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Abstract
Objective In order to understand the genetic diversity of cultivated and wild Rehmannia glutinosa, and to unravel the origin of cultivated R. glutinosa. Methods The sequences of nuclear gene ITS and chloroplast gene psbA-trnH, trnS-trnG in cultivar and wild population of R. glutinosa were amplified and sequenced. Haplotype (gene) diversity and nucleotide polymorphism of three genes from wild and cultivars of R. glutinosa were analyzed and compared in this study. Phylogenetic tree was constructed based on combined three genes using Bayesian inference (BI) methods. Results Analysis of sequences indicated that the number of haplotype (ITS, 6; psbA-trnH, 8; trnS-trnG, 9) in wild population of R. glutinosa was obviously higher than the number of Haplotype (ITS, 3; psbA-trnH, 3; trnS-trnG, 3) in cultivars of R. glutinosa. Haplotype diversity and nucleotide polymorphism of wild population of R. glutinosa were far higher than that of cultivars of R. glutinosa. The NJ tree (combined three genes data) indicated that all cultivated and wild population of R. glutinosa, and R. solanifolia form a monophyletic clade [Posterior probability (PP) = 90%]. Twenty-three cultivars of R.glutinosa (including 29 samples) were clustered with Wenxian wild populations (PP = 78%). Conclusion The results implied very low genetic diversity existed in cultivars of R. glutinosa induced by the severe genetic bottleneck during the process of domestication of wild R. glutinosa, which resulted in the narrow genetic basis of the existing cultivars and decreased genetic diversity. Furthermore, it appeared that wild populations in Wenxian-Henan area were involved in the origin of cultivars of R. glutinosa. © 2018, Editorial Office of Chinese Traditional and Herbal Drugs. All right reserved.
... Although the complete chloroplast (cp) genome of six species in Rehmannia is reported by Zeng et al. (2017), the cultivar of R. glutinosa in its geo-authentic distribution is unknown. The cultivar of R. glutinosa was proved to derive from the wild population (Xia et al. 2018). Dense and hypertrophic leaves and expansion tuberous root are the main domestication trait of R. glutinosa cultivar Wen 85-5 ( Figure 1(A)) which is different from wild population (Figure 1(B)). ...
The cultivar of Rehmannia glutinosa (Orobanchaceae) is one of the four famous ‘Huai’ medicine cultivar species endemic to Henan Province in central China. In this study, we report the complete chloroplast (cp) genome of R. glutinosa cultivar Wen 85-5. The cp genome of R. glutinosa cultivar Wen 85-5 was 155,499 bp in length and contained a pair of inverted repeat regions (IR, 25,748 bp) separated by a small single copy (SSC, 17,600 bp) and a large single copy (LSC, 84,403 bp) region. Chloroplast genome sequences of two cultivar of R. glutinosa (Wen 85-5 and Jiwang 1) are identical to each other. The sequence of cp genome of R. glutinosa cultivar Wen 85-5 was 99.70% similar to the wild population of R. glutinosa. Some distinctive insert and deletion in R. glutinosa cultivar Wen 85-5 by comparison with wild population were reported. The maximum-likelihood phylogenetic analysis revealed that R. glutinosa cultivar Wen 85-5.was sister to the R. glutinosa cultivar Jiwang 1 (BS = 100%), and further clustering with R. glutinosa (BS = 100%). This result will be helpful for the conservation and breeding programs of the cultivar of R. glutinosa.
... The sequences of nuclear gene ITS and chloroplast psbA-trnH and trnS-trnG in cultivars and wild populations of Rehmannia glutinosa were amplified and sequenced. The haplotype (gene) diversity and nucleotide polymorphism of three genes from wild plants and cultivars of R. glutinosa were analyzed and compared ( Xia, Huang, Li, Zhou, & Gao, 2018 ). ...
In recent years, the development of Chinese herbal medicine (CHM) has been challenged by shortages of CHM resources and drug safety concerns related to end products. There have been significant efforts by Chinese scholars to tackle these challenges, which are revealed by analyzing the research trend of CHM resources via surveying Chinese Traditional and Herbal Drugs (Zhong Cao Yao), a representative journal in CHM. Our study focused on 781 articles in CHM resources from 2013 to 2018 and included four subject areas: germplasm resources, quality analysis and evaluation, cultivation, and bioengineering of CHM. Discussion and prospective for future investigations were also presented, including: construct the core germplasm of medicinal plants and expand germplasms; combine molecular research with field experiments and promote the deeper study of cultivation of CHM plants; improve the quality evaluation method of CHM and strengthen the identification of Chinese patented medicines; promote the sustainable development of CHM resources by utilizing bioengineering and synthetic biology. This study helps international scholars understand the status quo of CHM research and provides theoretical support for the healthy, modern, and international development of CHM, and it will facilitate the sustainable development of the traditional Chinese medicine industry.
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