Article

Sistemas microfisiológicos compostos por organoides humanos em dispositivos microfluídicos: avanços e desafios

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  • UNICAMP - Universidade Estadual de Campinas
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Abstract

Introduction: Models with higher predictive capacity and able to produce results at lower costs and in shorter times are needed for drug development. The microphysiological systems (MPS) that cultivate human tissues in three-dimensional histoarchitecture (3D) are promising alternatives for these objectives. Objective: This review work aims to address the state of the art of SMF development and illustrate the initial Brazilian experience with this technology. Method: The research and data collection covering the theme “Microphysiological Systems”, and the subtopics “Microfluidic Devices” and “3D Culture of Human Cells”, was based on electronic search in Capes Journals Portal, scientific databases Scopus, PubMed and Science Direct and with the Google Scholar search tool. Results: Among the existing microphysiological systems, those that are characterized by the culture of human tissues organized in three - dimensional histoarchitecture in microfluidic devices were recently introduced, as being the most promising ones. In addition, between the years 2000-2017, we recorded approximately increases of 12, 985 and 380 times in the number of academic publications related to the areas of Microfluidics, Organ-on-a-Chip and MPS respectively, illustrating the impact of this technology today. Conclusions: This relatively recent technology has high potential to overcome the limitations of current in vitro experimental models.

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... [46] Furthermore, the possibility of using different cell types in the same organoid allows heterotypic intercellular contacts, providing additional advances in realistically representing tissue functionality and differentiation. [45,47] A summary of charac-teristics from monolayer, spheroids, and organoids are represented in ...
Article
Background An impressive percentage of biomedical advances were achieved through animal research and cell culture investigations. For drug testing and disease researches, both animal models and preclinical trials with cell cultures are extremely important, but present some limitations, such as ethical concern and inability of representing complex tissues and organs. 3D cell cultures arise providing a more realistic in vitro representation of tissues and organs. Environment and cell type in 3D cultures can represent in vivo conditions and thus provide accurate data on cell‐to‐cell interactions, and cultivation techniques are based on a scaffold, usually hydrogel or another polymeric material, or without scaffold, such as suspended microplates, magnetic levitation, and microplates for spheroids with ultra‐low fixation coating. Purpose and scope This review aims at presenting an updated summary of the most common 3D cell culture models available, as well as a historical background of their establishment and possible applications. Summary Even though 3D culturing is incapable of replacing other current research types, they will continue to substitute some unnecessary animal experimentation, as well as complement monolayer cultures. Conclusion In this aspect, 3D culture emerges as a valuable alternative to the investigation of functional, biochemical, and molecular aspects of human pathologies.
... [46] Furthermore, the possibility of using different cell types in the same organoid allows heterotypic intercellular contacts, providing additional advances in realistically representing tissue functionality and differentiation. [45,47] A summary of charac-teristics from monolayer, spheroids, and organoids are represented in ...
... Esses sistemas são capazes de biomimetizar a arquitetura e funções de órgãos e tecidos, tornando-se assim uma alternativa inovadora para a experimentação animal clássica em testes de segurança e eficácia. Cada chip pode ser processado a partir de um biomaterial flexível e translúcido, com dimensões de um cartão de memória, cuja estrutura apresenta canais microfluídicos revestidos por células humanas vivas [122][123][124][125] . Essas características possibilitam ao dispositivo visualizar o funcionamento interno de órgãos e estudo da fisiologia humana em um contexto específico, além de proporcionar o desenvolvimento de modelos in vitro para doenças e a substituição de modelos animais em testes in vivo. ...
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