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28-Days Hydrogen-Rich Water Supplementation Affects Exercise Capacity in Mid-Age Overweight Women: 2942 Board #225 June 1 3

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Abstract

Molecular hydrogen (H2) improves body composition, metabolic profiles and mitochondrial function in overweight women, yet no studies so far evaluated the effectiveness of H2 for improving exercise capacity in this population. PURPOSE: To examine the effects of 28-days supplementation with 1 L per day of hydrogen-rich water (HRW) on exercise capacity and quality of life in overweight mid-age women. METHODS: Twelve women (age 53.8 ± 13.0 years, BMI 28.8 ± 3.3 kg/m2, VO2max 22.3 ± 3.7 ml/kg/min) participated in this randomized, placebo-controlled, cross-over, repeated-measure interventional study. All participants were allocated in a double-blind design to receive two randomly assigned trials: first group received 1 L per day of HRW (supplying ~ 9 ppm of H2), while the second group received placebo (tap water). Participants were evaluated at baseline, and following 28 days of intervention. The primary endpoint was the change in cardiorespiratory endurance (VO2max) assessed at baseline and at 28 days follow-up. Secondary outcomes included change from baseline to end of treatment in values for work capacity, impact of weight on quality of life (IWQoL), and hematological biomarkers. Participants were asked to maintain their usual lifestyle, dietary intake and not to use other dietary supplements during the study. RESULTS: HRW intervention significantly improved VO2max as compared to placebo at 28-day follow-up (26.2 ± 4.8 ml/kg/min vs. 24.2 ± 4.1 ml/kg/min; P = 0.03). Differences were found for time to exhaustion and total work completed during an incremental exercise, with HRW resulting in improvement of both variables as compared to placebo (P < 0.05). IWQoL scores and hematological markers were not affected by either intervention (P > 0.05). CONCLUSION: Results indicate that HRW can be used as an alternative hydration formulation to positively affect exercise performance in mid-age overweight women. Supported by the Serbian Ministry of Education, Science and Technological Development (175037), the Provincial Secretariat for Higher Education and Scientific Research (114-451-710), the University of Novi Sad Faculty of Sport and PE (2017 Annual Award) and HRW Natural Health Products Inc, New Westminster, BC, Canada. Clinical trial registration www.clinicaltrials.gov, ID number NCT02832219.

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... There are several methods for hydrogen gas administration including: inhalation of H 2 gas (Hayashida et al. 2008), tube feeding of H 2 -rich solution ), intravenous injection of H 2 -rich saline (Cui et al. 2014), H 2 -rich dialysis solution for hemodialysis (Nakayama et al. 2010), hyperbaric H 2 chamber (Dole et al. 1975), bathing in H 2 -rich water (Kato et al. 2012), increasing H 2 production by intestinal bacteria (Chen et al. 2013), topical application , oral ingestion of hydrogen-producing tablets Ostojic et al. 2018 ), and simply drinking hydrogen-rich water (HRW) (Nakao et al. 2010). Regardless of the mode of administration, the cellular bioavailability of molecular hydrogen is extremely high due to its unique physicochemical properties. ...
... Additional randomized, placebo-controlled, cross-over studies suggest that H 2 decreases the rate of perceived exertion and lowers heart rate during submaximal exercise in young healthy adults (n=19) (LeBaron et al. 2019), as well as improves VO 2 max in mid-age overweight women (n=12) (Ostojic et al. 2018). More research on the acute and chronic effects of molecular hydrogen administration and its varying methods of delivery in exercise medicine and sports performance is needed to determine its true efficacy, and from which types of exercises and in which populations the most benefit would occur. ...
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Thesis
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Preprint
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... Interestingly, similar results have also been reported in the previous meeting report. 23 Possible mechanisms underlying the improvement of aerobic capacity by continuous intake of hW Although causation cannot be determined from our results, we can speculate the potential mechanisms that could underlie the increase in VO 2peak during an incremental cycling exercise test by continuous intake of HW. Maximal VO 2 (VO 2max ) or VO 2peak is assumed to be mainly determined by 1) cardiopulmonary function that transports oxygen to the active muscle and 2) mitochondrial oxygen consumption (oxygen extraction and utilisation). ...
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Chapter
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Background: Hydrogen gas (H2 ) has entered the world of experimental therapeutics approximately four and a half decades ago. Over the years, this simple molecule appears to drive more and more scientific attention perhaps due to a dualism of H2 affirmative features demonstrated in numerous in vitro, animal and human studies on one side, and stillpuzzling mechanism(s) of its biological activity on the other. Up to this point, H2 was scrutinized for more than 170 different disease models and pathologies, and many research groups across the world have lately started to dynamically investigate its conceivable performance-enhancing potential. Methods: We outlined here the studies indexed in leading research databases (PubMed, Web of Science, SCOPUS, JSTORE) that explored the effects of hydrogen on exercise performance, and also addressed important restraints, open questions, and windows of opportunities for forthcoming research and possible H2 enactment in exercise physiology. About two dozen trials have been identified in this domain, with most published during the past 5 years, while drinking hydrogen-rich water recognized as the most convenient method to deliver H2 in both animal and human studies. Results: Either administered as an inhalational gas, enteral hydrogen-rich water, or intravenous hydrogen-rich saline, H2 seems to favorably affect various exercise performance outcomes and biomarkers of exercise-associated fatigue, inflammation, and oxidative stress. Not all studies have shown corroborative effects, and it appears that the gold-standard protocol for applying H2 in the field of exercise science does not exist at the moment, with studies markedly differ in the dose of H2 administered, the duration of a treatment, and the source of hydrogen. Conclusion: H2 is a newfangled and rather effective performance-enhancing agent yet its promising ergogenic potency has to be further validated and characterized in more well-controlled, appropriately sampled and long-term mechanistic trials. Also, appropriate regulation of hydrogen utilization in sport as an exotic medical gas may require distinctive legislative actions of relevant regulatory agencies in the future.
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Background: Molecular hydrogen (H2) effectively treats obesity-related disorders in animal models, yet no studies have investigated the effectiveness and safety of H2 for improving biomarkers of obesity in humans. Aim: In this double blind, placebo-controlled, crossover pilot trial, we evaluated the effects of H2 intervention on body composition, hormonal status, and mitochondrial function in ten (n = 10) middle-aged overweight women. Methods: Volunteers received either hydrogen-generating minerals (supplying ~6 ppm of H2 per day) or placebo by oral administration of caplets for 4 weeks. The primary end-point of treatment efficacy was the change in the body fat percentage from baseline to 4 weeks. In addition, assessment of other body composition indices, screening laboratory studies, and evaluation of side effects were performed before and at follow-up. Clinical trial registration www.clinicaltrials.gov , ID number NCT02832219. Results: No significant differences were observed between treatment groups for changes in weight, body mass index, and body circumferences at 4-week follow-up (P > 0.05). H2 treatment significantly reduced body fat percentage (3.2 vs. 0.9%, P = 0.05) and arm fat index (9.7 vs. 6.0%, P = 0.01) compared to placebo administration, respectively. This was accompanied by a significant drop in serum triglycerides after H2 intervention comparing to placebo (21.3 vs. 6.5%; P = 0.04), while other blood lipids remained stable during the study (P > 0.05). Fasting serum insulin levels dropped by 5.4% after H2 administration, while placebo intervention augmented insulin response by 29.3% (P = 0.01). Conclusions: It appears that orally administered H2 as a blend of hydrogen-generating minerals might be a beneficial agent in the management of body composition and insulin resistance in obesity.
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