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Ectomycorrhizal fungal communities in high mountain conifer forests in central Mexico and their potential use in the assisted migration of Abies religiosa

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Abies religiosa forests in central Mexico are the only overwinter refuge of the monarch butterfly and provide important ecosystem services. These forests have lost 55% of their original area and as a consequence, diversity and biotic interactions in these ecosystems are in risk. The aim of this study was to compare the soil fungal diversity and community structure in the Abies religiosa forests and surrounding Pinus montezumae, Pinus hartwegii, and coniferous mixed forest plant communities to provide data on ecology of mycorrhizal interactions for the assisted migration of A. religiosa. We sampled soil from five coniferous forests, extracted total soil DNA, and sequenced the ITS2 region by Illumina MiSeq. The soil fungi community was integrated by 1746 taxa with a species turnover ranging from 0.280 to 0.461 between sampling sites. In the whole community, the more abundant and frequent species were Russula sp. (aff. olivobrunnea), Mortierella sp.1, and Piloderma sp. (aff. olivacearum). The ectomycorrhizal fungi were the more frequent and abundant functional group. A total of 298 species (84 ectomycorrhizal) was shared in the five conifer forests; these widely distributed species were dominated by Russulaceae and Clavulinaceae. The fungal community composition was significantly influenced by altitude and the lowest species turnover happened between the two A. religiosa forests even though they have different soil types. As Pinus montezumae forests have a higher altitudinal distribution adjacent to A. religiosa and share the largest number of ectomycorrhizal fungi with it, we suggest these forests as a potential habitat for new A. religiosa populations.
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ORIGINAL ARTICLE
Ectomycorrhizal fungal communities in high mountain conifer forests
in central Mexico and their potential use in the assisted migration
of Abies religiosa
Andrés Argüelles-Moyao
1,2
&Roberto Garibay-Orijel
1
Received: 13 December 2017 /Accepted: 29 May 2018 /Published online: 11 June 2018
#Springer-Verlag GmbH Germany, part of Springer Nature 2018
Abstract
Abies religiosa forests in central Mexico are the only overwinter refuge of the monarch butterfly and provide important
ecosystem services. These forests have lost 55% of their original area and as a consequence, diversity and biotic
interactions in these ecosystems are in risk. The aim of this study was to compare the soil fungal diversity and
community structure in the Abies religiosa forests and surrounding Pinus montezumae,Pinus hartwegii,andconiferous
mixed forest plant communities to provide data on ecology of mycorrhizal interactions for the assisted migration of A.
religiosa. We sampled soil from five coniferous forests, extracted total soil DNA, and sequenced the ITS2 region by
Illumina MiSeq. The soil fungi community was integrated by 1746 taxa with a species turnover ranging from 0.280 to
0.461 between sampling sites. In the whole community, the more abundant and frequent species were Russula sp. (aff.
olivobrunnea), Mortierella sp.1, and Piloderma sp. (aff. olivacearum). The ectomycorrhizal fungi were the more fre-
quent and abundant functional group. A total of 298 species (84 ectomycorrhizal) was shared in the five conifer forests;
these widely distributed species were dominated by Russulaceae and Clavulinaceae. The fungal community composition
was significantly influenced by altitude and the lowest species turnover happened between the two A. religiosa forests
even though they have different soil types. As Pinus montezumae forests have a higher altitudinal distribution adjacent
to A. religiosa and share the largest number of ectomycorrhizal fungi with it, we suggest these forests as a potential
habitat for new A. religiosa populations.
Keywords Fungal ecology .Beta diversity .Functional groups .Environmental decision-making .Monarch butterfly habitat
Introduction
The high species extinction rate in the Anthropocene (Pievani
2014) is driving to a dramatic loss of lineages constraining the
functionality of ecosystems and reducing our ability to adapt
to a changing environment. In Mexico, the area of primary
temperate forest has been reduced substantially over the pre-
vious 20 years. This has been particularly severe for Pinus and
Quercus species (Miranda-Aragón et al. 2012) and for Abies
religiosa (Ramirez et al. 2015). High altitude conifer forests
are among the most imperiled ecosystems in the Trans-
Mexican Volcanic Belt in central Mexico due to climate
change and deforestation. Temperate forests in these moun-
tains are characterized by an alpine monodominant Pinus
hartwegii forest at elevations above 3500 m, followed by
Pinus montezumae from 3000 to 3500 m and A. religiosa from
2800 to 3000 m.
Electronic supplementary material The online version of this article
(https://doi.org/10.1007/s00572-018-0841-0) contains supplementary
material, which is available to authorized users.
*Roberto Garibay-Orijel
rgaribay@ib.unam.mx
Andrés Argüelles-Moyao
evoandres@gmail.com
1
Laboratorio de Sistemática, Ecología y Aprovechamiento de Hongos
Ectomicorrízicos, Departamento de Botánica, Instituto de Biología,
Universidad Nacional Autónoma de México, Circuito Exterior s/n,
Ciudad Universitaria. Del. Coyoacán, C.P. 04510 Mexico
City, CDMX, Mexico
2
Posgrado en Ciencias Biológicas, Edificio B, 1° Piso, Unidad de
Posgrado, Circuito de Posgrados, Universidad Nacional Autónoma
de México, Ciudad Universitaria, Del. Coyoacán, C.P. 04510 Mexico
City, CDMX, Mexico
Mycorrhiza (2018) 28:509521
https://doi.org/10.1007/s00572-018-0841-0
Content courtesy of Springer Nature, terms of use apply. Rights reserved.
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