The subject of this paper is the relationship between innovative performance and capabilities of firms that, in the search for external knowledge to innovate, interact with scientific and research institutions. The innovation capability is understanding as major capability formed by development, operation, management and transaction capability (ZAWISLAK et al., 2012, 2013). Considering this, the aim of this paper is to understand the behavior of innovation capabilities and performance of firms that interact and the ones that do not interact with scientific and research institutions. For this, it was used data from a project survey (“Paths of Innovation in Brazilian Industry”) that aimed at deepening the understanding of Brazilian industries’ innovation dynamics. Data analysis for this study comprised 1,331 valid questionnaires. The interacting firms cluster, which encompasses firms that interact with scientific and research institutions, consists of 370 firms, and the non‐interacting firms cluster, which has firms that do not interact, presents 949 firms. Results show that there are differences between innovation capabilities of firms that interact compared to those that do not interact with scientific and research institutions. Firms that establish interactions have more development, operations, management and transaction capabilities. Therefore, a significant difference between the two groups of firms relates with the development capability. This finding corroborates what had already been described in the literature, that the interaction is one of the mechanisms whereby firms can improve their development capability by developing new products and new technologies. Interacting firms also have higher innovative performance, measured by firm revenue, profit and market share growth. Thus, it is noted that interaction with scientific and research institutions does not only improve firms’ development capability, but it also makes them obtain the innovative profit.