Himalayan (Himachal region) cedar wood (Cedrus deodara: Pinaceae) essential oil, its processing, ingredients and uses: A review

Abstract

EO's (Essential oils) are the complex mixtures of volatile compounds, synthesized as secondary metabolites from plant parts by steam/ Hydro distillation, solvent-less extraction and other physical means such as cold pressing. The demands of EO's are increasing drastically in food and beverage; fragrances; cosmetics; flavouring agents in air fresheners and deodorizers; household and aromatherapy. Cedrus deodara (Pinaceae) EO is one of the important oil, which has great pharmacological activities and demand in the market. C. Deodara EO has wide applications, mainly in antimicrobial, insecticidal, molluscicidal, germicidal, anti-tubercular activities in Pharmacology. C. Deodara oil has high demand in fragrance industries because of its specific characteristics. Himalayan C. Deodara consists of α-Himachalene, β-Himachalene, γ-Himachalene, α and γ atlantone as its major constituents. This paper presents the details of Cedrus deodara of Himachal (India) region, its production in small scale industries using steam/Hydro distillation, ingredient and uses. This article will facilitate the science students and small scale EO's industries to improve their productivity with the latest techniques.

Full text

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Journal of Pharmacognosy and Phytochemistry 2019; 8(1): 2228-2238
E-ISSN: 2278-4136
P-ISSN: 2349-8234
JPP 2019; 8(1): 2228-2238
Received: 19-11-2018
Accepted: 21-12-2018
Sandeep Kumar
Production Department, Shree
Khand Essential Oil Industry,
Mandi, Himachal Pradesh, India
Ashwani Kumar
Director, Manimahesh
Enterprises, Mandi, Himachal
Pradesh, India
Randhir Kumar
Project Manager, Civil
Department, Manimahesh
Enterprises, Mandi, Himachal
Pradesh, India
Correspondence
Sandeep Kumar
Production Department, Shree
Khand Essential Oil Industry,
Mandi, Himachal Pradesh, India
Himalayan (Himachal region) cedar wood (Cedrus
deodara: Pinaceae) essential oil, its processing,
ingredients and uses: A review
Sandeep Kumar, Ashwani Kumar and Randhir Kumar
Abstract
EO’s (Essential oils) are the complex mixtures of volatile compounds, synthesized as secondary
metabolites from plant parts by steam/ Hydro distillation, solvent-less extraction and other physical
means such as cold pressing. The demands of EO’s are increasing drastically in food and beverage;
fragrances; cosmetics; flavoring agents in air fresheners and deodorizers; household and aromatherapy.
Cedrus deodara (Pinaceae) EO is one of the important oil, which has great pharmacological activities
and demand in the market. C. Deodara EO has wide applications, mainly in antimicrobial, insecticidal,
mollusicidal, germicidal, anti-tubercular activities in Pharmacology. C. Deodara oil has high demand in
fragrance industries because of its specific characteristics. Himalayan C. Deodara consists of α-
Himachalene, β-Himachalene, γ-Himachalene, α and γ atlantone as its major constituents. This paper
presents the details of Cedrus deodara of Himachal (India) region, its production in small scale industries
using steam/Hydro distillation, ingredient and uses. This article will facilitate the science students and
small scale EO’s industries to improve their productivity with latest techniques.
Keywords: Essential oils (EO’s); Cedrus deodara (Pinaceae), processing, chemical constituents, recent
extraction techniques.
Introduction
Essential Oils (Aromatic oils) are synthesized as secondary metabolites from various parts of
plants and employed for flavors, perfumes, disinfectants, medicines and stabilizers. EO’s are
also utilized for making undesirable odors and as raw materials for making other products.
[CM, Cook et al 2016] [16]. The secondary metabolites are classified into terpenoids,
shikimates, polyketides and alkaloids. EO’s are mainly concerned with terpenoids and
shikimates. EO’s are also called volatile oils because of their ease of solubility in alcohol to
form essence. EO’s evaporates when exposed to air at ordinary temperature. EO’s are made up
of isoprene units (empirical formula C6H8) and are usually mono-terpenes (C5H8), sesqui-
terpenes (C10H16) and diterpenes (C15H20; C20H32) respectively. EO’s are classified into four
major categories: (a) Pinene or terpenes; (b) Oxygenated oils; (c) Nitrogenerated oils and, (d)
Sulfurated oils. Pinene and terpenes (e.g. Turpentine oil) contains carbon and hydrogen and
Empirical formula C10H16. Oxygenated EO’s (e.g. Cassia oil) contains carbon, hydrogen and
nitrogen. Nitrogenerated EO’s (e.g. Almond oil) contains carbon, hydrogen, oxygen and
nitrogen. Sulphurated EO’s (e.g. Mustard oils) contains carbon, hydrogen and sulphur. [et al]
EO’s are isolated by traditional technique mainly hydro/steam distillation from the leaves,
buds, flower, roots, seeds and other part of the plant.
Himalayan cedar (Cedrus deodara, deodar) and Sandlewood EO’s are distilled from the stem
and roots of the plants. Himalayan Cedrus deodara grows in the slope of the Himalayas and at
the elevation of 1650m to 2400m above the sea level. Cedrus deodara common name in
English as Himalayan cedar, deodar cedar and deodar and in the Hindi local name is Diar,
deodar and debdar. In Himachal Pradesh, the total 68,872 hectare area is covered under under
Cedrus deodara forest. Cedrus deodara is found in Chamba, Manali, Kinnaur, Sirmour,
Shimla, Kangra (Bada Bhangal & Chota bhangal) and Mandi region of Himachal Pradesh. The
height of Cedrus deodars is approximately 65-85 m tall and 4 m diameter at breast height
(DBH). The leaves of Cedrus deodara are stiff, sharp pointed having length of 23-37 mm long
and the bark is grayish brown, dark in color [Orwa et al, 2009]. The extracted oil from Cedrus
deodara have been used as antiseptic, insecticides, herbal remedies against animal diseases,
anti-fungal activity, anti-inflammatory, molluscidal activity, pharmacological and biological
activities, etc. The sizable stumps and roots of the plant left after cutting of trees are utilized
for the Hydro/Strem distillation of EO’s.
In India, the production of Cedrus deodara EO’s begun in late 1950’s and estimated to be

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Journal of Pharmacognosy and Phytochemistry
around 150 tonnes per annum of the world’s production.
[Coppen et al, 1995] [19]. The Cedrus deodara leaves are
utilized for flavoring foods, beverages and for the treatment of
diseases such as rheumatism, diabetes, cancer, stomach
disease, inflammation in tuberculous glands, etc. The wood
and bark extracts of Cedrus deodara are used for medicinal
purposes. The extracted root oil form Cedrus deodara is
utilized as an anti-ulcer drug. [Atwal et al, (1976); Zhang et
al, (2011); Krishnappa et al (1978); Mukherjee et al. (2011)]
[20, 21, 23, 24] The Himalayan Cedrus deodara plant species are
illustrated in Fig. 1. The taxonomical classification and
chemical composition of Cedrus deodar species is presented
in Table 1 and Table 2 respectively. The botanical and
common names of Cedrus deodara are given in Table 3.
Fig. 1: Cedrus Deodara plant (Himalayan deodar, Mandi region)
Table 1: Taxanomical Classification of Cedrus Deodara (Gupta et al, 2011) [7]
Taxonomical Classification (Cedrus deodara)
Division
Pinophyta
Kingdom
Plantae
Class
Pinopsida
Order
Pinales
Family
Pinaceae
Genus
Cedrus
Species
C. Deodara
Table 2: Cedrus deodara Plant Chemical Composition (Tewart LN et al, 2011) [25]
Chemical Composition in %age (Cedrus deodara)
Organic Carbon (C)
1. 83.50
Nitrogen (N)
2. 0.28
Phosphorus (P)
3. 0.055
Potassium (K)
4. 0.06
Calcium (Ca)
5. 2.60
Magnesium (Mg)
6. 0.017
Table 3: Cedrus Deodara botanical and common name (Orwa et al, 2009)
Botanical Name
Common Name
Trade Name
Cedrus deodara
In English: Himalayan cedar, deodar cedar, deodar
Diar, Deodar
In Hindi: Diar, deodar, debdar
In French: cedar del’himalaya
In German: himalaja-Zeder
In Italian: cedro dell’Himalaia
In Spanish: Cedro del Himalaya, cedro de la India
The characteristics of Cedrus deodara are illustrated in Table 4.
Table 4: Margin specifications
Species
Ripe color
Length (cm)
Width (cm)
Cedrus Deodara
Cone Size
Reddish brown
7813
589
Seed Size
Length (mm)
Width (mm)
104815
587
Leaf Size
Length (cm)
No. of Whorls

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Journal of Pharmacognosy and Phytochemistry
Fig. 2: Plant organs containing EO’s
Fig. 3: Chemical groups present in EO’s

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Journal of Pharmacognosy and Phytochemistry
Fig. 4: Steam Distillation Plant arrangement and Instrumentation. [K. Tuley DE SILVA, 1995]
2. Processing of Cedrus deodara
The stumps and roots of the plants (left after cutting) are
collected from the forest areas. The wood is reduced into
small pieces and subjected to the continuous Hydro/Steam
distillation process in Industries. In small scale industries,
traditional Hydro/steam distillation process is employed for
the processing of Cedrus deodara. Before starting of hydro
distillation process, Cedrus deodara wood is chopped into
small pieces. These pieces are processed through a grinder to
convert chips into powder form. This powder material is filled
into the still or containers where the plant material rests on a
perforated tray for quick removal after extraction. The
schematic of the steam distillation process is illustrated in
Figure 5.

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Journal of Pharmacognosy and Phytochemistry
Fig. 5: Schematic of processing of Cedrus deodara using
Hydro/Steam distillation process
In the Steam distillation process, the steam is produced in a
steam boiler and the generated steam is blown through a pipe
into the bottom of the still. 70-75 psi pressure is maintained in
the boiler. High-pressure steam distillation is often applied
and the time of distillation the applied increased temperature
significantly reduces. The steam carries volatile oil
constituents along with it and passes through a condenser. The
condenser converts the vapors into liquid and the condensed
distillate consists a mixture of water and oil. The mixture of
oil and water is separated in a Florentine flask or receptacle
made of SS (stainless steel) whose one outlet near the base
and another at the top from which oil and water can be
separately withdrawn. Since the density of Cedrus deodara
EO’s is lighter than the water, therefore, the oil is collected
from the top end of the Florentine flask or receptacle.
For large scale production of Cedrus deodara EO’s, this
method is most widely preferred. For higher yield of EO’s,
the processing time approx. 8 to 9 hours is preferred. After
distillation, the crude oil is stored in plastic drums. The
Cedrus deodara EO sample obtained after Hydro/Steam
distillation is shown in Figure 6.
Fig. 6: Sample of Cedrus deodara Essential Oil
Table 5: Physical properties of Cedrus Deodara oil [Walker (1968);
Adams (1991)] [1]
Physical properties (Cedrus deodara oil)
Specific gravity at 15ᵒC (or 20ᵒC)
0.94-0.99
Optical rotation
-16ᵒ to -60ᵒ
Refractive Index at 20ᵒC
1.48-1.51
Solubility (at 20ᵒC)
90%-95% in ethanol
3. Various parameters affecting Yield & Quality of C.
Deodara
During steam distillation, the various factors such as steam
pressure and temperature, equipment’s materials etc. affects
the quality and yield of C. Deodara which may lead to change
the characteristics of distillation. To avoid these distillation
characteristic changes, the list of major factors should be
taken into consideration during steam/Hydro distillation are as
follows:
- Distillation mode (Steam pressure and temperature)
- Design of the equipments
a.) Design of still, condenser and separator
b.) Design of furnace and chimney
c.) Ratio of tank Height and diameter of cylinder
- Fabrication and Materials of equipments
- Raw material condition
- Distillation time
- Loading and unloading of raw material
- Condition of still and water tank
4. Purification of Cedrus deodara Crude oil
Cedrus deodara crude oil is obtained from the oil separator
after steam distillation. It contains moisture content and
impurities in it which degrades the characteristics of C.
Deodara. The impurities and moisture accelerate the
undesirable chemical reaction and polymerization.
The high speed centrifugation process is most commonly used
to remove the impurities and moisture contents. Another
method is the addition of anhydrous sodium sulphate in the oil
and standing it for overnight and filtered help to remove the
impurity and moisture.
EO’s are frequently rectified or re-distilled to remove
impurities. The steam distillation process is carried out under
vacuum condition by keeping the temperature within limits.
5. Chemical constituents of Cedrus deodara
Cedrus deodara EO is utilized as perfume fixative in essence,
cosmetic, soap and perfume Industries. Generally, the Cedrus
deodara is characterized by a high percentage of
himachalenes [Bhushan, et al, (2006)]. EO enriched in
atlantones is known as “Perfumery Grade” and the EO
enriched with himachalene is called as “Super Rectified Oil”.
Sesquiterpenes are partially being ascribed from Cedrus
species.
Abha Chaudhary et al. (2011) [9] investigated and
characterized the constituents of Cedrus deodara for the use
of pest management. The authors identified the fractions of
forty components using GC and GS-MS analysis. In Cedrus
deodara, the major constituents includes α-Himachalene, β-
Himachalene, γ-Himachalene, (Z)-γ-atlantone, (E)-γ-
atlantone, (Z)-α-atlantone and (E)-α-atlantones. The identified
chemical constituents of Cedrus Deodara by Chaudhary Abha
et al, 2011 [9] are shown in Fig. 7 where A5 represents:
Pentane Fraction; A4: Himachalene enriched fraction; A3:
Atlantone enriched fraction; A6 : Acetonitrile Fraction and
A2 represents Crude Oil.

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Journal of Pharmacognosy and Phytochemistry
The sesquiterpenes and their chemical structure available in
Cedrus deodara are presented in Figure 8 as himachalenes (α,
β and γ) (1-3), isocentdarol (4), Himachalol (5),
allohimachalol (6), aryl Himachalene (7), (E)-α-atlantone (8),
(E)-γ-atlantone (9), (Z)-α-atlantone (10), (Z)-γ-atlantone (11),
deodarone (12), oxidohmachalene (13), β-himachalene
monoepoxide (14), atlantolone (15), deodardione (16),
diosphenol (17), limonene carboxylic acid (18). (Nigam et al.
1990, Bhushan, et al 2006) [25, 26]
Fig. 7: Chemical constituents of fraction of Cedrus deodara EO [Chaudhary A. et al. (2011)] [9]

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Journal of Pharmacognosy and Phytochemistry
Fig. 8: Structure of constituents of Cedrus deodara
The chemical constituents (through ethanolic extract) found in
needles of Cedrus deodara are taxifolin (19), quercetin (25),
myricetin (45) 2R, 3R-dihydromyricetin (46), cedrusone A
(47), β-sitosterol (48), 1-[3-(4-hydroxyphenyl)-2-propenoate]-
α-D-glucopyranoside (49),
10-nonacosanol (50), dibutyl phthalate (51), phthalic acid bis-
(2-ethylhexyl)ester (52), protocatechuic acid (53), Shikimic
acid (54) and 5p-trans-coumaroylquinic acid (55). [Zhang et
al. (2011); Liu et al. (2011)] Srinivastava et al 2001 [21, 27, 30]
reported the Centdaroic acid (56) generally known as
diterpene in Cedrus deodara roots, presented in Figure 9.

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Journal of Pharmacognosy and Phytochemistry
Fig. 9: Structure of the constituents of Cedrus deodara root
diterpene Centdaroic acid (56)
4. Modern Extraction methods for EO’s
Now days, it becomes easier to extract the EO’s because of
the continuous improvement and innovation in technologies.
The green technologies have been invented which leads to:
- the decrease in extraction time;
- Reduction in solvent involvement;
- Reduction in the interaction of volatile liquid with
atmospheric conditions and
- Reduction in energy.
Some of the solvent-less extraction processes are carried out
in a vacuum under reduced pressure to improve the yield and
quality of EO’s. [Muhammad Shahzad Aslam et al, 2016] [10]
The lists of some solvent-less extraction methods suggested
by Aslam (2016) [10] are as follow;
a.) SFMS (Solvent-free microwave extraction)
b.) MHG (Microwave Hydro-diffusion and gravity)
c.) Improved solvent free microwane extraction.
The advantages of these extraction methods are as follows:
- Fast action
- Cleanliness
- Low energy output
- Green method
The schematic of these solvent-less extraction processes are
shown in Fig. 10-12.
Fig. 10: Schematic of SFME [Kusuma H. et al, 2016] [12]
Fig. 11: Schematic of MHG [Li Y, et al, 2013] [14]
Fig. 12: Schematic of Improved SFME [Wang T. et al, 2006] [11]
d.) CO2 Extraction method
Subcritical and supercritical liquid CO2 extraction is another
modern technique employed for extraction of essential EO’s.
The EO is extracted with liquid CO2 in an autoclave at
temperature range of 50-70ᵒC. After that the gas is distilled
and pure extract remains. The extracted product through this
method remains stable for long storage and more complete.
For medical and biological purposes, subcritical extraction is
preferred. The amount of pure extracts (Oil-soluble) from the
needles of Siberian cedar with the use of subcritical extraction
is about 2%. During sub-critical extraction, there is practically
no destruction of the chemical structure of the components.
The biological activity of such drugs is very high. The
schematic of liquid CO2 extraction is illustrated in Fig. 13.
Fig. 13: Schematic diagram of the supercritical CO2 extraction. A:
Pumping section; B:Extraction section; C1 and C2: Fractional
section; D: Flow measurement section [E. Reverchon et al, 1992] [6]

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Journal of Pharmacognosy and Phytochemistry
e.) Modern (Non-traditional) methods of extraction of
essential oils
 Headspace trapping techniques
 Solid phase micro-extraction (SPME)
 Supercritical fluid extraction (SFE)
 Phytosol (phytol) extraction
 Protoplast technique
 Simultaneous distillation extraction (SDE)
 Controlled instantaneous decomposition (CID)
 Thermomicrodistillation
 Micro distillation
 Modular spinning band distillation
 Membrane extraction
5. List of equipments required for Hydro/Steam
distillation Small-scale plant
The major machinery and equipments required for the
establishment of small-scale Hydro/Steam distillation EO’s
plant are as follows;
- Band saw (for wood cutting purposes)
- Chipper (to cut the wood into small chips)
- Grinder (to convert the chips into powder form)
- Steam Boiler (for stean generation)
- Still (to fill powder material)
- Condenser (to condense the mixture of water and volatile
oil)
- Florentine flask
For the testing and characterization of EO’s, the following
lists of equipments are required in the laboratory;
- Laboratory balance (to determine the weight of plant
samples)
- Clevenger apparatus (for essential oil extraction and
estimation of EO’s percentage)
- Freezer (to keep extracted volatile oils)
- Gas chromatography- Mass Spectroscopy (for the
determination of essential oil components)
- Refractometer (to determine the refractive index of
essential oils).
6. Applications of C. Deodara Essential oil
 Fragrance Compounding
 Source of raw components for the production of
additional fragrance compounds.
 Scented soaps
 Room sprays
 Disinfectants
 Cleaning agent for microscope sections
 Technical preparation
 All branches of medicines such as in pharmacy,
balneology, massage and homeopathy
 In the field of aromatherapy and aromachology
 Biocides and insect repellents and many more.
7. Market for EO’s
In 2017, the worldwide production of EO’s was estimated as
1,50,000 tonnes i.e. triple in volume since 1990’s.[Cinzia
Barbieri et al, 2018] [8]. Therefore the demands of EO’s are
increasing drastically in fragrance industries (29%),
household (16%), food and breverage (35%), cosmetics,
aromatherapy and pharmaceuticals (15%) [Data as per
Federation of EO’s]. The demands of EO’s, major leading
importers and exporters are shown in Fig. 14, Fig. 15 and Fig.
16 respectively.
Fig. 14: World processing of EO’s, [Cinzia Barbieri et al, 2018] [8]
Fig. 15: Leading Importers of EO’s, (2016, % volume) [Cinzia
Barbieri et al, 2018] [8]
Fig. 16: Leading Exporters of EO’s, (2016, % volume) [Cinzia
Barbieri et al, 2018] [8]
References
1. Adams RP. Modern methods of Plant Analysis,
Germany: Springer-Verlag Berlin Heidelberg, 1991, 12.
2. Adams RP. Investigation of Juniperus species of the
United States for new sourses of cedar wood oil, Econ
Bot. 1987; 41:48-54.
3. Adams RP, Granat M, Hogge LR, Von Rodloff E.
“Identification of lower terpenoids from gas-
chromatography-mass spectral data by on-line computer
method, J Chromatogr Sci. 1979; 17:75-81.
4. Kitchens GC, Dorsky J, Kaiser K. “Cedar wood oil and
derivatives Givaudanian. 1971; 1:3-9.

~ 2238 ~
Journal of Pharmacognosy and Phytochemistry
5. Runeberg J. The chemistry of the order Cupressales,
Heartwood constituents of Juniperus Cedrus Webb. &
Benth, Acta Chem Scand. 1960; 14:991-1994.
6. E Reverchon, F Senatore. Isolation of Rosemary Oil:
Comparision between Hydrodistillation and Supercritical
CO2 Extraction, Flavour and fragrance Journal. 1992;
7:227-230.
7. Sumeet Gupta, Anu Walia, Rajat Malan. “Phytochemistry
and Pharmacology of Cedrus Deodara: An overview”,
International Journal of Pharmaceutical Sciences and
Research. 2011; 2(8):2010-2020.
8. Cinzia Barbieri, Patrizia Borsotto. Potential of Essential
Oil: Market and Legislation,
doi: 10.5772/intechopen.77725.
9. Abha Chaudhary, Prabha Sharma, Gireesh Nadda,
Dhananjay Kumar Tewary, Bikram Singh. “Chemical
composition and larvicidal activities of the Himalayan
cedar, Cedrus deodara essential oil and its fractions
against the diamondback moth, Plutella xylostella”,
Journal of Insect Science. 2011; 11(157):1-10. doi:
10.1673/031.011.15701.
10. Muhammad Shahzad Aslam, Muhammad Syarhabil
Ahmand, Sarwat Ali Raja. Green Chemistry: Solventless
Extraction of Essential Oil, Intechopen Publication, 39-
48 doi: 10.5772/intechopen.72401.
11. Wang Z et al. Improved solvent-free microwave
extraction of essential oil from dried Cuminum cyminum
L. and Zanthoxylum bungeanum maxim. Journal of
Chromatography. A. Jan. 2006 ;1102(1-2):11-17
12. Kusuma H, Putri D, Dewi I, Mahfud M. Solvent-free
microwave extraction as the useful tool for extraction of
edible essential oils. Chemistry & Chemical Technology.
2016; 10(2):213-218.
13. Li XJ, Solvent-free microwave extraction of essential oil
from Dryopteris fragrans and evaluation of antioxidant
activity. Food Chemistry. 2012; 133(2):437-444.
14. Li Y, Fabiano-Tixier AS, Vian MA, Chemat F. Solvent-
free microwave extraction of bioactive compounds
provides a tool for green analytical chemistry. Tr AC,
Trends in Analytical Chemistry. 2013; 47:1-11.
15. Huai-Qiong Chen, Jian-He Wei, Jun-Shan Yang, Zheng
Zhang, Yun Yang, Zhi-Hui Gao and Bao Gong, Chemical
condstituents of Agarwood Originationg from the
Endemic Genus Aquilaria Plants, Chemistry &
Biodiversity. 2012; 9:236-250.
16. Cook CM, Lanaras T. Essential Oils: Isolation,
Production and Uses. In: Caballero, B., Finglas, P., and
Toldrá, F. (eds.) the Encyclopedia of Food and Health.
2016; 2:552-557. Oxford: Academic Press.
17. Robert S Pappas, Sylla Sheppard-Hanger. “Essential Oil
of Eucalyptus camaldulensis Dehn. From South Florida:
A High Cryptone/Low Cineole Eucalyptus”, Journal of
Essential Oil Research. 2000; 12:383-384.
18. K. Husnu Can Baser, Gerhard Buchbauer. “Handbook of
Essential Oils Science, Technology and Applications”,
2010 CRC Press, Taylor and Francis Group, Boca Raton,
Fl 33487-2742.
19. Coppen JJW. Cedarwood Oils In: Flavours and
Fragrances of Plant Origin. Food and Agriculture
Organization of the United Nations, 1995.
20. Atwal AS. Agricultural pests of India and South East
Asia. Kalyani Publishers, Ludhiana, India, 1976.
21. Zhang JM, Shi XF, Ma QH, He FJ, Fan B, Wang DD et
al. Chemical constituents from pine needles of Cedrus
deodara. Chemistry of Natural Compounds. 2011; 47:
272-74.
22. Fleisher A. The volatiles of the leaves and wood of
Lebanon Cedar (Cedrus libani A. Rich) aromatic plants
of the Holy Land and the Sinai. Journal of Essential Oil
Research. 2000; 12:763-65.
23. Krishnappa S, Dev S. Studies in sesquiterpenes-LVIII:
Deodardione, a sesquiterpene diosphenol and, limonene
carboxylic acid, a possible norses quitter pene
compounds from the wood of Cedrus deodara Loud.
Tetrahedron. 1978; 34:599 -602.
24. Mukherjee PK. Quality Control of Herbal products. 2001,
492-515. Business Horizons, New Delhi.
25. Nigam MC, Ahmad A, Misra LN. Composition of the
essential oil of Cedrus deodara. Indian Perfumer. 1990;
34:278-81.
26. Bhushan S, Singh J, Rao JM, Saxena AK, Qazi GN. A
novel lignin composition from Cedrus deodara induces
apoptosis and early nitric oxide generation in human
leukemia Molt-4 and HL-60 cells. Nitric Oxide. 2006;
14:72-88.
27. Liu S, Tian X, Chen X, Hu Z. Separation and
determination of Podophyllum lignans by micellar
electrokinetic chromatography. Journal of
Chromatography A. 2001; 928:109-15.
28. Chatterjee A, Prakarashi SC. Treatise of Indian Medicinal
Plants. Coun. Sci. Industr. Res. New Delhi, 1991.
29. Tiwari DM. Distribution and Morphology: A monograph
on Deodar. International book Distributors, Dehradun,
1994, 14.
30. Tiwari AK, Srinivas PK, Kumar SP, Rao JM. Free
Radical Scavenging Active Components from Cedrus
deodara. J Agric Food Chem. 2001; 49: 4642-4645.
31. Kar K, Puri VN, Patnaik GK, Sur RN, Dhawan BN,
Kulshrestha DK, Rastogi RP. Spasmolytic constituents of
Cedrus deodara (Roxb.) Loud: Pharmacological
evaluation of himachalol. J Pharm Sci. 1975; 64:258-262.
32. Agarwal PK, Rastogi RP. Lignans from Cedrus deodara.
Phytochemistry. 1982; 21:1459-1461.
33. Shankarnarayan R, Krishnappa S, Sukh Dev. Studies in
sesquiterpenes-L11: Isohimachalone a compound of
essential oil from wood of Cedar deodara. Tetrahedron.
1997; 33:885-886.
34. Chopra AK, Gupta V, Gupta KK, Prasad G. Antibacterial
activity of root, stem and leaf extract of Cedrus deodara
against Escherichia coli in vitro. Flora and Fauna. 2004;
2:101-103.
35. Patel RB. Antibacterial evaluation of ethanolic extract of
Cedrus deodara wood. Arch Appi Sci Res. 2010; 2:179-
183.
36. Essien EP, Essien JP. Control of fungal deterioration of
two varieties of Capsicum annuum during storage by the
essential oil of Cedrus deodara. Nig J Natl Pdts Med.
2000; 4:62-64.
37. Singh D, Aggarwal SK. Insecticidal principles of
Himalayan Cedar Wood Oil. Journal of Chemical
Ecology. 1988; 14:1145-1151.
38. Agarwal PK, Rastogi RP. Terpenoids from Cedrus
deodara. Phytochemistry. 1981; 20:1391-1321.
39. Pandey S, Devmurari V, Goyani M, S Vaghani, K
Jaganathan. Formulation and Evaluation of Cedrus deodara
Loud Extract. Int J Chem Tech Res. 2009; 1:1145-1152.
40. Tewary DK, Bhardwaj A, Shanker A. Pesticidal activities in
five medicinal plants collected from mid-hills of western
Himalayas. Industrial Crops and Products. 2005; 22:241-
247.