Content uploaded by Shobha Govindaiah
Author content
All content in this area was uploaded by Shobha Govindaiah on Jan 17, 2019
Content may be subject to copyright.
Vinutha Moses et al, J Adv Sci Res, 2014, 5(4): 01-03 1
Journal of Advanced Scientific Research, 2014, 5(4)
Journal of Advanced Scientific Research
Available online through http://www.sciensage.info/jasr
STUDY ON ADVANCED APPLICATION OF MINT OIL
Nethravathi Mahadevappa, Divya Kittayanapallya Hanumantharaju Pooja Vishwanath Suvarna, Vinutha Moses*,
Soumya Chandrashekar and Shobha Gowda
Department of Biotechnology, Sapthagiri College of Engineering, Bangalore-57
*Corresponding author: vinuthamoseschetan@sapthagiri.edu.in
ABSTRACT
Peppermint is most popular in traditional medicines such as herbal tea and essential oil. It is a cultivated natural
hybrid of Mentha aquatic L. (watermint) and Mentha spicata L. (spear mint) in many parts of the world.
It is extracted by steam distillation and shows potential actions like antioxidant, antiallergic, antimicrobial, antiviral, antimycotic, an
titoxigenic, antifungal, antiparasitical, anti-inflammatory, antiseptic, insecticidal and antitumor. The unique property of mint is
analgesic (pain-killing). These properties contribute to its applications and hence it is widely used in pharmaceutical, food
industries, agricultural field and is also effective on gastrointestinal tissue, respiratory system, central and peripheral nervous
system. This study focuses on recent advances and application of mint oil.
Keywords: Peppermint, essential oils, antimicrobial activity, mint, production, analgesic, anti- inflammatory
1. INTRODUCTION
Herbs are a boon to traditional medicine practiced from
ancient times. Plants are a potential source of traditional
ayurvedic medicine, either in its natural form or processed have
been used in curing many ailments. It provides a wide range of
bioactive compounds present in different parts of plants that can
be used for alternate therapy [1].There are different plants of
medicinal value but our interest lies on peppermint or
commonly known as mint Leaves.
1.1. Mint Leaves
Carl Linnaeus was renowned for first describing
peppermint from plants and leaves in England during 1753 [2].
Mentha is derived from Greek word mintha [3], very commonly
known as mint or peppermint. They are rarely yearly herbs
aromatic and perennial [4]. It has almost 25 perennial species,
belongs to the genus Mentha of the Lamiaceae family [5]. Mint
leaf is as shown in fig. 1.
Fig. 1: Mint Leaves1
It is distributed wildly and can be seen in almost all
environmental conditions especially wet and moist soil [6]. It
can also sustain sun heat and grows well even at this condition
[7]. Table1 shows its taxanomy.
In England, till 18th century the hybridization and cultivation
of peppermint was not done. It was only cultivated during 1500
BC being considered as a hybrid of water mint and spearmint
and thereafter it was used in cooking [8]. Before 2000 BC mint
has been used for medicinal purposes, but until 1771 menthol
was not isolated [9]. Rosmarinic acid, several flavonoids,
primarily eriocitrin, luteolin and hesperidin are the phenolic
constituents of peppermint leaves [10]. Menthol, menthone and
cineol are the main volatile components present. Mint does not
produce seeds being a perennial plant [11].
Table1. Peppermint Taxanomy [3]
1.2. Mint Oil
When the mint leaves are crushed, ground and steam
distilled, the volatile distillate thus obtained is said to be mint
oil [12]. It is a carminative naturally occurring. Numerous
minerals and nutrients including manganese, iron, magnesium,
calcium, folate, potassium, and copper are present in
peppermint oil. It also contains omega-3 fatty acids, Vitamin A
ISSN
0976-9595
Review Article
Kingdom
Plantae
(unranked)
Angiosperms
(unranked)
Eudicots
(unranked)
Asterids
Order
Lamiales
Species
Menthe
Binomial name
Mentha piperita
Vinutha Moses et al, J Adv Sci Res, 2014, 5(4): 01-03 2
Journal of Advanced Scientific Research, 2014, 5(4)
and Vitamin C [13]. The chemical constituents of peppermint
are rich in menthol, menthone and menthyl esters (menthyl
acetate) [14]. Dried peppermint typically has 0.3-0.4% of
volatile oil containing menthol (7-48%), menthone (20-46%),
menthyl acetate (3-10%), menthofuran (1-17%) and 1, 8-cineol
(3-6%). It also contains small limonene, pulegone,
caryophyllene and pinenel15 as shown in Fig 2.
Fig. 1: Structures of components of mint oil [11]
Sustrikova and Salamon [16] in 2004, claimed oil is
contained in little vesicles existing throughout the plant, and
visible in the leaves. According to Jorge Gutierrez et al [17]
essential oils are oily liquids that are fragrant and volatile. They
are found in the stem, bark and leaves of the plant, formed by
some specialized group of cells. The oil is commonly stored in
bags like glands on the lower sides of the leaves.
Arun K. Tripathi et al [18] declared that terpenes, benzene
derivatives, hydrocarbons and other miscellaneous compounds
are the four main groups of volatile components present in
essential oil. They are the complex secondary metabolites,
having strong odour. The nature of oil is lipophilic.
According to Shrivastava Alankar [19] peppermint oil have
its own characteristic odour and taste, it appears to be yellow,
pale greenish-yellow in colour or may be colourless. The oil is
soluble in 70% ethanol and slightly soluble in water.
Salient properties of mint oil are as shown in Table 2.
Table 2. Properties of Mint Oil [12]
2. APPLICATIONS OF MINT OIL
Gokalp Iscan et al [20] suggested that apart from using the
mint oil in food and herbal tea preparation, it can also be used
for medicinal therapy that includes carminative, anti-
inflammatory, antispasmodic, antiemetic, diaphoretic, analgesic
and stimulant application. It can be further used in the
treatment of nausea, anorexia, ulcer, bronchitis, sinus,
toothache, itching and skin irritation, cold and flu, headache
muscle pain, infections caused by bacteria and virus. Release
stress, mental exhaustion and depression, and it helps in
strengthening the immune system. It also acts as mosquito
repellent.
In 2002 Patra et al [21], showed that Mentha spicata oil is
used as a nitrification inhibitor for maximum yield of
Japanese mint. The sensory test and effects of the fragrance of
peppermint oil on human inhalation was potentially carried out
by many researches and many of them declared harmless. Sara
Burt [22] in 2004 carried out antibacterial activity of essential
oils on some bacteria and exhibited that gram positive bacteria
is more susceptible than gram negative bacteria. Grigoleit et al
[23] stated in 2004, the choleretic and antifoaming effects of
peppermint oil showed an added advantage to medicinal
application.
Benjamin Kligler and Sapna Chaudhary [14] in 2007
exhibited the potential effect of mint oil in reducing spasm,
irritable bowel syndrome (IBS) and non-ulcer dyspepsia. This
was already mentioned by Pittler and Ernst [24] in 1998,in
addition,it can be used as an excellent pain killer. Gutierrez et
al [25] in 2008 observed that peppermint oil showed excellent
antimicrobial properties; hence it acts against the bacteria that
affect food and food-borne pathogens. Alok et al [26] in 2012
demonstrated the potential benefit of increased productivity
and better soil condition by recycling of menthol mint via
vermicomposting. Abdelrazzaq et al [27] in 2013 showed that
antiradical values were significantly higher for leaf under soil
cultivation than leaf under soiless cultivation.
In a comparative study, experimented by Mei et al [28] in
2013 between peppermint and chocolate mint, antimicrobial
activity of peppermint was stronger and it also showed better
properties than the other. Neha Sambe et al [29] in 2014
showed that the mint oil and its three lead compounds showed
transitional properties and hence has a significant effect on the
secretion of enzymes during infection by the fungal cell.
According to Punitha et al in 2014 [30] inhibition zone
diameters obtained in well diffusion assays showed the
effectiveness of essential oils by forming methicillin-resistant
against biofilms.It also showed nearly equal antimicrobial effects
on both gram-positive and gram-negative bacteria.
3. CONCLUSION
Mint oils are one of the most popular and widely used
essential oils because of its components such as menthol and
isomenthonone [31] and the effective properties of these
components contribute a lot to medicinal application, further
development and advanced research is carried to enhance its
properties and increase its applications making peppermint a
valuable and precious plant.
4. REFERENCES
1. Pramila DM, Xavier R, Marimuthu K, Kathiresan S, Khoo ML,
Senthilkumar M, Sathya K & Sreeramanan S, Journal of Medicinal
Plants Research, 2012; 2:331-335.
S. N.
Properties
Values
1
Molecular weight
965.51672 g/mol
2
Density
0.896-0.908 g/cm3 (25 deg)
3
Molecular Formula
C62H108O7
4
Boiling point
82-93°C.
5
Solubility
Slightly soluble in water and
alcohol
6
Specific Gravity
0.90 g/mL at 20ºC.
7
Refractive Index
1.421
Vinutha Moses et al, J Adv Sci Res, 2014, 5(4): 01-03 3
Journal of Advanced Scientific Research, 2014, 5(4)
2. Linnaeus C, Liddell, Henry George; Scott, Robert; A Greek–
English Lexicon at the Perseus Project on Species Plantarum,
1753; 2:576-577.
3. Aflatuni, Abbas, Uusitalo J, Ek S, Hohtola A. Journal of Essential
Oil Research, 2005; 17:66-70.
4. Markus Lange B and Rodney Croteau. Current Opinion in Plant
Biology, 1999; 2:139-144.
5. Brickell, Christopher; Cole, Trevor:The American Horticultural
Society, Encyclopedia of Plants & Flowers. New York, NY, USA:
DK Publishing.2002; 605.
6. Bradley, Fern , Rodale's, All-new Encyclopedia of Organic
Gardening, Emmaus, Pennsylvania, USA: Rodale
Press,1992; 390.
7. Rayment W J,In Depth Info on Pepper-Mint, 1999; Website:
www.InDepthInfo.com.
8. www.herbs2000.com
9. PDR for Herbal Medicines: Thomson Healthcare, 4th Edition:
640.
10. Eccles R. J. Pharm. Pharmacol, 1994; 46 (8):618-630.
11. Brown B, Hart JM, Wescott MP, Christensen NW. Better Crops,
2003; 87:4.
12. http://www.druginfosys.com.
13. www.wikipedia.com.
14. Benjamin Kligler and Sapna Chaudhary. American Academy of Family
Physicians, 2007; 75:1027-30.
15. Coleman WM, Lawrence BM, Cole SK. Journal of Chromatographic
Science, 2002; 40:214-216.
16. Sustrikova A, Salamon I. Hort. Sci. Prague, 2004; 31:31-36.
17. Jorge Gutierrez, Catherine Barry-Ryan, Paula Bourke.
International Journal of Food Microbiology, 2008; 124:91-97.
18. Arun K Tripathi, Shikha Upadhyay, Mantu Bhuiyan, Bhattacharya
PR. Journal of Pharmacognosy and Phytotherapy, 2009; 1:5.
19. Shrivastava Alankar. Asian Journal of Pharmaceutical and Clinical
Research, 2009; 2:27-33.
20. Gokalp Iscan, Nese Kirimer, Mine Kurkcuoglu, K. Husnu Can
Baser and Fatih Demirci. Journal of agricultural and food chemistry,
2002; 50:3943-3946.
21. Usha Kiran and Patra DD. Biosource Technology, 2002; 3:267-276.
22. Sara Burt. International Journal of Food Microbiology, 2004; 94:223-
253.
23. Gregoleit HG, Gregoleit P. Phytomedicine, 2004; 12:612-616.
24. Pittler MH and Ernst E. The American Journal of Gastroenterology,
1998; 93:1131-1135.
25. Jorge Gutierrez, Catherine Barry-Ryan and Paula Bourke.
International Journal of Food Microbiology, 2008; 124:91-97.
26. Kalra A, Shukla Saket, Singh Rakshapal, Verma RK, Chandra M,
Singh S et al., Renewable Agriculture and Food Systems, 2012;1-8.
27. Abdelrazzaq Al-Tawaha, Ghazi Al-Karaki, Adnan Massadeh.
Advances in Environmental Biology, 2013; 7:902-910.
28. Mei-Lin Tsai, Chin-Tung Wu, Tsen-Fang Lin, Wei-Chao Lin, Yu-
Chun Huangand Chao-Hsun Yang. Tropical Journal of Pharmaceutical
Research, 2013; 14:577-582.
29. Samber N, Varma Ajit, Manzoor Nikhat. International Journal of
Innovative Research in Science Engineering and Technology, 2014;
3:9404-9411.
30. Thambidurai P, Kannaiyan M, Ponnusamy V, Raja V, Selvam S,
Murugesan B, Chinasamy K. Asian Journal of pharmaceutical and
Cinical Research, 2014; 7:220-225.
31. Prakash N and Yunus M. International Journal of Environment, 2013;
2:16-25.