ThesisPDF Available

Optimization of micropropagation of Haworthia (Haworthia truncata)

Authors:
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



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M.Sc

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
Haworthia truncata
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

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

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

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
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
  
Haworthia truncate
MS½MS BAP
KinMS½MS 
NAAIBA
MS½MS

Kin
BAP
BAPKin
½MS IBA


Abstract
In the present study, the effects of media and cytokinins on multiplication and
auxins on rooting of Haworthia truncate have been studied. The leaf explants
containing the bud were cultured to MS and ½MS containing 0, 1 and 2 mg/l
BAP or 1 and 2 mg/l Kin for proliferation and MS and ½MS supplemented
with 0, 1 and 2 mg/l NAA or 1 and 2 mg/l IBA for rooting. The results
showed that there was no significant difference between the two basic media
except in the fresh weight of the shoot (MS, 2.40 g and ½MS, 2. 21 g). The
highest shoot proliferation (%) was obtained at 2 and 1 mg/l Kin (87.7 and
78.9%, respectively), and the highest average shoot number per explant was
found in 2 and 1 mg/l BAP (4.6 and 4.4, respectively). Also, the maximum
shoot fresh weight (2.62 g) and dry weight (0.91 g) were observed in 2 mg/l
BAP and 2 mg/l Kin, respectively. The highest rooting percentage was
obtained in MS medium supplemented with 1 and 2 mg/l IBA (90.5 and
80.5%, respectively) and the highest root number was acheived in the media
containing auxin.
Keywords: Haworthia, media, growth regulators, proliferation, rooting
Islamic Azad University
Karaj Branch
Faculty of Agriculture and Natural Resources
Department of Horticultural Science
Thesis submitted for the MSc in Horticultural Science
Physiology and Breeding of Flower and Ornamental Plants
Subject
Optimization of micropropagation of Haworthia (Haworthia truncata)
Supervisor:
Dr. Seied Mehdi Miri
Consulting Advisor:
Dr. Mohammad Farjadi Shakib
By:
Mahdi Hashemi
Summer 2018
... The commercial production of ornamental plants is growing worldwide. Recent modern techniques of propagation have been developed which could help growers to meet the demand of the horticultural industry (17,33,40,41). In vitro culture method has been practiced as a technique to various types of ornamental plants for large-scale plant multiplication of elite superior varieties (6,33). ...
Conference Paper
Full-text available
Ornamental industry has applied immensely in vitro propagation approach for large-scale plant multiplication at very high rates of elite superior varieties. Tissue culture of ornamental plants is affected by many factors such as plant genotype, the kind of explant and the physical environment (light, temperature, humidity and CO2) in addition to the medium composition and growth regulators.
... Plant tissue culture is a technique used for producing plantlets and implies the culture of aseptic small sections of tissues and organs in vessels with defined culture medium and under controlled environmental conditions (3,17). This technique is suitable for rapid mass propagation of elite genotypes and the production of virus free plants as well as independent from seasonal influences (15,16). Also, the establishment of an efficient callus induction protocol is an important step for a successful ornamental breeding through somaclonal variation and transformation. ...
Conference Paper
Full-text available
Ornamental plants are produced primarily for their artistic value, thus the propagation and improvement of quality attributes and the creation of novel variation are important economic goals for floriculturists. Micropropagation is an alternative mean of propagation that can be employed in mass multiplication of plants in relatively shorter time. Recent modern techniques of propagation have been developed which could facilitate large scale production of true-to-type plants. Successful in vitro propagation of ornamental plants is now being used for commercialization. Factors such as medium, plant growth regulators, carbohydrate and gelling agent which substantially affected on the success of plant tissue culture have been reviewed.
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