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The Sea Stars (Echinodermata: Asteroidea): Their Biology, Ecology, Evolution and Utilization OPEN ACCESS

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The Sea stars (Asteroidea: Echinodermata) are comprising of a large and diverse groups of sessile marine invertebrates having seven extant orders such as Brisingida, Forcipulatida, Notomyotida, Paxillosida, Spinulosida, Valvatida and Velatida and two extinct one such as Calliasterellidae and Trichasteropsida. Around 1,500 living species of starfish occur on the seabed in all the world's oceans, from the tropics to subzero polar waters. They are found from the intertidal zone down to abyssal depths, 6,000m below the surface. Starfish typically have a central disc and five arms, though some species have a larger number of arms. The aboral or upper surface may be smooth, granular or spiny, and is covered with overlapping plates. Many species are brightly colored in various shades of red or orange, while others are blue, grey or brown. Starfish have tube feet operated by a hydraulic system and a mouth at the center of the oral or lower surface. They are opportunistic feeders and are mostly predators on benthic invertebrates. They have complex life cycles and can reproduce both sexually and asexually. Most can regenerate damaged parts or lost arms and they can shed arms as a means of defense. The Asteroidea occupy several significant ecological roles. Starfish, such as the ochre sea star (Pisaster ochraceus) and the reef sea star (Stichaster australis), have become widely known as examples of the keystone species concept in ecology. They are sometimes collected as curios, used in design or as logos, and in some cultures, despite possible toxicity, they are eaten. Starfish have been the recent research topic due to their diverse bioactivities, excellent pharmacological properties and complex secondary metabolites including steroids, steroidal glycosides, anthraquinones, alkaloids, phospholipids, peptides, and fatty acids. These chemical constituents exhibit cytotoxic, hemolytic, antiviral, antifungal, and antimicrobial activities and thus have important implications for human health benefits.
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SF Journal of Biotechnology and Biomedical Engineering
2018 | Volume 1 | Edition 2 | Article 1007
ScienceForecast Publications LLC., | https://scienceforecastoa.com/ 1
1
The Sea Stars (Echinodermata: Asteroidea): Their Biology,
Ecology, Evolution and Utilization
OPEN ACCESS
*Correspondence:
Rahman MA, World Fisheries University
Pilot Programme, Pukyong National
University (PKNU), 45 Yongso-ro, Nam-
gu, Busan 48513, Korea.
E-mail: aminur1963@gmail.com;
aminur2017@pknu.ac.kr
Received Date: 27 Jul 2018
Accepted Date: 10 Sep 2018
Published Date: 17 Sep 2018
Citation: Rahman MA, Molla MHR,
Megwalu FO, Asare OE, Tchoundi
A, Shaikh MM, et al. The Sea Stars
(Echinodermata: Asteroidea): Their
Biology, Ecology, Evolution and
Utilization. SF J Biotechnol Biomed
Eng. 2018; 1(2): 1007.
Copyright © 2018 Rahman MA. This is
an open access article distributed under
the Creative Commons Attribution
License, which permits unrestricted
use, distribution, and reproduction in
any medium, provided the original work
is properly cited.
Review Article
Published: 17 Sep, 2018
Abstract
e Sea stars (Asteroidea: Echinodermata) are comprising of a large and diverse groups of sessile
marine invertebrates having seven extant orders such as Brisingida, Forcipulatida, Notomyotida,
Paxillosida, Spinulosida, Valvatida and Velatida and two extinct one such as Calliasterellidae and
Trichasteropsida. Around 1,500 living species of starsh occur on the seabed in all the world's oceans,
from the tropics to subzero polar waters. ey are found from the intertidal zone down to abyssal
depths, 6,000m below the surface. Starsh typically have a central disc and ve arms, though some
species have a larger number of arms. e aboral or upper surface may be smooth, granular or spiny,
and is covered with overlapping plates. Many species are brightly colored in various shades of red or
orange, while others are blue, grey or brown. Starsh have tube feet operated by a hydraulic system
and a mouth at the center of the oral or lower surface. ey are opportunistic feeders and are mostly
predators on benthic invertebrates. ey have complex life cycles and can reproduce both sexually
and asexually. Most can regenerate damaged parts or lost arms and they can shed arms as a means
of defense. e Asteroidea occupy several signicant ecological roles. Starsh, such as the ochre sea
star (Pisaster ochraceus) and the reef sea star (Stichaster australis), have become widely known as
examples of the keystone species concept in ecology. ey are sometimes collected as curios, used in
design or as logos, and in some cultures, despite possible toxicity, they are eaten. Starsh have been
the recent research topic due to their diverse bioactivities, excellent pharmacological properties and
complex secondary metabolites including steroids, steroidal glycosides, anthraquinones, alkaloids,
phospholipids, peptides, and fatty acids. ese chemical constituents exhibit cytotoxic, hemolytic,
antiviral, antifungal, and antimicrobial activities and thus have important implications for human
health benets.
Keywords: Starsh; Biology; Ecology; Evolution; Bioactive compounds
Rahman MA1*, Molla MHR1, Megwalu FO1, Asare OE1, Tchoundi A1, Shaikh MM1 and Jahan B2
1World Fisheries University Pilot Programme, Pukyong National University (PKNU), Nam-gu, Busan, Korea
2Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna, Bangladesh
Introduction
Echinoderms are an entirely marine phylum whose populations are prevalent in benthic
ecosystems throughout the world's oceans. Starsh or sea stars are echinoderms belonging to
the class Asteroidea. ey are a large and diverse class having seven extant orders viz. Brisingida,
Forcipulatida, Notomyotida, Paxillosida, Spinulosida, Valvatida and Velatida and two extint
one such as Calliasterellidae and Trichasteropsida [1,2]. Approximately 1,500 living species of
starsh occur on the seabed in all the world's oceans, from the tropics to subzero polar waters. e
scientic name Asteroidea was given to starsh by the French zoologist de Blainville in 1830. It is
derived from a star and form, likeness, appearance [3]. e class Asteroidea belongs to the phylum
Echinodermata. As well as the starsh, the echinoderms include sea urchins, sand dollars, brittle
and basket stars, sea cucumbers and crinoids.
e larvae of echinoderms have bilateral symmetry, but during metamorphosis, this is replaced
with radial symmetry, typically pentameric [4]. Adult echinoderms are characterized by having a
water vascular system with external tube feet and a calcareous endoskeleton consisting of ossicles
connected by a mesh of collagen bres [5]. Starsh are included in the subphylum Asterozoa, the
characteristics of which include a attened, star-shaped body as adults consisting of a central disc
and multiple radiating arms. e subphylum includes the two classes of Asteroidea, the starsh,
and Ophiuroidea, the brittle stars and basket stars. Asteroids have broad-based arms with skeletal
support provided by calcareous plates in the body wall, while ophiuroids have clearly demarcated
slender arms strengthened by paired fused ossicles forming jointed vertebrate [6].
Most starsh have ve arms that radiate from a central disc, but the number varies with the
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group. It is not unusual in species that typically have ve arms
for some individuals to possess six or more through abnormal
development [7].
Starsh Biology
e body wall consists of a thin cuticle, an epidermis consisting
of a single layer of cells, a thick dermis formed of connective
tissue and a thin coelomic myoepithelial layer, which provides the
longitudinal and circular musculature. e dermis contains an
endoskeleton of calcium carbonate components known as ossicles.
ese are honeycombed structures composed of calcite microcrystals
arranged in a lattice [8]. ey vary in form, with some bearing
external granules, tubercles and spines, but most are tabular plates
that t neatly together in a tessellated manner and form the main
covering of the aboral surface [9]. Some are specialized structures
such as the madreporite (the entrance to the water vascular system),
pedicellariae and paxillae [10]. Pedicellariae are compound ossicles
with forceps-like jaws. ey remove debris from the body surface and
wave around on exible stalks in response to physical or chemical
stimuli while continually making biting movements. ey oen
form clusters surrounding spines [9,10]. Several groups of starsh,
including Valvatida and Forcipulatida, possess pedicellariae [11]. In
Forcipulatida, such as Asterias and Pisaster, they occur in pompom-
like tus at the base of each spine, whereas in the Goniasteridae,
such as Hippasteria phrygiana, the pedicellariae are scattered over
the body surface. Some are thought to assist in defence, while others
aid in feeding or in the removal of organisms attempting to settle on
the starsh's surface [12]. Some species like Novodinia antillensis and
Labidiaster annulatus use their large pedicellariae to capture small
sh and crustaceans [13].
ere may also be papulae, thin-walled protrusions of the
body cavity that reach through the body wall and extend into the
surrounding water. e structures are supported by collagen bres
set at right angles to each other and arranged in a three-dimensional
web with the ossicles and papulae in the interstices. is arrangement
enables both easy exion of the arms by the starsh and the rapid
onset of stiness and rigidity required for actions performed under
stress [14].
Water vascular system
e water vascular system of the starsh is a hydraulic system
made up of a network of uid-lled canals and is concerned with
locomotion, adhesion, food manipulation and gas exchange.
Water enters the system through the madreporite, a porous, oen
conspicuous, sieve-like ossicle on the aboral surface. It is linked
through a stone canal, oen lined with calcareous material, to a ring
canal around the mouth opening. ere are usually two rows of tube
feet but in some species, the lateral canals are alternately long and
short and there appearing to be four rows. e interior of the whole
canal system is lined with cilia [15].
When longitudinal muscles in the ampullae contract, valves in
the lateral canals close and water is forced into the tube feet. ese
extend to contact the substrate. Although the tube feet resemble
suction cups in appearance, the gripping action is a function of
adhesive chemicals rather than suction [16]. Other chemicals and
relaxation of the ampullae allow for release from the substrate. Most
starsh cannot move quickly, a typical speed being that of the leather
star (Dermasterias imbricata), which can manage just 15 cm in a
minute [17]. Some burrowing species from the genera Astropecten
and Luidia have points rather than suckers on their long tube feet and
are capable of much more rapid motion, "gliding" across the ocean
oor. e sand star (Luidia foliolata) can travel at a speed of 2.8 m
per minute [18].
Gas exchange also takes place through other gills known as
papulae, which are thin-walled bulges on the aboral surface of the
disc and arms. Oxygen is transferred from these to the coelomic uid,
which acts as the transport medium for gasses. Oxygen dissolved in
the water is distributed through the body mainly by the uid in the
main body cavity; the circulatory system may also play a minor role
[19].
Digestive system and excretion
e gut of a starsh occupies most of the disc and extends into the
arms. e cardiac stomach is glandular and pouched and is supported
by ligaments attached to ossicles in the arms so it can be pulled back
into position aer it has been everted. e pyloric stomach has two
extensions into each arm: the pyloric caeca. ese are elongated,
branched hollow tubes that are lined by a series of glands, which
secrete digestive enzymes and absorb nutrients from the food. A short
intestine and rectum run from the pyloric stomach to open at a small
anus at the apex of the aboral surface of the disc [20]. e semi-digested
uid is passed into their pyloric stomachs and caeca where digestion
continues and absorption ensues [21]. In more advanced species of
starsh, the cardiac stomach can be everted from the organism's body
to engulf and digest food. When the prey is a clam or other bivalve,
the starsh pulls with its tube feet to separate the two valves slightly,
and inserts a small section of its stomach, which releases enzymes
to digest the prey. e stomach and the partially digested prey are
later retracted into the disc. Here the food is passed on to the pyloric
stomach, which always remains inside the disc [22]. e retraction
and contraction of the cardiac stomach is activated by a neuropeptide
known as NGFFYamide [22]. Some starsh are not pure carnivores,
supplementing their diets with algae or organic detritus. Some of
these species are grazers, but others trap food particles from the water
in sticky mucus strands that are swept towards the mouth along
ciliated grooves [21]. ese cells engulf waste material, and eventually
migrate to the tips of the papulae, where a portion of body wall is
nipped o and ejected into the surrounding water. Some waste may
also be excreted by the pyloric glands and voided with the faces [20].
Starsh do not appear to have any mechanisms for osmoregulation,
and keep their body uids at the same salt concentration as the
surrounding water. Although some species can tolerate relatively
low salinity, the lack of an osmoregulation system probably explains
why starsh are not found in fresh water or even in many estuarine
environments [20].
Sensory and nervous systems
Although starsh do not have many well-dened sense organs,
they are sensitive to touch, light, temperature, orientation and the
status of the water around them. e tube feet, spines and pedicellariae
are sensitive to touch. e tube feet, especially those at the tips of the
rays, are also sensitive to chemicals, enabling the starsh to detect
odour sources such as food [21]. Many starsh also possess individual
photoreceptor cells in other parts of their bodies and respond to light
even when their eyespots are covered. Whether they advance or
retreat depends on the species [23]. While a starsh lacks a centralized
brain, it has a complex nervous system with a nerve ring around the
mouth and a radial nerve running along the ambulacral region of
each arm parallel to the radial canal. e peripheral nerve system
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consists of two nerve nets: a sensory system in the epidermis and a
motor system in the lining of the coelomic cavity. Neurons passing
through the dermis connect the two [23]. e starsh does not have
the capacity to plan its actions. If one arm detects an attractive odour,
it becomes dominant and temporarily over-rides the other arms to
initiate movement towards the prey. e mechanism for this is not
fully understood [23].
Circulatory system
e body cavity contains the circulatory or haemal system. e
vessels form three rings: one around the mouth (the hyponeural
haemal ring), another around the digestive system (the gastric ring)
and the third near the aboral surface (the genital ring). e heart beats
about six times a minute and is at the apex of a vertical channel (the
axial vessel) that connects the three rings. At the base of each arm
are paired gonads; a lateral vessel extends from the genital ring past
the gonads to the tip of the arm. is vessel has a blind end and there
is no continuous circulation of the uid within it. is liquid does
not contain a pigment and has little or no respiratory function but is
probably used to transport nutrients around the body [24].
Secondary metabolites
Starsh produce a large number of secondary metabolites in the
form of lipids, including steroidal derivatives of cholesterol, and fatty
acid amides of sphingosine. e steroids are mostly saponins, known
as asterosaponins, and their sulphated derivatives. ey vary between
species and are typically formed from up to six sugar molecules
(usually glucose and galactose) connected by up to three glycosidic
chains. Long-chain fatty acid amides of sphingosine occur frequently
and some of them have known pharmacological activity. Some are
feeding deterrents used by the starsh to discourage predation.
Others are antifoulants and supplement the pedicellariae to prevent
other organisms from settling on the starsh's aboral surface. Some
are alarm pheromones and escape-eliciting chemicals, the release
of which trigger responses in conspecic starsh but oen produce
escape responses in potential prey [25]. Research into the ecacy
of these compounds for possible pharmacological or industrial use
occurs worldwide [26].
Sexual reproduction
Most species of starsh are gonochorous, there being separate
male and female individuals. Some species are simultaneous
hermaphrodites, producing eggs and sperm at the same time and in a
few of these, the same gonad, called an ovotestis, produces both eggs
and sperm [27]. When these grow large enough they change back into
females [28]. In others, the eggs may be stuck to the undersides of rocks
[29]. In certain species of starsh, the females brood their eggs – either
by simply enveloping them [29] or by holding them in specialized
structures. Brooding may be done in pockets on the starsh's aboral
surface, [30-31] inside the pyloric stomach (Leptasterias tenera) [32]
or even in the interior of the gonads themselves [27]. ose starsh
that brood their eggs by "sitting" on them usually assume a humped
posture with their discs raised o the substrate [33]. Pteraster militaris
broods a few of its young and disperses the remaining eggs, that are
too numerous to t into its pouch [29]. In these brooding species, the
eggs are relatively large, and supplied with yolk, and they generally
develop directly into miniature starsh without an intervening
larval stage [27]. An intragonadal brooder, the young starsh obtain
nutrients by eating other eggs and embryos in the brood pouch [34].
Brooding is especially common in polar and deep-sea species that
live in environments unfavorable for larval development [36] and in
smaller species that produce just a few eggs [40-41].
Spawning takes place at any time of year, each species having
its own characteristic breeding season [37]. e rst individual of a
species to spawn may release a pheromone that serves to attract other
starsh to aggregate and to release their gametes synchronously [38].
In other species, a male and female may come together and form a pair
[39-40]. is behavior is called pseudocopulation [41] and the male
climbs on top, placing his arms between those of the female. When
she releases eggs into the water, he is induced to spawn [38]. Starsh
may use environmental signals to coordinate the time of spawning
(day length to indicate the correct time of the year, [39] dawn or dusk
to indicate the correct time of day), and chemical signals to indicate
their readiness to breed. In some species, mature females produce
chemicals to attract sperm in the sea water [42].
Asexual reproduction
Some species of starsh are able to reproduce asexually as adults
either by ssion of their central discs [48] or by autonomy of one or
more of their arms. Which of these processes occurs depends on the
genus. Among starsh that are able to regenerate their whole body
from a single arm, some can do so even from fragments just 1 cm
(0.4 in) long [49]. Single arms that regenerate a whole individual are
called comet forms. e division of the starsh, either across its disc
or at the base of the arm, is usually accompanied by a weakness in the
structure that provides a fracture zone [50].
e larvae of several species of starsh can reproduce asexually
before they reach maturity [51]. ey do this by autotomizing some
parts of their bodies or by budding [52]. When such a larva senses
that food is plentiful, it takes the path of asexual reproduction rather
than normal development [53]. ough this costs it time and energy
and delays maturity, it allows a single larva to give rise to multiple
adults when the conditions are appropriate [52].
Some species of starsh have the ability to regenerate lost arms
and can regrow an entire new limb given time [49]. A few can regrow
a complete new disc from a single arm, while others need at least part
of the central disc to be attached to the detached part [24]. Regrowth
can take several months or years [49] and starsh are vulnerable to
infections during the early stages aer the loss of an arm. A separated
limb lives o stored nutrients until it regrows a disc and mouth, and
is able to feed again [49]. Other than fragmentation carried out for
the purpose of reproduction, the division of the body may happen
inadvertently due to part being detached by a predator, or part may
be actively shed by the starsh in an escape response [24]. e loss of
parts of the body is achieved by the rapid soening of a special type of
connective tissue in response to nervous signals. is type of tissue is
called catch connective tissue and is found in most echinoderms [54].
An autonomy-promoting factor has been identied which, when
injected into another starsh, causes rapid shedding of arms [55].
Larval development and maturation
Most starsh embryos hatch at the blastula stage. e original
ball of cells develops a lateral pouch, the archenteron. e entrance
to this is known as the blastopore and it will later develop into the
anus. Another invagination of the surface will fuse with the tip of
the archenteron as the mouth while the interior section will become
the gut. At the same time, a band of cilia develops on the exterior.
is enlarges and extends around the surface and eventually onto
two developing arm-like outgrowths. At this stage the larva is known
as a bipinnaria. e cilia are used for locomotion and feeding, their
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rhythmic beat waing phytoplankton towards the mouth [8].
e lifecycle of a starsh varies considerably between species,
generally being longer in larger forms and in those with planktonic
larvae. For example, Leptasterias bexactis broods a small number
of large-yolked eggs. It has an adult weight of 20g, reaches sexual
maturity in two years and lives for about ten years [8]. Pisaster
ochraceus releases a large number of eggs into the sea each year and
has an adult weight of 80g. It reaches maturity in ve years and has a
maximum recorded lifespan of 34 years [8].
Starsh Ecology
Distribution and habitat
Echinoderms, including starsh, maintain a delicate internal
electrolyte balance that is in equilibrium with seawater. is means
that it is only possible for them to live in a marine environment and
they are not found in any freshwater habitats. Starsh species inhabit
all of the world's oceans. Habitats range from tropical coral reefs,
rocky shores, tidal pools, mud, and sand to kelp forests, sea grass and
the deep-sea oor down to at least 6,000m [51]. e greatest diversity
of species occurs in coastal areas, some of which are shown in Figure
1.
Diet preference
Most species of starsh are generalist predators, eating
microalgae, sponges, bivalves, snails and other small animals [52].
e crown-of-thorns starsh consumes coral polyps, [53] while other
species are detritivores, feeding on decomposing organic material
and faecal matter [52,54]. A few are suspension feeders, gathering in
phytoplankton; Henricia and Enhinaster oen occur in association
with sponges, beneting from the water current they produce [55].
Various species have been shown to be able to absorb organic
nutrients from the surrounding water, and this may form a signicant
portion of their diet [55]. e processes of feeding and capture may be
aided by special parts; Pisaster brevispinus, the short-spined pisaster
from the West Coast of America, can use a set of specialized tube feet
to dig itself deep into the so substrate to extract prey [56].
Ecological impact
Starsh are keystone species in their respective marine
communities. eir relatively large sizes, diverse diets and ability to
adapt to dierent environments makes them ecologically important
[57]. Experimental removals of this top predator from a stretch
of shoreline resulted in lower species diversity and the eventual
domination of Mytilus mussels, which were able to outcompete
other organisms for space and resources [58]. Similar results were
found in a 1971 study of Stichasrter australis on the intertidal coast
of the South Island of New Zealand. S. australis was found to have
removed most of a batch of transplanted mussels within two or three
months of their placement, while in an area from which S. australis
had been removed, the mussels increased in number dramatically,
overwhelming the area and threatening biodiversity [59]. e feeding
activity of the omnivorous starsh Oreaster reticulatus on sandy and
seagrass bottoms in the Virgin Islands appears to regulate the diversity,
distribution and abundance of microorganisms. ese starsh engulf
piles of sediment removing the surface lms and algae adhering to
the particles [60]. In addition, foraging by these migratory starsh
creates diverse patches of organic matter, which may play a role in
the distribution and abundance of organisms such as sh, crabs and
sea urchins that feed on the sediment [61]. Starsh sometimes have
negative eects on ecosystems. Outbreaks of crown-of-thorns starsh
have caused damage to coral reefs in Northeast Australia and French
Polynesia [62]. e species has since grown in numbers to the point
where they threaten commercially important bivalve populations.
As such, they are considered pests, and are on the Invasive Species
Specialist Group's list of the world's 100 worst invasive species [63].
Threats
Starsh may be preyed on by conspecics, other starsh
species, tritons, crabs, sh, gulls and sea otters [64,65]. eir rst
lines of defense are the saponins present in their body walls, which
have unpleasant avours [66]. Several species sometimes suer
from a wasting condition caused by bacteria in the genus Vibrio;
[64] however, a more widespread wasting disease, causing mass
mortalities among starsh, appears sporadically. A paper published
in November 2014 revealed the most likely cause of this disease to
be a densovirus the authors named sea star-associated densovirus
(SSaDV) [67]. e protozoan Orchitophrya stellarum is known to
infect the gonads of starsh and damage tissue [64]. Starsh are
vulnerable to high temperatures. Experiments have shown that the
feeding and growth rates of P. ochraceus reduce greatly when their
body temperatures rise above 23°C (73°F) and that they die when their
temperature rises to 30°C (86°F) [68,69]. is species has a unique
Figure 1: Diversity of different types of sea stars in the marine coastal ecosystem.
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ability to absorb seawater to keep itself cool when it is exposed to
sunlight by a receding tide [70]. It also appears to rely on its arms to
absorb heat, so as to protect the central disc and vital organs like the
stomach [71]. Starsh and other echinoderms are sensitive to marine
pollution [72]. e common starsh is considered to be a bioindicator
for marine ecosystems [73]. eir survival is likely due to the nodular
nature of their skeletons, which are able to compensate for a shortage
of carbonate by growing more eshy tissue [74].
Starsh Evolution
Taxonomic diversity and diversity trends
In terms of total number of species, the Asteroidea (n=1890
species) (Table 1) and the Ophiuroidea (n=2064 species) [75] comprise
the two most diverse classes within the living Echinodermata. Species
counts and names utilized are those nominally accepted by the
World Asteroidea Database as valid (or ‘‘accepted’’ by the database).
Following Blake’s classication with modication by Mah and Foltz
[76] the Valvatacea (Valvatida+Paxillosida) includes the greatest
number of species (n = 1224), followed by the Forcipulatacea (n=393
species), the Velatida (n=145 species) and nally the Spinulosida
(Echinasteridae), which includes 135 species (Table 1) [77]. Mah and
Foltz [76] changed the composition of the Valvatacea to include the
Solasteridae, but even with this dierence (n=51 species), from Blake,
prior versions of the Valvatida included more genera and species
than the Paxillosida [78]. Species diversity is disproportionately
distributed among the 36 families of living Asteroidea (Table 1).
Seven families, Ophidiasteridae, Pterasteridae, Echinasteridae,
Asterinidae, Asteriidae, Goniasteridae and Astropectinidae, each
include more than 100 species. e Goniasteridae (n=256) and
the Astropectinidae (n=243) include the largest number of species
within the Asteroidea. Species are not evenly distributed among
genera. Within the Astropectinidae, Astropecten alone includes
43% (104/243) of the total number of species in the family [79]. e
Goniasteridae includes 65 genera, most of which include multiple
species [80]. At least eight goniasterid genera include more than 10
species. Several genera possess disproportionately high numbers of
species relative to other genera within the family. Henricia includes
some 68% (91/133) of the total known species in the Echinasteridae
[77]. Pteraster (n=45) and Hymenaster (n=50) together account for
82% of the total number of species (n=116) in the Pterasteridae [81].
e aforementioned illustrate the extreme cases, but several more
examples of disproportionately high numbers of species/ family
exist. In nearly every instance of a genus with a disproportionately
high numbers of species, these taxa include a global or widely
distributed range. Astropecten is limited largely to tropical and
temperate settings, but Henricia, Pteraster, and Hymenaster all have
cosmopolitan distributions in cold to temperate water settings.
Starsh Bioactives
Starsh imply a special source of polar steroids of an immense
structural diversity, showing a range of biological activities. e
bioactive compounds such as steroids which act as an integral part
of the cell membrane. e steroidal components namely saponins,
asterosaponins, and astropectenol are the major source of compounds
abundantly found in sea stars [82-88]. A basic study of these facts
reveals the search for “Drugs from the Sea” progresses at the rate of
a 10 percent increase in new compounds per year [79]. e isolation
and characterization of bioactive compounds from the sea stars
Superorder Order Family genera species
Forcipulatacea Forcipulatida Asteriidae 35 178
Heliasteridae 2 9
Stichasteridae 9 28
‘‘Pedicellasteridae’’ 7 32
Zoroasteridae 7 36
Total Forcipulatida 60 283
Brisingida Brisingidae 10 63
Freyellidae 7 47
TOTAL Brisingida 17 110
TOTAL Forcipulatacea 77 393
Spinulosida Echinasteridae 8 133
TOTAL Spinulosida 8 133
Valvatacea Poraniidae 7 22
Valvatida Acanthasteridae 1 2
Archasteridae 1 3
‘‘Asterinidae’’ 25 147
Asterodiscididae 4 20
Asteropseidae 5 6
Chaetasteridae 1 4
Ganeriidae 9 21
Goniasteridae 65 256
Leilasteridae 2 4
Mithrodiidae 2 7
Odontasteridae 6 28
Ophidiasteridae 27 106
Oreasteridae 20 74
Podospherasteridae 1 6
Solasteridae 9 51
Caymanostellidae 2 6
TOTAL Valvatida 187 763
Valvatacea Paxillosida Astropectinidae 26 243
Benthopectinidae 8 69
Ctenodiscidae 1 5
Goniopectinidae 3 10
Luidiidae 1 49
Porcellanasteridae 12 30
Radiasteridae 1 5
Pseudarchasteridae 4 29
TOTAL Paxillosida 56 439
TOTAL Valvatacea 243 1224
Velatida Korethrasteridae 3 7
Myxasteridae 3 9
Pterasteridae 8 116
Concentricycloidea Xyloplacidae 1 3
Total Species 343 1890
Table 1: Breakdown of living taxa among the Neoasteroidea from Foltz and Mah
[75,90].
Rahman MA, et al., SF Journal of Biotechnology and Biomedical Engineering
2018 | Volume 1 | Edition 2 | Article 1007
ScienceForecast Publications LLC., | https://scienceforecastoa.com/ 6
in the marine ecosystem is serving a good resource for the human
population to ght against the deadly diseases like cancer.
Elucidation of novel compounds from far eastern sea star
Leptasterias ochotensis illustrated cytotoxic activity towards cancer
cell lines RPMI-7951 and T-47D [82]. Steroidal compounds and
asterosaponins were isolated from cold water star sh Ctenodiscus
crispatus and Culcita novaeguineae, respectively, showing cytotoxicity
against human carcinoma cell lines HepG2 and U87MG ensuing the
apoptosis of the cells, hence playing a signicant role in the anti-
tumor chemotherapy [83,84]. Steroidal compounds were elucidated
from one another species of sea star Astropecten polyacanthus, which
showed cytotoxic activity against the human cancer cell lines HL-60,
PC-3 and SNU-C5 [85]. e crude extracts of the same species of
starsh (A. polyacanthus) also possessed inhibitory eects against the
inammatory components (TNF-α and IL-6) [86]. A polysaccharide
compound extracted from the starsh Asterina pectinifera showed a
chemo-preventive activity against human colon cancer (HT-29) and
human breast cancer by initiating the enzymatic activity which plays a
key task in the carcinogenesis inhibition [87,88]. Few new compounds
of asterosaponins from the starsh Archaster typicus showed cytotoxic
activity against human cervical cancer cell lines and mouse epidermal
cell line [89]. Glycolipids isolated from starsh Narcissia canariensis,
harvested from the coast of Africa showed cytotoxicity against various
adherent human cancerous cell lines (multiple myeloma, colorectal
adenocarcinoma and glioblastoma multiform) [88]. A comparative
study was made between the crude extract from Acanthaster planci
starsh with conventional medicine tamoxifen, a drug used against
the human breast cancer and the extract showed eective apoptotic
activity than the drug tamoxifen in human breast cancer cell line
[90-96]. Hemolytic activity was also studied from various star sh
namely Ophiocoma erinaceus, Acanthaster planci (crown of thorn),
Protoreaster linckii (red knobbed), and Holothuria polii showed
hemolysis when tested against human, chicken, goat and rabbit red
blood cells, hence having a naturally secondary metabolite possessing
hemolytic properties [85-89].
Conclusions
Starsh are some of the unique and most treasured creatures in
the sea, and these fascinating species are the images of the seashore.
ey have profound biological, ecological, cultural, pharmaceutical
and taxonomical signicance. Ecological roles of the starshes in
marine ecosystems are addressed at varied levels. e most prominent
and best-known impact of starshes is due to the common predatory
species in coastal areas. e population dynamics of prey strongly
depends on predation pressure (e.g. the relationship between asteriids
and mussel populations). e rises of falls of the starsh populations
typically depend on the young juvenile recruitment. He uctuation
in settlement success depends on the abundance and health of the
planktonic larvae, and therefore on the phytoplankton enrichment.
Recently starsh have attracted organic chemists, biochemists,
and pharmacologists as a mesmerizing source of marine bioactive
natural products. Numerous secondary metabolites including
steroids, steroidal glycosides, anthraquinones, alkaloids,
phospholipids, peptides, and fatty acids were reported from starsh.
ese biochemical constituents exhibit cytotoxic, hemolytic, antiviral,
antifungal, and antimicrobial, antiaging and antidiabetic activities
and therefore have important implications for the improvement of
general body tone as well as treatment of a number of serious diseases
and health ailments.
Asteroid biodiversity and systematics remains an active area of
research that has brought additional depth to our understanding
of echinoderm evolution and historical changes in the marine
setting. Although much asteroid taxonomy is stable, many new taxa
remain to be discovered with many new species currently awaiting
description. However, until now, the phylogeny of starshes
remains a highly debated topic. Some key issues are central to the
debate, including the monophyly of traditional orders and families
as well as the relationships among the major clades. Morphology-
based and molecular approaches of phylogeny tend to converge,
and future studies should help to minimize the debate. e new
data derived from molecular phylogenetics and the advent of global
biodiversity databases will also present important new springboards
for understanding the global biodiversity and evolution of asteroids.
References
1. Sweet Elizabeth. "Fossil Groups: Modern forms: Asteroids: Extant Orders
of the Asteroidea". University of Bristol. Elizabeth Sweet. 2005.
2. Knott Emily. Asteroidea. Sea stars and starsh. e tree of life project.
2004; 07.
3. "Etymology of the Latin word Asteroidea". My Etymology. 2008.
4. Fox Richard. "Asterias forbesi". Invertebrate Anatomy OnLine. Lander
University. 2007.
5. Wray Gregory A. "Echinodermata: Spiny-skinned animals: sea urchins,
starsh, and their allies". Tree of Life web project. 1999.
6. Stöhr S, O'Hara T. "World Ophiuroidea Database". 2012.
7. Daily Mail Reporter. "You superstar! Fisherman hauls in starsh with eight
legs instead of ve". 1999.
8. Ruppert et al. Vertebrate Zoology: A Functional Evolutionary Approach.
Seventh Edition. 2004: 876.
9. Sweat LH. "Glossary of terms: Phylum Echinodermata". Smithsonian
Institution. 2012.
10. Ruppert et al. A Functional Evolutionary Approach. 2004: 888–889.
11. Carefoot Tom. "Pedicellariae". Sea Stars: Predators & Defenses. A Snail's
Odyssey. 2013.
12. Barnes RSK, Callow P, Olive PJW. e Invertebrates: a new synthesis.
Oxford: Blackwell Scientic Publications. 1988: 158–160.
13. Lawrence JM. "e Asteroid Arm". Starsh: Biology and Ecology of the
Asteroidea. 2013: 15–23.
14. O'Neill P. "Structure and mechanics of starsh body wall". Journal of
Experimental Biology. 1989; 147: 53–89.
15. Ruppert et al. A Functional Evolutionary Approach. 2004: 879–883.
16. Hennebert E, Santos R, Flammang P. "Echinoderms don’t suck: evidence
against the involvement of suction in tube foot attachment" (PDF).
Zoosymposia. 2012; 1: 25–32.
17. Dorit RL, Walker WF, Barnes RD. Saunders College Publishing. Zoology.
1991: 782.
18. Cavey Michael J, Wood Richard L. "Specializations for excitation-
contraction coupling in the podial retractor cells of the starsh Stylasterias
forreri". Cell and Tissue Research. 1981; 218: 475–485.
19. Ruppert et al. A Functional Evolutionary Approach. 2004: 886–887.
20. Ruppert et al. A Functional Evolutionary Approach. 2004 : 885.
21. Carefoot Tom. "Adult feeding". Sea Stars: Feeding, growth & regeneration.
A Snail's Odyssey. 2013.
22. Semmens Dean C, Dane Robyn E, Pancholi Mahesh R, Slade Susan E,
Rahman MA, et al., SF Journal of Biotechnology and Biomedical Engineering
2018 | Volume 1 | Edition 2 | Article 1007
ScienceForecast Publications LLC., | https://scienceforecastoa.com/ 7
Scrivens James H, Elphick Maurice R. "Discovery of a novel neurophysin-
associated neuropeptide that triggers cardiac stomach contraction and
retraction in starsh". Journal of Experimental Biology. 2013. 216: 4047–
4053.
23. Ruppert et al. A Functional Evolutionary Approach. 2004: 883–884.
24. Ruppert et al. A Functional Evolutionary Approach 2004: 886.
25. Lawrence John M, McClintock James B, Amsler Charles D, Baker Bill
J. Chemistry and Ecological Role of Starsh Secondary Metabolites in
"Starsh: Biology and Ecology of the Asteroidea". JHU Press. 2013.
26. Zhang Wen Guo, Yue-Wei Gu, Yucheng. "Secondary metabolites from the
South China Sea invertebrates: chemistry and biological activity". Current
Medicinal Chemistry. 2006; 13: 2041–2090.
27. Byrne Maria. "Viviparity in the sea star Cryptasterina hystera (Asterinidae):
conserved and modied features in reproduction and development".
Biological Bulletin. 2005; 208: 81–91.
28. Ottesen PO, Lucas JS. "Divide or broadcast: interrelation of asexual and
sexual reproduction in a population of the ssiparous hermaphroditic
seastar Nepanthia belcheri (Asteroidea: Asterinidae)". Marine Biology.
1982; 69: 223–233.
29. Crump RG, Emson RH. "e natural history, life history and ecology of
the two British species of Asterina" (PDF). Field Studies. 1983; 5: 867–882.
30. McClary DJ, Mladenov PV. "Reproductive pattern in the brooding and
broadcasting sea star Pteraster militaris". Marine Biology. 1989; 103: 531–
540.
31. Ruppert et al. A Functional Evolutionary Approach 2004: 887–888.
32. Hendler Gordon, Franz David R. "e biology of a brooding seastar,
Leptasterias tenera, in Block Island". Biological Bulletin. 1982; 162: 273–
289.
33. Chia Fu-Shiang. "Brooding behavior of a six-rayed starsh, Leptasterias
hexactis". Biological Bulletin. 1966; 130: 304–315.
34. Byrne M. "Viviparity and intragonadal cannibalism in the diminutive sea
stars Patiriella vivipara and P. parvivipara (family Asterinidae)". Marine
Biology. 1996; 125: 551–567.
35. Gaymer CF, Himmelman JH. "Leptasterias polaris". Starsh: Biology and
Ecology of the Asteroidea. 2013: 182–184.
36. Mercier A, Hamel JF. "Reproduction in Asteroidea". Starsh: Biology and
Ecology of the Asteroidea. 2013: 37.
37. orson Gunnar. "Reproductive and larval ecology of marine bottom
invertebrates". Biological Reviews. 1950; 25: 1–45.
38. Beach DH, Hanscomb NJ, Ormond RFG. "Spawning pheromone in
crown-of-thorns starsh". Nature. 1975; 254: 135–136.
39. Bos AR, GS Gumanao, B Mueller, MM Saceda. "Size at maturation,
sex dierences, and pair density during the mating season of the Indo-
Pacic beach star Archaster typicus (Echinodermata: Asteroidea) in the
Philippines". Invertebrate Reproduction and Development. 2013; 57:
113–119.
40. Run JQ, Chen CP, Chang KH, Chia FS. "Mating behaviour and reproductive
cycle of Archaster typicus (Echinodermata: Asteroidea)". Marine Biology.
1988; 99: 247–253.
41. Keesing John K, Graham Fiona, Irvine Tennille R, Crossing Ryan.
"Synchronous aggregated pseudo-copulation of the sea star Archaster
angulatus Müller & Troschel, 1842 (Echinodermata: Asteroidea) and its
reproductive cycle in south-western Australia". Marine Biology. 2011; 158:
1163–1173.
42. Miller Richard L. "Evidence for the presence of sexual pheromones in free-
spawning starsh". Journal of Experimental Marine Biology and Ecology.
1989; 130: 205–221.
43. Achituv Y, Sher E. "Sexual reproduction and ssion in the sea star Asterina
burtoni from the Mediterranean coast of Israel". Bulletin of Marine
Science. 1991; 48: 670–679.
44. Edmondson CH. "Autotomy and regeneration of Hawaiian starshes"
(PDF). Bishop Museum Occasional Papers. 1935; 11: 3–20.
45. Carnevali Candia, Bonasoro F. "Introduction to the biology of regeneration
in echinoderms". Microscopy Research and Technique. 2001; 55: 365–368.
46. Eaves Alexandra A, Palmer A. Richard. "Reproduction: widespread
cloning in echinoderm larvae". Nature. 2003; 425: 146.
47. Jaeckle William B. "Multiple modes of asexual reproduction by tropical
and subtropical sea star larvae: an unusual adaptation for genet dispersal
and survival". Biological Bulletin. 1994; 186: 62–71.
48. Vickery MS, McClintock JB. "Eects of food concentration and availability
on the incidence of cloning in planktotrophic larvae of the sea star Pisaster
ochraceus". e Biological Bulletin. 2000; 199: 298–304.
49. Hayashi Yutaka, Motokawa Tatsuo. "Eects of ionic environment on
viscosity of catch connective tissue in holothurian body wall" (PDF).
Journal of Experimental Biology. 1986; 125: 71–84.
50. Mladenov Philip V, Igdoura Suleiman, Asotra Satish, Burke Robert D.
Purication and partial characterization of an autotomy-promoting factor
from the sea star Pycnopodia helianthoides (PDF). Biological Bulletin.
1989; 176: 169–175.
51. Mah Christopher, Nizinski Martha, Lundsten Lonny. "Phylogenetic
revision of the Hippasterinae (Goniasteridae; Asteroidea): systematics of
deep sea corallivores, including one new genus and three new species".
Zoological Journal of the Linnean Society. 2010; 160: 266–301.
52. Pearse JS. "Odontaster validus". Starsh: Biology and Ecology of the
Asteroidea. 2013; 124–125.
53. Kayal Mohsen, Vercelloni Julie, Lison de Loma ierry, Bosserelle Pauline,
Chancerelle, Yannick, Georoy Sylvie, et al. Predator crown-of-thorns
starsh (Acanthaster planci) outbreak, mass mortality of corals, and
cascading eects on reef sh and benthic communities. PLoS ONE. 2012;
7: e47363.
54. Turner RL. "Echinaster". Starsh: Biology and Ecology of the Asteroidea.
2012; 206–207.
55. Florkin Marcel. Chemical Zoology V3: Echinnodermata, Nematoda, and
Acanthocephala. Elsevier. 2012: 75–77.
56. Nybakken James W, Bertness Mark D. Marine Biology: An Ecological
Approach. Addison-Wesley Educational Publishers. 1997: 174.
57. Menage BA, Sanford E. "Ecological Role of Sea Stars from Populations to
Meta-ecosystems". Starsh: Biology and Ecology of the Asteroidea. 2013:
67.
58. Paine RT. "Food web complexity and species diversity". American
Naturalist. 1966; 100: 65–75.
59. Paine RT. "A short-term experimental investigation of resource
partitioning in a New Zealand rocky intertidal habitat". Ecology. 1971; 52:
1096–1106.
60. Wullf L. "Sponge-feeding by the Caribbean starsh Oreaster reticulatus".
Marine Biology. 1995; 123: 313–325.
61. Scheibling RE. "Dynamics and feeding activity of high-density aggregations
of Oreaster reticulatus (Echinodermata: Asteroidea) in a sand patch
habitat". Marine Ecology Progress Series. 1980; 2: 321–327.
62. Brodie J, Fabricius K, De'ath G, Okaji K. "Are increased nutrient inputs
responsible for more outbreaks of crown-of-thorns starsh? An appraisal
of the evidence". Marine Pollution Bulletin. 2005; 51: 266–278.
63. Byrne M, O'Hara TD, Lawrence JM. "Asterias amurensis". Starsh: Biology
and Ecology of the Asteroidea. 2013: 177–179.
Rahman MA, et al., SF Journal of Biotechnology and Biomedical Engineering
2018 | Volume 1 | Edition 2 | Article 1007
ScienceForecast Publications LLC., | https://scienceforecastoa.com/ 8
64. Robles C. "Pisaster ochraceus". Starsh: Biology and Ecology of the
Asteroidea. 2013: 166–167.
65. Scheibling RE. "Oreaster reticulatus". Starsh: Biology and Ecology of the
Asteroidea. 2013; 150.
66. Andersson L, Bohlin L, Iorizzi M, Riccio R, Minale L, Moreno-López W,
et al. Biological activity of saponins and saponin-like compounds from
starsh and brittle-stars. Toxicon. 1989; 27: 179–188.
67. Hewson Ian, Button Jason B, Gudenkauf Brent M, Miner Benjamin,
Newton Alisa L, Gaydos Joseph K, et al. Densovirus associated with sea-
star wasting disease and mass mortality. 2014; 111: 17278–17283.
68. Peters LE, Mouchka ME, Milston-Clements RH, Momoda TS, Menge BA.
"Eects of environmental stress on intertidal mussels and their sea star
predators". Oecologia. 2008; 156: 671–680.
69. Pincebourde S, Sanford E, Helmuth B. Body temperature during low tide
alters the feeding performance of a top intertidal predator". Limnology and
Oceanography. 2008; 53: 1562–1573.
70. Pincebourde S, Sanford E, Helmuth B. "An intertidal sea star adjusts
thermal inertia to avoid extreme body temperatures". e American
Naturalist. 2009; 174: 890–897.
71. Pincebourde S, Sanford E, Helmuth B. "Survival and arm abscission
are linked to regional heterothermy in an intertidal sea star". Journal of
Experimental Biology. 2013; 216: 2183–2191.
72. Newton LC, McKenzie JD. "Echinoderms and oil pollution: A potential
stress assay using bacterial symbionts". Marine Pollution Bulletin. 1995;
31: 453–456.
73. Temara A, Skei JM, Gillan D, Warnau M, Jangoux M, Dubois Ph. "Validation
of the asteroid Asterias rubens (Echinodermata) as a bioindicator of spatial
and temporal trends of Pb, Cd, and Zn contamination in the eld". Marine
Environmental Research. 1998; 45: 341–356.
74. Gooding Rebecca A, Harley Christopher DG, Tang Emily. Elevated water
temperature and carbon dioxide concentration increase the growth of a
keystone echinoderm. Proceedings of the National Academy of Sciences.
2009; 106: 9316–9321.
75. Malyarenko TV, Kicha AA, Ivanchina NV, Kalinovsky AI, Popov RS, et al.
Asterosaponins from the Far Eastern starsh Leptasterias ochotensis and
their anticancer activity. Steroids. 2014; 87: 119-127.
76. Sto¨hr S, O’Hara T. World Ophiuroidea Database. 2007.
77. Mah CL, Foltz DW. Molecular Phylogeny of the Valvatacea (Asteroidea,
Echinodermata). Zool J Linn Soc. 2011; 161: 769–788.
78. Mah C, Hansson H. Echinasteridae. In: Mah CL. World Asteroidea
Database. Accessed through: Mah CL. World Asteroidea Database. 2011.
79. Blake DB. Some biological controls on the distribution of shallow-water
sea stars (Asteroidea; Echinodermata). Bull Mar Sci. 1983; 33: 703–712.
80. Mah C, Hansson H. Astropectinidae. In: Mah CL. World Asteroidea
Database. Accessed through: Mah CL. 2011. World Asteroidea Database.
81. Mah C, Hansson H. Goniasteridae. In: Mah CL. World Asteroidea
Database. Accessed through: Mah CL. 2011.
82. Tran HQ, Lee D, Han S, Kim C, Yim JH, et al. Steroids from the Cold
Water Starsh Ctenodiscus crispatus with Cytotoxic and Apoptotic
Eects on Human Hepatocellular Carcinoma and Glioblastoma. Cells Bull
Korean Chem Soc. 2014.
83. Guang C, Xiang Z, Hai FT, Yun Z, Xin H, et al. Asterosaponin 1, a cytostatic
compound from the starsh Culcita novaeguineae, functions by inducing
apoptosis in human glioblastoma U87MG cells. Journal of Neuroncology.
2006; 79: 235-241.
84. Faulkner DJ. Chemical Riches from the Ocean. Chem Brit. 1995; 680-684.
85. Nguyen P, Nguyen X, Bui T, Nguyen H, Pham V, Nguyen V, et al. Steroidal
Constituents from the Starsh Astropecten polyacanthus and their
Anticancer Eects Chem. Pharm Bull. 2013; 61: 1044-1051.
86. Nguyen P, Nguyen X, Bui T, Tran H, Tran T, et al. Anti- Inammatory
Components of the Starsh Astropecten polyacanthus. 2012; 11: 2917-
2926.
87. Kyung-Soo N, Yun H. Chemopreventive eects of polysaccharides extract
from Asterina pectinifera on HT-29 human colon adenocarcinoma cells.
BMB reports. 2008; 42: 277-280.
88. Kicha AA, Ivanchina NV, Huong TT, Kalinovsky AI, Dmitrenok PS, et al.
Two new asterosaponins, archasterosides A and B, from the Vietnamese
starsh Archaster typicus and their anticancer properties. Bioorg Med
Chem Lett. 2013; 20: 3826-3830.
89. Fereshteh F, Gaetane W, Monique C, Jean-Michel K, Gilles B. Cytotoxicity
on Human Cancer Cells of Ophidiacerebrosides Isolated from the African
Starsh Narcissia canariensis. Mar Drugs. 2010; 8: 2988-2998.
90. Ahmed F, Salizawati M, Farid C, Faisal M, Chung P, et al. Apoptosis
induced in human breast cancer cell line by Acanthaster planci starsh
extract compared to Tamoxifen. African Journal of Pharmacy and
Pharmacology. 2012; 6: 129-134.
91. Elaheh A, Mohammad N, Javad B, Kazem P, Javad As. Hemolytic and
cytotoxic eects of saponin like compounds isolated from Persian Gulf
brittle star (Ophiocoma erinaceus). Journal of Coastal Life Medicine. 2014;
2: 762-768.
92. Chiu Le, Wann S, Hernyi Justin H, Fwu H. Hemolytic activity of venom
from crown-of-thorns starsh Acanthaster planci spines. Journal of
Venomous Animals and Toxins including Tropical Diseases. 2013; 9: 22.
93. Suguna A, Bragadeeswaran S, Priyatharsini S, Mohanraj M,
Sivaramakrishnan S. Cytolytic and antinociceptive activities of starsh
Protoreaster linckii (Blainvilli, 1893). African Journal of Pharmacy and
Pharmacology. 2013; 7: 2734-2742.
94. Canicattì C, Parrinello N, Arizza V. Inhibitory activity of sphingomyelin on
hemolytic activity of coelomic uid of Holothuria polii (Echinodermata).
Developmental and comparative immunology. 1987; 11: 29-35.
95. R Sumithaa R, Banu N, Deepa, Parvathi V. Novel Natural Products
from Marine Sea Stars. Current Trends in Biomedical Engineering and
Biosciences. 2017; 2: CTBEB.MS.ID.555592.
96. Mah CL, Blake DB. Global Diversity and Phylogeny of the Asteroidea
(Echinodermata). PLoS ONE. 2012; 7: e35644.
ResearchGate has not been able to resolve any citations for this publication.
Article
Full-text available
Objective: To isolate and characterize the saponin from Persian Gulf brittle star (Ophiocoma erinaceus) and to evaluate its hemolytic and cytotoxic potential. Methods: In an attempt to prepare saponin from brittle star, collected samples were minced and extracted with ethanol, dichloromethane, n-buthanol. Then, concentrated n-butanol extract were loaded on HP-20 resin and washed with dionized water, 80% ethanol and 100% ethanol respectively. Subsequently, detection of saponin was performed by foaming property, fourier transform infrared spectroscopy and hemolytic analysis on thin layer chromatography. The cytotoxic activity on HeLa cells was evaluated through 3-[4,5-dimethylthiazol-2-yl]-2,5- diphenyltetrazoliumbromide (MTT) assay and under invert microscopy. Results: The existence of saponin in Ophiocoma erinaceus were approved by phytochemical method. The presence of C-H bond, C-O-C and OH in fourier transform infrared spectrum of fraction 80% ethanol is characteristic feature in the many of saponin compounds. Hemolytic assay revealed HD 50 value was 500 µg/mL. MTT assay exhibited that saponin extracted in IC50 value of 25 µg/mL inducsd potent cytotoxic activity against HeLa cells in 24 h and 12.5 µg/mL in 48 h, meanwhile in lower concentration did not have considerable effect against HeLa cells. Conclusions: These findings showed that only 80% ethanol fraction Persian Gulf brittle star contained saponin like compounds with hemolytic activity which can be detected simply by phytochemical that can be appreciable for future anticancer research.
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