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6th BIOPESTICIDE INTERNATIONAL CONFERENCE (BIOCICON –
2019)
MARCH 06–08, 2019
6th BIOPESTICIDE INTERNATIONAL CONFERENCE (BIOCICON –
2019)
MARCH 06–08, 2019
INSECTICIDAL ROLE OF CUCURBITACIN ISOLATED FROM IBERIS AMARA L.
SIMMI SWARAJ, KUMARI TANVI, BIJAYA SAMAL, PRIYADARSHAN MISHRA, SMRUTI SUBHUDARSHINI,
SUMONA DAS MAHAPATRA, NIMMY SRIVASTAVA,
Amity University Jharkhand, 9955955186
INTRODUCTION
The cruciferae family is one of the largest plant family consisting of about 300
genera and 1500 species. It includes vegetable crops, garden and wild and
flowers and weeds (Rizk, 1986). The flea beetle Phyllotreta nemorum and Pieris
rapae are widespread and common pest of cruciferous plants.
Various control measures such as seed dressing with BHC or treatment with DDT,
BHC are in practice but Turnoc and Turnbill (1995) reported the development of
resistance by the insects towards insecticides. Further there are many problems
such as health hazards, environmental effects in the application of this insecticide.
Therefore it is necessary to search for alternative methods to control the beetle in
a ecofriendly manner.
MATERIALS AND METHODS
Iberis amara
!" ! # $% &
%!' (
Phyllotreta neumorum & Pieris rapae)
* "
)&%
+ ( ( " Phyllotreta
neumorum & Pieris rapae
,
RESULTS AND DISCUSSION
Table 1 show the population density of insect present per plant in both the varieties of
Bitter Candytuft recorded from year 2013-15. Cucurbitacin was detected in both varieties.
Maximum concentration of Cucurbitacin was estimated in compact head variety (0.54%)
as compared to Hyacinth flowered plant (0.52%). In both the varieties maximum content
of cucurbitacin was found in leaves than stems after 6 weeks of sowing(Table 2).
Table 1 Natural insect ( Phyllotreta nemorum and Pieris rapae) infestation observed in Iberis
amara flowering beds during the seasons of 2013 to 2015
CONCLUSIONS, RECOMMENDATION, AND FUTURE PROSPECTS
Sampling
date after
sowing
(week)
2013 2014 2015
Phyllotreta
nemorum
Pieris
rapae
Phyllotreta
nemorum
Pieris rapae Phyllotreta
nemorum
Pieris
rapae
2- - - - - -
6- - - - - -
10 - - - - - -
14 - - - - - -
18 - - - - - -
Table 2:- Secondary plant metabolites (Cucurbitacin &Glucosinolates) content and in leaves and stem of bitter Candytuft.Table 2:- Secondary plant metabolites (Cucurbitacin &Glucosinolates) content and in leaves and stem of bitter Candytuft.
Table 2:Secondary Plant Metabolites Cucurbitacin present in leaves and stem of two varieties
of Iberis amara L
Table 2:- Secondary plant metabolites (Cucurbitacin &Glucosinolates) content and in leaves and stem of bitter Candytuft.Table 2:- Secondary plant metabolites (Cucurbitacin &Glucosinolates) content and in leaves and stem of bitter Candytuft.
Compact head variety
Sampling date after sowing
(Weeks)
Total Cucurbitacin
Leaves
I II
Stem
I II
2 0.36 0.36 0.29 0.31
6 0.54 0.53 0.48 0.49
10 0.50 0.47 0.48 0.48
14 0.47 0.42 0.47 0.40
18 0.43 0.39 0.40 0.29
Mean 0.46 0.43 0.42 0.39
Hyacinth flowered variety
Sampling date after sowing
(Weeks)
Total Cucurbitacin
Leaves
I II
Stem
I II
2 0.38 0.37 0.30 0.30
6 0.52 0.51 0.48 0.50
10 0.49 0.47 0.51 0.50
14 0.47 0.45 0.43 0.47
18 0.39 0.40 0.41 0.42
Mean 0.45 0.44 0.42 0.43
The plant Iberis amara L. which is mainly known in India as an ornamental plants and grown in the gardens during the
winter season can be utilized as a bioinsecticide due to the presence of high concentration of Cucurbitacin E and I and
hence can be effectively control the pest Phyllotreta nemorum and Pieris rapae which cause a majot destruction to the
plants of cruciferae family