This study presents the formulation of nanostructured lipid carrier encapsulated Zingiber zerumbet oil (NLC-ZZ) using ultrasonication technique. NLC is the blend of solid lipid, liquid lipid and surfactant for encapsulation of poor water soluble actives. The NLC-ZZ formulation was characterized with respect to particle size, polydispersity index (PDI), zeta-potential, encapsulation efficiency and physical morphology. The NLC-ZZ formulation had an average diameter of 96.59 nm, PDI of 0.192, zeta-potential of-39.88 mV, and encapsulation efficiency of 90%, respectively. The NLC formulation for Zingiber zerumbet oil encapsulation has been successfully developed and is suitable for transdermal delivery system due to their nano-size and stability.