Evaluation of the Effect of Consciousness Energy Healing Treatment on the Physicochemical and Thermal Properties of Selenium

Abstract

Selenium is an essential micronutrient required for healthy metabolism, as well as prevention, and treatment of selenium deficiency diseases. The experiment aimed to evaluate the influence of the Trivedi Effect®-Consciousness Energy Healing Treatment on the physicochemical and thermal properties of selenium using modern analytical techniques. The selenium sample was divided into two parts, one part of the test sample was called the control sample, while the second part of the test sample received the Biofield Treatment remotely by a renowned Biofield Energy Healer, Gopal Nayak, and was called the treated sample. The particle size values were significantly decreased by 37.69% (d10), 14.36% (d50), 4.31% (d90), and 11.58% [D(4,3)], hence, the specific surface area was significantly increased by 33.64% in the treated sample compared to the control sample. The PXRD peak intensities and crystallite sizes were significantly altered ranging from 5.23% to 100% and 75% to 111.7%, respectively; whereas 7.81% significantly decreased the average crystallite size in the treated sample than the control sample. The latent heat of fusion of the treated sample was significantly increased by 12.37% compared with the control sample. The results suggested that the Trivedi Effect® might generate a new polymorphic form of selenium which would offer better solubility, bioavailability and be thermally more stable compared with the control sample. The Biofield Treated selenium would be more useful to design novel nutraceutical/pharmaceutical formulations and might offer an enhanced therapeutic response against cardiovascular disease, cancer, neuromuscular disorders, diabetes, stress, aging, male infertility, viral diseases, degenerative ailments, etc.

Full text

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Evaluation of the Effect of Consciousness Energy Healing Treatment on the Physicochemical and
Thermal Properties of Selenium
Gopal Nayak 1, Mahendra Kumar Trivedi 1, Alice Branton 1, Dahryn Trivedi 1, Snehasis Jana 2, *
1 Trivedi Global, Inc., Henderson, USA.
2 Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, India.
Abstract
Selenium is an essential micronutrient required for healthy metabolism, as well as prevention, and
treatment of selenium deficiency diseases. The experiment aimed to evaluate the influence of the Trivedi
Effect®-Consciousness Energy Healing Treatment on the physicochemical and thermal properties of selenium
using modern analytical techniques. The selenium sample was divided into two parts, one part of the test
sample was called the control sample, while the second part of the test sample received the Biofield Treatment
remotely by a renowned Biofield Energy Healer, Gopal Nayak, and was called the treated sample. The particle
size values were significantly decreased by 37.69% (d10), 14.36% (d50), 4.31% (d90), and 11.58% [D(4,3)],
hence, the specific surface area was significantly increased by 33.64% in the treated sample compared to the
control sample. The PXRD peak intensities and crystallite sizes were significantly altered ranging from 5.23% to
100% and 75% to 111.7%, respectively; whereas 7.81% significantly decreased the average crystallite size in
the treated sample than the control sample. The latent heat of fusion of the treated sample was significantly
increased by 12.37% compared with the control sample. The results suggested that the Trivedi Effect® might
generate a new polymorphic form of selenium which would offer better solubility, bioavailability and be thermally
more stable compared with the control sample. The Biofield Treated selenium would be more useful to design
novel nutraceutical/pharmaceutical formulations and might offer an enhanced therapeutic response against
cardiovascular disease, cancer, neuromuscular disorders, diabetes, stress, aging, male infertility, viral diseases,
degenerative ailments, etc.
DOI : 10.14302/issn.2377-2549.jndc-18-1933
Corresponding Author: Snehasis Jana, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, India.
Tel: +91-022-25811234; Email: publication@trivedieffect.com
Running Title: Impact of Consciousness Energy Treatment on Selenium
Keywords: Selenium, The Trivedi Effect®, Consciousness Energy Healing Treatment, Complementary and
Alternative Medicine, Particle size, Surface area, PXRD, DSC
Received: Aug 22, 2018 Accepted: Sep 20, 2018 Published: Sep 24, 2018
Editor: Weihe Zhang, The University of North Carolina at Chapel Hill, USA.
ISSN NO: 2377-2549
Research Arcle DOI : 10.14302/issn.2377-2549.jndc-18-2315

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Introduction
Selenium (Se) is an essential trace element
nutrient for humans and animals. It regulates a healthy
metabolism and inhibits the toxic effects of heavy metals
in the body [1]. The rich natural sources for Se are
meat, fish, mushrooms, cereals, nuts, etc. This can be
obtained from mineral supplements [1,2]. It is one of
the key components of unusual amino acids
selenocysteine and selenomethionine; selenium
enzymes, and about 30 selenoproteins [3]. It is a potent
antioxidant, which protects against oxidative damage,
infections, nervous system; plays critical roles in
reproduction, DNA synthesis, and thyroid hormone
metabolism [1,4,5]. A poor selenium containing diet
and/or genetic problems may lead to selenium deficiency
in the body [6]. Se deficiency in the body is responsible
for the critical pathophysiology of many diseases, i.e.,
cancer, diabetes, male infertility, muscle disorders,
neurological disorders, cardiovascular disease,
degenerative ailments, viral diseases, etc. [7-9].
Therefore, Se is recommended as a daily supplement in
a number of countries. But, excess intake of Se may
cause adverse health effects [9,10]. It is absorbed by
the body in the form selenite which is more than 80
percent.
The physicochemical properties of any
pharmaceutical/nutraceutical compound play a crucial
role in its stability, solubility, bioavailability, and
therapeutic efficacy in the body [11]. The biggest
challenge for pharmaceutical scientists is improving the
quality of pharmaceutical/nutraceutical compounds for
better therapeutic efficacy. Scientifically, the Trivedi
Effect®-Consciousness Energy Healing Treatment
(Biofield Energy Healing Treatment) has been proven to
have a significant impact on the particle size, surface
area, thermal properties, and bioavailability of
pharmaceutical and nutraceutical compounds [12-14].
The Trivedi Effect® is a natural and the only scientifically
proven phenomenon in which an expert can harness this
inherently intelligent energy from the Universe and
transmit it anywhere on the planet through the possible
mediation of neutrinos [15]. “Biofield” is a unique,
infinite, para-dimensional electromagnetic field exists
surrounding the body, originating from the continuous
movements of the charged particles, ions, cells, blood/
lymph flow, brain functions, heart function, etc. This
Biofield Energy Therapy (energy medicine) has been
reported to have substantial outcomes against various
disease conditions and to maintain the overall quality of
life [15,16]. The National Institutes of Health/National
Center for Complementary and Alternative Medicine
(NIH/NCCAM) recommend and included the Energy
therapy under the Complementary and Alternative
Medicine (CAM) category along with other therapies,
medicines and practices such as Ayurvedic medicine,
naturopathy, homeopathy, Qi Gong, Tai Chi, yoga,
chiropractic/osteopathic manipulation, meditation,
massage, acupuncture, acupressure, hypnotherapy,
Reiki, Rolfing structural integration, mindfulness,
aromatherapy, cranial sacral therapy, applied prayer,
etc. The CAM has been accepted by the most of the U.S.
population with several advantages [17,18]. Similarly,
the Trivedi Effect® Treatment also has a significant
impact on the characteristic properties of the metals,
ceramics, and polymers, organic compounds, crops,
livestock, microorganisms, and cancer cells [19-29].
These outstanding experimental results motivated the
authors to determine the impact of the Trivedi Effect®-
Consciousness Energy Healing Treatment on the
physicochemical and thermal properties of selenium
using particle size analysis (PSA), powder X-ray
diffraction (PXRD), and differential scanning calorimetry
(DSC).
Materials and Methods
Chemicals and Reagents
The selenium (Se) powder sample was procured
from Sigma Aldrich, USA and the other chemicals
required during the experiments were of the analytical
standard available in India.
Consciousness
Energy Healing Treatment
Strategies
The test sample Se powder used in the
experiment was divided into two parts. One part of the
Se powder sample was received the Trivedi
Effect®-Consciousness Energy Healing Treatment
remotely under standard laboratory conditions for 3
minutes by the renowned Biofield Energy Healer, Gopal
Nayak, India, known as the treated sample. The second
part of the sample did not receive the Biofield Energy
Treatment and was called the control sample. Further,
the control sample was treated with “sham” healer for

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the comparison purposes. The “sham” healer did not
have any knowledge about the Biofield Energy
Treatment. The Biofield Energy Treated sample and
untreated Se powder sample were both kept in sealed
conditions and characterized using PSA, PXRD, and DSC
analytical techniques.
Characterization
Particle Size Analysis (PSA)
The particle size analysis of Se powder was
performed with the help of Malvern Mastersizer 2000, of
the UK, with a detection range between 0.01 µm to
3000 µm using the wet method [30, 31]. The sample
unit (Hydro MV) was filled with a sunflower oil
dispersant medium and the stirrer operated at 2500
rpm. Particle size distribution analysis of Se powder was
performed to obtain the average particle size. Where, d
(0.1) μm, d(0.5) μm, d(0.9) μm represent particle
diameter corresponding to 10%, 50%, and 90% of the
cumulative distribution. D(4,3) represents the average
mass-volume diameter, and SSA is the specific surface
area (m2/g). The calculations were done by using
software Mastersizer Ver. 5.54.
The percent change in particle size (d) for Se
powder at below 10% level (d10), 50% level (d50), 90%
level (d90), and D(4,3) was calculated using the following
equation 1:
(1)
Where dControl and dTreated are the particle sizes (μm) at
below 10% level (d10), 50% level (d50), and 90% level
(d90) of the control and the Biofield Energy Treated
samples, respectively.
The percent change in surface area (S) was
calculated using the following equation 2:
(2)
Where SControl and STreated are the surface area of the
control and the Biofield Energy Treated Se, respectively.
Powder X-ray Diffraction (PXRD) Analysis
The PXRD analysis of Se powder sample was
executed with the help of Rigaku MiniFlex-II Desktop
X-ray diffractometer (Japan) [32, 33]. The Cu Kα
radiation source tube output voltage and output current
were 30 kV and 15 mA, respectively. Scans were
performed at room temperature. The size of individual
crystallites was calculated from PXRD data using the
Scherrer’s formula (3)
G = kλ/βcosθ (3)
Where k is the equipment constant (0.94), G is the
crystallite size in nm, λ is the radiation wavelength
(0.154056 nm for Kα1 emission), β is the full-width at
half maximum (FWHM), and θ is the Bragg angle [34].
The percent change in crystallite size (G) of Se
was calculated using the following equation 4:
(4)
Where GControl and GTreated are the crystallite size of the
control and the Biofield Energy Treated samples,
respectively.
Differential Scanning Calorimetry (DSC)
The DSC analysis of Se powder sample was
performed with the help of DSC Q200, TA instruments.
Sample of ~1-2 mg was loaded to the aluminium sample
pan at a heating rate of 10ºC/min from 30°C to
350°C [30, 31]. The % change in melting point (T) was
calculated using the following equation 5:
(5)
Where TControl and TTreated are the melting point of the
control and the Biofield Energy Treated samples,
respectively.
The percent change in the latent heat of fusion
(ΔH) was calculated using following equation 6:
(6)
Where ΔHControl and ΔHTreated are the latent heat of fusion
of the control and the Biofield Energy Treated Se,
respectively.
Results and Discussion
Particle Size Analysis (PSA)
The particle size distribution analysis data of
both the control and the Biofield Energy Treated Se
powder samples are presented in Table 1. The particle
size values of the control Se powder at d10, d50, d90, and
D(4,3) were 9.859 µm, 26.595 µm, 53.601 µm, and

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Parameter d10 (µm) d50 (µm) d90 (µm) D(4,3) (µm) SSA (m2/g)
Control 9.859 26.595 53.601 29.513 0.324
Biofield Treated 6.143 22.775 51.29 26.095 0.433
Percent change* (%) -37.69 -14.36 -4.31 -11.58 33.64
Table 1. Particle size distribution of the control and Biofield Energy Treated selenium.
d10, d50, and d90: particle diameter corresponding to 10%, 50%, and 90% of the cumulative distribution,
D(4,3): the average mass-volume diameter, and SSA: the specific surface area. *denotes the
percentage change in the Particle size distribution of the Biofield Energy Treated sample with respect to
the control sample.
Figure 1. PXRD diffractograms of the control and Biofield Energy Treated
selenium powder.

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29.513 µm, respectively. Similarly, the particle sizes of
the Biofield Energy Treated sample at d10, d50, d90, and
D(4,3) were 6.143 µm, 22.775 µm, 51.29 µm, and
26.095 µm, respectively. The particle size values of the
Biofield Energy Treated Se sample were significantly
decreased by 37.69%, 14.36%, 4.31%, and 11.58% at
d10, d50, d90, and D(4,3), respectively compared to the
control sample. Therefore, the specific surface area of
the Biofield Energy Treated sample (0.433 m2/g) was
significantly increased by 33.64% compared with the
control sample (0.324 m2/g). The results suggested that
the Trivedi Effect®-Consciousness Energy Healing
Treatment might act as an external force for breaking
larger particles to smaller one hence increasing the
surface area of Se particles significantly. Pharmaceutical
compounds with smaller particle size increase the
surface area and improve the dissolution rate, and
bioavailability in the body [11, 35]. Therefore, the
Biofield Energy Treated Se powder would offer better
solubility, bioavailability, and therapeutic efficacy
compared to the untreated sample.
Powder X-ray Diffraction (PXRD) Analysis
The diffractogram of the control Se powder
sample showed sharp and intense peaks at Bragg’s
angle (2q) near to 23.32°, 29.64°, 41.06°, 43.53°,
45.17°, 51.69°, and 61.12° (Figure 1). Similarly, the
diffractogram of the Biofield Energy Treated sample
showed sharp and intense peaks at Bragg’s angle (2q)
near to 23.65°, 29.92°, 41.51°, 43.91°, 45.54°, 51.87°,
and 61.57° (Figure 1). The sharp and intense peaks of
both the diffractograms specified that the samples were
crystalline. The highest peak intensity of the control and
Biofield Energy Treated sample were observed at 2θ
equal to 29.64° and 29.92°, respectively (Table 2,
entry 2). The peak intensities of the Biofield Energy
Treated sample were significantly decreased in the
range from 5.23% to 100% compared to the control
sample. However, the crystallite sizes of the Biofield
Energy Treated Se sample were significantly increased
in the range from 75% to 111.7% compared to the
control sample. Overall, the average crystallite size of
the Biofield Energy Treated Se powder (96.94 nm) was
significantly decreased by 7.81% compared to the
control sample (105.16 nm).
As per the literature, the peak intensity of each
diffraction face on the crystalline compound changes
according to the crystal morphology and alterations in
the XRD pattern provide the proof of polymorphic
transitions [36-38]. The Trivedi Effect®-Consciousness
Energy Healing Treatment probably produced the new
polymorphic form of Se through the mediation of
neutrinos [15]. Different polymorphic forms of
pharmaceuticals have significant effects on drug
performance, such as bioavailability, therapeutic
efficacy, and toxicity, because of their physicochemical
properties are different from the original form [39, 40].
Therefore, the Trivedi Effect®-Consciousness Energy
Healing Treated Se would be better in designing novel
pharmaceutical and nutraceutical formulations.
Differential Scanning Calorimetry (DSC) Analysis
The thermal analysis has been performed to
characterize the thermal behavior of the Biofield Energy
Treated Se compared to the control sample (Table 3).
The thermograms of both the control and Biofield
Energy Treated sample showed sharp endothermic
peaks at 222.1°C and 221.5°C, respectively (Figure 2).
The melting point of the Biofield Energy Treated sample
was slightly decreased by 0.27% compared with the
control sample (Table 3).
The latent heat of fusion (∆Hfusion) of the Biofield
Energy Treated sample (78.23 J/g) was significantly
increased by 12.37% compared with the control sample
(69.62 J/g) (Table 3). The significant change in the
∆Hfusion can be attributed to the change in the molecular
chains and the crystal structure of that compound [41].
Thus, it can be assumed that the Biofield Energy
Treatment might be responsible for the improved
thermal stability of the treated Se sample compared to
the control sample.
Conclusion
The Trivedi Effect®-Consciousness Energy
Healing Treatment (Biofield Energy Treatment) showed
significant effects on the crystallite size, particle size,
surface area, and thermal properties of the selenium
powder. The particle size values of the Biofield Energy
Treated sample powder were significantly decreased by
37.69%, 14.36%, 4.31%, and 11.58% at d10, d50, d90,
and D(4,3), respectively. Therefore, the specific surface
area of the Biofield Energy Treatment sample was
significantly increased by 33.64% compared to the

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Figure 2. DSC thermograms of the control and Biofield Energy Treated
selenium sample.

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Entry
No.
Bragg angle (°2q) Peak Intensity (%) Crystallite size (G, nm)
Control Treated Control Treated % change a Control Treated % change b
1 23.32 23.65 514.00 401.00 -21.98 122.90 111.70 -9.11
2 29.64 29.92 1257.00 1056.00 -15.99 154.20 136.90 -11.22
3 41.06 41.51 153.00 145.00 -5.23 81.00 75.00 -7.41
4 43.53 43.91 347.00 313.00 -9.80 107.00 97.00 -9.35
5 45.17 45.54 243.00 214.00 -11.93 81.00 79.00 -2.47
6 51.69 51.87 206.00 140.00 -32.04 87.00 94.00 8.05
7 61.12 61.57 115.00 123.00 -100.00 103.00 85.00 -5.88
8 Average crystallite size 105.16 96.94 -7.81
adenotes the percentage change in the peak intensity of Biofield Energy Treated sample with respect to the
control sample; bdenotes the percentage change in the crystallite size of Biofield Energy Treated sample with
respect to the control sample.
Table 2. PXRD data for the control and Biofield Energy Treated selenium powder.
Sample Melting point (°C) ∆H (J/g)
Control Sample 222.1 69.62
Biofield Energy Treated 221.5 78.23
% Change* -0.27 12.37
Table 3. DSC data for both control and Biofield Energy Treated samples of selenium sample.

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control sample. The PXRD peak intensities of the Biofield
Energy Treatment sample were significantly decreased
in the range from 5.23% to 100% compared with the
control sample. However, the crystallite sizes of the
Biofield Energy Treatment sample were significantly
increased in the range from 75% to 111.7% compared
to the control sample. But, the average crystallite size of
the Biofield Energy Treatment sample was significantly
decreased by 7.81% compared to the control sample.
The melting point of the Biofield Energy Treatment
sample was slightly altered, but the ∆Hfusion significantly
increased by 12.37% compared with the control
sample. The results suggested that the Trivedi
Effect®-Consciousness Energy Healing Treatment might
generate a new polymorphic form of selenium which
would offer better solubility, bioavailability and be
thermally more stable compared with the control
sample. The Biofield Energy Treated selenium would be
more useful to design novel nutraceutical/
pharmaceutical formulations and which might offer
enhanced therapeutic responses against cardiovascular
disease, cancer, muscle disorders, neurological
disorders, type-2 diabetes, viral diseases, stress, aging,
male infertility, degenerative ailments, etc.
Acknowledgements
The authors are grateful to Central Leather
Research Institute, SIPRA Lab. Ltd., Trivedi Science,
Trivedi Global, Inc., Trivedi Testimonials, and Trivedi
Master Wellness for their assistance and support during
this work.
Conflict of Interest
Authors declare no conflict of interest.
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