase at neutral or high
pH, in the presence of Zn2+ at low temperature. In contrast,
at acidic pH, in the presence of Fe2+ or Cu2+, and at high
temperature, nitrile will be favored.44,45 According to the
observed eects of the sulforaphane standard on MDCK
cells, we concluded that sulforaphane was the main compo-
nent, but there might be a small amount of nitrile or residual
product(s) from broccoli seeds in the extract, which could be
present in samples of a higher concentration, causing
toxicity to MDCK cells (Figure 2). Moreover, many studies
have demonstrated and validated that sulforaphane is readily
absorbed in humans or mammals and is rapidly eliminated,
and more than 70% of the administered dose of sulforaphane
can be recovered as thiol conjugates in the urine with a bio-
logical half-life of only a few hours, providing further evi-
dence of sulforaphane with no signicant toxicity to animals
and mammals.46 Additionally, according to the cytotoxicity
test activity of inuenza A virus treated with dierent con-
centrations of sulforaphane from the standard and extract
samples, except for the 100 µM concentration of sulfora-
phane derived from the extract with higher cytotoxicity to
MDCK cells, low concentrations of sulforaphane (6.25–50
µM) from the standard and the extract showed lower cyto-
toxicity to MDCK cells (C and C+V). Moreover, some addi-
tional chemical compounds in the sulforaphane extract
contributed to the cytotoxicity to MDCK cells.
This result suggested that the cytotoxicity test activity of
inuenza A virus treated with dierent concentrations of sul-
foraphane in MDCK cells demonstrated concentration
dependence, and the concentrations of 6.25 to 12.5 µM based
on the standard were eective for MDCK cell activity with
no obvious cytotoxicity. Additionally, the purication meth-
ods, such as preparative liquid chromatography and DEAE-
Sephacel, could be used for better purication of sulforaphane
before application in food engineering.44,45
HA represents an attractive target for discovering new
anti-inuenza agents because of its popular role in host cell
attachment and fusion processes.10 Recent reports reveal that
several compounds with small molecules can interfere with
the HA functions by hindering one or both functions. The
major function is binding to the receptor-binding site and
competing with sialic acids, such as triterpenoids,2,12 and
sialic acid mimetic peptides.8 Recently, arbidol has been
commercialized in China,4,47 and stachyin derivatives,
CL-61917, polyphenols, BMY 27709, and some others have
recently been explored.48
The Nrf2 signaling pathway can regulate N600 genes, of
which N200 encodes cytoprotective proteins that are also
associated with inammation, cancer, neurodegenerative
diseases, and other major diseases. Recently, Nrf2 expres-
sion was shown to modify inuenza A entry and replication
in nasal epithelial cells, which provided good cross evidence
for exploring the function of sulforaphane in anti-inuenza A
virus.21,29 Recently, several studies have focused on extract-
ing sulforaphane from broccoli seeds and sprouts to examine
its anti-cancer activity, showing that sulforaphane and sul-
foraphane broccoli extract have the same or similar functions
against cancers.49
Since the recognition of the bioactivity of sulforaphane in
1992,2,12 several studies have examined its action in cells,
animals, and humans. Additionally, increasing evidence has
shown that broccoli, particularly as seeds and young sprouts,
is a rich source of sulforaphane, and broccoli-based prepara-
tions are now used in clinical studies probing their ecacy in
Li et al. 5
health preservation and disease mitigation.23 The transcrip-
tion factor Nrf2 is a master regulator of cell survival
responses to endogenous and exogenous stressors.50 Studies
have revealed that many putative cellular targets are aected
by sulforaphane, although only KEAP1-Nrf2 signaling can
be considered a validated target at this time.21,29 Thus, we
propose that sulforaphane plays a role in preventing HA by
interfering in the Nrf2 signaling pathway, which provided
the premise for the present study. In addition, sulforaphane
has therapeutic eects on inammatory diseases through the
Nrf2 signaling pathway.51,52
Currently, sappanone, an anti-inuenza, antiallergic, and
neuroprotective medication, is widely distributed in
Southeast Asia.53 Bixin extracted from the seeds of Bixa
orellana is used to treat infectious and inammatory diseases
in Mexico and South America,54 and both sappanone and
bixin play medical roles via Nrf2-dependent mechanisms.
According to the above-mentioned studies and other recent
reports, sulforaphane has activities against cancer, inamma-
tion, cardiovascular disease, and neurological diseases and
improves immunity.27,37 Thus, sulforaphane might play an
important role as an anti-inuenza virus by increasing the
accumulation of Nrf2 factors and decreasing the replication
of the virus. These plant compounds activate the Nrf2 signal-
ing pathway mainly in the form of electrophilic materials
that modify the cysteine residues of KEAP1, leading to free
nuclear Nrf2 binding with the ARE, resulting in Nrf2 accu-
mulation and the activation of the transcription of the corre-
sponding genes.2,55,56
Cells and Materials
MDCK cells were grown in Dulbecco’s modied Eagle
medium (DMEM) (Gibco BRL, Inc., Gaithersburg, MD,
USA) supplemented with 10% fetal bovine serum (FBS)
(PAA Laboratories, Linz, Austria) at 37°C under 5% CO2.
Inuenza A/WSN/33 (H1N1) virus, provided by HAKE
Genetics Co., Ltd., was used in the present study. “WSN” is
the acronym for the inuenza A/Wilson Smith/1933 (H1N1)
neurotropic variant, which was deliberately selected by
repeatedly passaging its parent virus, inuenza A/Wilson
Smith/1933 (H1N1) virus (WS), in mouse brain. The WS
virus was isolated in 1933 by Wilson Smith and colleagues
from human inuenza by inoculating ferrets.2 Broccoli
“B61” seeds were cultured at the Institute of Vegetables and
Flowers, Chinese Academy of Agricultural Sciences, and
subsequently collected for extracting the bioactive com-
pound sulforaphane.
Sulforaphane standard was purchased from LKT Labs (LKT
Laboratories, Inc., St Paul, MN, USA), and the purity was
more than 98% (HPLC grade). Methanol, ethyl acetate, and
DMSO were obtained from Sigma (Sigma Chemical
Company, St Louis, MO, USA). Phosphates were purchased
from Beijing Chemical Company (Beijing, China). The stan-
dard samples were dissolved in 10 mL of DMSO (Sigma
Chemical Company) with a concentration of 1.0 g/L and
then serially diluted to concentrations of 6.25, 12.5, 25, 50,
and 100 µM for CPE assays and cytotoxicity tests; another
concentration gradient was used to determine the linearity of
HPLC Conditions
The Shimadzu LC-20A HPLC system was equipped with an
SPD-20 UV detector and a reverse-phase C18 column (250 ×
4.6 mm, 5 µm, Shiseido, Japan). The gradient mobile phase
consisted of 5% tetrahydrofuran for pump A and 100% meth-
anol for pump B. The solvent for pump B was initially set at
40%, then linearly changed to 60% by the 10th minute, and
subsequently returned to full methanol (100%) after an addi-
tional 10 minutes, maintained at 100% for 15 minutes at a
ow rate of 0.80 mL/min, and nally returned to the initial
condition. The absorbance value was 254 nm, and the col-
umn oven temperature 32°C. A total of 10 mg of the sulfora-
phane standard was dissolved in 10 mL of methanol to
generate a dilution series: 5.0, 50.0, 100.0, 200.0, and 300.0
mg/L. The precision of the system was measured by standard
peak areas (n = 6, 100 mg/L), and the recovery was dened
by adding standard samples (100 mg/L) at known concentra-
tions (5.51, 12.55, 20.43, 45.51, 60.27, 80.69 mg/L) (n = 6).
The determination method was performed as previously
described,57 and this method is popularly used for the deter-
mination of sulforaphane in plants.32
Extraction of Sulforaphane
The extraction method was performed according to Liang,44
with some modications, as detailed in previous studies.32,33
A total of 0.5 g of seed powder was homogenized in 15 mL
of neutral phosphate buer (0.1 M and pH 7.0). The homog-
enate was then transferred to a beaker with stirring for 2
hours, after which 30 mL ethyl acetate was injected. Thirty
minutes later, the mixture was centrifuged for 10 minutes at
6000 × g (Thermo, MA, USA). The supernatant in the tubes
was collected, and the remaining mixture was transferred to
a fresh beaker. Then, 30 mL of ethyl acetate was added to the
mixture and treated by the initial process. The same opera-
tion was repeated again. Finally, the 3 supernatants were
evaporated in a rotavapor (RII, BÜCHI TM, Switzerland) at
35°C. The residue was then dissolved in 10 mL of methanol
and ltered through a 0.22 µm (D 13 mm) nylon lter paper
(Agela, China). The solution was stored at -20°C until HPLC
CPE Reduction Assay
This assay was performed as previously described,2 with
some modications. MDCK cells were seeded onto 96-well
Natural Product Communications6
plates, incubated overnight, and infected with inuenza virus
(MOI = 0.1) suspended in DMEM supplemented with 1%
FBS, containing test compound and 2 mg/L TPCK-treated
trypsin, with a nal DMSO concentration of 1% in each
well. After 40 hours of incubation, CellTiter-Glo reagent
(Promega Corp., Madison, WI, USA) was added, and the
plates were read using a plate reader (Tecan Innite M2000
PRO; Tecan Group Ltd, Mannedorf, Switzerland). The CPE
in virus-infected cells was observed through microscopy.
MDCK cells were infected with inuenza A/WSN/33 (MOI
= 0.01) and treated with dierent concentrations of sulfora-
phane from the standard and extract samples diluted with
DMSO to 6.25, 12.5, 25, 50, and 100 µM. Each treatment
was designed in triplicate (n = 3). The postinfection superna-
tants were collected and used for infection in the next round
of investigation.3
Cytotoxicity Test
Cells grown on 96-well plates overnight were cultured in 1%
FBS with increasing amounts of the test compounds for 40
hours, with OSV as a positive control. Cytotoxicity was
assessed with the CellTiter-Glo assay as described above.
The cells were treated with dierent concentrations of sul-
foraphane from the standard and extract samples after 40
hours. The cytotoxicity was measured by the neutral red
uptake assay (Tecan Innite M2000 PRO; Tecan Group Ltd).
The cytotoxicity was computed by comparisons to normal
cells of wells containing compounds with wells containing
Data Analysis
All statistical analyses were performed by using SPSS 12.0.
The results are expressed as the means ± standard deviation
(SD) from experiments performed in triplicate. The statisti-
cal signicance between 2 groups was analyzed by Student’s
t test, and one-way ANOVA with Duncan multiple compari-
sons was used in the present study. A P value of <0.05 was
regarded as statistically signicant.
Declaration of Conflicting Interests
The author(s) declared no potential conicts of interest with respect
to the research, authorship, and/or publication of this article.
The author(s) disclosed receipt of the following nancial support
for the research, authorship, and/or publication of this article:
The present study was funded by grants from the National Key
Research and Development Program of China (2017YFD0101805),
the National Nature Science Foundation (31501761), the China
Agriculture Research System (CARS-23-A8), and the Science
and Technology Innovation Program of the Chinese Academy of
Agricultural Sciences (CAAS-ASTIP-IVFCAAS).
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... italica; broccoli) using a citrate/phosphate buffer (at pH 6.5) led to complete conversion into SFN [89]. Furthermore, in studies conducted by other groups, different species of Brassica oleracea were utilised (as a source of glucoraphanin) in order to examine the effect of different pH values (e.g., 5.0, 5.5, 6.0, 6.5, 7.0, 7.5, and 8.0) into the bio-production of SFN [88][89][90]. The outcome of these studies demonstrated that although hydrolysis of glucoraphanin was achieved, under all pH values, the content of SFN varied as it was pH-dependent [88,90,91]. ...
... Furthermore, in studies conducted by other groups, different species of Brassica oleracea were utilised (as a source of glucoraphanin) in order to examine the effect of different pH values (e.g., 5.0, 5.5, 6.0, 6.5, 7.0, 7.5, and 8.0) into the bio-production of SFN [88][89][90]. The outcome of these studies demonstrated that although hydrolysis of glucoraphanin was achieved, under all pH values, the content of SFN varied as it was pH-dependent [88,90,91]. Finally, the authors have concluded that pH 7.0 is the optimum condition for myrosinase activity during hydrolysis of sinigrin from Armoracia rusticana (horseradish), a finding consistent with the relevant bibliography [92,93]. ...
Full-text available
Isothiocyanates are biologically active secondary metabolites liberated via enzymatic hydrolysis of their sulfur enriched precursors, glucosinolates, upon tissue plant disruption. The importance of this class of compounds lies in their capacity to induce anti-cancer, anti-microbial, anti-inflammatory, neuroprotective, and other bioactive properties. As such, their isolation from natural sources is of utmost importance. In this review article, an extensive examination of the various parameters (hydrolysis, extraction, and quantification) affecting the isolation of isothiocyanates from naturally-derived sources is presented. Overall, the effective isolation/extraction and quantification of isothiocyanate is strongly associated with their chemical and physicochemical properties, such as polarity-solubility as well as thermal and acidic stability. Furthermore, the successful activation of myrosinase appears to be a major factor affecting the conversion of glucosinolates into active isothiocyanates.
... However, this treatment did not decrease lung cytokine levels, but rather, increased in IFN-g, IL-1b, and TNF-a compared to the control group (76). Importantly, the prophylactic treatment of mice with glucan-SFN also reduced viral titers in the lung (76), but this is possibly due to SFN acting on Nrf2 and blocking viral entry into and replication in epithelial cells (77,78). To our knowledge, SFN has not been tested therapeutically. ...
... In the case of SFN, the mice were given the compound for 2 weeks, well before infection (76). SFN treatment activated host Nrf2, which blocked viral entry in epithelial cells resulting in an antiviral response (77,78). Therapeutic treatment after influenza infection with EGCG, curcumin, and LSW (39,64,69) also showed protection against influenza infection, but whether these responses were the result of direct antiviral activity or altered TLR4 expression and signaling will require further investigation. ...
Respiratory viral infections have been a long-standing global burden ranging from seasonal recurrences to the unexpected pandemics. The yearly hospitalizations from seasonal viruses such as influenza can fluctuate greatly depending on the circulating strain(s) and the congruency with the predicted strains used for the yearly vaccine formulation, which often are not predicted accurately. While antiviral agents are available against influenza, efficacy is limited due to a temporal disconnect between the time of infection and symptom development and viral resistance. Uncontrolled, influenza infections can lead to a severe inflammatory response initiated by pathogen-associated molecular patterns (PAMPs) or host-derived danger-associated molecular patterns (DAMPs) that ultimately signal through pattern recognition receptors (PRRs). Overall, these pathogen-host interactions result in a local cytokine storm leading to acute lung injury (ALI) or the more severe acute respiratory distress syndrome (ARDS) with concomitant systemic involvement and more severe, life threatening consequences. In addition to traditional antiviral treatments, blocking the host’s innate immune response may provide a more viable approach to combat these infectious pathogens. The SARS-CoV-2 pandemic illustrates a critical need for novel treatments to counteract the ALI and ARDS that has caused the deaths of millions worldwide. This review will examine how antagonizing TLR4 signaling has been effective experimentally in ameliorating ALI and lethal infection in challenge models triggered not only by influenza, but also by other ALI-inducing viruses.
... Then, the frozen samples were dried in a lyophilizer (BETA 2-8 LD plus, Christ). The dried samples were powdered using an IKA-A10 (IKA-Werke GmbH & Co. KG) mill, and the fine powder was used for SF extraction and quantitative analysis by RP-HPLC according to methods described in our previous reports 3,22,56 . According to mixed major gene plus polygene inheritance analysis, genetic analysis of sulforaphane content in the DH population and parental lines was performed following our previous report 57 . ...
Full-text available
Glucoraphanin is a major secondary metabolite found in Brassicaceae vegetables, especially broccoli, and its degradation product sulforaphane plays an essential role in anticancer. The fine mapping of sulforaphane metabolism quantitative trait loci (QTLs) in broccoli florets is necessary for future marker-assisted selection strategies. In this study, we utilized a doubled haploid population consisting of 176 lines derived from two inbred lines (86,101 and 90,196) with significant differences in sulforaphane content, coupled with extensive genotypic and phenotypic data from two independent environments. A linkage map consisting of 438 simple sequence repeats markers was constructed, covering a length of 1168.26 cM. A total of 18 QTLs for sulforaphane metabolism in broccoli florets were detected, 10 were detected in 2017, and the other 8 were detected in 2018. The LOD values of all QTLs ranged from 3.06 to 14.47, explaining 1.74-7.03% of the biochemical variation between two years. Finally, 6 QTLs (qSF-C3-1, qSF-C3-2, qSF-C3-3, qSF-C3-5, qSF-C3-6 and qSF-C7) were stably detected in more than one environment, each accounting for 4.54-7.03% of the phenotypic variation explained (PVE) and a total of 30.88-34.86% of PVE. Our study provides new insights into sulforaphane metabolism in broccoli florets and marker-assisted selection breeding in Brassica oleracea crops.
... The authors conclude that nasal influenza infection may induce complex changes in peripheral blood NK cell activation, and that BSH (rich in SFN) effect may be important for enhanced antiviral defense responses. Li et al. [51] studied the effect of SFN on influenza A virus replication in Madin-Darby canine kidney cells. They detected an increased accumulation of Nrf2 factor triggered by SFN, resulting in a decrease of virus replication. ...
Full-text available
Brassicaceae are an outstanding source of bioactive compounds such as ascorbic acid, polyphenols, essential minerals, isothiocyanates and their precursors, glucosinolates (GSL). Recently, GSL gained great attention because of the health promoting properties of their hydrolysis products: isothiocyanates. Among them, sulforaphane (SFN) became the most attractive one owing to its remarkable health-promoting properties. SFN may prevent different types of cancer and has the ability to improve hypertensive states, to prevent type 2 diabetes–induced cardiomyopathy, and to protect against gastric ulcer. SFN may also help in schizophrenia treatment, and recently it was proposed that SFN has potential to help those who struggle with obesity. The mechanism underlying the health-promoting effect of SFN relates to its indirect action at cellular level by inducing antioxidant and Phase II detoxifying enzymes through the activation of transcription nuclear factor (erythroid-derived 2)-like (Nrf2). The effect of SFN on immune response is generating scientific interest, because of its bioavailability, which is much higher than other phytochemicals, and its capacity to induce Nrf2 target genes. Clinical trials suggest that sulforaphane produces favorable results in cases where pharmaceutical products fail. This article provides a revision about the relationship between sulforaphane and immune response in different diseases. Special attention is given to clinical trials related with immune system disorders.
... italica) is a variant of Brassica oleracea. Broccoli is a highly popular vegetable in the international market, and it is rich in nutrition, especially the anticancer and antivirus active ingredient sulforaphane (Arnison et al., 1990;Li et al., 2019aLi et al., , 2019b. ...
The planting density of broccoli can directly affect the yield and overall health of plants. Nonetheless, studies on the genetic base of planting density in broccoli are rather limited. In this study, the important planting density-dependent factors of broccoli, plant height (PH), maximum outer petiole length (PL) and leaf width (LW), were investigated during 2017 and 2018. In this study, mapping of QTL for PH, PL and LW based on a DH population in broccoli, and as well as the interaction between QTLs and the environment. The DH population was constructed with 176 genotypes obtained from F 1 hybrid produced by crossing two different inbred lines 86101 (P 1) and 90196 (P 2). In our study, a linkage group including a total of 438 SSR markers were constructed covering a length of 1168.26 cM using QTL IciMapping 4.0 software. Finally, there were mainly four QTLs (phc1, phc2, phc4− 2, phc4− 3), one QTL (plc6− 2), and two QTLs (lwc1, lwc3− 1) corresponding for PH, PL and LW recurred during the two years. In three environments, inclusive composite interval mapping (ICIM) analysis showed that there was a major QTL for PH at 7.20 cM on chromosome 1 with a high explanatory contribution rate of 20.05 %. The QTL at the 11.10 cM position of chromosome 6 was located for the PL with a high explanatory contribution rate of 20.02 %. The QTL at the 147.00 cM position of chromosome 3 was located on LW with a high explanatory contribution rate of 19.97 %. Interestingly, we found an overlap region (pplc4) in the chromosome 4 containing three QLTs for traits PH, PL, and LW (phc4− 2, plc4− 3, and lwc4− 2). According to this study, the possible positions of candidate genes were screened to provide a basis for further locating and cloning genes for plant height, maximum outer petiole and leaf width, which would be beneficial to breeding of broccoli and the Brassica crops, especially in high planting density.
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Emerging viruses are known to pose a threat to humans in the world. COVID-19, a newly emerging viral respiratory disease, can spread quickly from people to people via respiratory droplets, cough, sneeze, or exhale. Up to now, there are no specific therapies found for the treatment of COVID-19. In this sense, the rising demand for effective antiviral drugs is stressed. The main goal of the present study is to cover the current literature about bioactive compounds (e.g., polyphenols, glucosinolates, carotenoids, minerals, vitamins, oligosaccharides, bioactive peptides, essential oils, and probiotics) with potential efficiency against COVID-19, showing antiviral activities via the inhibition of coronavirus entry into the host cell, coronavirus enzymes, as well as the virus replication in human cells. In turn, these compounds can boost the immune system, helping fight against COVID-19. Overall, it can be concluded that bioactives and the functional foods containing these compounds can be natural alternatives for boosting the immune system and defeating coronavirus.
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The current emergence of the COVID-19 virus has originated a global disaster due to the unavailability of any type of vaccine or drug which can be effective and determined to combat it. Naturally, various probabilities (such as herbal remedies with known medical importance) have been investigated by scientists. Research of systematic scientific (starting from silico research) of herbal medicine in specific and any type of drug in common is now possible that is building blocks (proteins) of COVID-19 which are already being identified. Mpro or 3CLpro is the main protease of the COVID-19 virus which have an important CoV enzyme and an attractive drug target because it plays a very important role in the viral replication and transcription. By using molecular docking scientist's aims to explore bioactive chemicals which are found in medicinal plants such as potential COVID-19 Mpro inhibitors. Molecular docking was performed by Autodock 4.2, and the Lamarckian Genetic Algorithm, for analyzing the possibility of a dock. Although, further research is needed here to investigate their uses of potential drug. And the search for new drugs, especially through the use of antiviral peptides, is a very possible and protein-protein docking were performed using silico methods to identify, test, and evaluate cell interactions and interactions of dermaseptin peptide molecules produced by Phyllomedusa frogs against SARS-CoV-2 spike protein macromolecule, with its effect on the surface attachment of the ACE-2 (Angiotensin Converting Enzyme-2) receptor. Therefore, it is hoped that the dermaseptin-S9 peptide molecule may continue to be studied in the formulation of antibodies to anti-virus peptides that will be able to control COVID-19 infectious diseases.
Throughout history, disease outbreaks have worked havoc upon humanity, sometimes reorienting the history and at times, signaling the end of entire civilizations and the modern pandemic that the world is dealing with, is COVID-19 or SARS-CoV-2. A healthy immunity could be an ideal gear for resisting COVID-19 for neither medicines nor vaccines have been ascertained till date. In view of the present scenario, there is a demanding necessity to analyze innovative and valid techniques for forestalling and cure of COVID-19 by re-evaluating the structure of the natural compounds for drug designing. The Ayurveda has come forward by prescribing a lot of medicinal herbs for combating this dreaded disease. We have searched from sources in Pubmed and Google Scholar and found 1509 items. The search criteria were limited to the effect of phytochemicals in certain immunomodulatory aspects of viral infection. The original research papers related to the works on phytochemicals in the down regulation of NF-kB, activation of NK and CD8+ cells, inhibition of inflammatory cytokine release and ROS scavenging were included in our study. Here, we try to focus on the immunoregulatory cells which have a vital aspect in COVID-19 and highlight the potential effects of the restorative use of phytochemicals as drugs or dietary supplements. Supplementary information: The online version contains supplementary material available at 10.1007/s13337-021-00706-2.
Reactive oxygen species (ROS) are by-products of cellular metabolism and can be either beneficial, at low levels, or deleterious, at high levels, to the cell. It is known that several viral infections can increase oxidative stress, which is mainly facilitated by viral-induced imbalances in the antioxidant defence mechanisms of the cell. While the exact role of ROS in certain viral infections (adenovirus and dengue virus) remains unknown, other viruses can use ROS for enhancement of pathogenesis (SARS coronavirus and rabies virus) or replication (rhinovirus, West Nile virus and vesicular stomatitis virus) or both (hepatitis C virus, human immunodeficiency virus and influenza virus). While several viral proteins (mainly for hepatitis C and human immunodeficiency virus) have been identified to play a role in ROS formation, most mediators of viral ROS modulation are yet to be elucidated. Treatment of viral infections, including hepatitis C virus, human immunodeficiency virus and influenza virus, with ROS inhibitors has shown a decrease in both pathogenesis and viral replication both in vitro and in animal models. Clinical studies indicating the potential for targeting ROS-producing pathways as possible broad-spectrum antiviral targets should be evaluated in randomized controlled trials.
Human macrophages are protected by intrinsic antiviral defenses that provide moderate protection against HIV-1 infection. Macrophages that do become infected can serve as long-lived reservoirs, to disseminate HIV-1 to CD4⁺ T cells. Infection of macrophages with HIV-1 and HIV-2 is inhibited by constitutive mobilization of antioxidant response master transcription regulator Nrf2. The downstream mediator of this restriction was not identified. Among the tens of genes controlled directly by Nrf2 in macrophages, we found that xCT/SLC7A11, a 12-transmembrane, cystine-glutamate antiporter promotes antiretroviral activity. We show here that depletion of xCT mRNA increases HIV-1 infection. Reconstitution of xCT knock out cells with wild-type xCT but not a transport-deficient mutant restores anti-HIV-1 activity. Pharmacological inhibitors of xCT amino acid transport also increase infection. The block is independent of known restriction factors and acts against HIV-1 and HIV-2. Like the block triggered through Nrf2, xCT function impedes infection immediately before 2-LTR circle formation.
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We compared the characteristics of cases of highly pathogenic avian influenza (HPAI) and low pathogenic avian influenza (LPAI) A(H7N9) virus infections in China. HPAI A(H7N9) case-patients were more likely to have had exposure to sick and dead poultry in rural areas and were hospitalized earlier than were LPAI A(H7N9) case-patients. © 2017, Centers for Disease Control and Prevention (CDC). All rights reserved.
Dendrobine, a major component of Dendrobium nobile, increasingly draws attention to its wide applications in health care. Here we explore potential effect of dendrobine against influenza A virus and elucidate the underlying mechanism. Our results indicated that dendrobine possessed antiviral activity against influenza A viruses, including A/FM-1/1/47 (H1N1), A/Puerto Rico/8/34 H274Y (H1N1) and A/Aichi/2/68 (H3N2) with IC50 value of 3.39±0.32, 2.16±0.91, 5.32±1.68 μg/mL, respectively. Mechanism studies revealed that dendrobine inhibited early steps in the viral replication cycle. Notably, dendrobine could bind to the highly conserved region of viral nucleoprotein (NP), subsequently restraining nuclear export of viral NP and its oligomerization. In conclusion, dendrobine shows potential to be developed as a promising agent to treat influenza virus infection. More importantly, the results provide invaluable information for the fully application of Traditional Chinese Medicine named "Shihu".
Background: Since the re-discovery of sulforaphane in 1992 and the recognition of the bioactivity of this phytochemical, many studies have examined its mode of action in cells, animals and humans. Broccoli, especially as young sprouts, is a rich source of sulforaphane and broccoli-based preparations are now used in clinical studies probing efficacy in health preservation and disease mitigation. Many putative cellular targets are affected by sulforaphane although only one, KEAP1-NRF2 signaling, can be considered a validated target at this time. The transcription factor NRF2 is a master regulator of cell survival responses to endogenous and exogenous stressors. Scope and approach: This review summarizes the chemical biology of sulforaphane as an inducer of NRF2 signaling and efficacy as an inhibitor of carcinogenesis. It also provides a summary of the current findings from clinical trials using a suite of broccoli sprout preparations on a series of short-term endpoints reflecting a diversity of molecular actions. Key findings and conclusions: Sulforaphane, as a pure chemical, protects against chemical-induced skin, oral, stomach, colon, lung and bladder carcinogenesis and in genetic models of colon and prostate carcinogenesis. In many of these settings the antitumorigenic efficacy of sulforaphane is dampened in Nrf2-disrupted animals. Broccoli preparations rich in glucoraphanin or sulforaphane exert demonstrable pharmacodynamic action in over a score of clinical trials. Measures of NRF2 pathway response and function are serving as guideposts for the optimization of dose, schedule and formulation as clinical trials with broccoli-based preparations become more commonplace and more rigorous in design and implementation.
Activation of nuclear factor erythroid 2-related factor 2 (Nrf2, a transcription factor) and/or inhibition of mammalian target of rapamycin (mTOR) are implicated in the suppression of vascular smooth muscle cell (VSMC) proliferation. The present study has examined the likely regulatory effects of sulforaphane (SFN, an antioxidant) on Nrf2 activation and platelet-derived growth factor (PDGF)-induced mTOR signaling in VSMCs. Using human aortic VSMCs, nuclear extraction and siRNA-mediated downregulation studies were performed to determine the role of Nrf2 on SFN regulation of PDGF-induced proliferative signaling. Immunoprecipitation and/or immunoblot studies were carried out to determine how SFN regulates PDGF-induced mTOR/p70S6 K/S6 versus ERK and Akt signaling. Immunohistochemical analysis was performed to determine SFN regulation of S6 phosphorylation in the injured mouse femoral artery. SFN (5 μM) inhibits PDGF-induced activation of mTOR without affecting mTOR association with raptor in VSMCs. While SFN inhibits PDGF-induced phosphorylation of p70S6K and 4E-BP1 (downstream targets of mTOR), it does not affect ERK or Akt phosphorylation. In addition, SFN diminishes exaggerated phosphorylation of S6 ribosomal protein (a downstream target of p70S6K) in VSMCs in vitro and in the neointimal layer of injured artery in vivo. Although SFN promotes Nrf2 accumulation to upregulate cytoprotective genes (e.g., heme oxygenase-1 and thioredoxin-1), downregulation of endogenous Nrf2 by target-specific siRNA reveals an Nrf2-independent effect for SFN-mediated inhibition of mTOR/p70S6K/S6 signaling and suppression of VSMC proliferation. Strategies that utilize local delivery of SFN at the lesion site may limit restenosis after angioplasty by targeting mTOR/p70S6K/S6 axis in VSMCs independent of Nrf2 activation.
Influenza is a very common contagious disease that carries significant morbidity and mortality. Treatment with antiviral drugs is available, which if administered early, can reduce the risk of severe complications. However, many virus types develop resistance to those drugs, leading to a notable loss of efficacy. There has been great interest in the development of new drugs to combat this disease. A wide range of drugs has shown anti-influenza activity, but they are not yet available for use in the clinic. Many of these target viral components, which others are aimed at elements in the host cell which participate in the viral cycle. Modulating host components is a strategy which minimizes the development of resistance, since host components are not subject to the genetic variability of the virus. The main disadvantage is the risk of treatment-related side effects. The aim of this review is to describe the main pharmacological agents currently available and new drugs in the pipeline with potential benefit in the treatment of influenza.
Scope: The isothiocyanate sulforaphane (SF) from broccoli, is one of the most potent known inducers of the cytoprotective phase 2 response. Its role in a host of biochemical pathways make it a major component of plant-based protective strategies for enhancing healthspan. Many nutritional supplements are now marketed that purport to contain SF, which in plants exists as a stable precursor, a thioglucoside hydroxysulfate. However, SF in pure form must be stabilized for use in supplements. Methods and results: We evaluated the stability and bioavailability of two stabilized SF preparations - an α-cyclodextrin inclusion (SF-αCD), and a SF-rich, commercial nutritional supplement. SF-αCD area-under-the-curve (AUC) peak serum concentrations occurred at 2 hours, but 6 of 10 volunteers complained of mild stomach upset. After topical application it was not effective in up-regulating cytoprotective enzymes in the skin of SKH1 mice whereas pure SF was effective in doing so. Both of these "stabilized" SF preparations were as potent as pure SF in inducing the cytoprotective response in cultured cells, and they were more stable and as bioavailable. Conclusion: Our studies of a stabilized phytochemical component of foods should encourage further examination of similar products for their utility in chronic disease prevention and therapy. This article is protected by copyright. All rights reserved.
Influenza is a very common contagious disease that carries significant morbidity and mortality. Treatment with antiviral drugs is available, which if administered early, can reduce the risk of severe complications. However, many virus types develop resistance to those drugs, leading to a notable loss of efficacy. There has been great interest in the development of new drugs to combat this disease. A wide range of drugs has shown anti-influenza activity, but they are not yet available for use in the clinic. Many of these target viral components, which others are aimed at elements in the host cell which participate in the viral cycle. Modulating host components is a strategy which minimizes the development of resistance, since host components are not subject to the genetic variability of the virus. The main disadvantage is the risk of treatment-related side effects. The aim of this review is to describe the main pharmacological agents currently available and new drugs in the pipeline with potential benefit in the treatment of influenza. Copyright © 2016 SEPAR. Publicado por Elsevier España, S.L.U. All rights reserved.
Oxidative stress is known to play an important role in oral cancer development. In this study we aimed to examine whether a chemical activator of NRF2, sulforaphane (SFN), may have chemopreventive effects on oxidative stress-associated oral carcinogenesis. We first showed that Nrf2 activation and oxidative damage were commonly seen in human samples of oral leukoplakia. With gene microarray and immunostaining, we found 4-nitroquinoline 1-oxide (4NQO) in drink activated the Nrf2 pathway and produced oxidative damage in mouse tongue. Meanwhile whole exome sequencing of mouse tongue identified mutations consistent with 4NQO's mutagenic profile. Using cultured human oral keratinocytes and 4NQO-treated mouse tongue, we found that SFN pre-treatment activated the NRF2 pathway and inhibited oxidative damage both in vitro and in vivo. On the contrary, a structural analogue of SFN without the isothiocyanate moiety did not have such effects. In a long-term chemoprevention study using wild-type and Nrf2-/- mice, we showed that topical application of SFN activated the NRF2 pathway, inhibited oxidative damage, and prevented 4NQO-induced oral carcinogenesis in an Nrf2-dependent manner. Our data clearly demonstrate that SFN has chemopreventive effects on oxidative stress-associated oral carcinogenesis, and such effects depend on Nrf2 and the isothiocyanate moiety.