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Low level light therapy stimulates wound healing in a diabetic mouse model in a wavelength dependent manner

Authors:

Abstract

Low level light therapy (LLLT) has been shown to positively modulate wound healing processes. So far, most studies are being performed with lasers in the red to near-infrared spectra. Recently, we showed that also shorter wavelengths can significantly influence biological systems such as nitric oxide (NO) metabolism. Therefore, the aim of this study was to evaluate and compare the therapeutic effects of pulsed REPULS-LED light at different wavelengths on wound healing in a diabetic mouse wound healing model.
European Cells and Materials Vol. 33 Suppl. 2, 2017 (P561) ISSN 1473-2262
http://www.ecmjournal.org
P561 Low level light therapy stimulates wound healing in a diabetic mouse model in a
wavelength dependent manner
Paul Slezak1,2, Claudia Keibl1,2, Sanja Sutalo1,2, Cyrill Slezak3, Stefan Rieger1,2, Johannes
Hackethal1, Heinz Redl1,2, Peter Dungel1,2
1Ludwig Boltzmann Institute for experimental and clinical Traumatology, Vienna, Austria,
2Austrian Cluster for Tissue Regeneration, Vienna, Austria, 3Utah Valley University, Orem/UT,
USA
Low level light therapy (LLLT) has been shown to positively modulate wound healing processes.
So far, most studies are being performed with lasers in the red to near-infrared spectra. Recently,
we showed that also shorter wavelengths can significantly influence biological systems such as
nitric oxide (NO) metabolism. Therefore, the aim of this study was to evaluate and compare the
therapeutic effects of pulsed REPULS-LED light at different wavelengths on wound healing in a
diabetic mouse wound healing model.
A dorsal excision wound was created at the back of diabetic (db/db) mice and was monitored over
a period of 28 days. Every second day the wound was subjected to LED illumination (REPULS)
with a wavelength of either 470 nm (blue), 540 nm (green) or 629 nm (red), each at 50mW/cm2
and compared to a non-treated control group. Wound size and wound perfusion was assessed
and correlated to wound temperature and light absorption of different wavelengths in the tissue.
Red and green light therapy positively stimulated wound healing. Red light led to a significant
higher reduction in wound size at day 12 post surgery while green light showed a strong trend.
Accordingly, the wound healing rate up to day 12 was 60% higher in both the red and the green
light treated group. Blue light was ineffective in this setting. Light absorption was wavelength-
dependent and was associated with significantly increased wound perfusion as measured by
laser Doppler imaging in the red light treated group. Shorter wavelengths ranging from green to
blue significantly increased wound surface temperature, while red light, which penetrates deeper
into tissue, led to a significant increase body core temperature.
In summary, wound treatment with pulsed red or green light resulted in improved wound healing
in diabetic mice. Since impeded wound healing in diabetic patients poses a severe, ever-
increasing socio-economic problem, LED therapy may be a cost-effective and easily applied
supportive treatment for diabetic wound therapy.
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