IN-VITRO CULTURING OF HUMAN EMBRYOS. TIME-LAPSE TECHNOLOGIES AND EMBRYOSCOPE

Abstract

In-vitro culturing and selection of human embryos are crucial stages in the applied assisted reproduction techniques. The routinely applied standard methods of embryo assessment are based on morphological criteria at discrete time points. Efforts aimed at improving pregnancy rates in IVF procedures are focused on the search for additional biomarkers for more accurate embryo selection. The EmbryoScope is an incubator with an integrated time-lapse system, allowing a detailed observation of the embryo in real time, from the moment of fertilization to its transfer to the uterus or to cryopreservation. Time-lapse technology represents a powerful tool in assisted reproduction for evaluating the time of embryo development. Time-lapse recording introduces several important morphokinetic parameters for embryo evaluation. This additional knowledge helps us to implement an individual patient treatment approach, to optimise culturing environment, and as a final result to improve implantation rates.

Full text

INTERNATIONAL SCIENTIFIC CONFERENCE “KLIMENT’S DAYS”
8-9 NOvEMbER 2018 SOFIA FACuLTY OF bIOLOgY
23
IN-VITRO CULTURING OF HUMAN EMBRYOS. TIME-LAPSE
TECHNOLOGIES AND EMBRYOSCOPE
STEFKA NIKOLOVA, M. IBRYAMOVA, I. IVANOVA,
K. NIKOLOVA, D. PARVANOV, G. STAMENOV, T. CHAUSHEV
Nadezhda,Women’s Health Hospital, Soa, Bulgaria
*Corresponding author: s.nikolova@nadezhdal.bg
Keywords: time-lapse, morphokinetic biomarkers, human embryos
In-vitro culturing and selection of human embryos are crucial stages in the
applied assisted reproduction techniques. The routinely applied standard
methods of embryo assessment are based on morphological criteria at discrete
time points. Efforts aimed at improving pregnancy rates in IVF procedures
are focused on the search for additional biomarkers for more accurate embryo
selection.
The EmbryoScope is an incubator with an integrated time-lapse system,
allowing a detailed observation of the embryo in real time, from the moment
of fertilization to its transfer to the uterus or to cryopreservation. Time-lapse
technology represents a powerful tool in assisted reproduction for evaluating
the time of embryo development. Time-lapse recording introduces several
important morphokinetic parameters for embryo evaluation. This additional
knowledge helps us to implement an individual patient treatment approach, to
optimise culturing environment, and as a nal result to improve implantation
rates.
PLENARY LECTURES

Sofia University St. Kliment Ohridski
Faculty of Biology
National Science Fund of Ministry
of Education and Science of Bulgaria
FUNDED BY:
PROJECT № ДПМНФ 01/30 2018 NSF MINISTRY OF EDUCATION AND SCIENCE;
PROJECT № 80-10-50/2018 SOFIA UNIVERSITY;
PROJECT № 3022/2018 SCIENTIFIC RESEARCH DEPARTMENT, SOFIA NIVERSITY
INTERNATIONAL
SCIENTIFIC CONFERENCE
“KLIMENT’S DAYS”
8–9 NOVEMBER 2018
A B S T R A C T S

Sofia University St. Kliment Ohridski
Faculty of Biology
National Science Fund of Ministry
of Education and Science of Bulgaria
INTERNATIONAL
SCIENTIFIC CONFERENCE
“KLIMENT’S DAYS”
8–9 NOVEMBER 2018
A B S T R A C T S
SOFIA 2018

HONORARY CHAIRMAN
Prof. Mariela Odjakova
CHAIRMAN
Assoc. Prof. STOYAN SHISHKOV, Dean of Faculty of Biology
SCIENTIFIC COMMITTEE
Prof. Roumen Pankov (Bulgaria)
Prof. Adele Muscolo (Italy)
Prof. Yana Topalova (Bulgaria)
Prof. Mitko Mladenov (FYR Macedonia)
Prof. Maya Stoyneva-Gartner (Bulgaria)
Prof. Gerd-Joachim Krauss (Germany)
Prof. Hristo Gagov (Bulgaria)
Prof. Zoran Popovski (FYR Macedonia)
Prof. Plamen Mitov (Bulgaria)
Prof. Yalcin Kaya (Turkey)
Prof. Svetla Petrova (Bulgaria)
Prof. Georg Gartner (Austria)
Prof. Daniela Simeonovska-Nikolova (Bulgaria)
Assos. Prof. Vyara Mamatarkova (Bulgaria)
Assos. Prof. Valentina Ganeva (Bulgaria)
Assos. Prof. Svetoslav Dimov (Bulgaria)
Assos. Prof. Ganka Chaneva (Bulgaria)
Assos. Prof. Ventsislava Petrova (Bulgaria)
Assos. Prof. Asya Asenova (Bulgaria)
Assos. Prof. Anelya Kenarova (Bulgaria)
ORGANIZING COMMITTEE:
Assoc. Prof. Dilyana Nikolova
Assoc. Prof. Ivan Traykov
Assoc. Prof. Anita Tosheva
Assoc. Prof. Yana Evstatieva
Assoc. Prof. Lyuben Zagorchev
Assoc. Prof. Denitsa Teofanova
Assist. Prof. Atanas Grozdanov
Assist. Prof. Daniel Todorov
Secretary: Mrs. Mariana Modreva
Webmaster: Mr. Dimitar Milanov
http://www.biofac.info/
Funded by:
Project № ДПМНФ 01/30 2018 NSF, Ministry of Education and Science;
Project № 0-10-50/2018 Soa University;
Project № 3022/2018 Scientic Research Department, Soa University
ISBN 978-954-07-4544-2