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Zamioculcas zamiifolia is an ornamental plant that had been used pharmacologycally as a traditional medicine plant. The aims of this research were to investigate the phytochemistry of n-hexane, chloroform and methanol extracts from the roots of Z. zamiifolia and to evaluate their biological activities as antioxidant and cytotoxic. All extracts were screened phytochemically using specific reagents and TLC technique to reveal the major component of each extract. The chloroform was obtained as the highest quantity of extract which gave positive test for steroids and flavonoids. All extracts were tested for their antioxidant activity using DPPH method and for their cytotoxic activity against two types of human cancer cell lines, HepG2 and T47D, and normal African green monkey kidney ephitelial cell lines, Vero. The methanol and chloroform extract exhibited antioxidant activity with AA50 values of 180.0 and 431.5 µg/mL, respectively. Chloroform and methanol extracts showed potencial and moderate cytotoxicity towards T47D cell line with IC50 433.1 and 461.1µg/mL, respectively. None of the extracts was toxic against HepG2 and Vero cell lines.
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Rini Muharini, et. al. / Indo. J.Pure App. Chem. 1 (2), pp. 62-67, 2018
Indonesian Journal of Pure and Applied Chemistry
journal homepage: http://jurnal.untan.ac.id/index.php/IJoPAC
Indo. J.Pure App. Chem. 1 (2), pp. 62-67, 2018
PHYTOCHEMICAL SCREENING, ANTIOXIDANT, AND CYTOTOXICITY
OF Zamioculcas zamiifolia ROOT EXTRACT
Rini Muharini1*, Masriani1, Rudiyansyah2
1Department of Chemistry Education, Faculty of Teacher Training and Education, Tanjungpura
University, Jl. Prof. H. Hadari Nawawi, Pontianak, 74014, Indonesia
2Department of Chemistry, Faculty of Mathematics and Sciences, Tanjungpura University, Jl. Prof. Dr.
H. Hadari Nawawi, Pontianak, 74014, Indonesia
*Corresponding author: rini.muharini@chem.edu.untan.ac.id
ARTICLE INFO
ABSTRACT
Article history:
Received 07 July 2018
Accepted 19 December
2018
Available online
30 December 2018
Keywords:
phytochemical
screening, anti-
oxidant, cytotoxicity,
Zamioculcas zamiifolia
Zamioculcas zamiifolia is an ornamental plant that had been used
pharmacologycally as a traditional medicine plant. The aims of this
research were to investigate the phytochemistry of n-hexane,
chloroform and methanol extracts from the roots of Z. zamiifolia and
to evaluate their biological activities as antioxidant and cytotoxic. All
extracts were screened phytochemically using specific reagents and
TLC technique to reveal the major component of each extract. The
chloroform was obtained as the highest quantity of extract which
gave positive test for steroids and flavonoids. All extracts were tested
for their antioxidant activity using DPPH method and for their
cytotoxic activity against two types of human cancer cell lines, HepG2
and T47D, and normal African green monkey kidney ephitelial cell
lines, Vero. The methanol and chloroform extract exhibited
antioxidant activity with AA50 values of 180.0 and 431.5 µg/mL,
respectively. Chloroform and methanol extracts showed potencial and
moderate cytotoxicity towards T47D cell line with IC50 433.1 and
461.1µg/mL, respectively. None of the extracts was toxic against
HepG2 and Vero cell lines. © 2018 IJoPAC. All rights reserved
1. Introduction
Zamioculcas zamiifolia is widely known by Indonesian as ornamental plants, with local name
Dolar plants. Z. zamiifolia plant is an Araceae family, that is known for its toxicity [1],[2]. With
regard to their close relationship, Z, zamiifolia were assumed to be toxic as well. Literature study
showed that the leaves of Z. zamiifolia are able to absorb the volatile organic compounds, such as
benzene, toluene, ethylbenzene, and xylene from air [3],[4]. These facts concern people who utilize
this plant as an ornamental plant.
This plant has been utilized externally as traditional medicine by some local people from
other parts of the globe. Malawian people use the leaves of Z. zamiifolia as earache on children,
meanwhile the roots are used for treating gastric problems by Sukuma people in Tanzania.
Previously, the research group of Le Moullec (2015) reported that the leaf extract of Z.zamiifolia
did not show cytotoxicity against Artemia salina, using brine shrimp lethality (BSL) method [5].
This latest result dissmissed the assumption of the toxicity of Z.zamiifolia leaves. Moreover, Le
Moullec.(2015) obtained secondary metabolites from the leaves extract of Z. zamiifolia,
Rini Muharini, et. al. / Indo. J.Pure App. Chem. 1 (2), pp. 62-67, 2018
Rini Muharini, et. al. / Indo. J.Pure App. Chem. 1 (2), pp. 62-67, 2018
Extract
Weight
(gram)
Rendement (%) a
n-hexane
1.80
0.87
chloroform
3.58
1.74
methanol
0.65
0.32
Rini Muharini, et. al. / Indo. J.Pure App. Chem. 1 (2), pp. 62-67, 2018
Specific Reagent
n-hexane
extract
Chloroform
extract
Methanol
extract
Liebermann Burchard
+
-
+
Salwskosky
+
+
-
Shinoda
-
++
++
FeCl3
-
-
++
Mayer
-
-
-
Dragendorff
-
-
-
Extract
Anti-oxidant
activity AA50 (ppm)
Cytotoxicity (IC50)
Selectivity
Index (SI)
HepG2
(µg/mL)
T47D
(µg/mL)
Vero
(µg/mL)
HepG2
T47D
n-hexane
dn*
dn*
dn*
dn*
-
-
chloroform
431.5
3801.89
562.34
4677.35
1.22
8.32
methanol
180.1
2818.38
645.65
1047.13
0.37
1.62
Rini Muharini, et. al. / Indo. J.Pure App. Chem. 1 (2), pp. 62-67, 2018
0
20
40
60
80
100
120
0.0 1.0 2.0 3.0
Viability of cell (%)
Log concentration (μg/mL)
Cytotoxicity of MeOH Extract
HepG2
T47D
Vero
0
20
40
60
80
100
120
0.0 1.0 2.0 3.0
Viability of cell (%)
Log concentration (μg/mL)
Cytotoxicity of CHCl3Extract
HepG2
T47D
Vero
Rini Muharini, et. al. / Indo. J.Pure App. Chem. 1 (2), pp. 62-67, 2018
... Esta especie, posee un amplio uso para la ambientación de interiores (Chen, Henny & McConnell, 2002) y es reconocida su amplia aceptación por cultivadores y compradores (Chen & Henry, 2003). Se conocen además algunos estudios sobre sus propiedades para mejorar la calidad del aire (Zhou, Qin, Su, Liao & Xu, 2011) y sus efectos citotóxicos y antioxidantes (Muharini, Masriani & Rudiyansyah, 2018). ...
... Además de la utilidad ornamental, ya señalada, también han sido estudiadas sus potencialidades para la ambientación de espacios interiores y su asociación a la práctica del feng shui (Chen, Henny & McConnell, 2002). No se tienen referencias de su empleo en la alimentación de animales de cría o humanos, aunque resultaría beneficioso investigar al respecto, dado su valor como antioxidante (Muharini, Masriani & Rudiyansyah, 2018 En el campo de la obtención de bioactivos con fines médicos, están documentadas promisorias aplicaciones en la farmacología oncológica de los extractos de la raíz de esta planta, dados sus efectos antioxidantes y citotóxicos observados experimentalmente (Muharini, Masriani & Rudiyansyah, 2018). ...
... Además de la utilidad ornamental, ya señalada, también han sido estudiadas sus potencialidades para la ambientación de espacios interiores y su asociación a la práctica del feng shui (Chen, Henny & McConnell, 2002). No se tienen referencias de su empleo en la alimentación de animales de cría o humanos, aunque resultaría beneficioso investigar al respecto, dado su valor como antioxidante (Muharini, Masriani & Rudiyansyah, 2018 En el campo de la obtención de bioactivos con fines médicos, están documentadas promisorias aplicaciones en la farmacología oncológica de los extractos de la raíz de esta planta, dados sus efectos antioxidantes y citotóxicos observados experimentalmente (Muharini, Masriani & Rudiyansyah, 2018). ...
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