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Table S1.- Primers used in this study.
Primer name Sequence Description/Purpose
WlpxO1
AAGGACAGTGACGTTATGTTCGCAGCAGT
GCAGGCTGGAGCTGCTTC
Construction of ∆lpxO mutant
WlpxO2
TACCGCCGGCCGGCTTCAGAGGAGGCTCA
TATGAATATCCTCCTTAG
Construction of ∆lpxO mutant
lpxOF TTTCATCTCCATCATGCCAA Confirmation of ∆lpxO mutant
lpxOR CGGATGGATAGACTCAAGGC Confirmation of ∆lpxO mutant
Wfnr1
CAATTACGGCTTGAGCAGACCTATGATCC
CGTGTAGGCTGGAGCTGCTTC
Construction of ∆fnr mutant
Wfnr2
GCTGTTTTCAATAGTAATATACTTACCTTT
CATATGAATATCCTCCTTAG
Construction of ∆fnr mutant
fnrF CGCCATGAAGGCTATCTTTTATTAT Confirmation of ∆fnr mutant
fnrR CAGAAGATAACATCAATGGTTTAGC Confirmation of ∆fnr mutant
lac GACCATTTTCAATCCGCA Confirmation of pKG136 single-copy integration
kan TTTCTAGAGCTGTTAAAAGGACA Confirmation of pKG136 single-copy integration
qlpxOF ACGGCATTTCCAGAGTCGCA Quantification of lpxO transcript levels using qRT-PCR
qlpxOR GGCATAGGGGTCGCGATGTT Quantification of lpxO transcript levels using qRT-PCR
qrpoDF TGAAGTGTTCAAACAGTTCCGTCT Quantification of rpoD transcript levels using qRT-PCR
qrpoDR AGACCGGTCTCTTCTTCAATCTGC Quantification of rpoD transcript levels using qRT-PCR
arcAF TGCAAATTTGTGATGAAAGC
Cloning of S. Enteritidis arcA (promoter included) in
pBAD-TOPO
arcAR AAAGAGTACGTCATAACGGC
Cloning of S. Enteritidis arcA (promoter included) in
pBAD-TOPO
arcA_PF TAAGAGGAATAATAAATGCAGACCCCGC Cloning of S. Enteritidis arcA ORF in pBAD-TOPO
arcA_PR ATCCTGCAGGTCGCCGCAGA Cloning of S. Enteritidis arcA ORF in pBAD-TOPO
fnr_PF TAAGAGGAATAATAAATGATCCCGGAAA Cloning of E. coli K12 fnrD154A ORF in pBAD-TOPO
fnr_PR AGCGACGTTGCGGGTATGAC Cloning of E. coli K12 fnrD154A ORF in pBAD-TOPO
pBADF ATGCCATAGCATTTTTATCC Confirmation of PCR product cloned in pBAD-TOPO
pBADR GATTTAATCTGTATCAGG Confirmation of PCR product cloned in pBAD-TOPO
lpxO_up1F* AGTTTCATCTCCATCATGCC Amplification of the lpxO promoter region including ABS-1
lpxO_up1R TGGCCCTTTTAAGTTGTAGA Amplification of the lpxO promoter region including ABS-1
lpxO_up2F AAAGCATTGTGATCTGGCAG Amplification of the lpxO promoter region including ABS-2
lpxO_up2R CAACTTAAAAGGGCCATTTT Amplification of the lpxO promoter region including ABS-2
lpxO_up3F GTAACCTTCGCGAAGATAAA Amplification of the lpxO promoter region including ABS-3
lpxO_up3R* TCTAAGAAAACCTCATCCCG Amplification of the lpxO promoter region including ABS-3
SP6 TACGATTTAGGTGACACTATAG Amplification of pGEM-T Easy polylinker region
T7 TAATACGACTCACTATAGGG Amplification of pGEM-T Easy polylinker region
* Primers lpxO_up1F and lpxO_up3R were also used to amplify the complete promoter region of lpxO, which includes ABS-1, ABS-2
and ABS-3.
Underlined sequences correspond to the region that anneals to the 5’ or 3’ end of the antibiotic-resistance cassette in template vectors
pCLF3 and pCLF4.