Content uploaded by Josman Palmeira
Author content
All content in this area was uploaded by Josman Palmeira on Mar 26, 2018
Content may be subject to copyright.
Evaluation of the antimicrobial activity in vitro of the hydroalcoholic extract Stryphnodendron adstringens against of Staphylococcus aureus strains
Abstract
Staphylococcus aureus is an important causative agent associated with acquired infections, which is highlighted by their
frequency and their high pathogenicity, and he was able to quickly develop resistance to antibiotics, and constitute a serious threat to
public health. The plants have been a valuable source of products for maintenance of human health and considered one of the best
sources to provide a variety of drugs, among them is the Stryphnodendron adstringens (barbatimão) which, among other actions,
antimicrobial activity. The antimicrobial activity was determined by the method of dissemination in the midst solid, cavity-plate
process and the minimum inhibitory concentration was calculated considering the lowest concentration of the extract can inhibit bacterial
growth after hatching to 37 degrees Celsius for 24 hours. The extract hidroalcóolico of barbatimão was tested front of the crude
extract (100%) and the dilution in concentrations 50%, 25%, 12.5%, 6.25%, 3.12%, 1.56% and 0.78 %, Where it was observed that all
tested strains were sensitive to extract, where the largest halo of inhibition of bacterial growth was 22 mm in crude extract (100%) and
less than 8mm in the dilution of 1:64 (1,56%).
... Although the halo after 24 h for alcoholic extract is slightly larger than that of the aqueous extract, after 48 h is possible to observe the appearance of some colony in the region of the halo for alcoholic extract. This find was expected, since other studies have shown that S. barbatiman has antimicrobial activity against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis, Candida albicans, Candida krusei, Enterococcus faecalis, Klebsiella pneumoniae, Kocuria rhizophila, Neisseria gonorrhoeae, and Shigella flexneri [7]. This effect can be attributed to high tannin content, which is able to inhibit bacterial adhesion and enzymes [7]. Figure 2 shows AFM images for S. barbatiman, C. freundii and C. freundii dispersed in S. Barbatiman aqueous extract at different times of contact. ...
... This find was expected, since other studies have shown that S. barbatiman has antimicrobial activity against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis, Candida albicans, Candida krusei, Enterococcus faecalis, Klebsiella pneumoniae, Kocuria rhizophila, Neisseria gonorrhoeae, and Shigella flexneri [7]. This effect can be attributed to high tannin content, which is able to inhibit bacterial adhesion and enzymes [7]. Figure 2 shows AFM images for S. barbatiman, C. freundii and C. freundii dispersed in S. Barbatiman aqueous extract at different times of contact. ...
Medicinal plants have been presented as a valuable source of preservation of human health. In special, Stryphnodendron barbatiman has been employed due to its antimicrobial activity. This plant is rich in tannins and has been used in popu-lar medicine for the treatment of gastrointestinal disorder, treatment of lesions, and also as anti-inflammatory microbi-cide. Citrobacter freundii is a member of the family Enterobacteriaceae and is one of the major causes of opportunistic infections. This microorganism is a bacterium (bacillus) aerobic gram-negative with a length in the range of 1 to 5 m. C. freundii is commonly found in water, soil, food and occasionally in the gastrointestinal tract of animals and humans. In this paper, we have demonstrated the antibacterial activity of S. barbatiman by observing cellular death by using in-hibition halo approach. Atomic force microscopy and FT-IR spectroscopy results suggested that interaction between the main active components of S. barbatiman with cellular wall of C. freundii gives rise to cellular wall damage, and then leads this microorganism to death.
... Empirically, S. adstringens has been used as an antiseptic and antimicrobial as well as for treatments of diabetes, inflammation, gastritis, liver disease, pain, diarrhea, and bleeding (Costa et al., 2013;De Santana et al., 2016). Scientifically, some studies demonstrate that the aqueous extract of S. adstringens has antioxidant and anticancer properties (da Silva Baldivia et al., 2018), antifungal, including action against fluconazole-resistant isolates (de Freitas et al., 2018), pronounced anti-inflammatory activity (Henriques et al., 2016), and antimicrobial action (Costa et al., 2010;Ferreira, Palmeira, Souza, Almeida, Figueiredo, Pequeno, Arruda, 2010). This fact is important because several oral diseases have a dysbiotic microbial biofilm as the main etiological factor. ...
Stryphnodendron adstringens (Mart.) Coville is a medicinal plant known for its anti-inflammatory and antimicrobial properties. This study evaluated some biological activities of extract from S. adstringens. Antimicrobial activity was checked in planktonic cultures and monomicrobial biofilms on aerobic, and anaerobic dental microorganisms. Analyzes of cytotoxicity using MTT assay, and genotoxicity by micronucleus test were performed in human keratinocytes (HACAT), murine macrophages (RAW 264.7), and murine fibroblasts (L929). The anti-inflammatory effect was checked in RAW 264.7 stimulated by lipopolysaccharide (LPS) from Escherichia coli, and treated with the plant extract. The levels of cytokines, and nitric oxide (NO) were measured by ELISA, and Griess method, respectively. Data were analyzed by ANOVA, followed by Tukey's, or Kruskal-Wallis, and Dunns tests (P ≤ 0.05). Biofilms of anaerobic bacteria were very susceptible to the plant extract. Effective concentrations showed cell viability > 50%, except 25 mg/mL for HACAT after 24 h of exposure. The extract of S. adstringens was not genotoxic for RAW 264.7. LPS associated with extract increased the production of all cytokines, except TNF-α. However, the plant extract decreased the production of NO. In conclusion, the extract of S. adstringens affected biofilm of anaerobic bacteria using non-cytotoxic concentrations for RAW 264.7, L929, and HACAT cells.
... The antimicrobial effects of tannin rich plant extract against NH 3 producing E. coli, Klebsiella spp. and Pseudomonas spp. was reported by Ferreira et al. (2010). Powell et al. (2011) reported that feeding tannin extract can reduce urine N excretion and urease activity in dairy feces. ...
The fruit Punica granatum L. has been used for years in traditional medicine owing to the presence of several phytobiotics with antimicrobial and immunomodulatory properties. This study investigated the effects of dietary supplementation with Punica granatum L. by-products (PGB) on performance, immunity, intestinal and excreta microflora, and odorous gas emissions from excreta of broiler chickens. Three experimental diets containing 0, 0.5 and 1.0 % PGB were fed to 240 one-day-old broiler chicks until 35 days. Dietary PGB linearly reduced the average daily feed intake and feed conversion ratio of broilers. Supplementation with 1 % PGB led to a linear increase in the relative weight of the spleen and bursa of Fabricius. The concentration of serum IgA and IgG increased linearly in response to dietary PGB. In the ileal digesta, the concentration of Saccharomyces cerevisiae increased linearly and quadratically in response to dietary PGB. Moreover, dietary PGB led to a linear decrease in Escherichia coli and Salmonella spp. alongside reducing the pH of the ileal digesta. In the cecal digesta, the concentration of Bacillus bacteria increased linearly in response to both levels of dietary PGB, while the concentrations of E. coli and Salmonella decreased when the diet was supplemented with 1 % PGB, as did cecal pH. At 35 day, both levels of PGB increased the concentration of fecal Bacillus, whereas only 1 % PGB increased the concentration of S. cerevisiae at 21 day. Increasing levels of PGB induce a linear reduction in fecal E. coli at 21 and 35 day, whereas Salmonella only at 21 day. Regarding the average of 48 h, dietary PGB effectively reduced the emissions of ammonia and methanethiol from broiler excreta. In conclusion, the results suggest that, dietary PGB improved immunity and the intestinal microbial ecosystem of broilers along with reduced odorous gas emissions from excreta.
ResearchGate has not been able to resolve any references for this publication.