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1. Supplementary fig. 1
Morphological observation of rats
Compared with the normal control group, rats in YDCS model group appeared to have an
addiction of lying, messy and dry hair, squinting, listless, pale and partial darkness on lips and
paws, clear urine in large amount, loose stools, etc.
2. Supplementary fig. 2
Morphological observation of rats
After 7 days of treatment with RA water abstract, the overall condition of the animals
improved significantly. The total activity, hair became smoother and shiner, good mental health
and increased activity, Lips, stools forming and became moderate, nails turned white and pink.,
improvement in urine color, etc.
3. Supplementary fig. 3
The change of PPAR signal pathway in YDCS model
The PPAR signal pathway is an important pathway for metabolism and immunity, in
YDCS pathological process, body's energy metabolism dysregulation represented by glucose
metabolism, lipid metabolism, and amino acid metabolism reduction, reflect in PPAR signal
pathway. It is closely related to with PPAR signaling pathway and downregulation of the target
genes CPT-1, FABP4.
4. Supplementary fig. 4
The change of PPAR Signaling Pathway in RAWA treatment group
Combining with the transcriptome results, the effect of RA membranaceus on energy
metabolism may be due to the action on PPAR signaling pathway, resulting in the upregulation
of the target gene CPT-1 and FABP4. RA can also enhance production of heat and promote the
reduced energy metabolism in the YDCS model rats. These benefits are the specific
manifestation of its warming effect. RAWA upregulates lipid metabolism represented by ap2
(Fabp4) under the action of cytochrome P450 family member Cyp1a1 in vivo. Activation of
PPAR signaling pathway (path: rno03320).