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Anti-oxidative Fraction of Lycorma delicatula Alleviates Inflammatory Indicators

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Abstract

Lycorma delicatula is a reluctant pest capable of living in the stems of several tree species. The population has recently expanded in Korea due to several environmental factors including climate change. However, the precise reasons for this rapid and drastic increase in population size remain unknown. In order to examine the potential utility of this surplus biomass of Lycorma delicatula, we prepared an aqueous fraction from a dried imago, and tested its various anti-inflammatory activities and effects on cytokine levels in lipopolysaccharide (LPS)-activated RAW264.7 cells. The aqueous extract of Lycorma delicatula showed potent inhibitory activity on nitric oxide production, by more than 60%, at a concentration of 300 g/mL. We also evaluated interleukin (IL)-13 level using enzyme-linked immunosorbent assays (ELISAs). The anti-oxidative fraction of Lycorma delicatula inhibited LPS-induced IL-13 production in a dose-dependent manner, and T-bet promoter activity of cells treated with 300 g/mL of Lycorma delicatula extract was also dramatically inhibited to 62% of that of LPS-treated cells. Furthermore, we compared matrix metalloproteinase-7 (MMP-7), -9, -14 and -17 expressions using reverse transcription-polymerase chain reaction (RT-PCR), which showed a drastic decrease in RNA levels in cells treated with the anti-oxidative fraction. Together, the present results suggest that the aqueous fraction of Lycorma delicatula has potential to ameliorate inflammatory characteristics during an inflammation event, supporting the idea that the fraction may be applied as a biomaterial to prevent inflammatory damage in skin tissues.

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... Together with their nutritional value, evidence show that edible insects contain a huge diversity of other micronutrients and compounds different from fats and proteins, such as fibres, minor lipid compounds, phenolic compounds, alkaloids and other compounds yet to be discovered. In fact, multiple different bioactivities are being reported recently in the literature for edible insects, including anti-inflammatory, antiproliferative, antioxidant, antiangiogenic or antimicrobial, although studies are still scarce [6][7][8][9][10][11][12][13]. ...
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We recently described the cell type distribution of several matrix metalloproteinases (MMP-1 through MMP-16). In this report we extend this study by analysis of three recently described MMPs. PCR primers for MMP-17, MMP-18, and MMP-20 were optimized for use in RT-PCR. The results demonstrate one or more cell lines or tissue that express mRNA for each of these newly described MMPs.
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Superantigens secreted by the bacterial pathogen Staphyloccocus aureus are extremely potent toxins that overstimulate the host immune system by binding to the MHC class II and T cell receptors and activating a large population of T cells. Superantigen infection has been shown to be the causative agents in acute diseases, food poisoning and toxic shock syndrome, and in more chronic conditions such as inflammatory skin diseases. In addition to the toll on public health, S. aureus superantigens also represent a potential biothreat to our national security. To address these risks, a number of different therapeutic strategies have been developed that target different aspects of the pathogenic mechanism of S. aureus and superantigen infection. These therapies, which encompass strategies as diverse as production of neutralizing antibodies, inhibitory peptide/receptor design and blockage of superantigen gene transcription, are being tested for treatment of established S. aureus infections in pre- and post-exposure scenarios. In this review, we will describe these different strategies and their efficacies in inhibition of superantigen-induced effects in the host, and present the future outlook for successfully producing therapies for superantigen-based disease.
Article
While inflammatory blisters have long been utilized as a means of evaluating antimicrobial disposition to aid in the development of new treatments for skin and skin structure infections, sparse data are available regarding the healing of the blisters once the experiment has been completed. We report the blister induction technique and resolution time in ten volunteers enrolled in a pharmacokinetic study using the cantharidin-induced inflammatory blister technique. © 2003 Elsevier Science B.V. and the International Society of Chemotherapy. All rights reserved.
Article
The arolium in Lycorma delicatula is shaped as a truncated pyramid, tapering proximally. The base or the terminal area is corrugated, forming parasagittal wrinkles (period 1.5-5.0 microm), which are supported from inside by cuticular dendrites. Side faces of the arolium are made up of sclerotized dorsolateral plates. When claws slip on a smooth substrate and pronate, the dorsolateral plates diverge and expand the sticky terminal area. The real contact area with the glass plate was recognized by light reflection on its periphery. This area was measured and shown to be smaller when the leg was pressed perpendicularly to the substrate (0.02 mm(2)) than when it was sheared in a direction parallel to the substrate (0.05 mm(2)). Attachment forces were measured with the aid of dynamometric platforms during pulling of active insects from horizontal or vertical glass surfaces. Normal adhesive force (about 9-12 mN) was much less than friction force during sliding with velocity of 6-17 mm/s (50-100 mN); however, when expressed in tenacity per unit contact area the difference was less pronounced: 170 and 375-625 mN/mm(2), respectively. Sliding of the arolium during shear displacement was shown to be oscillatory in frame-by-frame video analysis. Relaxative oscillations consisted of periodical sticks-slips of the arolium along the glass surface.
An extract from Lycorma delicatula for anti-allergic inflammation or treatment to allergic inflammation-related diseases and a cosmetical or pharmaceutical composition comprising the same
  • S H Lee
  • H U Son
Lee SH, Son HU. (2013) An extract from Lycorma delicatula for anti-allergic inflammation or treatment to allergic inflammation-related diseases and a cosmetical or pharmaceutical composition comprising the same. Korea Patent Application #10-2013-0063587, pp. 1-24, June 3.
The effects of cantharidin and cantharidin derivates on tumour cells. Anti-Cancer Agents in Medicinal Chemistry
  • D Liu
  • Z Chen
Liu D, Chen Z. (2009) The effects of cantharidin and cantharidin derivates on tumour cells. Anti-Cancer Agents in Medicinal Chemistry, 9, 392-396.