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ANTIMICROBIAL EFFICACY OF OREGANO EXTRACTS
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... Similarly, the antibacterial activities of water, ethanolic and methanolic extracts of EOs obtained from O. vulgare plants against Gram-positive and Gram-negative microorganisms were investigated and the results were compared with those of control compounds such as Vancomycin, Erythromycin, Cloxacillin, Ciprofloxacin and Streptomycin. It was determined that all extracts exhibit pronounced antibacterial effects and there are significant differences between the effects of extracts and control drugs [66]. ...
... Similarly, the antibacterial activities of water, ethanolic and methanolic extracts of EOs obtained from O. vulgare plants against Gram-positive and Gram-negative microorganisms were investigated and the results were compared with those of control compounds such as Vancomycin, Erythromycin, Cloxacillin, Ciprofloxacin and Streptomycin. It was determined that all extracts exhibit pronounced antibacterial effects and there are significant differences between the effects of extracts and control drugs [66]. ...
... Similarly, the antibacterial activities of water, ethanolic and methanolic extracts of EOs obtained from O. vulgare plants against Gram-positive and Gram-negative microorganisms were investigated and the results were compared with those of control compounds such as Vancomycin, Erythromycin, Cloxacillin, Ciprofloxacin and Streptomycin. It was determined that all extracts exhibit pronounced antibacterial effects and there are significant differences between the effects of extracts and control drugs [66]. ...
The effects of humic acid on plant yield, essential oil content, the composition of essential oil and the antimicrobial activity of Origanum vulgare L. subsp. hirtum (Link.) (cv. Tinmaz) cultivated in 2017 and 2018 under Eskisehir ecological conditions were evaluated. Three humic acid (HA) doses in response to 50.0 L ha−1 (HA 50), 30.0 L ha−1 (HA 30) and 0.0 L ha−1 (HA 0, as control) were applied to soil at the vegetative stage and beginning of the blooming stage of the plant throughout each harvest in both years. Essential oil composition was determined using GC-FID/GC-MS. The antibacterial and antifungal activity were determined by the well-diffusion method. Fresh herb yield, dry herb yield and dry leaf yield were highest at HA 50 both years, although essential oil content increased in 2017 at both HA 50 and HA 30, but was greatest at HA 50 in the second year. The essential oil content differences between the control dose and 50.0 L HA ha−1 were 0.46% and 0.42% in 2017 and 2018, respectively. The antimicrobial activity of the essential oil samples against two bacteria species (Enterococcus faecalis and Staphylococcus aureus and two yeast species (Candida albicans and Candida parapisilosis) was generally higher than that of the control drugs, and the activity increased with increasing HA doses. Analysis of the essential oil components showed that the carvacrol and γ-Terpinene ratios generally increased as the HA doses increased to 50.0 L HA ha−1. Soil HA applications could be recommended for higher quality, plant yield and antimicrobial activity of Origanum vulgare L. subsp. hirtum.
... Although both the ethanolic extract and the essential oil exhibit antibacterial properties, the highest activity has been reported for the essential oil. The activity observed with the essential oil could be related to the presence of carvacrol and thymol as major compounds, while the oregano extract contains a larger variety of compounds (limonene, gamma-cariofilene, rhocymenene, canfor, linalool, alpha-pinene, carvacrol, and thymol) [49,50]. ...
Adherence of bacteria to the human intestinal mucosa can facilitate their internalization and the development of pathological processes. Escherichia coli O104:H4 is considered a hybrid strain (enteroaggregative hemorrhagic E. coli [EAHEC]), sharing virulence factors found in enterohemorrhagic (EHEC), and enteroaggregative (EAEC) E. coli pathotypes. The objective of this study was to analyze the effects of natural and synthetic antimicrobials (carvacrol, oregano extract, brazilin, palo de Brasil extract, and rifaximin) on the adherence of EHEC O157:H7, EAEC 042, and EAHEC O104:H4 to HEp-2 cells and to assess the expression of various genes involved in this process. Two concentrations of each antimicrobial that did not affect (p≤0.05) bacterial viability or damage the bacterial membrane integrity were used. Assays were conducted to determine whether the antimicrobials alter adhesion by affecting bacteria and/or alter adhesion by affecting the HEp-2 cells, whether the antimicrobials could detach bacteria previously adhered to HEp-2 cells, and whether the antimicrobials could modify the adherence ability exhibited by the bacteria for several cycles of adhesion assays. Giemsa stain and qPCR were used to assess the adhesion pattern and gene expression, respectively. The results showed that the antimicrobials affected the adherence abilities of the bacteria, with carvacrol, oregano extract, and rifaximin reducing up to 65% (p≤0.05) of E. coli adhered to HEp-2 cells. Carvacrol (10 mg/ml) was the most active compound against EHAEC O104:H4, even altering its aggregative adhesion pattern. There were changes in the expression of adhesion-related genes (aggR, pic, aap, aggA, and eae) in the bacteria and oxidative stress-related genes (SOD1, SOD2, CAT, and GPx) in the HEp-2 cells. In general, we demonstrated that carvacrol, oregano extract, and rifaximin at sub-minimal bactericidal concentrations interfere with target sites in E. coli, reducing the adhesion efficiency.
... These extracts show various medicinal properties especially antimicrobial property. The emergence of multiple drug resistant infectious bacteria, high cost of synthetic compounds as well as undesirable side effects of certain drugs insist on pharmaceutical companies to look for new therapeutic agents from other alternative sources including medicinal plants [5]. Over the past several years, many researchers have been reviewed the antimicrobial activities which had been discovered by intensive efforts. ...
To investigate the two different germplasm of Mucuna seeds were collected from agro geographical regions was evaluated for its antibacterial activities. Antibacterial activity of the seed extracts was studied against the fish pathogens of Aeromonas hydrophila, Pseudomonas fluorescens, Vibrio cholera and Klebsiella pneumonia using agar well diffusion method. Results showed that methanol and ethanol extracts showed more potent antibacterial activity than other solvent extracts. The results were expressed as mean ± SD. The results obtained in the study shows that velvet bean black seed extract has more antibacterial activity against fish pathogens. The antibacterial activity of all the Mucuna seed extracts are comparable ad their potential as alternative in the treatment of infectious by these microorganisms was present in the fish. Susceptibility testing is conducted on isolates using drug selected on the basis of their importance to human medicine and use I fish production.
... These extracts show various medicinal properties especially antimicrobial property. The emergence of multiple drug resistant infectious bacteria, high cost of synthetic compounds as well as undesirable side effects of certain drugs insist on pharmaceutical companies to look for new therapeutic agents from other alternative sources including medicinal plants [5]. Over the past several years, many researchers have been reviewed the antimicrobial activities which had been discovered by intensive efforts. ...
... These extracts show various medicinal properties especially antimicrobial property. The emergence of multiple drug resistant infectious bacteria, high cost of synthetic compounds as well as undesirable side effects of certain drugs insist on pharmaceutical companies to look for new therapeutic agents from other alternative sources including medicinal plants [5]. Over the past several years, many researchers have been reviewed the antimicrobial activities which had been discovered by intensive efforts. ...
To investigate the two different germplasm of Mucuna seeds were collected from agro geographical regions was evaluated for its antibacterial activities. Antibacterial activity of the seed extracts was studied against the fish pathogens of Aeromonas hydrophila, Pseudomonas fluorescens, Vibrio cholera and Klebsiella pneumonia using agar well diffusion method. Results showed that methanol and ethanol extracts showed more potent antibacterial activity than other solvent extracts. The results were expressed as mean ± SD. The results obtained in the study shows that velvet bean black seed extract has more antibacterial activity against fish pathogens. The antibacterial activity of all the Mucuna seed extracts are comparable ad their potential as alternative in the treatment of infectious by these microorganisms was present in the fish. Susceptibility testing is conducted on isolates using drug selected on the basis of their importance to human medicine and use I fish production.
The problem of antibiotic resistance has become increased in recent years. The present study investigated the antibacterial activity of aqueous extract of Rhus coriaria against Staphylococcus aureus in vitro and in vivo. The results showed that S. aureus was multi-drug resistant where it was resistant to nine antimicrobials (AMC, CFM, CTR, DA, OB, MY, ME, NIT, and V) among seventeen used antimicrobials. The aqueous extract of R. coriaria showed a strong effect in inhibition the growth of tested isolate in vitro and in vivo. The MIC was determined where it was 0.025% and was used as curing agent for curing bacterial plasmids. The phytochemical screening showed that R. coriaria contains tannin, phenol, anthraquinon and saponin.
In this study, the antibacterial and antifungal activities of Rhus coriaria L. (Anacardiaceae), Stachys annua (L.) subsp. annua Ic., Stachys pumilia Banks & Sol., Laurus nobilis L. (Lauraceae), Allium neapolitanum Lyr. (Liliaceae), Salvia viridis L. (Lamiaceae), and Nicotina rustica (Solanaceae) species were investigated. The microbial effects of these plants were tested by a disk diffusion method using Bacillus megaterium DSM 32, Bacillus brevis FMC 3, Bacillus subtilis IMG 22, Bacillus cereus FMC 19, Escherichia coli DM, Enterobacter aerogenes CCM 2531, Pseudomonas aeruginosa DSM 50071, Staphylococcus aureus Cowan 1, Listeria monocytogenes Scott A and Micrococcus luteus LA 2971, Candida tropicalis and Candida albicans CCM 314. The results showed that the fruit extract of R. coriaria had the strongest antimicrobial effect with an inhibition zone of 35–51 mm against all the bacteria used, while S. viridis demonstrated the weakest antibacterial effect, inhibiting only the development of S. aureus, with an inhibition zone of 11 mm. A. neapolitanum, L. nobilis and N. rustica extracts were effective only with some yeasts. The growth of S. aureus was inhibited by all the plant extracts except for S. annua subsp. annua, having an inhibition zone ranging from 7–8 mm. With the exception of B. subtilis IMG 22, L. monocytogenes Scott A and M. luteus LA 2971, the growth of the other bacteria was inhibited by all the extracts. Except for the fruit extracts of R. coriaria and A. neapolitanum, all additional extracts of generated inhibition zones smaller than those generated by several reference antibiotics.
Oregano is used worldwide both as spice and crude drug, which is mainly provided by species of Origanum genus. The quality of the product is usually determined by chemical analysis, whereas in food industrial applications sensory tests are also practised. The aim of the present study was a comparison of parallel quality investigations of oregano samples by a new and effective instrumental sensory evaluation method, the "electronic nose", and by gas-chromatographic and human sensory analysis. The GC analysis of essential oil components revealed mainly differences between plant species (Origanum vulgare subsp. hirtum and Origanum majorana). Main components of the oil of the former taxon are carvacrol and thymol, while those of marjoram are terpinene-4-ol, -terpinene and terpinolene. A wholesale oregano sample showing considerable divergence from the other ones with respect to ratios of carvacrol, -caryophyllene -cubebene and thymol. It was assumed not to belong to ssp. hirtum The electronic nose analysis, evaluated by PCA, proved to be an appropriate, rapid, non-destructive, reagent-less method for the reliable separation of all of the oregano samples based on their complex aroma features. Assumptions could be made about correlations between separation of samples by the instrumental sensors and proportions of terpenoid compounds of the oil established by GC in some cases only. The varying essential oil content of the samples did not influence the success of instrumental evaluation. The instrumental and human sensory analysis showed similar results: varieties of O. majorana could be well distinguished on the basis of their complex aroma, while their gas-chromatograms did not show characteristic differences. The results call the attention that quality evaluation of drug items of aromatic plants should be oriented in different directions, considering the current utilisation area of the items.
The oregano spice includes various plant species. The most common are the genus Origanum, native of Europe, and the Lippia, native of Mexico. Among the species of Origanum. their most important components are the limonene, gamma-cariofilene, rho-cymenene, canfor, linalol, alpha-pinene, carvacrol and thymol. In the genus Lippia, the same compounds can be found. The oregano composition depends on the specie, climate, altitude, time of recollection and the stage of growth. Some of the properties of this plant's extracts are being currently studied due to the growing interest for substituting synthetic additives commonly found in foods. Oregano has a good antioxidant capacity and also presents antimicrobial activity against pathogenic microorganisms like Salmonella typhimurium, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, among others. These are all characteristics of interest for the food industry because they may enhance the safety and stability of foods. There are also some reports regarding the antimutagenic and anticarcinogenic effect of oregano; representing an alternative for the potential treatment and/or prevention of certain chronic ailments, like cancer.
Many herbs are known as excellent sources of natural antioxidants, and consumption of fresh herbs in the diet may therefore contribute to the daily antioxidant intake. The present study was performed in order to quantify flavonoids in commonly eaten fresh herbs. Fifteen fresh herbs (basil, chives, coriander, cress, dill, lemon balm, lovage, oregano, parsley, rosemary, sage, spearmint, tarragon, thyme, and watercress) were analysed by HPLC and mass spectrometry. Five major flavonoid aglycones were detected and quantified by HPLC after acid hydrolysis: apigenin, isorhamnetin, kaempferol, luteolin, and quercetin. The highest levels of flavonoids were found in parsley (510–630 mg apigenin /100 g), lovage (170 mg quercetin/100g), mint (18–100 mg apigenin/100 g), and dill (48–110 mg quercetin/100 g). Mass spectrometric detection, using atmospheric pressure chemical ionisation (APCI), was used to verify the presence of flavonoids in the hydrolysed extracts of herbs. Some traditional Danish dishes contain herbs, particularly parsley, dill, cress and chives, and the contribution to the flavonoid intake by consumption of these dishes was calculated.
The present investigation is focused on antibacterial potential of infusion, decoction and essential oil of oregano (Origanum vulgare) against 111 Gram-positive bacterial isolates belonging to 23 different species related to 3 genera. Infusion and essential oil exhibited antibacterial activity against Staphylococcus saprophyticus, S. aureus, Micrococcus roseus, M. kristinae, M. nishinomiyaensis, M. lylae, M. luteus, M. sedentarius, M. varians, Bacillus megaterium, B. thuringiensis, B. alvei, B. circulans, B. brevis, B. coagulans, B. pumilus, B. laterosporus, B. polymyxa, B. macerans, B. subtilis, B. firmus, B. cereus and B. lichiniformis. The infusion exhibited maximum activity against B. laterosporus (17.5 mm mean zone of inhibition+/-1.5 Standard deviation) followed by B. polymyxa (17.0 mm+/-2.0 SD) and essential oil of oregano exhibited maximum activity against S. saprophyticus (16.8 mm+/-1.8 SD) followed by B. circulans (14.5 mm+/-0.5 SD). While all these tested isolates were found resistant to decoction of oregano.
Ethanolic extracts of 16 Turkish plant species used in folk medicine were investigated for their antimicrobial activities against nine bacterial species ( Escherichia coli , Staphylococcus aureus , Klebsiella pneumoniae , Pseudomonas aeruginosa , Proteus vulgaris , Bacillus cereus , Mycobacterium smegmatis , Listeria monocytogenes and Micrococcus luteus ) and three yeasts ( Candida albicans , Kluyveromyces fragilis and Rhodotorula rubra ) using the disc diffusion method. Of the 16 plants tested, ten showed antimicrobial activity. Each plant species has unique against different microorganisms. The most active antimicrobial plant against bacteria and yeasts was Myrtus communis . It is conclude the plants studied may be sources of antimicrobial agents.
Tannins (commonly referred to as tannic acid) are water-soluble polyphenols that are present in many plant foods. They have been reported to be responsible for decreases in feed intake, growth rate, feed efficiency, net metabolizable energy, and protein digestibility in experimental animals. Therefore, foods rich in tannins are considered to be of low nutritional value. However, recent findings indicate that the major effect of tannins was not due to their inhibition on food consumption or digestion but rather the decreased efficiency in converting the absorbed nutrients to new body substances. Incidences of certain cancers, such as esophageal cancer, have been reported to be related to consumption of tannins-rich foods such as betel nuts and herbal teas, suggesting that tannins might be carcinogenic. However, other reports indicated that the carcinogenic activity of tannins might be related to components associated with tannins rather than tannins themselves. Interestingly, many reports indicated negative association between tea consumption and incidences of cancers. Tea polyphenols and many tannin components were suggested to be anticarcinogenic. Many tannin molecules have also been shown to reduce the mutagenic activity of a number of mutagens. Many carcinogens and/or mutagens produce oxygen-free radicals for interaction with cellular macromolecules. The anticarcinogenic and antimutagenic potentials of tannins may be related to their antioxidative property, which is important in protecting cellular oxidative damage, including lipid peroxidation. The generation of superoxide radicals was reported to be inhibited by tannins and related compounds. The antimicrobial activities of tannins are well documented. The growth of many fungi, yeasts, bacteria, and viruses was inhibited by tannins. We have also found that tannic acid and propyl gallate, but not gallic acid, were inhibitory to foodborne bacteria, aquatic bacteria, and off-flavor-producing microorganisms. Their antimicrobial properties seemed to be associated with the hydrolysis of ester linkage between gallic acid and polyols hydrolyzed after ripening of many edible fruits. Tannins in these fruits thus serve as a natural defense mechanism against microbial infections. The antimicrobial property of tannic acid can also be used in food processing to increase the shelf-life of certain foods, such as catfish fillets. Tannins have also been reported to exert other physiological effects, such as to accelerate blood clotting, reduce blood pressure, decrease the serum lipid level, produce liver necrosis, and modulate immunoresponses. The dosage and kind of tannins are critical to these effects. The aim of this review is to summarize and analyze the vast and sometimes conflicting literature on tannins and to provide as accurately as possible the needed information for assessment of the overall effects of tannins on human health.
To analysis compositions of the volatile oil in Origanum vulgare.
GC-MS condition: using programmed temperature gas chromatography cinitial temperature was 60 degrees C, and then raising the temperature (5 degrees C/min) to 240 degrees C, mass-to-electric charge ratio was 10 to 425.
GC-MS identified 29 kinds of contents.
Thymol and carvacrol are main compositions of the volatile oil in Origanum vulgare.
- L F Nimri
- M M Meqdam
- A Alkofani
Nimri LF., Meqdam MM.and A. ALkofani (1999). Antibacterial Activity of Jordanian Medicinal Plants. Pharmaceutical Biology, 37: 196 -201.