PHYTOCHEMICAL PROFILING OF FIG FRUIT FICUS RACEMOSA EXTRACT

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PHYTOCHEMICAL PROFILING OF FIG
FRUIT FICUS RACEMOSA EXTRACT
Sivakumar.P1, Manimekalai1*.I, Sivakumari2.K, Ashok.K2
1*Department of Zoology Government Arts College for Men Krishnagiri – 635 001, India.
2Department of Zoology Presidency College Chennai – 600 005, India.
ABSTRACT
Photochemical profiling effect of fig fruit ficus racemosa have been unraveled in the
present study. Phytochemicals are organic compounds found in plants; they are not
nutrients essential for life but you should care about them because many have known or
potential health benefits. Extraction of fruit extracts of fruit with methanol, chloroform,
ethyl acetate, hexane and aqueous revealed that methanol gave yield of extract.
Phytochemicals present in the dietary fruit have anticancer properties. The phytochemical
analysis of sacred figs showed the presence of total phenolics, flavanoids, and other
secondary metabolites. However, standardization of the solvents by MTT assay showed that
only methanol extract of fruit extract of significant has profound effect on HepG2 cells. It
can be concluded that fruit extract of ficus racemosa have phytochemical potential.
Moreover, identification of active phytoconstiuents in the extract will pave a way for using
this fruit as a natural cytotoxic agent various cancers.
KEY WORDS : Ficus racemosa fruit, DAPI Method, FT-IR assay, GC-MS assay, MTT
assay, Phytoconstiuents.
INTRODUCTION
World health organization (WHO) reported that more than 11 million people are
diagnosed with cancer every year and it is estimated that there will be 16 million new cases
per year by 2020 (100) .The most common types of cancer in males are lung cancer, prostate
cancer, colorectal cancer and stomach cancer. In females, the most common types are breast
cancer, colorectal cancer, lung cancer and cervical cancer(16,66). If skin cancer other than
melanoma were included in total new cancers each year, it would account for around 40%
of cases. In children, acute lymphoblastic leukemia and brain tumors are most common
except in Africa where non-Hodgkin lymphoma occurs more often (101,102).
Phytochemicals present in the dietary fruits have anticancer properties(17,18,19,64). The
recent studies focusing on cancer have exploited the natural compounds from fruits,
Medicine has drawn much attention for the effective extraction of desired bioactive
ingredients from natural products(20). This particularly insists that fruits and vegetables,
have many phytochemicals that possess various bioactivities, including antioxidant and
anticancer properties. Fruits can add important vitamins, minerals and other bioactive
compounds in human diet(92,93).

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There are several strategies available for managing HCC. One of the important
therapeutic method is chemotherapy, limited as significant extent by fruit extraction. Most
efficient chemotherapy treatment options as HCC include surgical resection, embolization,
ablation and liver transplation(40,41) chemotherapeutic drugs prolong the survival of HCC
patients and generally well tolerated give contradictory results in terms of safety and
efficacy(25,26,27,28,45).Chemotherapy results are, as some patients undergo severe toxicities
side effects with very short survival(6,34,59,77). Development of effective cancer
chemotherapeutic agent with lesser or no side effects is required for the management of
HCC(29,30,76,103). Natural products always has been a research hotspot for the development of
new anticancer agents(2,3,4,5).
Initiation of hepatic oncogenesis, transforms hepatocytes elude various cellular
defensive activities and acquire abnormal capabilities to survive and proliferat(42,43) .The
receptor tyrosine kinases plays an important role (13,14,65) in signaling of hepatocellular
carcinoma, its development and progression.
The fig is traditionally a most important source of human food in its earliest
cultivation. Mediterranean countries ranks about seventy percent of the world fig fruit
production. The figs are important part of Mediterranean diet, related to health and
longevity (89). Antioxidant compounds such as phenolics, organic acids, vitamin E, and
carotenoids, scavenge free radicals, thus inhibiting the oxidative mechanisms to
degenerative illnesses (80).
The fig fruits vary in colour as green, yellow, brown, purple and black colour, this
colouration of fruits originate from carotenoid and anthocyanin pigments produced during
maturation (33). The fig fruits process more than 50 metabolites identified. Consumption of
these health-promoting compounds of fig may produce protection against several human
disease (37,68). Fig was also an excellent source of fiber, minerals, and Polyphenols. They are
low sodium and have no fat or cholesterol (8,43).
The material medica gives great information on the folklore practices and traditional
aspects of therapeutic natural products (7,69). Traditionally most of the treatment in india is
based on various types such as ayurveda, siddha, unani and homoeopathy. The evaluation of
drugs is based on phytochemical approaches which lead to drugs discovery and reffered to
as natural product screen (31,32). All the plants parts seed, root, bark, leaves, flowers, fruits,
etc (41,42,63) contain active components.
Ficus racemosa (moracea) is a large deciduous tree distributed all over india from
outer Himalayan ranges, Punjab, Khasia mountain Chota Nagpur, Bihar, Orissa, west
Bengal, Rajasthan, Deccan plateau and common in south india (86,71). Ficus racemosa is one
of the member of the sacred trees to be planted around the home and temples. It grows in
the evergreen forest throughout the year,Moist localities and bank of strems, deciduous

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forests, to the elevation of 1800m above sea level, often cultivated in villages for its edible
fruits (46,60,61,62,87). The ficus racemosa commonly known as gular fig in Hindi and cluster fig
in English.
The tree grows up to 18m high, leaves, ovate, lanceolate or elliptic, sub acute, entire
and petiolate leaves are shed by December and replenished by January and April, this tree
becomes bare for a short period (21). Ficus racemosa subglobose or pyriform, red when ripe
found in large clusters, on short, leafless branches and turnk (20,84). This tree do not procure
aerial roots like many of its family members, they naturally comes up in forests in
subtropical climate. They grow well in well-drained medium to heavy soils put for
successful cultivation they grow up in all kinds of soil except in water stagnant and clay soil
(3,9,10,11,12). The ficus racemosa traditionally used as medicine, the plant parts cure various
health problems and diseases (18,19).
Fig fruit have been used in the treatment of dry cough, loss of voice, diseases of
kidney and spleen (47,48,49,85). Fresh fruits contains lots of dietary fibre. It exhibits more
hypocholesterolemic activity than pure cellulose (4,5). The ficus racemosa fruit extract is
used to treat diabetes, leucoderma, aphrodisiac, menorrhagia, and also used for
inflammation of skin, wounds, lymphadenitis, in sprains and fibrositis (81,82). Its bark used as
anti-hyperglycemic agent (24,90). They extract of ficus racemosa bark, leaves and fruits are
used as antitumor, antimicrobial and anticancer (51,52).
In india, the most important species of ficus groups are ficus infectoria, ficus
religiosa, ficus carica and etc (99). The ficus infectora is a common name of white fig, this
traditionally found in Bangladesh, Nepal, Pakistan, Sri Lanka, South West China, and
Indochina (53,96) have been another review emphasizes as traditional used and clinical
potential of ficus religosa. The natural products researchers throughout the world explored
potential chemical components of ficus religosa. Moreover, the tree grow throughout india
and widely cultivated in south-east asia especially in vicinity of temples (35,36).The ficus
carica is a good source of flavonoids and polyphenols (97,98) with some bioactive compounds
such as arabinose, β-amyrins, β-carotines, glycosides, β-setosterois and xanthotoxol 38,39,93).
There is no systematic evidence been reported for anti-proliferative effect of ficus
racemosa fruits extracts against hepatocellular carcinoma. Therefore, present study was
designed with the study of phytochemical, constituents potential effect of ficus racemosa
fruits extracts against human hepatocellular carcinoma (HepG-2) Cell line.

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MATERIALS AND METHODS
Collection and identification of fruit
Fig were collected from paiyur village, Krishnagiri District, Tamilnadu, India and
identified by Prof. Dr.V.Ravi, Assistant Professor Dept of Botany, Government Arts
College, (Men) Krishnagiri.
Preparation of Fruit Extracts
Fresh fruits were washed then they were shade-dried up to fifteen days and powdered
by maceration method. The dry powders 50 g each were extracted with methanol,
chloroform, hexane, ethyl acetate and Aqueous. Dried powder was soaked separately at
room temperature for 72 h. The extract was filtered using Whatmann filter paper and the
filtrate was concentrated at 45 to 55ºC under reducing pressure using vacuum rotary
evaporator. The yield of extracts was quantified and concentrated crude extracts were
further subjected to biological activity.
Preparation of growth medium
Ten grams of Dulbecco's Modified Eagle's medium (DMEM) was dissolved in 990 ml of
sterilized double distilled water. To this solution, 1.5 g of sodium bicarbonate and 10 ml of
penicillin-streptomycin cocktail were added and mixed thoroughly. Later this medium was
filtered using membrane filter (0.22 μm), dispensed into sterilized container and stored at
4°C. Fetal bovine serum (FBS) (10 %) was added to this medium and used for cell culture.
Crude extract of MTT Assay
Standardization of fruit extracts against human hepatocellular carcinoma (HepG2)
cell line was done by cell viability assay or MTT assay as described by (62). HepG-2 (1 × 104
cells/ml) were plated in 96 well plates with DMEM medium containing 10 % FBS. The
cells were incubated for 24 hours under 5 % CO2 and 95 % O2 at 37°C. The medium was
removed, washed with PBS and fresh serum free medium was added and kept in incubator
for 1 hour. After starvation, the cells were treated with the crude extracts at different
concentrations such as 25, 50, 75, 100 and 125 µg/ml and incubated for 24 and 48 hours.
After incubation 10 μl of 5 mg/ml MTT solution was added to each well and incubated for 4
hour. After incubation, supernatant was aspirated and 100 μl of DMSO was added to
crystals for solubilizing. A micro plate reader was used to measure the absorbance at 570
nm for each well. Percentage of cell viability was calculated as follows.
DAPI Method
HepG-2 cells were plated at 1×106 cells/well into a six well chamber plate. At >90%
confluence, the cells were treated separately with the IC50 concentrations of fruit extracts
along with control for 24 h and 48 h. The cells were washed with PBS, fixed in

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methanol:acetic acid (3:1, v/v) for 10 min. and stained with 5 μl of DAPI for 20 min.
Nuclear morphology of cells was examined under Carl Zeiss Axio vision fluorescent
microscope (Software: Axiovision 4.8).
Phytochemical screening
Phytochemical screening of the sample was carried out as described by (67,78). The
bract extract samples were screened for carbohydrates, alkaloids, flavonoids, phytosterols
and steroids, anthocyanin and beta cyanin, phenols, tannins, saponins, glycosides and
proteins.
FT-IR Analysis
Dried powder form of methanol extract of fruit extract were used for FT-IR analysis.
Precisely, 10 mg of the dried extract powder was encapusulated in 100 mg of KBr pellet, in
order to prepare translucent sample discs. The powdered samples were loaded in FT-IR
spectroscope (Shimadzu, IR Affinity 1, Japan), with a scan range from 4000 to 400 cm-1
with a resolution of 4 cm-1. The output of the results in the form of graphs were analyzed
and the functional groups were identified by the peaks and the reference tables.
GC-MS Analysis
GC-MS spectral analysis was carried out to determine the presence of aromatic
compounds in the extracts. The model of the GC-MS used for mass spectral identification
was an Agilent 7890 interfaced to a 240 mass selective detector with ion trap. The capillary
column (30 m x 0.25 mm x 0.25 μm film thickness) was HP-5MS. The oven temperature
was initially maintained at 80°C to 300°C. The carrier gas used was nitrogen (99.999%), at
a flow rate of 1.0 mL/ min., and injection volume of 1.0 μL was employed (split ratio of
10:1). The electron-impact ionization of the mass spectrometry was operated at electron
energy of 70 eV. Mass spectra were taken at 70 eV, a scan interval of 0.5 seconds and
fragments from 40 to 450 Da. Total GC running time was 61 min.
RESULTS
In the present study, aqueous, chloroform, ethyl acetate, methanol and hexane
extracts of fig fruits, resembled a dark brown coloured with paste consistency and the
powder of dried fruits are soluble in aqueous (double distilled water), chloroform, ethyl
acetate, methanol and hexane.
Cell viability Assay (MTT Assay)
The MTT Assay results showed a profound loss of cell viability in methanol extract
at 50μg/μl ranging about 61% when compared to Chloroform, Ethyl acetate, Aqueous and
Hexane extracts for a period of 48 hours as shown in the table-1. When the fruit extracts
were treated in a dose dependent manner of 25, 50, 75, 100, and 125μg/μl. The cell viability
loss showed a profound decrease about 22% in methanol at 100μg/μl followed by
Chloroform, Ethyl acetate, Aqueous and Hexane where as at 125μg/μl the viability loss

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showed a higher loss an methanol with 22% followed by Chloroform, Ethyl acetate,
Aqueous and Hexane. Hence the MTT Assay results showed that the cell viability loss was
profound in methanol extract when compared with Chloroform, Ethyl acetate, Aqueous and
Hexane with their respective IC 50 values therefore the methanol extract had a profound
anticancer effect on HepG-2 cell line when tested by MTT Assay Fig-1
Table-1;
MTT assay(Fig fruit extracts against HepG-2 cell line)for 24 hrs
Values are mean ± SE of five individual observations.
Values in parenthesis are per cent change over standard
+ denotes per cent increase over standard.
*denotes values are significant at p˂0.01.
Concentration
Methanol
Chloroform
Ethyl acetate
Aqueous
Hexane
25 μg/μl
80.676±0.525*
-19.324
85.522±0.475*
-14.478
91.438±1.002*
-8.562
92.65±0.431*
-7.35
96.586±0.866*
-3.414
50 μg/μl
61.148±0.511*
-38.852
70.416±0.178*
29.584
82.17±0.059*
-17.83
82.17±0.059*
-17.83
90.566±0.370*
-9.434
75 μg/μl
40.69±0.600*
-59.31
54.386±0.391*
-45.614
67.874±0.653*
-32.126
70.378±0.274*
-29.622
83.658±0.263*
-16.342
100 μg/μl
22.784±0.967*
-77.216
47.678±0.608*
-52.322
50.656±0.217*
-49.344
53.144±0.077*
-46.856
76.282±0.295*
-23.718
125 μg/μl
22.784±1.942*
-94.532
36.006±0.549*
-63.994
35.24±0.340*
-64.76
18.754±0.954*
-81.246
60.776±0.117*
-39.224
IC50 values
μg/μl
63.62
93.42
102.2
96.63
143.0

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Fig-1;MTT assay for 24 hrs
Preliminary Phytochemical Screening
In the present study, aqueous, chloroform, ethyl acetate, methanol and hexane
extracts of fig fruits, resembled a dark brown coloured paste and the powder of dried fruits
is highly soluble in aqueous (double distilled water), chloroform, ethyl acetate, methanol
and hexane. The preliminiary phytochemical screening of all the five fruit extracts of fig
showed the presence of Acids, Anthocyanin, Carbohydrates, Cardiac glycosides,
Flavonoids, Phenols, Saponins and Steroids in aqueous extract, Alkaloids, Anthocyanins,
Cardiac glycosides, Flavonoids, Phenols, Saponins and Streoids in Chloroform extract, Ethy
lacetate extract showed the presence of Acids, Alkaloids, Anthocyanins, Carbohydrates,
Cardiac glycosides, Flavonoids, Phenols, Saponins, Steroids, Tanins, Terpenoids and
Triterpenoids, methanol extract showed the presence of Acids, Alkaloids, Anthocyanins,
Carbohydrates, Cardiac glycosides, Coumarins, Flavonoids, Glycosides, Phenols, Saponins,
Steroids, Tanins, Terpenoids and Triterpenoids and whereas Hexane extract showed the
presence of Acids, Anthocyanins, Carbohydrates, Flavonoids, Phenols, Quinones, Saponins,
Steroids and Triterpenoids as shown in the Table : 2

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Table-2 ; Phytochemical screening of various fruit extracts of fig
Secondary
metabolites
Aqueous
Chloroform
Ethyl
acetate
Methanol
Hexane
Acids
+
-
++
++
+
Alkaloids
-
+++
+
+++
-
Anthocyanin
++
+
+++
++
++
Carbohydrate
+++
+++
+++
+++
+++
Cardiac glycosides
++
+
+
++
-
Coumarins
-
+
-
+
-
Flavonoids
++
+++
+
+++
++
Glycosides
++
-
--
+
-
Phenols
+++
+
++
++
++
Protein
-
-
-
-
-
Quinones
+
-
-
-
+
Saponins
+++
+++
+++
+++
+
Steroids
++
+
+++
+++
++
Tannins
++
-
+++
++
-
Terpenoids
++
-
+++
++
-
Triterpenoids
++
+
++
+++
+
+++ : Higly present; ++ : Moderately present; + : Trace; - : Absence
FT-IR Spectral Analysis
FT-IR spectral analysis of Aqueous, Chloroform, Ethyl acetate, Hexane and
Methanol extracts showed prominent aromatic compounds with varying functional groups
such as Alkanes, Alkyls, Alkyl halides, Amides, Alcohol, Aldehydes and ethers etc as
shown in fig. 2 to 6 and table 3 to 7.

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Fig. 2; FT-IR spectra of various functional groups (3500 to 1000 cm-1) obtained from
fig aqueous extract.
Table 3; FT-IR spectral peak values and functional groups (3500 to 1000 cm-1)
obtained from fig aqueous extract.
Fig. 3; FT-IR spectra of various functional groups (3500 to 1000 cm-1) obtained from
fig chloroform extract
Characteristic
absorption cm-1
Functional group
Notes
3327.92 cm-1
Alcohol
O-H stretch
1638.02 cm-1
Aldehydes
C=O stretch
1015.85 cm-1
Alkyl Halides
C-F stretch

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Table 4; FT-IR spectral peak values and functional groups (3500 to 1000 cm-1)
obtained from fig chloroform extract
Characteristic absorption
cm-1
Functional group
Notes
2956.73 cm-1
Alkene and Alkyls
C-H stretch
2927.32 cm-1
Alkene and Alkyls
C-H stretch
2856.18 cm-1
Alkene and Alkyls
C-H stretch
1736.48 cm-1
Alkene and Alkyls
C-H stretch
1464.24 cm-1
Alkene and Alkyls
C-H stretch
1377.34 cm-1
Alkene and Alkyls
C-H stretch
1214.59 cm-1
Ether
=C-O-C sym
1167.11 cm-1
Ether
=C-O-C sym
746.12 cm-1
Alkyl halides
-C-Cl stretch
668.07 cm-1
Alkyl halides
C-Br stretch
Fig. 4: FT-IR spectra of various functional groups (3500 to 1000 cm-1) obtained from
fig ethyl acetate extract

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Table 5: FT-IR spectral peak values and functional groups (3500 to 1000 cm-1)
obtained from fig ethyl acetate extract
Characteristic
absorption cm-1
Functional group
Notes
3293.14 cm-1
Alkynes
=C-H stetch
2933.35 cm-1
Alkynes
=C-H stetch
1736.84 cm-1
Alkene and Alkyl
C-H stretch
1643.07 cm-1
Alkene and Alkyl
C-H stretch
1372.79 cm-1
Alkyl halides
C-F stretch
1236.41 cm-1
Alkyl halides
C-F stretch
755.83 cm-1
Alkynes
=CH bend
607.54 cm-1
Alkynes
=CH bend
Fig. 5: FT-IR spectra of various functional groups (3500 to 1000 cm-1) obtained from
fig hexane extract

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Table 6: FT-IR spectral peak values and functional groups (3500 to 1000 cm-1)
obtained from fig hexane extract
Fig. 6: FT-IR spectra of various functional groups (3500 to 1000 cm-1) obtained from
Characteristic absorption
cm-1
Functional group
Notes
2956.23 cm-1
Alkanes and Alkyls
C-H stretch
2924.71 cm-1
Alkanes and Alkyls
C-H stretch
2872.71 cm-1
Alkanes and Alkyls
C-H stretch
1748.08 cm-1
Alkanes and Alkyls
C-H stretch
1460.25 cm-1
Alkanes and Alkyls
C-H stretch
1376.88 cm-1
Alkanes and Alkyls
C-H stretch
1237.03 cm-1
Alkyl halides
C-F stretch
1161.87 cm-1
Alkyl halides
C-F stretch
1048.59 cm-1
Alkyl halides
C-F stretch
983.28 cm-1
Alkenes
=C-H bend
724.58 cm-1
Alkenes
C-H bend

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fig methanol extract
GC-MS spectral analysis
The results pertaining to GC-MSanalysis led to the identification of number of
compounds from the GC fractions of Aqueous, Chloroform, Ethyl acetate, Hexane and
Methanol extracts of fig fruit. The active principles with their retention time (RT) as shown
in the mass spectras with the respective library match, RT, peak name and peak area with
NIST/NBS spectral database nearly more than 80 compounds were identified with their
peak value and RT as presented in table-8 to 12. The mass spectra and their mass peaks are
given in Fig-7 to 11 respectively.
Table 7: FT-IR spectral peak values and functional groups (3500 to 1000 cm-1)
obtained from fig methanol extract
Characteristic absorption
cm-1
Functional group
Notes
3297.71 cm-1
Alkynes
C-H stretch
2939.26 cm-1
Alkanes
C-H stretch
2837.75 cm-1
Amide
N-H bend
1643.44 cm-1
Alkyl halides
C-F stretch
1375.39 cm-1
Alkyl halides
C-F stretch
1256.13 cm-1
Alkyl halides
C-F stretch
1013.41 cm-1
Alkyl halides
C-F stretch
772.27 cm-1
C-H bend
630.17 cm-1
Alkynes
= CH bend

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Fig-7; GC-MS chromatogram of fig aqueous extract
Table-8; GC-MS spectral data of fig fruit aqueous extract
S.No
RT
Peak name
Area
R match
1
27.611
2-Pentadecanone, 6,10,14-tri
1.851e+6
729
2
31.158
n-Hexadecanoic acid
2.297e+6
805
3
31.379
Ethyl 13-methyl-tetradecanoa
1.090e+7
735
4
31.605
Bicyclo[4.3.0]nonan-2-one, 8
997467
763
5
33.662
Ethyl 15-methyl-hexadecanoat
635960
796
6
33.938
Phthalic acid, butyl 2-penty
335868
909
7
34.122
3,7,11,15-Tetramethyl-2-hexa
1.544e+7
831
8
34.379
Methyl 14-methyl-eicosanoate
1.079e+6
589
9
34.597
Methyl 9,10-methylene-hexade
993556
656
10
35.676
n-Propyl 9-octadecenoate
3.008e+6
815

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11
35.897
n-Propyl 9,12-octadecadienoa
1.365e+7
847
12
36.374
Ethyl 9,12,15-octadecatrieno
6.122e+6
871
13
36.523
Octadecanoic acid, 10-methyl
126896
648
14
37.037
Androstan-17-one, 3-ethyl-3-
349788
669
15
38.308
3,7,11,15-Tetramethyl-2-hexa
385862
812
16
39.975
Methyl 19-methyl-eicosanoate
1.390e+6
779
17
40.494
Octadecane, 1-(ethenyloxy)-
665999
756
18
40.636
2H-Pyran-2-one, tetrahydro-6
614429
752
19
41.918
Methyl 20-methyl-heneicosano
432745
724
20
42.405
Tetratriacontyl heptafluorob
1.040e+6
771
21
43.798
Methyl 20-methyl-docosanoate
1.432e+6
730
22
44.247
Dotriacontyl pentafluoroprop
2.157e+6
785
23
45.608
Tetracosanoic acid, methyl e
1.001e+6
691
24
46.025
Octatriacontyl pentafluoropr
2.422e+6
807
25
46.506
2-Nonadecanone 2,4-dinitroph
151240
566
26
47.357
Methyl 17-methyl-tetracosano
2.091e+6
720
27
47.743
Hexatriacontyl pentafluoropr
2.865e+6
813
28
48.197
2,6,10,14,18,22-Tetracosahex
901596
826
29
49.047
Hexacosanoic acid, methyl es
445119
670
30
49.403
Tetratriacontyl heptafluorob
2.833e+6
750
31
49.760
1,13-Bis(4-acetylphenyl)trid
988124
557
32
50.684
Methyl 20-methyl-hexacosanoa
899937
576
33
51.010
Tetratriacontyl pentafluorop
1.661e+6
773
34
51.117
34 Kauran-18-oic acid, 7-(acety
314884
709
35
52.280
Dehydroergosterol 3,5-dinitr
1.719e+6
693
36
52.582
Cyclooctacosane
632457
756
37
52.720
Anthiaergosatn-5,7,9,22-tetr
291913
664

© 2019 IJRAR March 2019, Volume 6, Issue 1 www.ijrar.org (E-ISSN 2348-1269, P- ISSN 2349-5138)
IJRAR19H1121
International Journal of Research and Analytical Reviews (IJRAR) www.ijrar.org
799
38
52.953
5Alpha-cyano-3-methoxymethyl
287666
546
39
53.419
5H-Cyclopropa(3,4)benz(1,2-e
551294
454
40
53.568
9,19-Cyclochloestene-3,7-dio
857206
599
41
53.718
gamma.-Tocopherol
2.492e+6
627
42
53.822
Triacontanoic acid
906981
527
43
53.897
Tungsten, pentacarbonyl(4,5-
188250
475
44
54.097
Triacontyl trifluoroacetate
993352
728
45
54.427
Pregn-4-ene-3,11,20-trione,
245078
445
46
54.522
Pregn-5-en-20-one, 3-(acetyl
730592
594
47
54.722
alpha.-Tocopherolquinone
938246
633
48
54.949
Silanamine, N-[(17.beta.)-3,
5.414e+6
816
49
55.138
17-(1,5-Dimethylhexyl)-10,13
1.246e+7
861
50
55.538
Boron, bis[.mu.-[3,5-bis(1,1
2.750e+6
523
51
55.629
Rubixanthin acetate
696347
399
52
55.750
Lycopene
1.034e+6
426
53
55.900
Stigmasterol
2.592e+6
632
54
55.958
Reserpine
1.713e+6
555
55
56.139
3-[18-(3-Hydroxy-propyl)-3,3
2.381e+6
425
56
56.275
Bis(.eta.-5-cyclopentadienyl
1.481e+6
440
57
56.309
Pregn-4-ene-3,11,20-trione,
546086
409
58
56.413
Ergosterol
3.387e+6
575
59
56.573
Dehydroergosterol 3,5-dinitr
9.387e+6
748
60
56.917
Campesterol
1.207e+8
848
61
57.360
Stigmasterol
1.820e+8
827
62
57.635
Stigmasterol
7.628e+6
667
63
57.798
D-Homo-24-nor-17-oxachola-20
3.077e+6
471
64
57.863
Cholest-5-en-3-one
2.448e+6
625

© 2019 IJRAR March 2019, Volume 6, Issue 1 www.ijrar.org (E-ISSN 2348-1269, P- ISSN 2349-5138)
IJRAR19H1121
International Journal of Research and Analytical Reviews (IJRAR) www.ijrar.org
800
Fig-
8;
GC-MS chromatogram of fig fruit methanolic extract
Table -9; GC-MS spectral data of fig fruit Methanolic extract
S.No.
RT
Peak Name
Area
R. Match
1
31.161
n-Hexadecanoic acid
3.044e+6
813
2
34.116
3,7,11,15-Tetramethyl-2-hexa
3.636e+6
820
65
57.882
Cholest-5-en-3-one
4.260e+6
596
66
58.039
2-(16-Acetoxy-11-hydroxy-4,8
5.237e+6
466
67
58.200
9,19-Cyclolanost-7-en-3-ol
9.174e+6
566
68
58.449
gamma.-Sitosterol
1.685e+8
854
69
58.727
gamma.-Sitosterol
1.671e+7
732
70
58.885
gamma.-Sitosterol
1.658e+6
632
71
59.004
Molybdenum, dicarbonyl-bis(.
9.688e+6
563
72
59.195
Stigmasta-5,24(28)-dien-3-ol
1.990e+7
822
73
59.322
Stigmasta-5,24(28)-dien-3-ol
993652
751
74
59.449
9,19-Cycloergost-24(28)-en-3
4.074e+7
765
75
59.723
4,4,6a,6b,8a,11,11,14b-Octam
1.163e+7
759
76
59.934
Oleana-11,13(18)-diene
6.751e+6
660
77
60.102
Cholest-5-en-3-one
1.077e+7
670
78
60.255
17-(1,5-Dimethyl-3-phenylthi
3.206e+7
744
79
60.460
Urs-9(11)-en-12-one-28-oic a
216221
460
80
60.627
4,22-Stigmastadiene-3-one
2.849e+7
768

© 2019 IJRAR March 2019, Volume 6, Issue 1 www.ijrar.org (E-ISSN 2348-1269, P- ISSN 2349-5138)
IJRAR19H1121
International Journal of Research and Analytical Reviews (IJRAR) www.ijrar.org
801
3
34.594
Methyl 14-methyl-eicosanoate
1.556e+6
651
4
35.628
6-Octadecenoic acid
1.167e+6
834
5
35.736
Oleic Acid
1.568e+6
725
6
35.857
Ethyl 9,12-hexadecadienoate
3.422e+6
825
7
36.354
Methyl 8,11,14-heptadecatrie
504121
771
8
36.440
9,12,15-Octadecatrienoic aci
362722
590
9
39.442
Hexadecanoic acid, 1-(hydrox
143303
590
10
40.491
Acetic acid, 17-(4-hydroxy-5
177383
479
11
40.634
Cholestan-3-one, 4,4-dimethy
501521
672
12
40.910
9,19-Cyclolanostan-3-ol,
579579
758
13
43.600
Stigmast-4-en-3-one
1.289e+6
823
14
44.244
6-[4-(Tetrahydro-pyran-2-ylo
234739
609
15
46.023
Tetracontane, 3,5,24-trimeth
274327
806
16
47.737
Triacontyl trifluoroacetate
214715
674
17
51.004
Dodecanoic acid, 1a,2,5,5a,6
2.761e+6
628
18
52.284
Anthiaergosatn-5,7,9,22-tetr
408250
607
19
52.996
12-Cyclohex-3-enyl-3-methyl-
498736
703
20
53.539
Milbemycin B, 6,28-anhydro-1
392438
510
21
53.576
Rhodopin
236717
500
22
53.713
.gamma.-Tocopherol
2.313e+6
623
23
53.838
2,4,6,8-Tetradecatetraenoic
1.019e+6
551
24
53.880
2,4,6-Decatrienoic acid, 1a,
579397
577
25
54.562
Pregn-4-ene-3,11,20-trione,
3.369e+6
644
26
54.778
1,4:5,8-Dimethanonaphthalene
343002
438
27
54.948
Silanamine, N-[(17.beta.)-3,
2.184e+6
814
28
55.134
17-(1,5-Dimethylhexyl)-10,13
1.585e+6
653
29
55.424
3-[18-(3-Hydroxy-propyl)-3,3
58455
437

© 2019 IJRAR March 2019, Volume 6, Issue 1 www.ijrar.org (E-ISSN 2348-1269, P- ISSN 2349-5138)
IJRAR19H1121
International Journal of Research and Analytical Reviews (IJRAR) www.ijrar.org
802
30
55.477
Milbemycin B, 5-demethoxy-5-
86943
437
31
55.539
3-[18-(3-Hydroxy-propyl)-3,3
211089
473
32
55.595
Pregn-4-ene-3,11,20-trione,
279608
413
33
55.657
4'-Apo-.beta.,.psi.-caroteno
391642
460
34
55.704
[5-(5-Cyano-3,4-dimethyl-1H-
188057
416
35
55.780
Prosta-5,13-dien-1-oic acid,
571434
484
36
55.820
Lycopene
568401
482
37
55.869
1',1'-Dicarboethoxy-1.beta.,
468643
509
38
55.896
Astaxanthin
40290
489
39
55.926
Milbemycin B, 5-demethoxy-5-
354477
456
40
55.954
5H-Cyclopropa(3,4)benz(1,2-e
364897
429
41
55.986
Milbemycin B, 5-demethoxy-5-
276782
428
42
56.048
.psi.,.psi.-Carotene, 1,1',2
929204
409
43
56.146
Milbemycin B, 5-demethoxy-5-
1.068e+6
412
44
56.424
Cholest-4-ene-3,6-dione
9.676e+6
668
45
56.549
D-Glucopyranoside, (3.beta.,
4.054e+6
553
46
56.641
3-[3-(5,5-Dimethyl-[1,3]diox
776355
542
47
56.671
Prosta-5,13-dien-1-oic acid,
1.275e+6
483
48
56.902
Campesterol
3.229e+7
788
49
57.160
Lycoxanthin
2.578e+6
490
50
57.319
Lycoxanthin
4.450e+7
830
51
57.530
Stigmasterol
1.551e+6
607
52
57.575
5H-Cyclopropa(3,4)benz(1,2-e
2.127e+6
483
53
57.634
Astaxanthin
2.391e+6
452
54
57.768
2,2-Bis[4-[[4-chloro-6-(3-et
1.676e+6
446
55
57.860
Cholest-5-en-3-one
3.344e+6
564
56
57.915
Acetic acid, 1,1',4'-triacet
1.014e+6
432

© 2019 IJRAR March 2019, Volume 6, Issue 1 www.ijrar.org (E-ISSN 2348-1269, P- ISSN 2349-5138)
IJRAR19H1121
International Journal of Research and Analytical Reviews (IJRAR) www.ijrar.org
803
57
57.947
Echinenone
932194
455
58
57.984
1',1'-Dicarboethoxy-1.beta.,
1.364e+6
495
59
58.042
Rhodoxanthin
1.244e+6
440
60
58.080
8-[2-(2-Amino-phenyl)-2-oxo-
812250
475
61
58.123
8-[2-(2-Amino-phenyl)-2-oxo-
1.230e+6
470
62
58.167
9,19-Cyclochloestene-3,7-dio
4.498e+6
553
63
58.404
gamma.-Sitosterol
5.398e+7
811
64
58.673
Lycoxanthin
3.176e+6
542
65
58.715
Ergost-5-ene-3,25-diol,
1.852e+6
690
66
58.770
Cholest-8(14)-en-3-ol, 2,2-d
2.935e+6
642
67
58.866
8,12-Di-O-acetylingol 7-(4'-
881230
439
68
58.908
Milbemycin B, 5-demethoxy-5-
805627
444
69
58.969
3-[18-(3-Hydroxy-propyl)-3,3
1.509e+6
456
70
59.000
Urs-9(11)-en-12-one-28-oic a
551032
430
71
59.052
Urs-9(11)-en-12-one-28-oic a
1.465e+6
430
72
59.191
Stigmasta-5,24(28)-dien-3-ol
6.010e+6
727
73
59.424
9,19-Cycloergost-24(28)-en-3
1.041e+7
743
74
59.557
Tungsten, pentacarbonyl(4,5-
956923
443
75
59.608
Pregn-4-ene-3,11,20-trione,
522967
399
76
59.721
D-Homo-24-nor-17-oxachola-20
2.582e+6
487
77
59.788
Milbemycin B, 5-demethoxy-5-
459840
424
78
59.932
Acetic acid, 17-(4-hydroxy-5
4.511e+6
547
79
60.011
Rhodopin
517576
518
80
60.051
Cholest-5-en-3-one
1.206e+6
628
81
60.092
Cholest-5-en-3-one
963474
620
82
60.249
9,19-Cyclo-25,26-epoxyergost
6.648e+6
641
83
60.382
1',1'-Dicarboethoxy-1.beta.,
269278
459

© 2019 IJRAR March 2019, Volume 6, Issue 1 www.ijrar.org (E-ISSN 2348-1269, P- ISSN 2349-5138)
IJRAR19H1121
International Journal of Research and Analytical Reviews (IJRAR) www.ijrar.org
804
84
60.485
3-[18-(3-Hydroxy-propyl)-3,3
417864
446
85
60.611
Lanosterol
6.640e+6
710
86
60.768
4,7-Benzofurandione, 3-acety
94910
470
87
60.798
1',1'-Dicarboethoxy-1.beta.,
87206
460
88
60.828
.psi.,.psi.-Carotene, 1,1',2
37802
424
89
60.858
1,4:5,8-Dimethanonaphthalene
27767
459
Fig-9; GC-MS Chromatogram Of Hexane Extract

© 2019 IJRAR March 2019, Volume 6, Issue 1 www.ijrar.org (E-ISSN 2348-1269, P- ISSN 2349-5138)
IJRAR19H1121
International Journal of Research and Analytical Reviews (IJRAR) www.ijrar.org
805
Table -10; GC-MS spectral data of fig fruit Hexane extract
S.No
RT
Peak name
Area
R match
1
31.222
n-Hexadecanoic acid
6.217e+6
811
2
31.378
Ethyl 13-methyl-tetradecanoa
.727e+6
731
3
34.121
Phytol
2.918e+7
794
4
35.659
6-Octadecenoic acid
4.036e+6
815
5
35.772
Octadecanoic acid, 2-hydroxy
2.660e+6
624
6
35.898
Methyl 7,11,14-eicosatrienoa
1.195e+7
803
7
36.093
Lup-20(29)-en-3-one
247217
758
8
36.118
Lup-20(29)-en-3-one
250795
726
9
36.182
Lup-20(29)-en-3-one
156565
774
10
36.239
Lup-20(29)-en-3-one
724861
737
11
36.371
Methyl 8,11,14-heptadecatrie
1.136e+7
828
12
36.473
Lup-20(29)-en-3-one
1.057e+6
726
13
36.593
Lup-20(29)-en-3-one
5.159e+6
789
14
36.648
Lup-20(29)-en-3-one
2.694e+6
755
15
36.722
Lup-20(29)-en-3-one
507107
689
16
36.836
Lup-20(29)-en-3-one
2.118e+6
755
17
36.897
Lup-20(29)-en-3-one
1.212e+6
753
18
36.936
Lup-20(29)-en-3-one
1.082e+6
724
19
36.973
Lup-20(29)-en-3-one
1.148e+6
714
20
37.626
Lup-20(29)-en-3-one
2.789e+7
831
21
37.697
Lup-20(29)-en-3-one
4.155e+7
814
22
38.479
Benzoic acid, 2,3-dimethyl-6
456203
586
23
39.221
1-Phenanthrenol, 1,2,3,4,4a,
8.478e+6
676
24
40.905
Tricyclo[20.8.0.0(7,16)]tria
1.138e+6
614
25
41.966
Lupeol
5.633e+7
781

© 2019 IJRAR March 2019, Volume 6, Issue 1 www.ijrar.org (E-ISSN 2348-1269, P- ISSN 2349-5138)
IJRAR19H1121
International Journal of Research and Analytical Reviews (IJRAR) www.ijrar.org
806
26
42.025
Lupeol
1.925e+7
765
27
42.084
Lupeol
2.324e+7
769
28
42.217
6a,14a-Methanopicene, perhyd
8.605e+6
651
29
42.276
Lupeol
2.007e+7
655
30
42.850
Trilinolein
614056
565
31
56.912
Campesterol
5.956e+7
835
32
57.336
Stigmasterol
1.110e+8
838
33
57.864
Milbemycin B, 5-demethoxy-5-
808294
487
34
58.092
Prosta-5,13-dien-1-oic acid
521059
453
35
58.188
Stigmasterol
4.640e+6
582
36
58.444
gamma.-Sitosterol
1.781e+8
844
37
58.715
gamma.-Sitosterol
1.803e+7
745
38
58.997
3-(1,5-Dimethyl-hexyl)-3a,10
8.657e+6
713
39
59.182
Cholest-5-en-3-ol, 24-propyl
6.603e+6
768
40
59.393
3,7,11,15-Tetramethyl-2-hexa
2.602e+7
811
41
59.719
4,4,6a,6b,8a,11,11,14b-Octam
7.053e+6
764
42
59.931
Gorgostan-3-ol, 5,6-dichloro
2.337e+6
590
43
60.100
Betulin
4.633e+6
621
44
60.199
beta.-Amyrin
5.902e+6
768
45
60.272
Lup-20(29)-en-3-one
7.458e+6
711
46
60.615
9,19-Cyclolanost-24-en-3-ol,
1.712e+7
854

© 2019 IJRAR March 2019, Volume 6, Issue 1 www.ijrar.org (E-ISSN 2348-1269, P- ISSN 2349-5138)
IJRAR19H1121
International Journal of Research and Analytical Reviews (IJRAR) www.ijrar.org
807
Fig-10; GC-MS chromatogram of fig fruit chloroform extract
Table-11; GC-MS spectral data of fig fruit chloroform extract
S.No
RT
Peak Name
Peak Area
R Match
1
22.072
2H-1-Benzopyran-2-one
1.426e+7
918
2
24.314
Flamenol
1.739e+7
889
3
26.201
3,7,11,15-Tetramethyl-2-hexa
3.615e+6
788
4
27.419
3,7,11,15-Tetramethyl-2-hexa
834626
787
5
27.613
2-Pentadecanone, 6,10,14-tri
477839
730
6
31.239
n-Hexadecanoic acid
3.346e+7
802
7
31.380
Ethyl 13-methyl-tetradecanoa
1.217e+7
741
8
31.583
l-(+)-Ascorbic acid 2,6-dihe
1.903e+6
697
9
33.657
Ethyl 14-methyl-hexadecanoat
1.550e+6
735
10
33.936
Phthalic acid, butyl hexyl e
435764
907
11
34.113
Phytol
1.233e+7
801
12
35.668
6-Octadecenoic acid
1.229e+7
836
13
35.798
Octadecanoic acid
8.120e+6
746

© 2019 IJRAR March 2019, Volume 6, Issue 1 www.ijrar.org (E-ISSN 2348-1269, P- ISSN 2349-5138)
IJRAR19H1121
International Journal of Research and Analytical Reviews (IJRAR) www.ijrar.org
808
14
35.915
Methyl 9-cis,11-trans-octade
3.689e+7
848
15
36.102
Ethyl 9,12-hexadecadienoate
1.297e+6
822
16
36.387
Ethyl 9,12,15-octadecatrieno
2.819e+7
858
17
39.978
Methyl 19-methyl-eicosanoate
444249
757
18
40.632
2H-Pyran-2-one, tetrahydro-6
702593
742
19
45.926
2-Propen-1-one, 1-(2,6-dihyd
4.274e+6
845
20
47.351
Pentacosanoic acid, methyl e
1.125e+6
693
21
47.742
Tetratriacontyl heptafluorob
573333
780
22
48.208
Squalene
4.130e+7
840
23
48.694
4H-1-Benzopyran-4-one, 7-(.b
1.087e+6
556
24
49.240
4,6-Diamino-3-[p-methoxyphen
500191
746
25
49.428
N,N'-Bis(p-methoxybenzyliden
4.085e+6
553
26
49.545
4H-1-Benzopyran-4-one, 5-hyd
2.695e+6
749
27
49.746
N,N'-Bis(p-methoxybenzyliden
5.168e+6
549
28
50.255
D-Homo-24-nor-17-oxachola-1,
804000
552
29
50.724
1,6,10,14,18,22-Tetracosahex
5.411e+6
749
30
50.849
Vinbarbital
1.256e+6
672
31
50.911
9,19-Cyclolanost-24-en-3-ol,
1.879e+6
739
32
51.008
Octatriacontyl trifluoroacet
1.327e+6
730
33
51.274
4H-1-Benzopyran-4-one, 3-hyd
3.492e+7
800
34
51.888
psi.,.psi.-Carotene, 7,7',8
735838
657
35
52.043
5,5'-Bis(1,1-dimethylethyl)-
1.072e+7
860
36
52.334
1,6,10,14,18,22-Tetracosahex
2.685e+6
766
37
52.590
1-Methyl-5-(5-nitro-2-furyl)
2.399e+6
594
38
53.113
Trilinolein
432351
577
39
53.367
Silane, dimethyl(dimethylpen
2.249e+6
630
40
53.566
Milbemycin B, 5-demethoxy-5-
873286
524

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41
53.717
gamma.-Tocopherol
1.268e+7
615
42
53.889
1-Methyl-5-(5-nitro-2-furyl)
2.174e+6
567
43
54.013
Cholestano[7,8-a]cyclobutane
1.112e+6
506
44
54.084
17-Pentatriacontene
1.763e+6
620
45
54.543
2,4,6-Decatrienoic acid, 1a,
1.048e+6
482
46
54.631
Lanostane-7,11-dione, 3,18-b
113887
516
47
54.949
dl-.alpha.-Tocopherol
5.956e+7
572
48
55.127
17-(1,5-Dimethylhexyl)-10,13
6.069e+6
781
49
55.269
7,8,12-Tri-O-acetyl-3-desoxy
551100
434
50
55.340
Silanamine, N-[(17.beta.)-3,
1.398e+6
777
51
55.614
Pregn-4-ene-3,11,20-trione,
780574
490
52
55.896
Milbemycin B, 5-demethoxy-5-
640155
484
53
55.925
Milbemycin B, 5-demethoxy-5-
426196
525
54
56.038
Silane, dimethyl(4-(2-phenyl
4.441e+7
848
55
56.294
17.beta.-Acetoxy-1',1'-dicar
240418
494
56
56.460
Milbemycin B, 5-demethoxy-5-
427196
411
57
56.548
Anthiaergosatn-5,7,9,22-tetr
331406
621
58
56.577
Milbemycin B, 5-demethoxy-5-
345526
452
59
56.682
Rubixanthin acetate
2.362e+6
471
60
56.902
Campesterol
3.438e+7
826
61
57.035
Ergosterol
7.771e+6
702
62
57.319
Stigmasterol
5.134e+7
834
63
57.552
Astaxanthin
963264
568
64
57.597
Cholestano[7,8-a]cyclobutane
2.825e+6
543
65
57.708
Pregn-4-ene-3,11,20-trione,
1.067e+6
423
66
57.851
Decanoic acid, 1,1a,1b,4,4a,
1.782e+6
426
67
57.901
[8,8'-Bi-2H-naphtho[1,8-bc]f
1.397e+6
413

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68
58.089
(25S)-3Beta-acetoxy-5alpha,2
2.900e+6
489
69
58.180
Glycine, N-[(3.alpha.,5.beta
3.741e+6
527
70
58.417
gamma.-Sitosterol
1.026e+8
841
71
58.604
beta.-Sitosterol
1.504e+6
718
72
58.682
beta.-Sitosterol
1.249e+7
721
73
58.933
Cholestano[7,8-a]cyclobutane
1.666e+6
481
74
58.991
Rhodopin
3.836e+6
465
75
59.166
Cholest-5-en-3-ol, 24-propyl
9.637e+6
774
76
59.378
3,7,11,15-Tetramethyl-2-hexa
3.916e+6
805
77
59.403
Trilinolein
3.059e+6
595
78
59.559
4,5,6,7-Tetrahydroxy-1,8,8,9
4.623e+6
754
79
59.702
Phenol, 2-methoxy-6-(3,7,11,
2.429e+6
490
80
59.915
Acetic acid, 17-(4-hydroxy-5
3.303e+6
509
81
60.088
Cholest-5-en-3-one
2.029e+6
606
82
60.133
alpha.-Amyrin
1.034e+6
671
83
60.276
7-Ethyl-cis-4a,trans-4b,cis-
1.156e+7
608
84
60.404
Acetic acid, 6-[1,3]dithian-
316577
431
85
60.456
Bis(.eta.-5-cyclopentadienyl
256200
434
86
60.609
9,19-Cyclolanost-24-en-3-ol,
2.043e+7
872
87
60.873
Lanosterol
2.407e+6
561

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Fig-11; GC-MS chromatogram of fig fruit ethyl acetate extract

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Table-12; GC-MS spectral data of fig fruit ethyl acetate extract
S.No
RT
Peak name
Area
R match
1
31.169
n-Hexadecanoic acid
5.280e+6
798
2
32.210
2-Propenoic acid, 3-(4-hydro
1.904e+6
894
3
34.113
3,7,11,15-Tetramethyl-2-hexa
5.897e+6
812
4
34.332
Methyl 13-octadecenoate
1.067e+6
836
5
34.552
Methyl 9-cis,11-trans-octade
3.701e+6
854
6
35.035
Ethyl 9,12,15-octadecatrieno
1.274e+6
833
7
35.628
6-Octadecenoic acid
2.178e+6
829
8
35.744
Oleic Acid
2.584e+6
732
9
35.864
Methyl 11,14-octadecadienoat
6.802e+6
844
10
36.341
9,12,15-Octadecatrienoic aci
2.593e+6
838
11
38.757
Eicosanoic acid, methyl este
417622
785
12
46.342
Eicosanoic acid, methyl este
816783
775
13
50.325
9,19-Cyclo-25,26-epoxyergost
486827
563
14
50.705
psi.,.psi.-Carotene, 7,7',8
687991
614
15
51.397
Bi-1-cyclohexen-1-yl, 3,3,3'
1.676e+6
691
16
51.940
2H-1-Benzopyran-6-ol, 3,4-di
742176
588
17
52.277
Anthiaergosatn-5,7,9,22-tetr
231652
664
18
53.710
Silane, dimethyl(dimethylpen
3.285e+6
643
19
54.574
2,4,6-Decatrienoic acid, 1a,
3.598e+6
612
20
54.636
17.beta.-Acetoxy-1',1'-dicar
995456
583
21
54.828
4,7-Benzofurandione, 3-acety
28617
490

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813
22
54.940
Silanamine, N-[(17.beta.)-3,
4.520e+6
811
23
55.127
17-(1,5-Dimethylhexyl)-10,13
5.562e+6
838
24
55.244
Rhodoxanthin
269865
423
25
55.358
5H-Cyclopropa(3,4)benz(1,2-e
346802
434
26
55.451
Bis(.eta.-5-cyclopentadienyl
237787
420
27
55.555
Cholestano[7,8-a]cyclobutane
905898
465
28
55.647
1',1'-Dicarboethoxy-1.beta.,
198301
486
29
55.725
Rubrene
576514
434
30
55.772
4,7-Benzofurandione, 3-acety
380526
457
31
55.868
Glycocholic acid
1.357e+6
513
32
55.941
Urs-12-ene-3.beta.,11.beta.-
643005
503
33
55.971
Urs-12-ene-3.beta.,11.beta.-
446068
489
34
56.050
Rhodopin
1.281e+6
494
35
56.094
Milbemycin B, 5-demethoxy-5-
207740
440
36
56.121
Milbemycin B, 5-demethoxy-5-
372022
434
37
56.156
Tetradecanoic acid, 9a-(acet
302552
413
38
56.210
1,4:5,8-Dimethanonaphthalene
728025
459
39
56.446
9,19-Cyclochloestene-3,7-dio
6.857e+6
606
40
56.561
Anthiaergosatn-5,7,9,22-tetr
4.274e+6
647
41
56.693
7-Ethyl-cis-4a,trans-4b,cis-
4.289e+6
590
42
56.900
Campesterol
6.461e+7
840
43
57.334
Stigmasterol
1.186e+8
824
44
57.619
Stigmasterol
2.012e+6
619
45
57.660
Stigmasterol
3.104e+6
592
46
57.780
Cholestano[7,8-a]cyclobutane
2.129e+6
521
47
57.807
Milbemycin B, 5-demethoxy-5-
1.052e+6
480
48
57.877
Stigmasterol
5.433e+6
574

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814
DISCUSSION
India is one of the 12 mega biodiversity centers having 45,000 plant species; its
diversity is unmatched due to the 16 different agro-climatic zones, 10 vegetative zones and
15 biotic provinces. The country has a rich floral diversity of flowering plants (15,000 -
18,000), fungi (23,000), algae (25,000), lichens (1,600), bryophytes (1,800) and
microorganisms (30 million) (105). Traditional medicine is the synthesis of therapeutic
experience of generations of practicing physicians of indigenous systems of medicine.
Traditional preparation comprises medicinal plants, minerals and organic matters etc (55).
Herbal drug constitutes only those traditional medicines that primarily use medicinal plant
49
57.992
2,2-Bis[4-[[4-chloro-6-(3-et
834158
448
50
58.016
5H-Cyclopropa(3,4)benz(1,2-e
1.264e+6
439
51
58.184
Silane, trimethyl[(4.alpha.-
9.133e+6
613
52
58.406
gamma.-Sitosterol
5.440e+7
838
53
58.697
Lycopene
8.923e+6
551
54
58.994
Betulin
5.495e+6
572
55
59.174
Cholest-5-en-3-ol, 24-propyl
2.787e+6
726
56
59.203
Cholest-5-en-3-ol, 24-propyl
3.314e+6
740
57
59.416
9,19-Cycloergost-24(28)-en-3
8.678e+6
738
58
59.589
59.589 Tungsten, pentacarbonyl(4,5-
269770
453
59
59.714
4,4,6a,6b,8a,11,11,14b-Octam
2.030e+7
816
60
59.855
Phenol, 2-methoxy-6-(3,7,11,
101015
427
61
59.930
25-Nor-9,19-cyclolanostan-24
2.306e+6
530
62
59.988
Cholest-5-en-3-one
404373
568
63
60.184
beta.-Amyrin
2.131e+7
830
64
60.269
4,4,6a,6b,8a,11,11,14b-Octam
5.848e+6
725
65
60.634
4,22-Stigmastadiene-3-one
1.000e+7
762
66
60.784
1,4:5,8-Dimethanonaphthalene
92660
488
67
60.870
1',1'-Dicarboethoxy-1.beta.,
242291
415
68
60.902
4,7-Benzofurandione, 3-acety
46552
453

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815
preparations for therapy. The ancient record is evidencing their use by Indian, Chinese,
Egyptian, Greek, Roman and Syrian dates back to about 5000 years as stated by (105).
According to (19,20), nearly 500 plants with medicinal use are mentioned in ancient
texts and around 800 plants have been used in indigenous systems of medicine. Indian
subcontinent is a vast repository of medicinal plants that are used in traditional medical
treatments, which also forms a rich source of knowledge. The various indigenous systems
such as Siddha, Ayurveda, Unani and Allopathy use several plant species to treat different
ailments (54,72). In India around 20,000 medicinal plant species have been recorded recently
(22,23), but more than 500 traditional communities use about 800 plant species for curing
different diseases (51,56).
Currently 80% of the world population depends on plant-derived medicine for the
first line of primary health care for human alleviation because it has no side effects (19,89).
Plants are important sources of medicines and presently about 25% of pharmaceutical
prescriptions in the world contain at least one plant-derived ingredient. In the last century,
roughly 121 pharmaceutical products were formulated based on the traditional knowledge
obtained from various source (105).
Traditionally a most important source of human food in its earliest cultivation.
Mediterranean countries ranks about seventy percent of the world fig fruit production. The
figs are important part of Mediterranean diet, related to health and longevity (88).
Antioxidant compounds such as phenolics, organic acids, vitamin E, and carotenoids,
scavenge free radicals, thus inhibiting the oxidative mechanisms to degenerative illnesses
(80,81).
FT-IR spectral analysis of aqueous, chloroform, ethyl acetate, hexane and hexane
extracts showed prominent aromatic compounds with varying functional groups such as
alkanes, alkyls, alkyl halides, amides, alcohol, aldehydes
GC-MS spectral analysis results pertaining to GC-MSanalysis led to the
identification of number of compounds from the GC fractions of aqueous, chloroform, ethyl
acetate, hexane and methanol extracts of fig fruit. The active principles with their retention
time (RT) as shown in the mass spectras with the respective library match, RT, peak name
and peak area with NIST/NBS spectral database. The presence of phenols flavonids in GC-
MS suggests that ficus carica fruit is pharmacologically active, supporting the claim by (61)
Initiation of hepatic oncogenesis, transforms hepatocytes elude various cellular
defensive activities and acquire abnormal capabilities to survive and proliferate (41,42). The
receptor tyrosine kinases plays an important role (14,15) in signaling of hepatocellular
carcinoma, its development and progression.

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CONCLUSION
In conclusion, the methanol extract of ficus racemosa fruit has demonstrated
promising anticancer properties against human hepatocellular carcinoma (HepG-2) cells by
in vitro method. Increasing awareness, promotion and utilization of this fruit for public
benefits are highly encouraged and identification of active phytoconstiuents in the extracts
will serve as a natural cytotoxic agent against various ailments.
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