Objective: Smilax myosotiflora is known locally as 'ubi jaga, 'akar ali', 'itah besi' and others, is a climber plant which is her-baceous and nutritious to be consumed. S. myosotiflora is among the well-known ingredients in several Malay traditional medicines and preparations of aboriginal herb. In addition, it is regarded as the aphrodisiac plant according to previous studies. S. myosotiflora ... [Show full abstract] has been consumed these days to enhance the sexual libido in men performance. This study is to evaluate the cyto-toxic effect against the brine shrimp nauplii of methanolic and petroleum ether extract of S.myosotiflora. Design: The methanol and petroleum ether extracts of S.myosotiflora were tested for its toxicity using brine shrimp lethality test (BSLT). Materials and methods: The percentage (%) of mortality of the brine shrimp nauplii was calculated for each dilution concentration. Then, LC50 was established. Results: The brine shrimp lethality test of S. myosotiflora extracts exhibited no significant toxicity against Artemia salina. S. myosotiflora extract has high LC50. Conclusion: The S.myosotiflora extract with high LC50 indicated that this plant is not toxic to brine shrimp. So, it may be safe for human consumption as medicinal plants.