10.02 - Tuberculosis
Determination of CD4, CD8 and IL-8 levels in
serum and BAL fluid of patients with pulmonary
Chronic diseases, Infections, Tuberculosis
A. Abedini1, M. Ramazanpour1, E. Mortaz2, H. Jamaati1, K. Taghavi1, F. Razavi1, A. Kiani3
1Chronic Respiratory Diseases Research Center, National Research Institute of Tuberculosis and
Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences - tehran (Iran), 2Division
of Pharmacology and Pathophysiology, Utrecht Institute for Pharmaceutical Sciences, Faculty of
Sciences, Utrecht University, Utrecht, The Netherlands; Clinical Tuberculosis and Epidemiology
Research Center, National Research Institute for Tuberculosis and Lung Diseases (NRITLD), Shahid
Beheshti University of Medical Sciences - tehran (Iran), 3Tracheal Diseases Research Center,
National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti
University of Medical Sciences - tehran (Iran)
Background: Anthracosis is the black pigmentation of the bronchial mucosa, caused by the deposition of
carbon particles, silica, quartz, etc., in the mucosa, sub mucosa, and inside the macrophages, and in some
cases, associated with pulmonary tuberculosis (TB).
Aims and Objectives: The aim of the present study is to compare the serum and BAL fluid levels of
CD4/CD8 ratio and IL-8, as potential diagnosis and prognosis biomarkers or anthracosis.
Methods: 30 anthracosis patients, were included in the study. BAL sample and blood samples were
collected, and sent to the Immunology laboratory for analysis. Patients with no anthracosis on
bronchoscopy, and suspected to have TB, were included in the control group. In addition, BAL samples
were used for BK-PCR.
Results: 30 cases and 30 controls were included in the study. There were no significant differences in IL-8
of patients with anthracosis, compared to the control group. There were significant differences of CD4/CD8
ratio in BAL fluid, between anthracosis patients and the control group (1.01±0.77 vs. 2.41±3.50; p= 0.04),
and significant differences were observed in serum level between two groups (p=0.02). Pulmonary TB was
confirmed in 88.9% of patients with anthracosis, which was significantly higher than the control group.
Conclusions: These results suggest that changes in CD4/C8 levels in serum and BAL fluid may play an
important role in the diagnosis or prognosis of anthracosis. In addition, due to the strong association of
anthracosis and pulmonary tuberculosis, TB should be considered in patients with anthracosis, which in
turn can lead to the early diagnosis and treatment of the patients.