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Beneficial effects of dietary silver nanoparticles and silver nitrate on broiler nutrition

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This study was conducted to investigate the growth modulatory effects of dietary supplementation with silver nanoparticles (Ag NPs) and Ag nitrate on broiler chickens. Thirty 15-day-old chicks were equally divided into the control group (fed basal diets), the Ag-nano group (fed basal diets supplemented with 50 ppm/kg of Ag NPs for 12 d), and the Ag nitrate group (fed basal diets supplemented with 100 ppm/kg Ag nitrate for 12 days). Chicks fed Ag NPs showed increased body weight gain and muscle weight, improved feed efficiency, and increased ash digestibility, while Ag digestibility tend to increase but not significant. Plasma triiodothyronine contents, and muscle Ag and nitrogen contents as well as a significant increase in the following mRNA levels in muscle tissue: insulin-like growth factor-1 (IGF1), glucose transporters (Glut1, Glut3), citrate synthase (CS), and glutathione peroxidase (GPx), whereas the atrogin-1, fatty acid synthase (FAS), acetyl CoA carboxylase (ACC), lactate dehydrogenase (LDH), and carnitine palmitoyl transferase 1A (CPT1A) mRNA levels were unchanged. However, these chicks exhibited decreased levels of plasma cholesterol, triglyceride, and glucose. Dietary supplementation with Ag NPs improved the growth performance of broiler chickens.
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RESEARCH ARTICLE
Beneficial effects of dietary silver nanoparticles and silver nitrate
on broiler nutrition
Ahmed A. Saleh
1
&Mohammed A. El-Magd
2
Received: 22 March 2018 /Accepted: 5 July 2018
#Springer-Verlag GmbH Germany, part of Springer Nature 2018
Abstract
This study was conducted to investigate the growth modulatory effects of dietary supplementation with silver nanoparticles (Ag
NPs) and Ag nitrate on broiler chickens. Thirty 15-day-old chicks were equally divided into the control group (fed basal diets),
the Ag-nano group (fed basal diets supplemented with 50 ppm/kg of Ag NPs for 12 d), and the Ag nitrate group (fed basal diets
supplemented with 100 ppm/kg Ag nitrate for 12 days). Chicks fed Ag NPs showed increased body weight gain and muscle
weight, improved feed efficiency, and increased ash digestibility, while Ag digestibility tend to increase but not significant.
Plasma triiodothyronine contents, and muscle Ag and nitrogen contents as well as a significant increase in the following mRNA
levels in muscle tissue: insulin-like growth factor-1 (IGF1), glucose transporters (Glut1, Glut3), citrate synthase (CS), and
glutathione peroxidase (GPx), whereas the atrogin-1, fatty acid synthase (FAS), acetyl CoA carboxylase (ACC), lactate dehy-
drogenase (LDH), and carnitine palmitoyl transferase 1A (CPT1A) mRNA levels were unchanged. However, these chicks
exhibited decreased levels of plasma cholesterol, triglyceride, and glucose. Dietary supplementation with Ag NPs improved
the growth performance of broiler chickens.
Keywords Silver nanoparticles .Silver nitrate .Broiler .Growth .Gene expression
Introduction
Silver (Ag) has been valued throughout history because many
of its properties are useful to humans and animals. Ag-
containing compounds such as Ag nitrate and Ag oxide vary
in solubility from readily water soluble to barely water solu-
ble. Ag nanoparticles (Ag NPs) are fine particles of metallic
Ag that have at least one dimension smaller than 100 nm. One
of the most beneficial uses of Ag NPs is as a potent antimi-
crobial agent because they are toxic to fungi, bacteria, viruses,
and algae (Cho et al. 2005; Percival et al. 2007). Therefore, Ag
NPs are widely used in food storage, household products,
disinfectants, textiles, and medical equipment (Furno et al.
2004; Poynton et al. 2012). Several hypotheses have de-
scribed how the use of Ag NPs became established in the
poultry industry: (1) The antibacterial properties of Ag NPs
affect microbial populations without inducing resistance and
increase the animalsanabolic activity, which stimulates their
growth and development [1]; (2) Ag NPs contribute to the
oxygen demand and increase the rate of metabolism, which
further improve growth (Loghman et al. 2012); (3) Ag NPs
affect the gene expression of fibroblast growth factor (FGF),
which stimulates the proliferation and differentiation of blood
vessels, muscles, and fibroblasts cells; promotes angiogenesis
via increases in vascular endothelial growth factor (VEGF),
which is necessary for the proliferation and organization of
endothelial cells; increases levels of paired box (Pax7) tran-
scription factor; promotes the development of satellite cells in
muscles; and increases levels of proliferating cell nuclear an-
tigen (PCNA) and trimmer of DNA synthesis and repair; and
these factors are essential during embryogenesis and are im-
portant for post-natal development (Sawosz et al. 2009); and
(4) Ag NPs increase the cells immunity by stimulating heat
shock protein (HSP) synthesis without activating the pro-
inflammatory pathway (Atiyeh et al. 2007). Because molecule
bioavailability increases when they are degraded into small
Responsible editor: Philippe Garrigues
*Ahmed A. Saleh
ahmed.saleh1@agr.kfs.edu.eg
1
Department of Poultry Production, Faculty of Agriculture,
Kafrelsheikh University, 333516, Kafrelsheikh, Egypt
2
Anatomy Departments, Faculty of Veterinary Medicine,
Kafrelsheikh University, Kafrelsheikh, Egypt
Environmental Science and Pollution Research
https://doi.org/10.1007/s11356-018-2730-7
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