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Effectiveness of binahong leaves extract, soursop leaves extract and combination of binahong leaves and soursop leaves extract on hemostasis in cuts of mice
Abstract
Objective: Tooth extraction is a process of removing a tooth from the alveolar process which will always cause bleeding. Bleeding is usually occurs as a result of higher pressure in the blood vessels than outside the blood vessel. In Indonesia, many plants used as herbal medicine. Binahong (anredera cordifolia (tenore) steenis) contains flavonoids, terpenoids, essential oils, tannins. Soursop (annona muricata l.) leaf contains flavonoids, triterpenoids, saponins, polyphenols, tannins. The purpose of this study is to determine the effectiveness of binahong leaves extract, soursop leaves and combination of binahong and soursop leaves extract on hemostasis process in cuts of mice.Material and Methods: This research was conducted at the Phytochemicals Laboratory Faculty of Pharmacy and Entomology Laboratory Medical Hasanuddin University. This research is laboratory experimental with post test only with control group design. First stage by making binahong and soursoup leaves extract using maceration techniques. Male mice were adapted for seven days. Treatment on day eight by cutting the tail as much as 1 cm from the tip of the tail. Bleeding time is calculated after dipping tip of tail into the extract for 5 seconds until no blood dripping on gauze pads. Data were analyzed using kruskal wallis test followed by mann-whitney test.Results: Binahong leaves extract, soursop leaves extract and combination of binahong and soursop leaves extracts affected hemostasis on cuts of male mice (mus musculus).Conclusion: Combination binahong and soursop leaves extracts have the best effectiveness.
... 20 Selain tanin, flavonoid dapat menjaga permeabilitas pembuluh darah, meningkatkan resistensi pembuluh darah, meningkatkan fungsi endotel dan mengurangi tekanan oksidatif. 22 Fungsi endotel tersebut akan berdampak pada fungsi dan aktivitas trombosit serta fungsi sel otot pembuluh darah. 23 Flavonoid secara in-vivo terbukti memiliki sifat anti inflamasi dengan menghambat pelepasan asam arakidonat. ...
ABSTRAK Pendahuluan: Pencabutan gigi adalah tindakan dalam kedokteran gigi yang sering dilakukan. Komplikasi yang dapat terjadi dalam pencabutan gigi, salah satunya adalah perdarahan. Biji buah alpukat memiliki potensi sebagai agen hemostatis yang dapat berpengaruh teradap waktu perdarahan. Dari hasil skrining fitokimia, ekstrak etanol biji buah alpukat mengandung saponin, flavonoid dan tanin. Penelitian ini bertujuan untuk mengetahui apakah ekstrak biji alpukat dapat mempengaruhi waktu perdarahan pada mencit. Metode: Jenis penelitian eksperimental laboratoris dengan rancangan post-test only control group design . Sampel penelitian adalah mencit jantan sebanyak 16 ekor yang dibagi menjadi 4 kelompok. Kelompok kontrol negatif (K1) diberikan akuades, kelompok kontrol positif (K2) diberikan asam traneksamat, kelompok perlakuan 1 (P1) diberikan ekstrak biji alpukat dosis 0,125 mg/g BB dan kelompok perlakuan 2 (P2) diberikan ekstrak biji alpukat dosis 0,25 mg/g BB. Hewan coba diadaptasikan selama 7 hari, diberikan perlakuan selama 7 hari dan pada hari ke-8 dilakukan pemotongan ekor ±1 cm. Data dianalisis menggunakan uji Kruskall Wallis dan Mann Whitney . Hasil: Rerata waktu perdarahan pada kelompok K1, K2, P1 dan P2 adalah 7,8 menit, 3,25 menit, 3,8 menit dan 4,5 menit. Hasil uji Kruskal Wallis didapatkan signifikansi 0,029 (p<0,05) yaitu terdapat perbedaan pada seemua kelompok. Hasil uji Mann Whitney didapatkan perbedaan signifikan antara kelompok K1, P1 dan P2 (p<0,05), sedangkan pada kelompok P1 dan P2 (p>0,05) terdapat perbedaan yang tidak signifikan. Simpulan: Ekstrak biji alpukat dapat mempercepat waktu perdarahan pada luka potong ekor mencit. Kata kunci: Persea americana Mill.; waktu perdarahan; trombosit ABSTRACT Introduction: Tooth extraction is a common treatment form in dental treatment. Complications can occur in tooth extraction, one of which is bleeding. Avocado seeds have the potential as haemostatic agents that can affect bleeding time. From the results of phytochemical screening, the ethanol extract of avocado seeds contains saponins, flavonoids and tannins. This study was aimed to determine the effect of avocado (Persea americana Mill.) seed extract on bleeding time in the tail cuts of mice (Balb-c strain). Methods: This research was an experimental laboratory with a post-test only control group design. The research sample was 16 male mice divided into four groups. The negative control group (K1) was administered with distilled water; the positive control group (K2) with tranexamic acid; treatment group 1 (P1) with avocado seed extract at a dose of 0.125 mg/g BW; treatment group 2 (P2) with avocado seed extract at a dose of 0.25 mg/g BW. The experimental animals were adapted for seven days, treated for seven days and on the eighth day, the tail was cut for ±1 cm. Data were analysed using the Kruskal Wallis and Mann Whitney tests. Results: The mean bleeding time in groups K1, K2, P1, and P2 were 7.8 minutes, 3.25 minutes, 3.8 minutes, and 4.5 minutes, respectively. The Kruskal Wallis test results showed a significance of 0.029 (p<0.05), thus showing differences in all groups. The Mann Whitney test results showed a significant difference between groups K1 and P1 and P2 (p<0.05). While between the groups P1 and P2, no significant difference was found (p>0.05). Conclusions: Avocado seed extract can accelerate the bleeding time in the mice tail cuts. Keywords: Persea americana Mill. ; bleeding time; platelets
Objective: To observe the effect of 3% binahong leaves extract gel on wound healing of socket post tooth extraction. Methods: Twenty-four wistar strain white rats (Rattus novergicus) were used in this experimental laboratory work, and the 3% extract gel of binahong plants was created from scratch in the Pharmacognition Laboratory. A 3% gel made from the extract of binahong leaves was made in the Pharmacognition Laboratory of the Faculty of Pharmacy, Universitas Sumatera Utara, Medan. The gel was applied twice a day, in the morning and the evening, to the socket wounds using a bent needle irrigation tip. Each rat were subjected to dental extraction of the mandibular left incisor and divided into three different groups; the negative control group, positive control group, and the treatment group with each of the socket group observed every three and seven days and the cells were colored using Hematoxilin-Eosin method. Following data collection, the SPSS Statistics program was used to analyze the data. Results: Clinically, socket wounds were visibly smaller whereas microscopically the distance of the wound edges greatly reduced in both groups. Histologically fibroblast proliferation increased after application of binahong extract, but there was no significant difference on the 3rd and 7th d after extraction. Conclusion: Application of binahong leaf extract gel in the socket following tooth extraction, the binahong 3% extract gel contributes to the acceleration of fibroblast proliferation.
Soursop leaf has been used traditionally to treat a variety of diseases, because soursop leaf contain antioxidant compound. The research objective to be achieved is to measure and analyze the activity of antioxidant and organoleptic properties of Soursop Leaf Tea by variations in drying time 30, 60, 90, 120, and 150 minutes. Measurement of antioxidant activity using UV-Vis spectrophotometry method (λ 517 nm), whereas the organoleptic parameters : taste, color, aroma, and appearance. Result of studies that a treatment time of drying effect on antioxidant activity of Soursop Leaf Tea. Soursop leaf drying conditions at 50 o C with a temperature of 150 minutes give the highest level of antioxidant activity and the lowest EC50 value, but it has lowest a flavor organoleptic. Recommendations, drying temperature of 50 o C tailings with drying 150 minutes, and to increase flavor can be done with the added the essen. PENDAHULUAN Tanaman sirsak (Annona muricata Linn.) berasal dari bahasa Belanda, yakni zuurzak berarti kantong asam. Daun sirsak banyak digunakan sebagai obat herbal untuk mengobati berbagai penyakit, antara lain : penyakit asma di Andes Peru, diabetes dan kejang di Amozania Peru (Zuhud, 2011). Kandungan senyawa dalam daun sirsak antara lain steroid/terpenoid, flavonoid, kumarin, alkaloid, dan tanin. Senyawa flavonoid berfungsi sebagai antioksidan untuk penyakit kanker, anti mikroba, anti virus, pengatur fotosintetis, dan pengatur tumbuh (Robinson, 1995). Masyarakat Indonesia menggunakan daun sirsak sebagai obat herbal untuk mengobati penyakit kanker, yaitu dengan cara meminum air rebusan daun sirsak segar. Air rebusan daun sirsak segar dapat menimbulkan efek panas seperti pada kemoterapi, namun air rebusan daun sirsak ini hanya membunuh sel-sel yang abnormal (kanker) dan membiarkan sel-sel normal tetap tumbuh. Hal ini berbeda dengan efek yang ditimbulkan pada pengobatan kemoterapi, dimana pengobatan kemoterapi ini tidak saja membunuh sel-sel abnormal (kanker) tetapi sel-sel yang normalpun ikut mati (Leny, 2006). Meskipun air rebusan daun sirsak segar telah lama digunakan sebagai obat herbal untuk
Accelerating wound healing after tooth extraction is beneficial in dental treatment. Application of antioxidants, such as reduced coenzyme Q10 (rCoQ10), may promote wound healing after tooth extraction. In this study, we examined the effects of topical application of rCoQ10 on wound healing after tooth extraction in rats. After maxillary first molars were extracted, male Fischer 344 rats (8 weeks old) (n = 27) received topical application of ointment containing 5% rCoQ10 (experimental group) or control ointment (control group) to the sockets for 3 or 8 days (n = 6-7/group). At 3 days after extraction, the experimental group showed higher collagen density and lower numbers of polymorphonuclear leukocytes in the upper part of socket, as compared to the control group (p < 0.05). Gene expression of interleukin-1β, tumor necrosis factor-α and nuclear factor-κB were also lower in the experimental group than in the control group (p < 0.05). At 8 days after tooth extraction, there were no significant differences in collagen density, number of polymorphonuclear leukocytes and bone fill between the groups. Our results suggest that topical application of rCoQ10 promotes wound healing in the soft tissue of the alveolar socket, but that rCoQ10 has a limited effect on bone remodeling in rats.
Tannins are obtained upon the decomposition of vegetation. They will generally be found in surface water supplies or shallow wells. Although these compounds are not a health risk, they are aesthetically displeasing. Tannins are difficult to remove from water. Tannins can cause a yellow to brown cast in water and may also affect a taste and odour.
Wound caused by trauma poses challenging daily clinical problems for physicians in hospitals and other health services. It has high risk for mortality and morbidity. Binahong [Anredera Cordifolia (Ten.) Steenis] plant originates from China and can be found abundantly in Indonesia. The objective of this research is to investigate the effect of topical application of Binahong leaf paste on wound healing process.
Methods: A laboratory experimental study was conducted at the Pharmacology Laboratory, Universitas Padjadjaran, from October to December 2012 by using 27 Mus Muscularis mice which were divided into 3 groups: group 1 (sodium chloride 0.9%), group 2 (Binahong) and group 3 (povidone iodine 5%). Binahong leaves were crushed and were mixed with water to make a paste. The application was given for 12 days. On day zero, 1.0 cm2 of wound was incised at the back of the mice, and further on day 1, 4, 8 and 12, the percentage of wound contraction was measured. The data was analyzed using Mann-Whitney test.
Results: The study indicated that the percentage of wound contraction in group 2 on day 4 (1.17%), day 8 (23.7%) and day 12 (76.14%) is the highest among all groups. The result showed that the comparison between binahong and NaCl was highly significant on day 12, p= 0.001 (p < 0.05) and the data for comparison between Binahong and povidone iodine on day 12 was significant with p= 0.003 (p < 0.05).
Conclusions: Topical application of Binahong leaf paste shows better result in wound healing process of Mus muscularis mice. From this study it can be concluded that binahong promotes wound healing and can be used for home remedies for wound care and as an alternative traditional way to treat wound.[AMJ.2014;1(1):6–11]
Green tea is one of the most popular and extensively used dietary supplement in the United States. Diverse health claims have made for green tea as a trendy ingredient in the growing market for nutraceuticals and functional foods. Green tea extract contains several polyphenolic components with antioxidant properties, but the predominant active components are the flavanol monomers known as catechins, where epigallocatechin-3-gallate and epicatechin-3-gallate are the most effective antioxidant compounds. Additional active components of green tea extract include the other catechins such as epicatechin and epigallocatechin. Among these, epigallocatechin-3-gallate is the most bioactive and the most scrutinized one. Green tea polyphenols are also responsible for distinctive aroma, color and taste. Green tea extract can also be used in lipid-bearing foods to delay lipid oxidation and to enhance the shelf-life of various food products. This review outlines the chemistry, flavour components, antioxidant mechanism, regulatory status, food applications, and stability of green tea extract in food.
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