This paper is devoted to the study of unbounded derivations on Banach quasi *-algebras with a particular emphasis to the case when they are infinitesimal generators of one-parameter automorphisms groups. Both of them, derivations and automorphisms are considered in a weak sense, i.e., with the use of a certain families of bounded sesquilinear forms. Conditions for a weak *-derivation to be the generator of a *-automorphisms group are given. © 2019, Fondazione Annali di Matematica Pura ed Applicata and Springer-Verlag GmbH Germany, part of Springer Nature.