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Abstract

Ectoparasitism in animals has become an issue of great concern that needs to be resolved to prevent huge economic losses occurring to livestock industry all over the world. Synthetic adrugs have been playing a major role in controlling ectoparasites, but their frequent and irrational use has resulted in drug resistance to routinely used chemicals and their residual effects on food and environment. Therefore, this approach of using chemical acaricides and insecticides is losing its popularity and effectiveness in controll ing ectoparasites. So, the development of alternative approaches in ectoparasite management is currently required. Among alternative protocols, plants and their essential oils have played remarkable role in controlling different ectoparasites (ticks, flies, mites, lice) of veterinary importance. Essential oils have been proved to be cheaper, more effective and safer therapeautic agents against different ectoparasites of livestock importance.
BOLETÍN LATINOAMERICANO Y DEL CARIBE
DE PLANTAS MEDICINALES Y AROMÁTICAS
17 (5): 441 - 452 (2018)
© / ISSN 0717 7917 / www.blacpma.usach.cl
Revisión | Review
441
Acaricidal and insecticidal effects of essential oils against
ectoparasites of veterinary importance
[Efectos acaricidas e insecticidas de los aceites esenciales contra los ectoparásitos de importancia veterinaria]
Asghar Abbas1, Rao Zahid Abbas2, Sabiqaa Masood, Zafar Iqbal2, Muhammad Kasib Khan2,
Muhammad Kashif Saleemi3, Muhammad Asif Raza1, Muhammad Shahid Mahmood4,
Junaid Ali Khan5 & Zia ud Din Sindhu2
1Department of Veterinary and Animal Sciences, Muhammad Nawaz Shareef University of Agriculture, Multan, Pakistan
2Department of Parasitology, University of Agriculture, Faisalabad, Pakistan
3Department of Pathology, University of Agriculture, Faisalabad, Pakistan
4 Institute of Microbiology, University of Agriculture, Faisalabad, Pakistan
5Institute of Pharmacy, Physiology and Pharmacology, University of Agriculture, Faisalabad, Pakistan
Contactos | Contacts: Rao Zahid ABBAS - E-mail address: raouaf@hotmail.com
Abstract: Ectoparasitism in animals has become an issue of great concern that needs to be resolved to prevent huge economic losses
occurring to livestock industry all over the world. Synthetic adrugs have been playing a major role in controlling ectoparasites, but their
frequent and irrational use has resulted in drug resistance to routinely used chemicals and their residual effects on food and environment.
Therefore, this approach of using chemical acaricides and insecticides is losing its popularity and effectiveness in controlling ectoparasites.
So, the development of alternative approaches in ectoparasite management is currently required. Among alternative protocols, plants and
their essential oils have played remarkable role in controlling different ectoparasites (ticks, flies, mites, lice) of veterinary importance.
Essential oils have been proved to be cheaper, more effective and safer therapeautic agents against different ectoparasites of livestock
importance.
Keywords: Plants; Essential oils; Ectoparasites; Animals
Resumen: En los animales el ectoparasitismo se ha convertido en un tema de gran preocupación que debe resolverse para evitar que se
produzcan grandes pérdidas económicas para la industria ganadera en todo el mundo. Los aditivos sintéticos han desempeñado un papel
importante en el control de los ectoparásitos, pero su uso frecuente e irracional ha dado como resultado la resistencia a los fármacos
utilizados habitualmente y efectos residuales sobre los alimentos y el medio ambiente. Por lo tanto, el enfoque basado en el uso de acaricidas
e insecticidas químicos está perdiendo popularidad y efectividad en el control de los ectoparásitos. Por lo tanto, actualmente se requiere el
desarrollo de enfoques alternativos en el manejo de ectoparásitos. Entre los protocolos alternativos, las plantas y sus aceites esenciales han
jugado un papel notable en el control de diferentes ectoparásitos (garrapatas, moscas, ácaros, piojos) de importancia veterinaria. Se ha
demostrado que los aceites esenciales son agentes terapéuticos más baratos, más efectivos y más seguros contra diferentes ectoparásitos de
importancia ganadera.
Palabras clave: Plantas; Aceites Esenciales; Ectoparásitos; Animales
Recibido | Received: May 14, 2018
Aceptado | Accepted: August 6, 2018
Aceptado en versión corregida | Accepted in revised form: August 24, 2018
Publicado en línea | Published online: September 30, 2018
Este artículo puede ser citado como / This article must be cited as: A Abbas, RZ Abbas, S Masood, Z Iqbal, MK Khan, MK Saleemi, MA Raza, MS Mahmood, JA Khan, ZD
Sindhu. 2018. Acaricidal and insecticidal effects of essential oils against ectoparasites of veterinary importance. Bol Latinoam Caribe Plant Med Aromat 17 (5): 441 452
Abbas et al. Acaricidal and insecticidal effects of essential oils against ectoparasites of veterinary importance
Titulo corto)
Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas/442
INTRODUCTION
Parasitic diseases account for important health hazard
in man and animal in tropical countries. Ectoparasites
cause serious threat to animalshealth and economy all
over the world. They can cause annoyance, irritation,
skin infection, anaemia, tick fever as well as act as a
vector for various devastating diseases of livestock
importance (Abbas et al., 2014; Yadav et al., 2017).
Among ectoparasites, tickborne infections are
recognized as most devastating because of causing
huge economic losses (Chen et al., 2014; Demessie
and Derso, 2015; Opara et al., 2016). Likewise,
ectoparasites are of great concern due to their
increasing prevalence, zoonotic potential and causing
lowered animal productivity (Jabbar et al., 2015;
Zahid et al., 2016; Zaman et al., 2017a; Zaman et al.,
2017b).
Ectoparasites infecting various species of
animals are controlled by using synthetic insecticides
which is mostly practiced method throughout the
world in spite of several problems like development
of resistance, public concern in terms of residue in
food and environment pollution (Maxwell et al.,
2002; El-Seedi et al., 2017; Showler, 2017).
Therefore, use of insecticides has been limited due to
development of insecticidal drug resistance in ticks
(Olivares-Pérez et al., 2011; Foil et al., 2004; El-
Seedi et al., 2017), lice (Ellse et al., 2012), flies
(Showler, 2017) and mites (Beugnet et al., 1997).
Due to resistance problems alternative
options are being incorporated in strategic and
integrated parasite control programs (Masood et al.,
2013; Abbas et al., 2017a; Abbas et al., 2017b; Idris
et al., 2017; Khan et al., 2017). Among alternatives,
the use of essential oils has been an area of focused
research in several countries (Álvarez et al., 2008;
Khaliq et al., 2015; Liaqat et al., 2016).
Plants extracts and essential oils have been
extensively used in controlling diseases of parasitic,
viral and bacterial origin (Ibrahim et al., 2001; Ntalli
et al., 2010; Ellse et al., 2013; Ellse & Wall, 2014;
Aslam et al., 2016; Awaad et al., 2016; Chen et al.,
2016; Fang et al., 2016; Sands et al., 2016; Esmacily
et al., 2017; Radsetoulalova et al., 2017; Sharifi-Rad
et al., 2017).
Herbal medication has become an appealing
approach and it has gained great importance in
tropical and subtropical regions especially in Asia
and Africa (Habeeb, 2010; Fang et al., 2016; Ijaz et
al., 2016; Rehman et al., 2016; Niroumand et al.,
2016; Showler, 2017; Qureshi et al., 2017). Scientists
and researchers all over the world have proved that
the phytochemicals or essential oils obtained from
different plants have ovicidal, larvicidal, adulticidal
and repellent effects against ectoparasites (Abbas et
al., 2014; Fang et al., 2016). Efficiency of botanical
driven products and essential oils is frequently
ascribed due to their main constituents which have
diverse properties and positive effects (Yang et al.,
2003; Cal, 2006; Birkett et al., 2011; Abbas et al.,
2014; El-Seedi et al., 2017).
This review estimates the potential essential
oils in controlling ectoparasites of veterinary
importance with their possible mechanism of action.
Effects against Ticks
A lot of work has been done in last decade on
investigating the acaricidal response of different
essential oils against ticks of Ixodidae family (hard
ticks). Essential oils of Ageratum houstonianum have
shown remarkable effects against ticks biting
goats.There was a 94.9% decrease in the counting of
biting ticks on goats treated with essential oils of
Ageratum houstonianum (Pamo et al., 2005). In an in
vitro experiment essential oils derived from Thymus
vulgaris, Dorystoechas hasata and Mentha longifolia
were tested through larval immersion test which
resulted in 99% mortality of Rhipicephalus microplus
larvae after exposure to eachoil (0.1% soultion) (Koc
et al., 2013). Essential oils of Pimenta dioica and
Cuminum cyminum were also effective against
Rhipicephalus microplus in 1.26% and 2.49%
solution of each oilwhile essential oil of Ocimum
basilicum had no larvae killing possessions, even at
the quantity of 19.9% (Martinez-Velazquez et al.,
2011). Essential oil of Hypericum polyanthemum
(Ribeiro et al., 2007) and Calceolaria serrata
(Ribeiro et al., 2008) caused no effect on mortality of
two species of ticks including Rhipicephalus
sanguineus and Rhipicephalus microplus. In another
study, the essential oils of Melaleuca alternifolia (Iori
et al., 2005), Satureja thymbra (Cetin et al., 2010)
and Origanum minutiflorum (Cetin et al., 2009) were
proved to be effective against hard ticks.
In a recent in vitro study, essential oils of
Conyza dioscoridis, Artemisia herba-alba and
Calendula officinalis have shown high repulsive
activity against hard ticks (El-Seedi et al., 2017). In
another in vivo study protective action of Tagetes
minuta (Asteraceae) essential oil against
Rhipicephalus microplus was reported and results
suggested that Tagetes minuta was greatly effective
Abbas et al. Acaricidal and insecticidal effects of essential oils against ectoparasites of veterinary importance
Titulo corto)
Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas/443
against ticks (Andreotti et al., 2013). Previously,
essential oil of Tagetes minuta essential oil proved to
be 95% effective for controlling the different tick
species including Rhipicephalus microplus,
Rhipicephalus sanguineus, Amblyomma cajennense
and Argas miniatus. The efficacy of essential oil was
accesssed by adult immersion and larval packet tests
(Garcia et al., 2012).
The acaricidal potential of essential oils may
be accredited due to the action of their volatile
components and constituents (Kim et al., 2007;
George et al., 2009; Cetin et al., 2010).
Effects against Mites
Essential oils are also effective against various mites
infecting animals. In an in vitro study, the essential
oils of plants such as Eugenia caryophyllata,
Coriandrum sativum and Juniperus oxycedrus were
proved to be effective against Dermanyssus gallinae
(poultry red mite) and caused 99.9% mortality (Kim
et al., 2004). In a recent study, essential oils derived
from plants such as clove, palmarosa, tea tree, and
eucalyptus species have shown potential against
Sarcoptes scabiei (Itch mite). Results of study
demonstrated that essential oils of these plants are
potential alternative products to treat Sarcoptes
scabiei infections in animals and humans (Fang et al.,
2016). In another trial in which mites were
permittedinteraction with essential oil of
Leptospermum scoparium in closed and open
chambers which showed good results by causing
29.9% mortality rate in open chambers while 80%
mortality rate in closed chambers (George et al.,
2009). Likewise, higher mortality was observed after
treatment of essential oil of Thymus vulgaris in
closed chambersas compared to open chambers
(George et al., 2009). It has been shown that volatile
characteristics in Thymus vulgaris may be enough to
resist Dermanyssus gallinae for up to 10-15 days
(George et al., 2009). Essential oil of Lavandula
angustifolia caused 70% mortality of mites in an in
vitro assay (George et al., 2008).
Some other in vitro studies have
demonstrated that essential oil of Lavandula
angustifolia and most of its ingredients have shown
potential against Psoroptes cuniculi (Perrucci et al.,
1996). Furthermore, essential oil of Cinnamomum
verum (cinnamon) leaf have been revealed to have
great acaricidal effectiveness against Psoroptes
cuniculi on rabbits (Fichi et al., 2007). In an in vitro
trial among four tested commercially available
monoterpenes (SigmaAldrich, Milan, Italy) geraniol
caused 100% mortality of Otodectes cynotis (dog ear
mite) whereas limonene, p-cymene and α-pinene
were proved to be less effective (Traina et al., 2005).
Different experiments have shown that
acaricidal and insecticidal efficacy of essential oils
varies due to difference in composition of essential
oil and concenteration of its active components that
varies in different varieties of same plant and also
part of plant (leaves, roots) (George et al., 2010).
For example, essential oils obtained from different
varieties of Lavandula angustifolia showed marked
differences in toxicity against D. gallinae (George et
al., 2010). Inconsistency in oil composition or
fractions in different varieties and parts of plants is an
inherent problem. Such differences are important
because the precise composition of an essential oil
may determine its acaricidal efficacy (Na et al.,
2011). Furthermore, chemical composition of
essential oils can also vary according to various
factors such as season (for example before or after
flowering), soil conditions including its type and
water availability (Andrade et al., 2011). Another
important factor that effects the chemical
composition of essential oils is genetic composition
of the plant which is in accordance with plant variety.
All such factors including genetic and epigenetic
factors affect the biochemical synthesis of essential
oils in a particular plant. So, the same species of plant
with different chemical composition of essential oil
may produce different biological and therapeutic
effects (Sangwan et al., 2001).
Effects against Flies and Fleas
Essential oils have been also effective against various
flies and fleas infecting different species of animals.
In in vitro test essential oil of Mentha piperita
(peppermint) was found to be effective against larvae
of the Musca domestica (house fly) (Morey and
Khandagle, 2012). In another study nuisance flies
such as Stomoxys calcitrans, Hippobosca equina and
Musca domestica were declined and repelled on
cattle cured with essential oils of Mentha piperita,
Matricaria chamomilla and Cinnamomum camphora
(Khater et al., 2009). Repulsive properties of
essential oil of Myrica gale were evaluated against
Culicoides impunctatus (biting midge) and results
showed that essential oil of Myrica gale have
repulsive effects against Culicoides impunctatus
Abbas et al. Acaricidal and insecticidal effects of essential oils against ectoparasites of veterinary importance
Titulo corto)
Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas/444
(Stuart & Stuart, 1998). Essential oil of Nepeta
cataria also showed good response against Stomoxys
calcitrans in an in vitro study (Zhu et al., 2012).
In another study essential oils including basil,
cinnamon, citronella and thymus essential oils
showed larvicidal activity against Anopheles dirus
and Aedes aegypti mosquito (Pitasawat et al., 2007).
Various experiments have shown that
essential oils play important role in controllingflies
being responsible for myiasis on animals such as
Lucilia cuprina (Callander & James, 2012) and
Synthesiomyia nudiseta (Khalaf et al., 2009). In an in
vitro study conducted on essential oil of Melaleuca
alternifolia (tea tree), major repulsive properties to
larva of Lucilia cuprina have been recorded
(Callander & James, 2012). Essential oils are also
effective against various fleas infecting animals. It
has been observed that essential oil of the Citrus
sinensis (citrus) oil is harmful to Ctenocephalides
felis (cat flea) (Collart & Hink, 1986). Furthermore,
essential oils containing carvacrol and its derivatives
caused death of flea in an in vitro study (Panella et
al., 2005).
In an experiment, insecticidal activity of
essential oils from Origanum onites, Satureja
thymbra and Myrtus communis was evaluated against
different insects. Among all tested the essential oils
of Origanum onites and Satureja thymbra were
effective causing 100% mortality of insects (Ayvaz et
al., 2010).
Effects against Lice
Essential oils are also effective against various types
of lice infecting animals. Various in vitro and in vivo
trials have shown remarkable results against lice of
veterinary importance. In an in vitro study,
effectiveness of essential oil of Melaleuca alternifolia
(tea tree) was evaluated against Bovicolao cellatus
(chewing lice). The essential oils showed their
antilice activity in terms of high mortality (Talbert &
Wall, 2012). Different in vitro assays have displayed
that usage of 1% quantity of tea tree caused 100%
mortality of Bovicola ovis (sheep lice) (Callander
and James, 2012). In another in vitro study, essential
oil of Cinnamomum camphora (camphor) proved to
be the lethal to Haematopinus tuberculatus and
caused ovicidal action on its eggs (Khater et al.,
2009). Essential oil of Lippia multiflora proved to
have excellent potential against body lice, head lice
and scabies mites, with overall efficacy exceeding as
compared to synthetic drugs tested (Oladimeji et al.,
2000).
Mechanism of action of Essential Oils
Acaricidal and insecticidal effects of essential oils are
largely associated with the presence of bioactive
constituents (Boldbaatar et al., 2014). Many botanical
oils and their extracts are composed of more than one
bioactive compound that can exert different modes of
action against ectoparasites (Showler, 2017). Many
studies have revealed that constituents of essential
oils have harmful effect on nervous system of
ectoparasites. For example, terpinen-4-ol, high in
concentrations in tea tree oil, inhibits release of
acetylcholinesterase which is essential for insects for
their activity and synaptic transmission (Bakkalai et
al., 2008; Lopez & Pascual-Villalobos, 2010).
Different compounds of essential oils are also known
to act on Octopamine (circulating-neuromodulator)
and its disruption results in complete breakdown of
nervous system in insects (Hollingworth et al., 1984).
Furthermore, essential oils are hydrophobic in nature
and cause water stress in insects by blocking the
spiracles resulting in suffocation and distressing the
cuticular waxes (Burgess, 2009).
Different studies have shown that essential
oils components act synergistically. This may occur
because some oil components aid cellular
accumulation and absorption of other toxic
components (Yang et al., 2003; Cal, 2006). Although
several hypotheses for this have been proposed, the
underlying mechanism has not been fully elucidated
so far. Synergistic activity observed has long been
speculated to be obtained via complex effects in
several targets due to multiple modes-of-action by
different components (Tak & Isman, 2017).
However, despite this complexity in their modes-of-
action, the synergistic or antagonistic effects in
essential oil-based insecticides seem to depend upon
concentration of major constituents of particular
essential oil (Tong & Coats, 2012).
Mostly essential oils are rapidly absorbed
after dermal or oral administration and cross the
blood-brain barrier and interact with receptors in the
central nervous system. Components of essential oils
are fat soluble and have the ability to permeate the
membranes of the skin and act on targets organs
(Adorjan & Buchbauer, 2010). Most essential oil
components are metabolized and either eliminated by
the kidneys in the form of polar compounds (Kohlert
et al., 2000). The same happens with thymol,
Abbas et al. Acaricidal and insecticidal effects of essential oils against ectoparasites of veterinary importance
Titulo corto)
Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas/445
carvacrol, limonene and eugenol. After their oral
administration, sulphate and glucuronide forms have
been detected in urine and in plasma respectively
(Michiels et al., 2008). Due to their volatility nature
and fast metabolism of its active compounds there is
a minimum risk of accumulation in body tissues
(Kohlert et al., 2002).
Essential oils are highly complex mixtures of
volatile compounds (Shibamoto, 2010), including
hydrocarbons (e.g. limonene, pinene), acids (e.g.
benzoic acid, geranic acid), alcohols (e.g. santalol,
linalol), aldehydes (e.g. citral, cuminal), ketones (e.g.
camphor), lactones (e.g. bergaptene), phenols (e.g.
eugenol), phenolic ethers (e.g. anethole), oxides (e.g.
1,8 cineole) and esters (e.g. geranylacetate) (Andrade
et al., 2011).
Limitations of using essential oils
No doubt essential oil have wide potential uses but,
their use remain limited due to toxic effects and
other undesirable effects (Yang et al., 2005).
Essential oils also deteriorate the cell membrane and
cell wall structure cytoplasmic membranes and
organelles of cell including mitochondria and
peroxisomes (Bakkali et al., 2008). Essential oils
disturb the depolarization of mitochondrial
membrane in cell by altering ion channels and effect
ATP synthesis (Vercesi et al., 1997). Essential oils
such as thymol and carvacol have been proved to be
to be lethal for the intestinal cells of mucosa layer
due to lipophilic and hydrophobic nature (Giannenas
et al., 2003). Furthermore, essential oils separated
from Chinese as well Egyptian plants have been
reported to cause fumigant toxicity (Fu et al., 2013).
It should also be taken into account that essential oils
and their components could cause allergic reactions
and symptoms (De Groot & Schmidt, 2016).
Table No. 1
Some important essential oils reported for acaricidal and insecticidal activities
Common Name
Ectoparasite
Reference
Goat weed
Ticks
Kumar et al., 2016
Garlic
Mites
George et al., 2010
wormwood
Ticks
Jaenson et al., 2005
Asso
Ticks
El-Seedi et al., 2017
Neem
Ticks
Nawaz et al., 2015
Mustard
Mites
Kim et al., 2004
Marigold
Ticks
El-Seedi et al., 2017
Purple cleome
Ticks
Ndungu et al., 1999
Clove
Mites
Kim et al., 2004
Stinkweed
Ticks
Lwande et al., 1999
Bay
Mites
Macchioni et al., 2006
Zapania Lam
Ticks
Cruz et al., 2013
Tea tree
Mites
Magi et al., 2006
Sweet Basil
Ticks
Veeramaniet al., 2014
Oregano
Ticks
Kocet al., 2013
Allspice
Ticks
Martinez-Velazquez et al., 2011
Wild marigold
Ticks
Andreottiet al., 2013
Thyme
Mites
George et al., 2009
Abbas et al. Acaricidal and insecticidal effects of essential oils against ectoparasites of veterinary importance
Titulo corto)
Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas/446
Table No. 2
Major constituents of some important essential oils
Scientific Name
Essential Oil
Major Constituent
Reference
Thymus vulgaris L
Thyme
Carvacrol
Thymol
Borneol
Fadliet al., 2011
Thymus pulegioides L
Thyme
Geraniol
Miladinovic et al., 2014
Origanum vulgare L
Oregano
Cymenol
Rosatoet al., 2010
Origanumm ajorana L
Marjoram
4-Terpineol
El-Hosseiny et al., 2014
Salvia officinalis L
Sage
1,8-Cineole
El-Hosseiny et al., 2014
Satureja montana L
Savory
Geraniol
Miladinovic et al., 2014
Ocimum basilicum L
Basil
Linalool
Silva et al., 2015
Aniba rosaeodora Ducke
Rosewood
Linalool
Rosato et al., 2010
Melaleuca Alternifolia Maiden &
Betche ex Cheel
Tea tree
Terpinen-4-ol
Rosato et al., 2010
Pelargonium graveolens L’Her
Geranium
Citronellol
Rosato et al., 2010
Zanthoxylum articulatum Engler
Limão-bravo
Viridiflorol
Spathulenol
Elemol
Rodrigues et al., 2010
Allium sativum L
Garlic
Diallylle disulfide
Thomson & Ali, 2003
Mentha piperita L
Peppermint
Menthol & menthone
Sala, 2011
Azadirachta indica A. Juss
Neem
Hexadecanoic acid
Oleic acid
octadecanoic acid
4-octylphenol
Kurose &Yatagai, 2005
Concluding Remarks
On the basis of previous and recent research on
essential oils against ectoparasites, it is proved that
essential oils are effective in controlling ectoparasites
of livestock importance. The essential oils should be
considered as alternative to chemical insecticides thus
delaying or averting resistance. Essential oils can act
as best alternative in the treatment of ectoparasite
infections. However, most of the studies reported in
this review article are not so well designed and
comprehensive and based on just in vitro trials in
laboratory conditions, therefore, further extensive in
vivotrials and experiments are needed for formulation
and standardization of herbal product from these
essential oilsto be used in field practices.
REFERENCES
Abbas A, Abbas RZ, Khan, JA, Iqbal Z, Bhatti
MMH, Sindhu ZuD, Zia MA. 2014.
Integrated strategies for the control and
prevention of dengue vectors with particular
reference to Aedes aegypti. Pak Vet J 34: 1 -
10.
Abbas A, Iqbal Z, Abbas RZ, Khan MK, Khan JA.
2017a. Immunomodulatory activity of Pinus
radiata extract against coccidiosis in broiler
chicken. Pak Vet J 37: 145 - 149.
Abbas A, Iqbal Z, Abbas RZ, Khan MK, Khan JA,
Sindhu ZD, Mahmood MS, Saleemi MK,
2017b. In vivo anticoccidial effects of Beta
vulgaris (sugar beet) in broiler chickens.
Microb Path 111: 139 - 144.
Adorjan B, Buchbauer G. 2010. Biological properties
of essential oils: an updated review. Flavour
Fragr J 25: 407 - 426.
Álvarez V, Loaiza J, Bonilla R. 2008. In vitro control
of ticks (Boophilus microplus; Acari:
Ixodidae) by plant extracts. Rev Biol Trop
Abbas et al. Acaricidal and insecticidal effects of essential oils against ectoparasites of veterinary importance
Titulo corto)
Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas/447
56: 291 - 302.
Andrade EHA, Alves CN, Guimarães EF, Carreira
LMM, Maia JGS. 2011. Variability in
essential oil composition of Piper dilatatum
L.C. Rich. Biochem Syst Ecol 39: 669 - 675.
Andreotti R, Garcia MV, Cunha RC, Barros JC.
2013. Protective action of Tagetes minuta
(Asteraceae) essential oilin the control of
Rhipicephalus microplus (Canestrini, 1887)
(Acari: Ixodidae) in a cattle pen trial. Vet
Parasitol 197: 341 - 345.
Aslam A, Shahzad MI, Parveen S, Ashraf H, Naz N,
Zehra SS, Kamran Z, Qayyum A, Mukhtar
M. 2016. Evaluation of antiviral potential of
different Cholistani plants against infectious
bursal disease and infectious bronchitis virus.
Pak Vet J 36: 302 - 306.
Awaad MHH, Afify MAA, Zoulfekar SA,
Mohammed FF, Elmenawy MA, Hafez HM.
2016. Modulating effect of peppermint and
eucalyptus essential oils on vVND infected
chickens. Pak Vet J 36: 350 - 355.
Ayvaz A, Sagdic O, Karaborklu S, Ozturk I. 2010.
Insecticidal activity of the essential oils from
different plants against three stored-product
insects. J Insect Sci 10: 1 - 13.
Bakkali F, Averbeck S, Averbeck D, Idaomar M.
2008. Biological effects of essential oilsa
review. Food ChemToxicol 46: 446 - 475.
Beugnet F, Chauve C, Gauthey M, Beert L. 1997.
Resistance of the red poultry mite to
pyrethroids in France. Vet Rec 140: 577 -
579.
Birkett MA, Hassanali A, Hoglund S, Pettersson J,
Pickett JA, 2011. Repellent activity of
catmint, Nepetacataria, and iridoidnepeta-
lactone isomers against Afro-tropical
mosquitoes, ixodid ticks and red poultry
mites. Phytochemistry 72: 109 - 114.
Boldbaatar D, El-Seedi HR, Findakly M, Jabri S,
Javzan B, Choidash B, Goransson U,
Hellman B. 2014. Antigenotoxic and
antioxidant effects of the Mongolian
medicinal plant Leptopyrum fumarioides (L):
an in vitro study. J Ethnopharmacol 155:
599 - 606.
Burgess IF. 2009 The mode of action of dimeticone
4% lotion against head lice, Pediculuscapitis.
BMC Pharmacol 9: 1 - 8.
Cal K. 2006. Skin penetration of terpenes from
essential oils and topical vehicles. Planta
Med 72: 311 - 316.
Callander JT, James PJ. 2012. Insecticidal and
repellent effects of tea tree (Melaleuca
alternifolia) oil against Lucilia cuprina. Vet
Parasitol 184: 271 - 278.
Cetin H, Cilek JE, Oz E, Aydin L, Deveci O,
Yanikoglu A. 2010. Acaricidal activity of
Satureja thymbra L. essential oil and its
major components, carvacrol and gamma-
terpinene against adult Hyalomma
marginatum (Acari: Ixodidae). Vet Parasitol
170: 287 - 290.
Cetin H, Cilek JE, Aydin L, Yanikoglu A. 2009.
Acaricidal effects of the essential oil of
Origanum minutiflorum (Lamiaceae) against
Rhipicephalus turanicus (Acari: Ixodidae).
Vet Parasitol 160: 359 - 361.
Chen Z, Liu, Q, Liu, JQ, Xu, BL, Lv, S, Xia S, Zhou,
XN. 2014. Tick-borne pathogens and
associated co-infections in ticks collected
from domestic animals in central China.
Parasit Vectors 7: 1 - 8.
Chen L, Jiang T, Li X, Wang Q, Wang Y, Li Y,
2016. Immunomodulatory activity of β-
glucan and mannan-oligosaccharides from
Saccharomyces cerevisiae on broiler
chickens challenged with feed-borne
Aspergillus fumigatus. Pak Vet J 36: 297 -
301.
Collart MG, Hink WF. 1986 Sublethal effects of D-
limonene on the cat flea, Ctenocephalides
felis. Entomol Exp Appl 42: 225 - 229.
Cruz EM, Costa-Junior LM, Pinto JA, SantosDA,
Araujo, SA, Arrigoni-Blank MF, Bacci L,
Alves PB, Cavalcanti SC, Blank AF, 2013.
Acaricidal activity of Lippia gracilis
essential oil and its major constituents on the
tick Rhipicephalus (Boophilus) microplus.
Vet Parasitol 195: 198 - 202.
De Groot AC, Schmidt E. 2016. Essential oils:
contact allergy and chemical composition;
CRC Press: Boca Raton, FL, USA.
Demessie Y, Derso S. 2015. Tick borne
hemoparasitic diseases of ruminants: A
Review. Adv Biol Res 9: 210 - 224.
El-Hosseiny L, El-Shenawy M, Haroun M, Abdullah
F. 2014. Comparative evaluation of the
inhibition effect of some essential oils with
antibiotics against Pseudomonas aeruginosa.
Int J Antibiot 2014: 1 - 15.
Ellse L, Burden F, Wall R. 2013. Control of the
Abbas et al. Acaricidal and insecticidal effects of essential oils against ectoparasites of veterinary importance
Titulo corto)
Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas/448
chewing louse Bovicola (Werneckiella)
ocellatus in donkeys, using essential oils.
Med Vet Entomol 27: 408 - 413.
Ellse L, Burden F, Wall R. 2012. Pyrethroid tolerance
in the chewing louse Bovicola (Werneckiella)
ocellatus. Vet Parasitol 188: 134 - 139.
Ellse L, Wall R. 2014. The use of essential oils in
veterinary ectoparasite control: a review.
Med Vet Entomol 28: 233-243.
El-Seedi HR, Azeem M, Khalil NS, Sakr HH,
Shaden A, Khalifa M, Awang K, Saeed A,
Mohamed A. Farag MA, Al Ajmi MF,
Palsson K, Borg-Karlson A. 2017. Essential
oils of aromatic Egyptian plants repel
nymphs of the tick Ixodes ricinus (Acari:
Ixodidae). Exp Appl Acarol 73: 139 - 157.
Esmacily M, Bandani A, Zibaee I, Sharijian J, Zare S.
2017. Sublethal effects of Artemisia annua L
and Rosmatinus officinalis L, essential oils
on life table parameters of Tetranychus
urticae (Acari: tetranychidae). Persian J
Acarology 6:
Fadli M, Chevalier J, Saad A, Mezrioui NE,
Hassani L, Pagès JM. 2011. Essential oils
from Moroccan plants as potential
chemosensitisers restoring antibiotic activity
in resistant Gram-negative bacteria. Int J
Antimicrob Agents 38: 325 - 330.
Fang F, Candy K, Melloul E, Bernigaud C, Chai L,
Darmon C, Durand R, Botterel F, Chosidow
O, Izri A, Huang W, Guillot J. 2016. In vitro
activity of ten essential oils against Sarcoptes
scabiei. Parasit Vectors 9: 1 - 8.
Fichi G, Flamini G, Zaralli LJ, Perrucci S. 2007.
Efficacy of an essential oil of Cinnamomum
zeylanicum against Psoroptes cuniculi.
Phytomedicine 14: 227 - 231.
Foil LD, Coleman P, Fragoso-Sanchez HE, Garcia-
Vazquez Z, Guerrero FD, Jonsson NN,
Langstaff IG, Li AY, Machila N, Miller RJ,
Morton J, Pruett JH, Torr S. 2004. Factors
that influence the prevalence of acaricide
resistance and tick-borne diseases. Vet
Parasitol 125: 163 - 181.
Fu C, Wan T, Jiang Z, Wu H, Feng J, Ma Z, Zhang
X. 2013. Fumigation activity of esssential
oils against Culex pipens pallens (Diptera:
Culicidae). Acta Entomol Sin 56: 779 - 785.
Garcia MV, Matias J, Barros JC, Lima DP, Lopes
RS, Andreotti R. 2012. Chemical
identification of Tagetes minuta Linnaeus
(Asteraceae) essential oil and its acaricidal
effect on ticks. Rev Bras Parasitol Vet 21:
405 - 411.
George DR, Callaghan K, Guy JH, Sparagano OAE.
2008. Lack of prolonged activity of lavender
essential oils as acaricides against the poultry
red mite (Dermanyssus gallinae) under
laboratory conditions. Res Vet Sci 85: 540 -
542.
George DR, Sparagano OAE, Port G, Okello E, Shiel
RS, Guy JH. 2009. Repellence of plant
essential oils to Dermanyssus gallinae and
toxicity to the non-target invertebrate
Tenebrio molitor. Vet Parasitol 162: 129 -
134.
George DR, Sparaganon OAE, Port G, Okello E,
Shiel RS, Guy JH. 2010. Environmental
interactions with the toxicity of plant
essential oils to the poultry red mite
Dermanyssus gallinae. Med Vet Entomol
24: 1 - 8.
Giannenas I, Florou-Paneri P, Papazahariadou M,
Christaki E, Botsoglou NA, Spais AB. 2003.
Effect of dietary supplementation with
oregano essential oil on performance of
broilers after experimental infection with
Eimeria tenella. Arch Tierernahr 57: 99 -
106.
Habeeb SM. 2010. Ethno-veterinary and medical
knowledge of crude plant extracts and its
methods of application (traditional and
modern) for tick control. World Appl Sci J
11: 1047 - 1054.
Hollingworth RM, Johnstone EM, Wright N. 1984.
In: Magee PS, Kohn GK, Menn JJ (eds),
Pesticide synthesis through rational
approaches. ACS Symposium Series No.
255, American Chemical Society,
Washington DC, USA.
Ibrahim MA, Kainulainen P, Aflatuni A, Tiilikkala
K, Holopainen JK. 2001. Insecticidal,
repellent, antimicrobial activity and
phytotoxicity of eseential oils: with special
reference to limonene and its suitability for
control of insect pests. Agric Food Sci
Finland 10: 243 - 259.
Idris M, Abbas RZ, Masood S, Rehman T, Farooq U,
Babar W, Hussain R, Raza A, Riaz U. 2017.
The potential of antioxidant rich essential oils
against avian coccidiosis. World's Poult Sci
J 73: 89 - 104.
Abbas et al. Acaricidal and insecticidal effects of essential oils against ectoparasites of veterinary importance
Titulo corto)
Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas/449
Ijaz A, Javed I, Aslam B, Khan JA, Khaliq T,
Rahman ZU, Khan MZ, Iqbal Z, Naeem MA,
Ashraf MM, 2016. Nephroprotective and
antioxidant effects of Moringa oleifera
(Sohanjna) in paracetamol induced
nephrotoxic albino rabbits. Pak Vet J 36:
292 - 296.
Iori A, Grazioli D, Gentile E, Marano G, Salvatore
G. 2005. Acaricidal properties of the
essential oil of Melaleuca alternifoliaCheel
(tea tree oil) against nymphs of Ixodes
ricinus.Vet Parasitol 129: 173-176.
Jabbar A, Abbas T, Sindhu ZU, Saddiqi HA, Qamar
MF, Gasser RB. 2015. Tick-borne diseases of
bovines in Pakistan: Major scope for future
research and improved control. Parasit
Vectors 8: 1 - 13.
Jaenson TGT, Palsson K, Borg-Karlson AK. 2005.
Evaluation of extracts and oils of tick-
repellent plants from Sweden. Med Vet
Entomol 19: 345 - 352.
James PJ, Callander JT. 2012. Bioactivity of tea tree
oil from Melaleuca alternifolia against sheep
lice (Bovico laovis Schrank) in vitro. Vet
Parasitol 187: 498 - 504.
Khalaf AFA, Hussein KT, Shoukry KK. 2009.
Biocidal activity of two botanical volatile oils
against the larvae of Synthesiomyia nudiseta
(Wulp) (Diptera: Muscidae). Egypt Acad J
Biol Sci 2: 89 - 101.
Khaliq T, Mumtaz F, Rahman ZU, Javed I, Iftikhar
A. 2015. Nephroprotective potential of Rosa
damascena mill flowers, Cichorium intybus
Linn roots and their mixtures on gentamicin-
induced toxicity in albino rabbits. Pak Vet J
35: 43 - 47.
Khan MN, Sajid MS, Rizwan HM, Qudoos A, Abbas
RZ, Riaz M, Khan MK, 2017. Comparative
efficacy of six anthelmintic treatments
against natural infection of fasciola species in
sheep. Pak Vet J 37: 65 - 68.
Khater HF, Ramadan MY, El-Madawy RS. 2009.
Lousicidal, ovicidal and repellent efficacy of
some essential oils against lice and flies
infesting water buffaloes in Egypt. Vet
Parasitol 164: 257 - 266.
Kim SI, Yi JH, Tak J, Ahn YJ. 2004. Acaricidal
activity of plant essential oils against
Dermanyssus gallinae (Acari:
Dermanyssidae). Vet Parasitol 120: 297 -
304.
Kim SI, Na YE, Yi JH, Kim BS, Ahn YJ. 2007.
Contact and fumigant toxicity of oriental
medicinal plant extracts against Dermanyssus
gallinae (Acari: Dermanyssidae). Vet
Parasitol 145: 377 - 382.
Koc S, Oz E, Cinbilgel I, Aydin L, Cetin H. 2013.
Acaricidal activity of Origanum bilgeri P.H.
Davis (Lamiaceae) essential oil and its major
component, carvacrol, against adult
Rhipicephalus turanicus (Acari: Ixodidae).
Vet Parasitol 193: 316 - 319.
Kohlert C, Schindler G, Marz RW, Abel G,
Brinkhaus B, Derendorf H; Grafe EU, Veit
M. 2002. Systemic availability and
pharmacokinetics of thymol in humans. J
Clin Pharmacol 42: 731 - 737.
Kohlert C, van Rensen I, Marz R, Schindler G,
Graefe EU, Veit M. 2000. Bioavailability and
pharmacokinetics of natural volatile terpenes
in animals and humans. Planta Medica 66:
495 - 505.
Kumar, KG, Tayade, AB, Kumar, R, Gupta S,
Sharma AK, Nagar G, Tewari SS, Kumar B,
Rawat AK, Srivastava S, Kumar S, Ghosh S.
2016. Chemo-profiling and bioassay of
phytoextracts from Ageratum conyzoides for
acaricidal properties against Rhipicephalus
(Boophilus) microplus (Acari: Ixodidae)
infesting cattle and buffaloes in India. Ticks
Tick Borne Dis 7: 342 - 349.
Kurose K, Yatagai M. Components of the essential
oils of Azadirachta indica A. Juss,
Azadirachta siamensis Velton, and
Azadirachta excels (Jack) Jacobs and their
comparison. J Wood Sci 2: 185 - 188.
Liaqat I, Pervaiz Q, Bukhsh, SJ, Ahmed SI, Jahan N.
2016. Investigation of bactericidal effects of
medicinal plant extracts on clinical isolates
and monitoring their biofilm forming
potential. Pak Vet J 36: 159 - 164.
Lopez MD, Pascual-Villalobos MJ. 2010. Mode of
inhibition of acetylcholinesterase by
monoterpenoids and implications for pest
control. Ind Crops Prod 31: 284 - 288.
Lwande W, Ndakala AJ, Hassanali A, Moreka L,
Nyandat E, Ndungu M, Amiani H, P.M.
Gitu PM, Malonza MM, Punyua DK. 1999.
Gynandropsis gynandra essential oil and its
constituents as tick (Rhipicephalus
appendiculatus) repellents. Phytochemistry
50: 401 - 405.
Abbas et al. Acaricidal and insecticidal effects of essential oils against ectoparasites of veterinary importance
Titulo corto)
Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas/450
Macchioni F, Perrucci S, Cioni P, Morelli L, Castilho
P, Cecchi F. 2006. Composition and
acaricidal activity of Laurus novocanariensis
and Laurus nobilis essential oils against
Psoroptescuniculi .J Essent Oil Res 18: 111
- 114.
Magi E, Jarvis T, Miller I. 2006. Effects of different
plant products against pig mange mites. Acta
Vet Brno 75: 283 - 287.
Martinez-Velazquez M, Castillo-Herrera GA,
Rosario-Cruz R, Flores-Fernandez JM,
Lopez-Ramirez J, Hernandez-Gutierrez R,
Lugo-Cervantes ED. 2011. Acaricidal effect
and chemical composition of essential oils
extracted from Cuminum cyminum, Pimenta
dioica and Ocimum basilicum against the
cattle tick Rhipicephalus (Boophilus)
microplus (Acari: Ixodidae). Parasitol Res
108: 481 - 487.
Masood S, Abbas RZ, Iqbal Z, Mansoor MK, Sindhu
ZD, Zia MA, Khan JA. 2013. Role of natural
antioxidants for the control of coccidiosis in
poultry. Pak Vet J 33: 401 - 407.
Maxwell CA, Msuya E, Sudi M, Njunwa KJ,
Carneiro IA, Curtis CF. 2002. Effect of
community-wide use of insecticide-treated
nets for 3-4 years on malarial morbidity in
Tanzania. Trop Med Int Health 7: 1003 -
1008.
Michiels J, Missotten J, Dierick N, Fremaut D,
Maene P, De Smet S. 2008. In vitro
degradation and in vivo passage kinetics of
carvacrol, thymol, eugenol and
transcinnnamaldehyde along the
gastrointestinal tract of piglets. J Sci Food
Agric 88: 2371 - 2381.
Miladinovic DL, Ilic BS, Kocic BD, Miladinovic
MD. 2014. An in vitro antibacterial study of
savory essential oil and geraniol in
combination with standard antimicrobials.
Nat Prod Commun 9: 1629 - 1632.
Morey RA, Khandagle, AJ. 2012. Bioefficacy of
essential oils of medicinal plants against
housefly, Muscadomestica L. Parasitol Res
111: 1799 - 1805
Na YE, Kim SI, Bang HS, Kim BS, Ahn YJ. 2011.
Fumigant toxicity of cassia and cinnamon
oils and cinnamaldehyde and structurally
related compounds to Dermanyssus gallinae
(Acari: Dermanyssidae). Vet Parasitol 178:
324 - 329.
Nawaz M, Sajid SM, Zulfiqar A, Muhammad W,
Tanveer A, Abid H, Abrar M, S. Asim S,
Muhammad Z, Imran K. 2015. Anti-Tick
Activity of leaves of Azadirachta indica,
Dalbergia sisso and Morus alba against
Rhipicephalus microplus. Acta Parasitol
Globalis 6: 60 - 64.
Ndungu MW, Chhabra SC, Wande WL. 1999.
Cleome hirta essential oil as livestock tick
(Rhipicephalus appendiculatus) and maize
weevil (Sitophilus zeamais) repellent.
Fitoterapia 70: 514 - 516.
Ntalli NG, Ferrari F, Giannakou I, Menkissoglu-
Spiroudi U. 2010. Phytochemistry and
nematicidal activity of the essential oils from
8 Greek lamiaceae aromatic plants and 13
terpene components. J Agric Food Chem
58: 7856 - 7863.
Niroumand MC, Farzaei MH, Karimpour-Razkenari
EE, Amin G, Khamnavi M, Akbarzadeh T,
Shams-Ardekani MR. 2016. An evidence-
based review on medicinal plants used as
insecticide and insect repellent in traditional
Iranian medicine. Iran Rad Crescent Med
18: e22361.
Oladimeji FA, Orafidiya OO, Ogunniyi TA,
Adewunmi TA. 2000. Pediculocidal and
scabicidal properties of Lippia multiflora
essential oil. J Ethnopharmacol 72: 305 -
311.
Olivares-Pérez J, Rojas-Hernández S, Valencia-
Almazan MT, Gutiérrez-Segura I, Míreles-
Martínez EJ. 2011. Prevalence of resistant
strains of Rhipicephalus microplus to
acaricides in cattle ranch in the tropical
region of Tecpan of galeana, Guerrero
Mexico. Pak Vet J 31: 366 - 368.
Opara MN, Santali A, Mohammed BR, Jegede OC.
2016. Prevalence of haemoparasites of small
ruminants in Lafia Nassarawa State: A
Guinea Savannah Zone of Nigeria. J Vet
Adv 6: 1251 - 1257.
Pamo ET, Tendonkeng F, Kana JR, Khan PV,
Boukila B, Lemoufouet J, Miegoue E, Nanda
AS. 2005. A study of the acaricidal
properties of an essential oil extracted from
the leaves of Ageratum houstonianum. Vet
Parasitol 128: 319 - 323.
Panella NA, Dolan MC, Karchesy JJ, Xiong Y,
Peralta-Cruz J, Khasawneh M, Montenieri
JA, Maupin GO. 2005. Use of novel
Abbas et al. Acaricidal and insecticidal effects of essential oils against ectoparasites of veterinary importance
Titulo corto)
Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas/451
compounds for pest control: insecticidal and
acaricidal activity of essential oil components
from heartwood of Alaska yellow cedar. J
Med Entomol 42: 352 - 358.
Perrucci S, Macchioni G, Cioni PC, Flamini G,
Morelli I, Taccini F. 1996. The activity of
volatile compounds from Lavandula
angustifolia against Psoraptes cuniculi.
Phytotherapy Res 10: 5 - 8.
Pitasawat B, Champakaew D, Choochote W, Jitpakdi
A, Chaithong U, Kanjanapothi D,
Rattanachanpichai E, Tippawangkosol P,
Riyong D, Tuetun B, Chaiyasit D. 2007.
Aromatic plant-derived essential oil: an
alternative larvicide for mosquito control.
Fitoterapia 78: 205 - 210.
Qureshi AS, Rehan S, Enbergs H. 2017. Nigella
sativa seed extract affects granulocyte
phagocytosis and lymphocytes proliferation
in goats. Pak Vet J 37: 411 - 414.
Radsetoulalova I, Hubert J, Lichovnikova M. 2017.
Acaricidal activity of plant essential oils
against poultry red mite (Dermanyssus
gallinae). Mendel Net 24: 260 - 265.
Rehman N, Jahan N, Rahman KU, Khan KM, Zafar
F, 2016. Anti-arrhythmic potential of
Coriandrum sativum seeds in salt induced
arrhythmic rats. Pak Vet J 36: 465 - 471.
Ribeiro VLS, Toigo E, Bordignon SAL, Goncalves
K, von Poser G. 2007. Properties of extracts
from the aerial parts of Hypericum
polyanthemum on the cattle tick Boophilus
microplus. Vet Parasitol 147: 199 - 203.
Ribeiro VLS, Avancini C, Goncalves K, Toigo E,
von Poser G. 2008. Acaricidal activity of
Calea serrata (Asteraceae) on Boophilus
microplus and Rhipicephalus sanguineus.
Vet Parasitol 151: 351 - 354.
Rodrigues FF, Costa JG, Coutinho HD. 2010.
Enhancement of the antibiotic activity of
gentamicin by volatile compounds of
Zanthoxylum articulatum. Indian J Med Res
131: 833 - 835.
Rosato A, Piarulli M, Corbo F, Muraglia M, Carone
A, Vitali ME, Vitali C. 2010. In vitro
synergistic antibacterial action of certain
combinations of gentamicin and essential
oils. Curr Med Chem 17: 3289 - 3295.
Sala H. 2011. Aromatherapy: current and emerging
applications. Altern Complement Ther 17:
26 - 31.
Sands B, Ellse L, Wall R. 2016. Residual and
ovocidal efficacy of essential oil-based
formulations in vitro against the donkey
chewing louse Bovicola ocellatus. Med Vet
Entomol 30: 78 84.
Sangwan NS, Farooqi AHA, Shabih F, Sangwan RS.
2001. Regulation of essential oil production
in plants. J Plant Growth Regul 34: 3 - 21.
Sharifi-Rad J, Sureda A, Tenore GC, Daglia M,
Sharifi-Rad M, Valussi M, Tundis R, Sharifi-
Rad M, Loizzo MR, Ademiluyi AO, Sharifi-
Rad R, Ayatollahi A, Iriti M. 2017.
Biological activities of essential oils: from
plants chemoecology to traditional healing
systems. Molecules 22: 70
Shibamoto K, Mochizuki M, Kusuhara M. 2010.
Aroma therapy in anti-aging medicine. J
Anti Aging Med 7: 55 - 59.
Showler AT. 2017. Botanically based repellent and
insecticidal effects against horn flies and
stable flies (Diptera: Muscidae). J Integr
Pest Manag 8: 1 - 11.
Silva AV, da Sousa PJ, Pessôa LFH, de Freitas FRA,
Coutinho DMH, Alves BNL, Lima OE. 2015.
Ocimum basilicum: Antibacterial activity and
association study with antibiotics against
bacteria of clinical importance. Pharm Biol
54: 863 - 867.
Stuart AE, Stuart CLE. 1998. A microscope slide test
for the evaluation of insect repellents as used
with Culicoides impunctatus. Entomol Exp
Appl 89: 277 - 280.
Tak JH, Isman MB. 2017. Penetration-enhancement
underlies synergy of plant essential oil
terpenoids as insecticides in the cabbage
looper, Trichoplusia ni. Sci Rep 7: 1 - 11.
Talbert R, Wall R. 2012. Toxicity of essential and
non-essential oils against the chewing louse,
Bovicola (Werneckiella) ocellatus. Res Vet
Sci 93: 831 - 835.
Thomson M, Ali M. 2003. Garlic [Allium sativum]: a
review of its potential use as an anti-cancer
agent. Current Cancer Drug Targets 3: 67
- 81.
Tong F, Coats JR. 2012. Quantitative structure-
activity relationships of monoterpenoid
binding activities to the housefly GABA
receptor. Pest Manag Sci 68: 1122 -1129.
Traina O, Cafarchia C, Capelli G, Iacobellis NS,
Otranto D. 2005. In vitro acaricidal activity
of four monoterpenes and solvents against
Abbas et al. Acaricidal and insecticidal effects of essential oils against ectoparasites of veterinary importance
Titulo corto)
Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas/452
Otodectes cynotis (Acari: Psoroptidae). Exp
Applied Acarol 37: 141 - 146.
Veeramani V, Sakthivelkumar S, Tamilarasan K,
Aisha SO, Janarthanan S. 2014. Acaricidal
activity of Ocimum basilicum and Spilanthes
acmella against the ectoparasitic tick,
Rhipicephalus (Boophilus) microplus
(Arachinida: Ixodidae). Trop Biomed 31:
414 - 421.
Vercesi AE, Kowaltowski AJ, Grijalba MT,
Meinicke AR, Castilho RF. 1997. The role of
reactive oxygen species in mitochondrial
permeability transition. Biosci Rep 17: 43 -
52.
Yadav PK, Rafiqi SM, Panigrahi PN, Kumar D,
Kumar R, Kumar S. 2017. Recent trends in
control of ectoparasites: A review. J
Entomol Zool Stud 5: 808 - 813.
Yang P, Ma Y, Zheng S. 2005. Adulticidal activity of
five essential oils against Culex pipiens
quinquefasciatus. J Pest Sci 30: 84 - 89.
Yang YC, Lee SH, Lee WJ, Choi DH, Ahn YJ. 2003.
Ovicidal and adulticidal effects of Eugenia
caryophyllata bud and leaf oil compounds on
Pediculus capitis. J Agric Food Chem 51:
4884 - 4888.
Zahid MU, Hussain MH, Saqib M, Neubauer H,
Abbas G, Khan I, Mansoor MK, Asi MN,
Ahmad T, Muhammad G. 2016. Sero
prevalence of Q fever (Coxiellosis) in small
ruminants of two districts in Punjab,
Pakistan. Vect Borne Zoonotic Dis16: 449 -
454.
Zaman MA, Rehman TU, Abbas RZ, Babar W, Khan
MN, Riaz MT, Hussain R, Ghauri T, Arif M,
2017a. Therapeutic potential of Ivermectin,
doramectin and trichlorophan against
Psoroptes ovis in sheep and cattle of
Cholistan. Pak Vet J 37: 233 - 235.
Zaman MA, Iqbal Z, Sindhu ZUD, Abbas RZ, Qamar
MF, 2017b. An overview of plants with
acaricidal and anthelmintic properties. Int J
Agric Biol 19: 957 - 968.
Zhu JJ, Berkebile DR, Dunlap CA, Zhang A, Boxler
D, Tangtrakulwanich K, Behle RW,
Baxendale F, Brewer G. 2012.
Nepetalactones from essential oil of Nepeta
cataria represent a stable fly feeding and
oviposition repellent. Med Vet Entomol 26:
131 - 138.
... These oils comprise of some components which are biologically active and linked with the acaricidal activity of these oils. These components act in a synergistic manner for manifestation of strong acaricidal action (Abbas et al., 2018). The essential oils exert their effect through different routes like inhalation, ingestion or contact with the body surface of the acarids and damage their nervous system functioning (Khater, 2012;Abbas et al., 2018;Castro et al., 2018). ...
... These components act in a synergistic manner for manifestation of strong acaricidal action (Abbas et al., 2018). The essential oils exert their effect through different routes like inhalation, ingestion or contact with the body surface of the acarids and damage their nervous system functioning (Khater, 2012;Abbas et al., 2018;Castro et al., 2018). For example, the oil of Tagetes minuta has shown its effectiveness in control of Rhipicephalus microplus ticks in cattle. ...
Article
Ticks, particularly the Rhipicephalus which are the most prevalent and invasive affect 80% of the cattle population worldwide. Through transmission of pathogens, tick worry and physical damage to the hides, ticks cause economic loss of billions of dollars each year with 1 billion US dollars loss per annum reported only in Latin-America. These losses can be minimized only by successful management of Rhipicephalus ticks. Various strategies like chemical control, vaccination and biological control are aimed at control of Rhipicephalus ticks. There are some serious limitations associated with them like tick resistance, drug toxicity, antigenic variations etc. In contrast to these issues related with chemical tick control, the botanicals particularly the essential oils obtained from aromatic plants of medicinal importance are eco-friendly and non-toxic to most host. In recent years, essential oils-based control of cattle ticks has gained considerable attraction of scientists all over the world as depicted from this review. A comprehensive effort has been made to critically analyze the role of essential oils in controlling Rhipicephalus ticks with particular emphasis on the mode of action of bioactive compounds both as repellents and acaricides. Furthermore, we have pointed out the most important challenges which need to be addressed for development and commercialization of an essential oil based anti-tick product.
... Ectoparasites are major threat that affect animal welfare and are the vector of many bacterial, viral, rickettsial and protozoal, including zoonotic, diseases (Abbas et al., 2014(Abbas et al., , 2018Khater et al., 2018;Tirosh-Levy et al., 2018). Ticks can affect equine welfare directly through blood loss, skin damage, irritation and discomfort, allergy, tick paralysis, or indirectly through morbidity or mortality caused by infectious organisms transmitted (Duell et al., 2013). ...
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Ticks are major external parasites of horses that affect animal welfare and transmit many infections. Little is known about the epidemiology of ticks in horses in Pakistan. Keeping in view the significance of horses and its importance, a cross-sectional study was designed to investigate species distribution, seasonal dynamics and epidemiology of ticks infesting horses in Pakistan. By convenience sampling, 500 horses in two districts (Sargodha and Lahore) of Punjab were screened for the presence of ticks from January to December 2017. Tick samples were collected from horses and identified to species level. Data of temporospatial, host and husbandry practices-related risk factors were recorded in a separate questionnaire. Ticks representing six species were collected i.e. Hyalomma impeltatum (n=52), H. impressum (n=25), H. excavatum (n=9), H. anatolicum (n=3), H. scupense (n=3) and H. dromedarii (n=3). The sex ratio of collected ticks showed 63 (66.32%) male and 32 (33.68%) female. In both districts, predominant species in horses was H. impeltatum. All infested horses had more than one tick species. The overall proportion of tick infested horses was 7% (35/500), which was high in district Lahore (8.15%) than district Sargodha (5.99%). Summer, ≤3 body condition score, satisfactory nutritional status, same breed rearing system, presence of dogs and absence of birds at farms, and activity were important risk factors associated with high equine tick infestation. This is the first report regarding the presence of H. dromedarii in the horses of Punjab. To Cite This Article: Ali S, Ijaz M, Ghaffar A, Oneeb M, Masud A, Durrani AZ and Rashid MI, 2020. Species distribution and seasonal dynamics of equine tick infestation in two subtropical climate niches in Punjab, Pakistan. Pak Vet J, 40(1): 25-30. http://dx.
... Natural products obtained from plant secondary metabolism have been studied in order to select compounds with potential for the development of new acaricides. These compounds have proved to be an interesting alternative in controlling this ectoparasite, because they have varied mechanisms of action and, generally, little mammalian toxicity (Koul et al., 2008;Pavela and Benelli., 2016;Jankowska et al., 2017;Khater et al., 2018;Abbas et al., 2018;Fayaz et al., 2019). Studies conducted with Acmella oleracea (L.) RK Jansen (Astereacea) (synonym -Spilanthes acmella and Acmella ciliata), commonly known as Jambu, have shown that active compounds found in extracts of this plant present potential for the development of botanical acaricides (Marchesini et al., 2018). ...
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The present study was carried out to evaluate and compare the acaricidal activity of different fractions of Acmella oleracea methanolic extract, containing 0.0% (F1), 24.5% (F2), 48.0% (F3) and 100% (F4) of spilanthol, on unfed larvae and engorged females from the same Rhipicephalus microplus population. To obtain these fractions, the crude extract was subjected to different extraction procedures using increasingly polarized solvents to isolate the spilanthol compound. The Larval Packet Test was used to evaluate acaricidal activity in unfed larvae at concentrations ranging from 0.2 to 25.0 mg/mL, while for engorged females, the Adult Immersion Test was performed at concentrations from 3.1 to 25.0 mg/mL. The F1 fraction showed no activity on unfed larvae, while a control percentage of 44.6% was observed at a concentration of 25.0 mg/mL for engorged females. For unfed larvae, the F2 fraction resulted in 95.7% mortality at a concentration of 1.6 mg/mL, with a control percentage of 92.7% for engorged females at a concentration of 12.5 mg/mL. Fractions F3 and F4 had similar activity against unfed larvae, with mortality >84.0% from the concentration of 0.8 mg/mL. This similarity between the fractions was also observed for engorged females from a concentration of 12.5 mg/mL, expressing a control percentage >94.0%. These results demonstrate that the presence of spilanthol is an important factor for the acaricidal activity of A. oleracea extract. Fraction extracts with 24.5, 48 and 100% of spilanthol have similar acaricidal activity on R. microplus.
... et al., 2008, 2011), alternate approaches are being sought for the control of various infectious diseases including coccidiosis (Hussain et al., 2017;Abbas et al., 2017aAbbas et al., , 2017bAbbas et al., , 2017cAbbas et al., , 2019aAbbas et al., , 2019bMahmood et al., 2018). One of these alternates is the use of essential oils which are helpful in maintaining vital physiological functions (Idris et al., 2017;Abbas et al., 2018;Khater et al., 2018;Ahmad et al., 2019;Fayaz et al., 2019) but consumption of these oils has been reported for causing cell membrane damage in laboratory animals. ...
... Many botanicals and their products have been reported to have shown diverse biological effects in birds and ruminants as proven by different studies (Abbas et al., 2017(Abbas et al., , 2018. In current study in vivo anticoccidial effects of C. sinensis extract were measured in terms of different parameters such as lesion, oocysts scores, oocysts per gram of feces (OPG), feed conversion ratio, mortality rate and such type of anticoccidial parameters have also been evaluated in recent studies (Hong et al., 2016;Gadelhaq et al., 2018). ...
... This may lead to the risk of antibiotic resistance in humans due to the consumption of poultry products, containing antibiotic residues (Kamollerd et al., 2016). Thus, the use of antibiotics and chemicals should be prohibited in Pakistan and novel alternatives should be searched out for controlling infectious diseases (Abbas et al., 2017a, b, c;Abbas et al., 2018;Idris et al., 2017). Probiotics can also serve as an alternative approach to control Salmonella issue in poultry (Amara and Shibal, 2015). ...
... Many botanicals and their products are reported to have excellent effect against various diseases and particularly against coccidiosis as proven by different in vitro and in vivo studies ( Abbas et al., 2015Abbas et al., , 2017aAbbas et al., , 2017bAbbas et al., , 2017cAbbas et al., , 2018Idris et al., 2017;Khater et al., 2018;Mahmood et al., 2018). In present study like that of previous studies (Awais et al., 2014;Hong et al., 2016;Gadelhaq et al., 2018), in vivo anticoccidial effect of Trachyspermum ammi was measured in terms of different parameters such as feed conversion ratio, mortality rate, lesion, fecal and oocyst scores. ...
... Different types of domesticated animals such as buffaloes, cattle, goats, sheep, poultry, horses, donkeys and camel are included in livestock (Khan, 2004). Parasitic diseases are the major issues to the poor farming community in tropical and subtropical areas in particular Pakistan (Jonsson, 2006;Khan et al., 2017;Mehmood et al., 2017;Naqvi et al., 2017;Zaman et al., 2017;Abbas et al., 2018;Khater et al., 2018). Among ectoparasites, ticks are blood-sucking parasites associated with mammals, birds and reptiles (Aslam et al., 2015;Ali et al., 2016) which, also transmit diseases in its host. ...
... Parasitic diseases have been great threat in livestock production systems (Abbas et al., 2017a(Abbas et al., , 2017b(Abbas et al., , 2017c(Abbas et al., , 2018Mehmood et al., 2017;Naqvi et al., 2017;Hanem et al., 2018). Fascioliasis is an economically important disease of sheep and goat, which is caused by Fasciola (F.) hepatica, a flatworm belonging to class Trematoda. ...
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Fascioliasis, caused by Fasciola (F.) hepatica, affects a large number of animals in terms of morbidity, production losses, treatment cost and high mortality rate. Economic losses of about 3.6 billion US$ per year have been reported due to this parasite throughout the world. The present study was planned to investigate the prevalence and related risk factors of F. hepatica in sheep and goat population in District Chakwal, Punjab, Pakistan. To this end, a total of 384 serum samples were collected from sheep and goats in different tehsils of District and examined through enzyme-linked immunosorbent assay (ELISA) by using Excretory/Secretary antigen coated plates. A predesigned questionnaire was also used to collect the data of related risk factors. The overall prevalence of fascioliasis in the small ruminant population was 37.24%. The highest prevalence was observed in tehsil Talla Gang, followed by Chakwal, Kallar Kahar and Choa Saiden Shah. Fascioliasis was more prevalent in females as compared to males. According to age groups, the prevalence was higher in animals with 6-12 months of age. At the species level, sheep had higher prevalence as compared to goats and this prevalence was higher in animals went for grazing than those fed through stalls. Among different breeds of goats, Teddy breed had a higher prevalence of F. hepatica than those of Beetal and mixed breeds of goat.
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House flies are global pests and notoriously difficult to control. Essential oils of vetiver, cinnamon, and lavender and their blends were tested for toxic and repellent effects against larval and adult flies. All of the oils had moderate toxicity for eggs. Mortality of 2nd instar larvae was 57–78% in dipping assays, 38–100% in contact assays, and 94–100% in treated media. Lavender was less effective (38% mortality) than the others (91–100%) in contact bioassays. Oil blends were not more effective against larvae than individual oils. Vetiver and cinnamon oils were strongly repellent (84 and 78%, respectively) for larvae in treated media. None of the oils were repellent for adult house flies in olfactometer assays, but testing of additional products demonstrated significant repellency for neem oil, p‐menthane‐3,8‐diol (PMD), and vanillin. Contact/fumigant toxicity of vetiver, cinnamon, and lavender oils was 100%, significantly higher than mortality from sunflower oil (67%). Blends of oils were not more effective against adults than the individual oils, but blends diluted with sunflower oil were as effective as the individual oils. Essentials oils of vetiver and cinnamon may have potential for fly management in situations where conventional insecticides cannot be used.
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Parasitic diseases account for important health hazard in man and animal in tropical countries like India. Ectoparasites infestation causes a serious loss in health and economy every year in India. They can cause annoyance, irritation, skin infection, anaemia, tick fever as well as act as a vector for various devastating diseases. Thus, ectoparasites control is a matter of great concern. Various chemical acaricides have been prescribed since last 50 years. But, their residual effect, adverse side effect, and resistance are a matter of concern now days. Hence, biological control of ectoparasites gains prime importance in many parasite control program. The present review critically analyzes the different methods for controlling the ectoparasites with special emphasis on the newer approach of the biological, immunological, genetic and pheromone method.
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