Antimicrobial, Antioxidant and Antihypertensive Activities of Major Bioactive Compounds from The Stems of Crossostephium chinense L.

Abstract

Crossostephium chinense L. or Hai-Fu-Rung as a new source of herbal medicine was naturally grows near coastal areas on limestone and coral formations in Taiwan and South east of Asia’s region. This plant is one of the famous TCM (Traditional China Medicine) that traditionally used for treating colds, measles, rheumatic, and bronchitis. Extract of whole plant of C. chinense have been reported to exhibit diverse biological activities such as anti-proliferative, hepatoprotective, and anti-inflammation with ameliorative effect, but only a few of these studies involved as antimicrobial, antioxidant, and antihypertensive agent especially from stem part. Interestingly, even C. chinense is a famous herbal medicine, but it still rare, threatened in the wild, and need further investigation especially for its stem part. Hence, this study was conducted to investigate the major of this plant. First result of this study was obtained nine major compounds from crude methanol extract of this plant and identified as: lupeol, b-sitostenone, octadecyl (E)-p-coumarate, cycloeucalenol, oleanolic acid, ursolic acid, stigmastan-3-one, betulin, and ergone. Furthermore, some biological assays such as antimicrobial, antioxidant and antihypertensive activities were evaluated by inhibitory assay; DPPH, FTC, and TBA assays; and ACE inhibitory assay, respectively. Rat aorta ring assay also could be used for completely understanding of antihypertensive effect from air dried stems of C. chinense.