In interactions, the culture of the participants influences their contributions and interpretations. Stand-up comedians articulate contemporary culture by making mutually manifest cultural beliefs and representations within the performance space, and teach the audience how to use them. This paper investigated how Nigerian stand-up comedians employ cultural assumptions and representations in their performances. Using relevance theory for analysis and seven routines from seven Nigerian stand-up comedians as the data, this study explored how Nigerian stand-up comedians bring shared cultural knowledge into their performances. Nigerian stand-up comedians joke with culture by manipulating shared cultural representations, distorting collective knowledge, manipulating stereotypes and projecting personal beliefs. By joking with cultural beliefs and representations within the performance space, Nigerian stand-up comedians mediate and negotiate what “contemporary culture” should be.