ArticlePDF Available

Abstract

For the establishment of the distinctness among 31 accessions of Oryza glaberrima, eight morphological traits following Distinctness, Uniformity and Stability test (DUS) studied. Out of 31 accessions studied, two accessions viz., EC861804, EC861805 were found to be highly distinct as they possessed distinct traits namely purple split ligule, medium green leaves, erect flag leaf, presence of awn along with lodging tolerance indicating their usefulness as donors for crop improvement programmes. Analysis of variance revealed significant differences for all the characters studied. Phenotypic coefficient variation was higher than genotypic coefficient variation and magnitude of PCV and GCV was high for number of productive tillers and spikelets per panicle. High heritability coupled with high genetic advance was observed for days to 50 per cent flowering and number of spikelets per panicle which suggested the presence of high additive gene action which would respond to selection owing their high genetic variability and transmissibility.
Int.J.Curr.Microbiol.App.Sci (2018) 7(12): 1288-1303
1288
Original Research Article https://doi.org/10.20546/ijcmas.2018.712.159
Characterization of African Rice Germplasm for Morphological and Yield
Attributing Traits
V.G. Ishwarya Lakshmi1,4, C. Gireesh1*, M. Sreedhar2, S. Vanisri3, P.S. Basavaraj1,4, B.
Muralidhara1, M.S. Anantha1, G. Padmavathi1, A.R. Fiyaz1, B. Jyothi1, C. Suvarna
Rani1, Bidyasagar Mandal1 and L.V. Subba Rao1
1ICAR-Indian Institute of Rice Research, Rajendranagar, Hyderabad - 500 030, India
2MFPI-Quality control Lab, Department of Genetics and Plant Breeding, College of
Agriculture, Rajendranagar, Hyderabad
3Department of Molecular Biology and Biotechnology, Institute of Biotechnology,
Rajendranagar, Hyderabad
4Department of Genetics and Plant Breeding, College of Agriculture, PJTSAU,
Rajendranagar, Hyderabad
*Corresponding author
A B S T R A C T
Introduction
Rice (Oryza sativa L.) is the principal food
grain crop in India and it is being cultivated in
43.9 million ha with total of production of
109.6 million tonnes (Indiastat, 2016-17).
Since the release of semi dwarf rice varieties
in 1960’s in India and elsewhere, the genetic
gain of yield potentiality among widely
cultivated semi dwarf rice varieties is
stagnated due to narrow genetic base.
Therefore, it is inevitable to broaden the
genetic base of modern rice varieties by
infusing genomic regions from related species
of Oryza. Oryza glaberrima, endemic to
Africa, is reported to have many useful traits
International Journal of Current Microbiology and Applied Sciences
ISSN: 2319-7706 Volume 7 Number 12 (2018)
Journal homepage: http://www.ijcmas.com
For the establishment of the distinctness among 31 accessions of Oryza glaberrima, eight
morphological traits following Distinctness, Uniformity and Stability test (DUS) studied.
Out of 31 accessions studied, two accessions viz., EC861804, EC861805 were found to be
highly distinct as they possessed distinct traits namely purple split ligule, medium green
leaves, erect flag leaf, presence of awn along with lodging tolerance indicating their
usefulness as donors for crop improvement programmes. Analysis of variance revealed
significant differences for all the characters studied. Phenotypic coefficient variation was
higher than genotypic coefficient variation and magnitude of PCV and GCV was high for
number of productive tillers and spikelets per panicle. High heritability coupled with high
genetic advance was observed for days to 50 per cent flowering and number of spikelets
per panicle which suggested the presence of high additive gene action which would
respond to selection owing their high genetic variability and transmissibility.
K e yw or ds
Oryza glaberrima,
DUS, Genetic
variability,
Heritability,
Genetic advance
Accepted:
12 November 2018
Available Online:
10 December 2018
Article Info
Int.J.Curr.Microbiol.App.Sci (2018) 7(12): 1288-1303
1289
such as resistance to biotic, abiotic stresses
(Fofana and Cloutier, 2008) along with early
maturity. In addition, O. glaberrima is also a
potential source of genes to enhance milling,
cooking, eating qualities of indica rice. In
India, O. glaberrima is used in limited extent
for genetic improvement of indica rice as very
less systematic efforts have been undertaken
in India to utilize the wealth of African species
for genetic improvement of indica rice
varieties (Sarla and Swamy, 2005).
Characterization of a variety is useful to
identify and avoid duplication, enabling rice
breeders to exploit a wide range of genotypic
diversities for further crop improvement
practices to increase the rice productivity.
Generally, the morphological traits are
qualitative in nature and are stable over
generations (Raut, 2003), making them more
reliable as morphological markers for the
characterization of varieties. Development of
high yielding cultivars with wide adaptability
is the ultimate aim of plant breeders.
Knowledge of genetic variability present in a
given crop species for the character under
improvement is of paramount importance for
the success of any plant breeding program for
broadening the gene pool of crops (Ahmad et
al., 2011). Heritability provides information
on the extent to which a particular
morphogenetic character can be transmitted to
successive generations and also influences the
choice of selection procedures used by the
plant breeder to decide which selection
methods would be most useful to improve the
character (waqar et al., 2008). Characters with
high heritability can easily be fixed with
simple selection, resulting in quick progress.
However, it has been accentuated that
heritability alone has no practical importance
without genetic advance (Najeeb et al., 2009).
A systematic study analysis of genetic
diversity is essential to exploit the inherent
variability and to broaden the genetic base of
rice cultivars (Rout et al., 2017). Thus, the
present study was conducted to characterize
understand the performance of African rice
germplasm for eight morphological traits
along with a study on genetic variability,
heritability and genetic advance for grain yield
and its component characters. The knowledge
gained in the present study is will be useful for
exploitation of genetic wealth of African rice
for genetic improvement of Indian cultivars.
Materials and Methods
Thirty one O. glaberrima lines received from
IRRI (International Rice Research Institute)
were sown in dry bed during Kharif2017 at the
ICAR-Indian Institute of Rice Research,
Hyderabad. Twenty one days old seedlings of
each accession were transplanted by adopting
a spacing of 20 cm between rows and 15 cm
between plants in a Randomized Block Design
with two replications. Morphological
characterization was carried out using eight
DUS characters. Visual observations were
recorded on single plant basis on five
randomly selected plants in each accession at
appropriate growth stages on eight qualitative
characters viz., Coleoptile colour, leaf
intensity of green colour, culm type, leaf
anthocyanin colouration, leaf ligule, flag leaf
characteristics, panicle awn and stem
anthocyanin colouration. Data was recorded
on 10 quantitative characters namely, days to
50% flowering, plant height (cm), panicle
length (cm), number of productive tillers,
number of spikelets per panicle, 1000seed
weight (g) and grain yield per plant (g), kernel
length (mm), kernel breadth (mm) and L/B
ratio. Replicated mean data of each character
was subjected to analysis of variance
following Panse and Sukatme (1985).
Genotypic, phenotypic and environmental
variance along with heritability and genetic
advance were estimated for all the characters.
The phenotypic (PCV) and genotypic (GCV)
coefficients of variation, heritability (broad
sense) and genetic advance were estimated by
Int.J.Curr.Microbiol.App.Sci (2018) 7(12): 1288-1303
1290
the formulae suggested by Burton et al.,
(1952) and Johnson et al., (1955).
Results and Discussion
Among the eight qualitative characters studied
in morphological characterization of the
accessions (Table 1), three characters (Table
2) viz., absence of coleoptile colour, leaf
anthocyanin colouration and presence of leaf
ligule were common in all the 31 accessions of
O. glaberrima. Remaining five characters
were unique and distinct among the
accessions. The coleoptile was colourless in
all the accessions of O. glaberrima. Regarding
leaf characteristics, intensity of green colour
in leaves was light in 18 accessions (58.06%)
and medium green in the remaining 13
accessions. The anthocyanin colouration of
leaves was absent in all the accessions of O.
glaberrima, while all the 31 accessions of O.
glaberrima had the leaf ligule with split shape
in two accessions (EC 861804 and 861814 and
acute in the remaining 29 accessions. The
colour of ligule was also distinct across the
accessions as it was white in 26 and purple in
five accessions (EC861791, EC861796,
EC861805, EC861819 and EC861817). With
respect to the culm type, out of the 31
accessions of O. glaberrima, 27 accessions
(87.09%) showed erect culm, one accession
(EC861791) had semi erect culm, while
spreading culm was observed in three
accessions (EC861807, EC861812 and
EC861813). The O. glaberrima accessions
having erect type of culm can show lodging
resistance. Regarding the attitude of the flag
leaf blade, it was erect in six accessions (EC
861799, EC861804, EC861807, EC861810,
EC861814 and EC861816), semi-erect in 19
and horizontal in the remaining six accessions
(EC861792, EC816794, EC861797,
EC861784, EC861811 and EC861815). The
six accessions of O. glaberrima having erect
flag leaf can serve as donor lines for
improvement in rice breeding programmes.
For the panicle awn, nine accessions
(EC861795, EC861799, EC861803,
EC861812, EC861813, EC861814,
EC861815, EC861817 and EC861818)
recorded the presence of awn while it was
absent in the remaining 22 accessions. With
regard to the stem anthocyanin colouration, 13
accessions (41.93%) recorded the purple
colouration while 18 accessions did not show
anthocyanin colouration on stem. Two
accessions viz., EC861804, EC861805 were
found to be highly distinct as they possessed
distinct traits namely purple split ligule,
medium green leaves, erect flag leaf, presence
of awn along with lodging tolerance indicating
their usefulness as donors for crop
improvement programmes (Table 3).
Analysis of variance (Table 4) revealed
significant differences among the accessions
under study for all the ten traits indicating the
presence of considerable genetic variability in
the experimental material. A study of genetic
parameters (Table 5) revealed that phenotypic
and genotypic coefficients of variation were
high for number of productive tillers per plant
and spikelets per panicle The values of GCV
and PCV were moderate for days to 50 per
cent flowering, plant height, productive tillers,
1000 seed weight, grain yield per plant and
kernel length while low for panicle length,
kernel breadth and L/B ratio. These results are
in accordance with findings of Rusdiansyah et
al., (2017) for high GCV and PCV, Abebe et
al., (2017) for moderate GCV, PCV and
Edukondalu et al., (2017) for low GCV and
PCV in rice (O. sativa L.).High heritability
coupled with high genetic advance was
observed for days to 50 per cent flowering,
plant height and number of spikelets per
panicle indicated the preponderance of high
additive type of gene action in the inheritance
of these characters which can be further
improved by following simple selection
procedure as suggested by Abebe et al.,
(2017) (Fig. 1 and Table 6).
Int.J.Curr.Microbiol.App.Sci (2018) 7(12): 1288-1303
1291
Table.1 List of Oryza glaberrima accessions used in the present study
S.No
Origin
Biological status of accession
1
Guinea
Traditional cultivar/ Landrace
2
Guinea
Traditional cultivar/ Landrace
3
Guinea
Traditional cultivar/ Landrace
4
Guinea
Traditional cultivar/ Landrace
5
Guinea
Traditional cultivar/ Landrace
6
Guinea
Traditional cultivar/ Landrace
7
Guinea
Traditional cultivar/ Landrace
8
Guinea
Traditional cultivar/ Landrace
9
Guinea
Traditional cultivar/ Landrace
10
Guinea
Traditional cultivar/ Landrace
11
Guinea
Traditional cultivar/ Landrace
12
Guinea
Traditional cultivar/ Landrace
13
Guinea
Traditional cultivar/ Landrace
14
Guinea
Traditional cultivar/ Landrace
15
Guinea
Traditional cultivar/ Landrace
16
Guinea
Traditional cultivar/ Landrace
17
Guinea
Traditional cultivar/ Landrace
18
Guinea
Traditional cultivar/ Landrace
19
Guinea
Traditional cultivar/ Landrace
20
Guinea
Traditional cultivar/ Landrace
21
Guinea
Traditional cultivar/ Landrace
22
Guinea
Traditional cultivar/ Landrace
23
Guinea
Traditional cultivar/ Landrace
24
Guinea
Traditional cultivar/ Landrace
25
Guinea
Traditional cultivar/ Landrace
26
Guinea
Traditional cultivar/ Landrace
27
Guinea
Traditional cultivar/ Landrace
28
Guinea
Traditional cultivar/ Landrace
29
Guinea
Traditional cultivar/ Landrace
30
Malaysia
Wild
31
Malaysia
Wild
Int.J.Curr.Microbiol.App.Sci (2018) 7(12): 1288-1303
1292
Table.2 Characterization of 31 accessions of O. glaberrima for morphological traits
S.NO.
Accession
Coleoptile
colour
Intensity of
green colour
in leaves
Leaf
anthocyanin
colouration
Leaf
ligule
Culm type
Flag leaf
attitude
Panicle
awn
Stem
anthocyanin
colouration
1
EC 861784
colourless
light
absent
present
erect
horizontal
absent
absent
2
EC 861785
colourless
light
absent
present
erect
semi erect
absent
absent
3
EC 861786
colourless
light
absent
present
erect
semi erect
absent
absent
4
EC 861787
colourless
medium
absent
present
erect
semi erect
absent
absent
5
EC 861790
colourless
medium
absent
present
erect
semi erect
absent
absent
6
EC 861791
colourless
medium
absent
present
semi erect
semi erect
absent
absent
7
EC 861792
colourless
light
absent
present
erect
horizontal
absent
absent
8
EC 861794
colourless
medium
absent
present
erect
horizontal
absent
present
9
EC 861795
colourless
light
absent
present
erect
semi erect
present
present
10
EC 861796
colourless
light
absent
present
erect
semi erect
absent
absent
11
EC 861797
colourless
light
absent
present
erect
horizontal
absent
absent
12
EC 861799
colourless
light
absent
present
erect
erect
present
present
13
EC 861801
colourless
medium
absent
present
erect
semi erect
absent
present
14
EC 861802
colourless
light
absent
present
erect
semi erect
absent
present
15
EC 861803
colourless
light
absent
present
erect
semi erect
present
present
16
EC 861804
colourless
medium
absent
present
erect
erect
absent
absent
17
EC 861805
colourless
medium
absent
present
erect
semi erect
absent
present
18
EC 861807
colourless
medium
absent
present
spreading
semi erect
absent
present
19
EC 861808
colourless
medium
absent
present
erect
semi erect
absent
absent
20
EC 861809
colourless
medium
absent
present
erect
semi erect
absent
present
21
EC 861810
colourless
light
absent
present
erect
erect
absent
absent
22
EC 861811
colourless
light
absent
present
erect
horizontal
absent
absent
23
EC 861812
colourless
medium
absent
present
spreading
semi erect
present
absent
24
EC 861813
colourless
light
absent
present
spreading
semi erect
present
present
25
EC 861814
colourless
light
absent
present
erect
erect
present
present
26
EC 861815
colourless
light
absent
present
erect
horizontal
present
absent
27
EC 861816
colourless
medium
absent
present
erect
erect
absent
absent
28
EC 861817
colourless
medium
absent
present
erect
semi erect
present
absent
29
EC 861818
colourless
light
absent
present
erect
semi erect
present
present
30
EC 861819
colourless
light
absent
present
erect
semi erect
absent
present
31
EC 861820
colourless
light
absent
present
erect
semi erect
absent
absent
Int.J.Curr.Microbiol.App.Sci (2018) 7(12): 1288-1303
1293
Table.3 Frequency distribution of morphological traits in 31 accessions of O. glaberrima
S.No.
Character
Status
No. of
accessions
Frequency
Accessions
1
Coleoptile
colour
Colourless
31
100%
EC 861784, 861785, 861786, 861787, 861790,
861791, 861792, 861794, 861795, 861796,
861797, 861799, 861801, 861802, 861803,
861804, 861805, 861807, 861808, 861809,
861810, 861811, 861812, 861813, 861814,
861815, 861816, 861817, 861818, 861819, 861820
2
Intensity of
green colour in
leaves
Light
18
58.06%
EC 861784, 861785, 861786, 861792, 861795,
861796, 861797, 861799, 861802, 861803, 861810,
861811, 861813, 861814, 861815, 861818, 861819,
861820
Medium
13
41.93%
EC 861787, 861790, 861791, 861794, 861801,
861804, 861805, 861807, 861808, 861809, 861812,
861816, 861817
3
Leaf
anthocyanin
colouration
Present
0
0%
-
Absent
31
100%
EC 861784, 861785, 861786, 861787, 861790,
861791, 861792, 861794, 861795, 861796,
861797, 861799, 861801, 861802, 861803,
861804, 861805, 861807, 861808, 861809,
861810, 861811, 861812, 861813, 861814,
861815, 861816, 861817, 861818, 861819,
861820
Int.J.Curr.Microbiol.App.Sci (2018) 7(12): 1288-1303
1294
S.No.
Character
Status
No. of
accessions
Frequency
Accessions
4
Leaf ligule
Present
31
100%
EC 861784, 861785, 861786, 861787, 861790, 861791,
861792, 861794, 861795, 861796, 861797, 861799, 861801,
861802, 861803, 861804, 861805, 861807, 861808, 861809,
861810, 861811, 861812, 861813, 861814, 861815, 861816,
861817, 861818, 861819, 861820
Absent
0
0%
-
5
Culm type
Erect
27
87.09%
EC 861784, 861785, 861786, 861787, 861790, 861792,
861795, 861796, 861797, 861799, 861801, 861802, 861803,
861804, 861805, 861808, 861810, 861811, 861814, 861815,
861816, 861817, 861818, 861819, 861820
Semi erect
1
0.03%
EC 861791
Spreading
3
0.09%
EC 861807, 861812, 861813
6
Flag leaf
attitude
Erect
6
19.35%
EC 861799, 861804, 861807, 861810, 861814, 861816
Semi erect
19
61.29%
EC 861785,861786, 861787,861790, 861791,861795,
861796,861801, 861802,861803, 861805,861808,
Horizontal
6
19.35%
EC 861784, 86179, 861794, 86179, 861811, 861815
7
Panicle awn
Present
9
29.03%
EC 861795, 861799, 861803, 861812, 861813, 861814,
861815, 861817, 861818
Absent
22
70.96%
EC 861784, 861785, 861786, 861787, 861790, 861791,
861792, 861794, 861796, 861797, 861801, 861802, 861804,
861805, 861807, 861808, 861809, 861811, 861810, 861816,
861819, 861820
8
Stem
anthocyanin
colouration
Present
13
41.93%
EC 861794, 861795, 861799, 861801, 861802, 861803,
861805, 861807, 861809, 861813, 861814, 861818, 861819
Absent
18
58.06%
EC 861784, 861785, 861786, 861787, 861790, 861791,
861792, 861796, 861797, 861804, 861808, 861810, 861811,
861812, 861815, 861816, 861817, 861820
Int.J.Curr.Microbiol.App.Sci (2018) 7(12): 1288-1303
1295
Table.4 Analysis of variance for yield and yield attributing traits in rice Oryza glaberrima
accessions
S.
No.
Character
Replication
(d.f.=1)
Treatment
(d.f.=30)
Error
(d.f.=30)
1
Days to 50% flowering
41.30
283.00**
12.58
2
Plant height (cm)
0.16
476.159**
57.39
3
Number of productive tillers
1.19
36.29**
2.21
4
Panicle length (cm)
3.71
8.64**
3.63
5
No. of spikelets per panicle
71.85
2257.44**
279.08
6
1000 grain weight (g)
17.80
16.12**
5.06
7
Grain yield per plant (g)
1.45
3.54**
0.36
8
Kernel length (mm)
0.11
0.48**
0.18
9
Kernel breadth (mm)
0.03
0.09**
0.02
10
L/B ratio
0.01
0.13**
0.05
** Significant at 1% level * Significant at 5% level
Table.5 Magnitude of variability, heritability and genetic advance for yield and yield attributing
traits in Oryza glaberrima accessions
Characters
PCV
(%)
GCV
(%)
Heritability in
broad
sense(h2)(%)
Genetic
Advance
(at 5%)
Days to 50% flowering
10.38
9.93
91.49
22.91
Plant height (cm)
12.32
10.92
78.49
26.41
No. of productive tillers per plant
34.38
32.35
88.51
8.00
Panicle length (cm)
9.33
6.35
40.84
2.08
No. of spikelets per panicle
29.98
26.48
77.99
57.22
1000 grain weight (g)
18.45
13.33
52.19
3.4
Grain yield per plant (g)
16.58
14.98
81.63
2.35
Kernel length (mm)
16.36
15.33
87.86
2.51
Kernel breadth (mm)
8.55
6.13
51.54
0.26
L/B ratio
10.34
7.00
45.86
0.28
Int.J.Curr.Microbiol.App.Sci (2018) 7(12): 1288-1303
1296
Table.6 Mean performance of O.glaberrima accessions for yield and yield attributing traits
S.NO.
Accession
Days to
50%
flowering
Plant
height
(cm)
Productive
tillers
Panicle
length
(cm)
Spikelets/
Panicle
1000
seed
weight
(g)
Yield/
Plant
(g)
Kernel
length
(mm)
Kernel
breadth
(mm)
L/B
ratio
1
EC 861784
126.0
127.0
12.0
26.7
110.0
15.9
8.2
7.2
2.6
2.7
2
EC 861785
125.0
150.9
14.0
29.1
102.0
14.8
7.2
8.0
3.4
2.3
3
EC 861786
139.0
138.8
14.0
24.2
110.0
13.7
8.1
7.8
2.7
2.8
4
EC 861787
120.0
128.5
8.0
24.0
159.0
15.5
7.2
7.3
3.0
2.4
5
EC 861790
137.0
122.2
14.0
27.0
119.0
16.3
9.7
8.3
3.0
2.7
6
EC 861791
146.0
122.8
13.0
26.0
87.0
11.3
6.5
7.5
2.7
2.7
7
EC 861792
126.0
132.9
14.0
23.4
107.0
14.6
7.1
8.7
2.8
3.0
8
EC 861794
116.0
133.3
11.0
28.2
145.0
17.6
8.5
7.9
2.7
2.8
9
EC 861795
119.0
144.6
9.0
23.3
120.0
18.7
9.7
9.0
3.0
3.0
10
EC 861796
113.0
144.2
10.0
27.5
121.0
22.0
7.3
8.4
2.8
2.9
11
EC 861797
123.0
122.7
8.0
25.4
113.0
17.6
9.0
7.4
2.9
2.5
12
EC 861799
117.0
132.7
15.0
27.1
94.0
19.2
9.1
8.3
2.9
2.8
13
EC 861801
121.0
136.9
7.0
27.3
98.0
16.2
9.1
8.2
3.1
2.6
14
EC 861802
120.0
133.8
10.0
27.7
108.0
19.3
9.4
8.3
2.9
2.8
15
EC 861803
114.0
140.6
13.0
23.8
84.0
15.8
8.3
8.2
2.7
3.0
16
EC 861804
100.0
138.8
16.0
23.0
87.0
22.8
8.2
8.3
3.2
2.5
17
EC 861805
140.0
140.1
9.0
24.4
80.0
12.1
6.5
8.3
3.2
2.5
18
EC 861807
112.0
144.6
15.0
24.3
113.0
18.2
8.2
8.1
3.2
2.5
19
EC 861808
114.0
130.7
14.0
23.3
118.0
18.4
7.9
8.5
3.0
2.8
20
EC 861809
104.0
136.5
12.0
29.3
101.0
18.1
10.8
8.4
2.9
2.9
21
EC 861810
116.0
118.0
12.0
24.2
138.0
19.2
8.9
8.7
3.0
2.9
22
EC 861811
112.0
139.3
8.0
24.7
110.0
19.0
10.1
8.5
2.9
2.9
23
EC 861812
111.0
124.3
10.0
22.6
110.0
22.1
7.1
8.0
3.0
2.6
24
EC 861813
131.0
128.2
15.0
22.8
110.0
16.1
12.8
8.8
2.4
3.6
25
EC 861814
109.0
138.2
7.0
24.4
102.0
20.7
7.9
8.5
2.8
2.9
26
EC 861815
104.0
150.8
14.0
23.9
115.0
15.8
7.8
9.0
2.8
3.2
27
EC 861816
86.0
117.3
7.0
23.0
117.0
19.8
7.8
8.5
2.8
3.0
28
EC 861817
106.0
147.5
11.0
25.7
104.0
19.3
8.2
8.1
2.7
2.9
29
EC 861818
109.0
155.2
7.0
23.6
126.0
17.9
8.3
8.2
2.8
2.8
30
EC 861819
103.0
147.2
8.0
22.5
94.0
20.0
8.0
8.7
2.9
3.0
31
EC 861820
118.0
152.0
13.0
27.2
91.0
19.8
6.8
9.2
2.9
3.1
Int.J.Curr.Microbiol.App.Sci (2018) 7(12): 1288-1303
1297
Fig.1 Bar graph for morphological characterization of 31 accessions of O. glaberrima
Int.J.Curr.Microbiol.App.Sci (2018) 7(12): 1288-1303
1298
Plate.3 Variation in ligule colour and shape
Int.J.Curr.Microbiol.App.Sci (2018) 7(12): 1288-1303
1299
Plate.4 Variation in culm attitude
Erect
Semi Erect
Spreading
Int.J.Curr.Microbiol.App.Sci (2018) 7(12): 1288-1303
1300
Plate.5 Variation in attitude of flag leaf
Erect
Semi Erect
Horizontal
Int.J.Curr.Microbiol.App.Sci (2018) 7(12): 1288-1303
1301
Plate.6a Variation in panicle awn
Plate.6b Variation in stem anthocyanin colouration
Short awned
Long awned
Awnless
Anthocyanin pigmentation on stem
Absence of Anthocyanin on stem
Int.J.Curr.Microbiol.App.Sci (2018) 7(12): 1288-1303
1302
Plate.7 Variation in leaf intensity of green colour
The high estimates of heritability coupled with
low genetic advance for no.of productive tillers
per plant, grain yield per plant, and kernel
length indicated the presence of non-additive
gene effects. In the present study, three superior
accessions, viz., EC861785, EC861804 and
EC861813were found to be potential enough to
be used as parents in various breeding
programmes. These accessions recorded highest
values for panicle length, productive tillers and
grain yield per plant and hence their utilization
in combination breeding may help in generating
high yielding varieties by pyramiding all the
favourable genes and keeping in view of the
facts, much attention needs to be given for the
components with high GCV, PCV and high
heritability coupled with high genetic advance
during selection for the further improvement of
the remaining accessions.
In conclusion, among the eight morphological
traits studied in the 31 accessions of O.
glaberrima, three characters viz., absence of
coleoptile colour, absence of leaf anthocyanin
colouration and presence of leaf ligule were
reported in all the 31 accessions. Remaining
five characters were unique and distinct among
the accessions. Out of 31 accessions of O.
glaberrima, two accessions viz., EC 861804, EC
861805 were found to be highly distinct as they
possessed distinct traits namely purple split
ligule, medium green leaves, erect flag leaf,
presence of awn along with lodging tolerance.
Adequate genetic variability in the 31
accessions of O. glaberrima was observed.
The magnitude of PCV and GCV was high for
number of productive tillers per plant, while
high heritability coupled with high genetic
advance was observed for days to 50 per cent
flowering and number of spikelets per panicle.
The accessions EC861785, EC861804 and
EC861813 can be used as parental material in
future rice breeding programme as they have
recorded highest values for the important yield
traits viz., for panicle length, productive tillers
and grain yield per plant. The present study
revealed sufficient genetic variability for yield
related traits which could be exploited for
genetic improvement of rice cultivars (Oryza
sativa L).
Light Green
Medium Green
Int.J.Curr.Microbiol.App.Sci (2018) 7(12): 1288-1303
1303
References
Abebe, T., Alamerew, S and Tulu, L. 2017.
Genetic variability, heritability and
genetic advance for yield and its related
traits in rainfed lowland rice (Oryza
sativa L.) genotypes at Fogera and
Pawe, Ethiopia. Advanced Crop Science
and Technology.5: 272.
Ahmad.,Qayyum., Sahibzada., Saleem.,
Ghaffar., Mehnaz and Farhad. 2011.
Genetic diversity analysis for yield and
other parameters in maize (Zea mays L.)
genotypes. Asian Journal of Agricultural
Sciences. 3.
Burton, G.W and DeVane, E.H. 1952.
Estimating heritability in tall fescue
(Festuca arundinacea) from replicated
clonal material. Agronomy Journal. 19:
45.
Edukondalu, B., Ram Reddy, V., Shobha Rani,
T., ArunaKumari, Ch and Soundharya, B.
2017. Studies on variability, heritability,
correlation and path analysis for yield,
yield attributes in rice (Oryza sativa
L.).International Journal of Current
Microbiology and Applied Sciences.6
(10): 2369-2376.
Fonfana, B and Cloutier, S. 2008. Assesment of
molecular diversity with QTLs for
preharvest sprouting resistance in wheat
using microsatellite markers. Genome. 51:
375-386.
Indiastat.Agriculture Production Statistical
Database.2016-17. http://www.indiastat.
com.
Johnson, H.W., Robinson, H.F and Comstock,
R.E. 1955. Estimation of genetic and
environmental variability in soybean.
Agronomy Journal. 47: 314318.
Najeeb, S., Rather, A.G., Parray, G.A., Sheikh,
F.A and Razvi, S.M. 2009.Studies on
genetic variability, genotypic correlation
and path coefficient analysis in maize
under high altitude temperate ecology of
Kashmir. Maize Genetics Cooperation
Newsletter. 83: 1- 8.
Panse, V.G and Sukhatme, P.V. 1985.
Statistical methods for Agriculture
workers. Indian council of Agricultural
research Publication. 87-89.
Raut, V.M. 2003. Qualitative genetics of
Soyabean. Soybean Research. 1:1-28.
Rout B., Sridhar M., Muralidhara, B., Kamal
Nath Reddy, KR., Sundaram R.M.,
Anantha M.S., Senguttuvel, P.,
SubbaRao, L.V, Padmavathi, G.,
Ranganath, H.K., Fiyaz, A.R., Jyothi, B.,
Suvarna Rani, C., Kalyani, M.B.,
Bidyasagar Mandal and Gireesh, C. 2017.
Characterization of genetic diversity
among wild rice accessions using genome
specific In-Del markers. Journal of Rice
Research.10 (2): 11-17.
Rusdiansyah, M., Subiono, T., Sunaryo, W.,
Suryadi, A., Sulastri, G and Anjasmara, S.
2017.The genetic diversity and
agronomical characters of local cultivars
of tidal rice in East Kalimantan,
Indonesia. Biodiversitas. 18:1289-1293.
Sarla, N and Swamy, B.P.M. 2005.Oryza
glaberrima: A source for improving
Oryza sativa. Current science. 89 (6):
955-963.
Waqar, M.F., Malik., Rashid, M., Munir, M and
Akram, Z. 2008. Evaluation and
estimation of heritability and genetic
advancement for yield related attributes in
wheat lines. Pakistan Journal of Botany.
40 (4):1699-1702.
How to cite this article:
Ishwarya Lakshmi, V.G., C. Gireesh, M. Sreedhar, S. Vanisri, P.S. Basavaraj, B. Muralidhara, M.S.
Anantha, G. Padmavathi, A.R. Fiyaz, B. Jyothi, C. Suvarna Rani, Bidyasagar Mandal and Subba
Rao, L.V. 2018. Characterization of African Rice Germplasm for Morphological and Yield
Attributing Traits. Int.J.Curr.Microbiol.App.Sci. 7(12): 1288-1303.
doi: https://doi.org/10.20546/ijcmas.2018.712.159
ResearchGate has not been able to resolve any citations for this publication.
Article
Full-text available
Availability of local rice germplasm is very important for plant breeding, especially in the improvement of the genetic diversity. The objectives of the research were to study the genetic diversity and agronomical characters of local characters of tidal rice in East Kalimantan. The research was conducted from May to October 2014 in the village of Sidomulyo Anggana District, Kutai Kertanegara District, East Kalimantan. The experiment was arranged in a randomized complete block design (RCBD) with five replications. Eight selected local tidal rice cultivars from East Kalimantan (Pandan Ungu, Kambang, Amas, Roti, Pudak, Sikin Merah, Sikin Putih and Popot) were used in this experiment as plant materials. The results showed that there was wide genetic diversity among local tidal-rice cultivars selected from East Kalimantan. Number of productive tillers, the percentage of empty grain per panicle, 1000 grain weight and yield per hectare can be used as selection criteria for local lowland rice cultivars from East Kalimantan because they have a high value of heritability (h2bs), phenotype and genotype and coefficient of variation (PCV and GCV). Pandan Ungu, Kambang, Amas, Roti and Sikin Merah cultivars potential to be used as parents in the breeding program.
Article
Full-text available
The present study consists of 36 rice genotypes that were evaluated at two locations, namely Fogera and Pawe to study genetic variability, heritability and genetic advance for grain yield and 13 yield associated traits. The experiment was conducted using 6 × 6 simple lattice design across two locations with two replications during the 2015/2016 main cropping season. The combined analysis of variance revealed statistically significant differences (p<0.05) indicating the existence of genetic variability among the 36 genotypes for all the traits studied. Genotype × location interactions were significant for days to maturity, plant height, panicle length, culm length, flag leaf length, number of filled grain per panicle, number of total spikelet per panicle, days to heading, biomass yield, grain yield and harvest index. Significant differences were observed for grain yield that ranged from 6759.00 to 2886.00 kg ha-1 with overall mean value of 5370.0 kg ha-1. Higher PCV and GCV values were exhibited by plant height, culm length, number of unfilled grain per panicle, biomass yield and grain yield, which suggests the possibility of improving this trait through selection. The highest heritability was recorded for culm length followed by plant height, biomass yield and panicle length. High to medium heritability coupled with high GCV and high genetic advance as percentage of means were exhibited for plant height, biomass yield, grain yield and number of unfilled grain per panicle. High genetic advances as percent of means were recorded by plant height, culm length, biomass yield, grain yield and number of unfilled grain per panicle.
Article
Full-text available
African rice Oryza glaberrima (2n = 24, AA) has many unique traits such as weed competitiveness, drought tolerance and the ability to grow under low input conditions. These traits have been recently combined with high yield of Oryza sativa to develop NERICA (NEw RIce for AfriCA). The new rice varieties are high-yielding, drought- and pest-resistant and are uniquely adapted to the growing conditions of West Africa. The major problems in combining useful traits of both cultivated Oryza species are sexual incompatibility and hybrid sterility. Reproductive barriers between the two species have been studied and genetic models based on sporogametophytic interactions proposed. The availability of a molecular linkage map of O. glaberrima and molecular markers linked to sterility loci would aid in the introgression of desirable traits and elimination of sterility loci quickly. The use of O. glaberrima in rice breeding is challenging, but promising. The genetic base of Asian rice O. sativa can be widened for obtaining higher yields under adverse conditions and using non-transgenic approaches.
Article
Full-text available
Ten wheat genotypes were evaluated for estimation of heritability and genetic advance of various yield related parameters viz. spike length, number of spikes per plant, number of spikelets per spike, number of grains per spike, 1000 grain weight and grain yield per plant. Among all the studied characters, number of grains per spike, 1000 grain weight and grain yield per plant showed high values of heritability coupled with high genetic advance that result in prevaing suitable conditions for selection.
Article
RiCENT studies on a number of characters of soybeans have been directed toward estimation of heritability, that is, the fraction of variance in phenotypic expression that arises from genetic effects. However, the different methods employed do not necessarily estimate the same thing. For example, variance and regression methods of estimating heritability of F2 plant differences estimate the same thing only if all gene effects are additive. The nature of the selection units (plant, plot, mean of several plots, etc.) and sampling errors also influence greatly the magnitude of heritability estimates. Therefore, any meaningful comparison of the estimates obtained in different situations must include a careful evaluation of the methods and materials employed
Assesment of molecular diversity with QTLs for preharvest sprouting resistance in wheat using microsatellite markers
  • Fonfana
  • S Cloutier
Fonfana, B and Cloutier, S. 2008. Assesment of molecular diversity with QTLs for preharvest sprouting resistance in wheat using microsatellite markers. Genome. 51: 375-386.
Agriculture Production Statistical Database
  • Indiastat
Indiastat.Agriculture Production Statistical Database.2016-17. http://www.indiastat. com.