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Petrocodon asterocalyx, a new species of Gesneriaceae from Guangxi, China

Authors:
  • Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences
  • Guangxi Institute of Botany, CAS

Abstract and Figures

Petrocodon asterocalyx F.Wen, Y.G.Wei & R.L.Zhang, a new species from the Danxia landform area in Guangxi, South China, is described and illustrated based on molecular and morphological data. The molecular evidence shows that the new species is recovered in a weakly supported clade. Within this clade, the new one is morphologically similar to P. hancei (Hemsl.) A.Weber & Mich.Möller and P. coriaceifolius (Y.G.Wei) Y.G.Wei & Mich.Möller, and it can be distinguished from the former by calyx lobes 20–40 × 2–3 mm, corolla 2.5–3.0 cm long, filaments sparsely erectly pubescent, anthers sparsely pubescent, staminodes 3, and stigmas 2; from latter by leaf blades rhombic-oblong or rhombic, base shallowly cuneate, margin crenulate to serrate, calyx lobes linear, 20–40 × 2–3 cm, and anthers 3.5–3.8 mm long, sparsely pubescent and elliptical.
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Phytotaxa 343 (3): 259–268
http://www.mapress.com/j/pt/
Copyright © 2018 Magnolia Press Article PHYTOTAXA
ISSN 1179-3155 (print edition)
ISSN 1179-3163 (online edition)
Accepted by Hai He: 11 Feb. 2018; published: 12 Mar. 2018
https://doi.org/10.11646/phytotaxa.343.3.6
259
Petrocodon asterocalyx, a new species of Gesneriaceae from Guangxi, China
RUI-LI ZHANG1, LONG-FEI FU2, 3, SHU LI2, 3, YI-GANG WEI2, 3, STEPHEN MACIEJEWSKI2, 4, MICHAEL
LOFURNO5 & FANG WEN2, 3, *
1College of Landscape Architecture,Beijing University of Agriculture, Beijing Laboratory of Urban and Rural Ecological Environment,
Beijing Engineering Research Center of Rural Landscape Planning and Design, CN-102206, Beijing, P.R. China.
2Guangxi Key Laboratory of Plant Conservation and Restoration Ecology in Karst Terrain, Guangxi Institute of Botany, Guangxi
Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin, CN-541006, P.R. China.
3Gesneriad Conservation Center of China (GCCC), Guilin Botanical Garden, No. 85, Yanshan Town, Guilin, CN-541006, P.R. China.
4The Gesneriad Society, 2030 Fitzwater Street, Philadelphia, PA 19146, USA.
5Landscape Architect, Philadelphia. PA 19146, USA.
*Corresponding author: wenfang760608@139.com
Abstract
Petrocodon asterocalyx F.Wen, Y.G.Wei & R.L.Zhang, a new species from the Danxia landform area in Guangxi, South
China, is described and illustrated based on molecular and morphological data. The molecular evidence shows that the new
species is recovered in a weakly supported clade. Within this clade, the new one is morphologically similar to P. hancei
(Hemsl.) A.Weber & Mich.Möller and P. coriaceifolius (Y.G.Wei) Y.G.Wei & Mich.Möller, and it can be distinguished from
the former by calyx lobes 20–40 × 2–3 mm, corolla 2.5–3.0 cm long, filaments sparsely erectly pubescent, anthers sparsely
pubescent, staminodes 3, and stigmas 2; from latter by leaf blades rhombic-oblong or rhombic, base shallowly cuneate,
margin crenulate to serrate, calyx lobes linear, 20–40 × 2–3 cm, and anthers 3.5–3.8 mm long, sparsely pubescent and el-
liptical.
Key words: Danxia landform, endemism, flora of Guangxi, new taxon, taxonomy
Introduction
Petrocodon Hance (1883: 167) originally was a small endemic genus in China, with three species and one variety:
P. dealbatus Hance (1883: 167), P. dealbatus var. denticulatus (W.T.Wang 1975: 101) W.T.Wang (1990: 420), P.
ferrugineus Y.G.Wei (2007: 135) and P. multiflorus F.Wen & Y.S.Jiang in Jiang et al. (2010: 57) before 2011. Wang
et al. (2011) preliminarily revised Petrocodon and its relative genera in early 2011. However, only four species from
four monospecific genera, Petrocodon, Calcareoboea C.Y.Wu ex H.W.Li (1982: 241), Tengia Chun (1946: 279) and
Paralagarosolen Y.G.Wei (2004: 528), were collected and studied. It resulted in the conclusion of revision for previous
Petrocodon sensu stricto and its congeners were imperfect because of lacking some necessary and sufficient samples.
Soon afterwards, Weber et al. (2011b) revised Petrocodon again late in 2011. The newly defined Petrocodon sensu
lato has been swelled to contain at least 31 species and one variety by incorporating some Chinese monotypic genera,
i.e. Calcareoboea, Dolicholoma D.Fang & W.T.Wang in Wang (1983: 18), Paralagarosolen and Tengia, some species
of two Chinese endemic genera: Lagarosolen W.T.Wang (1984b: 12) (six species), Wentsaiboea D.Fang & D.H.Qin
(2004: 533) (one species), four species of Didymocarpus Wallich (1819: 378) and one species of Primulina Hance
(1883: 169) (P. guangxiensis Yan Liu & W.B.Xu in Liu et al. (2011: 1)) as well (Xu et al. 2014). It also includes 13
species described after 2011 (Möller et al. 2016, Lu et al. 2017a & 2017b).
Almost all known species can be found in limestone areas, with one notable exception, Petrocodon hancei
(Hemsley 1890: 299) A.Weber & Mich.Möller in Weber et al. (2011b: 59). It grows on all kinds of substrates from
different parent rocks, such as limestone, sandstone, shale and glutenite. When we investigated the transition areas
of limestone and Danxia areas in Lechang city, Guangdong (Liu & Huang 1991), we observed many populations of
P. hancei from limestone areas, transition zones and Danxia areas. Thus, when we collected some specimens of an
unknown species of Petrocodon without flowers from the Danxia area in Ziyuan County, Guangxi in 2005 (Huang et
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260 Phytotaxa 343 (3) © 2018 Magnolia Press
al. 2015a & 2015b), we improperly identified it as P. hancei due to the similar characters. To our surprise, it appeared
different from P. hancei when the flowering specimens were collected in May 2012. After consulting the national floras
(Wang 1990, Wang et al. 1998), monographs (Li & Wang 2004, Wei et al. 2010) and relevant literature (e.g. Möller et
al. 2016, Cen et al. 2017, Lu et al. 2017a) as well as herbarium species from ANU, BJFU, CDBI, CTC, GH, HN, IBK,
IBSC, KUN, NU, PH, PE, US and VMN, we considered that it is an undescribed species of Petrocodon. In trying to
avoid the misidentification of this new species, we focused on potentially confusing morphological convergence and
undertook molecular analysis.
Material and methods
Morphometric analysis:—Detailed studies on fresh plants and dried specimens of Petrocodon asterocalyx from the
herbarium IBK were undertaken. Morphological data used for the description and studies on their variation were also
obtained from in situ examination of all living mature plants from the population in Ziyuan county, northern Guangxi,
China. For light microscopy (LM), thirty fresh samples (flowers) from 30 mature individuals were fixed in absolute
alcohol-acetic acid (3:1 v/v), and herbarium materials were re-hydrated in distilled water with a drop of soap, for 30
min. All observations of specimens and fixed flowers were carried out using the Olympus BH2-RFCA microscope
(Tokyo, Japan).
Genomic DNA extraction, PCR amplification and sequencing:—To confirm the identity and systematic
placement of this new plant, we performed phylogenetic inference of DNA sequence data obtained from the Internal
Transcribed Spacer (ITS) region and the plastid trnL-F intron spacer (trnL-F). The new species and its related species
were sampled based on previous molecular phylogenetic studies of Gesneriaceae (Möller et al. 2009 & 2011, Weber
et al. 2011a & 2011b). Methods used for DNA extraction, PCR amplification and sequencing were carried out as
described by (Wei et al. 2013). To elucidate the phylogenetic affinities of the new species, 17 species of Petrocodon
including this new species were taken as ingroup, and Primulina dryas (Dunn in Dunn & Tutcher 1912: 195) Mich.
Möller & A.Weber in Weber et al. (2011a: 782) and P. pinnata (W.T.Wang (1984a: 25) Y.Z.Wang in Wang et al. (2011:
62) as outgroup based on recent phylogenetic analyses (Möller et al. 2009 & 2011, Weber et al. 2011a & 2011b, Yu et
al., 2015). Sequences obtained from this study and NCBI GenBank are listed in Table 1.
TABLE 1 Species names, voucher numbers and accession numbers of ITS and trnL-F sequences used in this study.
Species name (according to Weber et al., 2011) Voucher number trnL-F ITS
Petrocodon ainsliifolius W.H.Chen & Y.M.Shui HQ632941 HQ633038
Petrocodon asterocalyx F.Wen, Y.G.Wei & R.L.Zhang WF120510 KC904957 KC904954
Petrocodon coccineus (C.Y.Wu ex H.W.Li) Y.Z.Wang FJ501516 FJ501341
Petrocodon coriaceifolius (Y.G.Wei)Y.G.Wei & Mich.Möller HQ632943 HQ633040
Petrocodon dealbatus Hance GU350668 GU350636
Petrocodon ferrugineus Y.G.Wei HQ632946 HQ633043
Petrocodon hancei (Hemsl.)A.Weber & Mich.Möller HQ632944 HQ633041
Petrocodon hechiensis (Y.G.Wei, Yan Liu & F.Wen) Y.G.Wei & Mich.
Möller HQ632942 HQ633039
Petrocodon hispidus (W.T.Wang) A.Weber & Mich.Möller HQ632939 HQ633036
Petrocodon integrifolius (D.Fang & L.Zeng) A.Weber & Mich.Mölle HQ632940 HQ633037
Petrocodon lithophilus Y.M. Shui, W.H. Chen & Mich. Möller KF202302 KF202295
Petrocodon longgangensis W.H. Wu & W.B. Xu KC765116 KC765114
Petrocodon lui (Yan Liu & W.B.Xu) A.Weber & Mich. Möller HQ632938 HQ633035
Petrocodon niveolanosus (D.Fang & W.T.Wang) A.Weber & Mich.
Möller JF697588 JF697576
Petrocodon scopulorum (Chun) Y.Z.Wang HQ632947 HQ633044
Petrocodon tiandengensis (Yan Liu & B.Pan) A.Weber & Mich.Möller HQ632945 HQ633042
Petrocodon viridescens W.H. Chen, Mich. Möller & Y.M. Shui HQ632939 HQ633036
Primulina dryas (Dunn) Mich. Möller & A. Weber FJ501524 FJ501348
Primulina pinnata (W.T. Wang) Y.Z. Wang FJ501526 FJ501349
Sequence alignment and phylogenetic analysis:—Sequence data were edited and assembled using Lasergene
Navigator 7.1 (DNAstar, Madison, Wisconsin, USA). Cleaned sequences were aligned with the MEGA 5.1 (Tamura et
al. 2011) with additional manual refinements where necessary. Phylogenetic analyses were conducted using maximum
PETROCODON ASTEROCALYX Phytotaxa 343 (3) © 2018 Magnolia Press 261
parsimony (MP) method implemented in PAUP* 4.0b10. Heuristic searches were performed using a starting tree built
from stepwise addition with TBR branch swapping and 1,000 random addition replicates. To assess confidence in
clades, bootstrap analyses based on 1,000 replicates with 10 random additions per replicate were used.
Molecular analysis results
The combined molecular dataset for 19 species comprised a total of 1522 characters: 678 from ITS, and 844 from
trnL-F. Of these, 196 (12.88%) were variable and 115 (7.56%) were parsimony informative. Maximum parsimony
(MP) analysis of the combined data generated five most parsimonious trees (tree length = 483 steps, consistency index
= 0.787, retention index = 0.632). The topology of MP tree (Fig.1) was similar to previous molecular phylogenetic
studies (Möller et al. 2009 & 2011, Weber et al. 2011). However, the clade including Petrocodon asterocalyx was
weakly resolved (BS = 53). This clade includes P. hancei, P. coriaceifolius (Y.G.Wei 2006: 273) Y.G.Wei & Mich.
Möller in Weber et al. (2011b: 59), P. dealbatus, P. ferrugineus and P. scopulorum (Chun 1946: 281) Y.Z.Wang in
Wang et al. (2011: 60) as: P. scopulorus’. Of these, the new species was morphologically most similar to P. hancei
and P. coriaceifolius.
FIGURE 1 The strict consensus tree resulted from a Maximum-parsimony (MP) analysis based on combined trnL-F and ITS sequences of
19 species. Bootstrap values >50% by MP analysis are given below branches. indicates the new species, Petrocodon asterocalyx.
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262 Phytotaxa 343 (3) © 2018 Magnolia Press
Taxonomic treatment
Petrocodon asterocalyx F.Wen, Y.G.Wei & R.L.Zhang, sp. nov. (Figs. 2 & 3 A–F)
Diagnosis:—Petrocodon asterocalyx is related to P. hancei and P. coriaceifolius, but it differs from the former by the calyx lobes 20–40
× 2–3 mm, corolla 2.5–3.0 cm long, filaments sparsely erectly pubescent, anthers sparsely pubescent, staminodes 3, and stigmas 2,
from the latter by leaf blades rhombic-oblong or rhombic, base shallowly cuneate, margin crenulate to serrate, calyx lobes linear,
20–40 × 2–3 mm, and anthers 3.5–3.8 mm long, sparsely pubescent and elliptical.
Type:CHINA. Guangxi: Ziyuan County, Meixi Town, Tianmenshan, in crevice on the rock face, 24°20’24.81”N, 109°12’33.3036”E,
elev. ca. 130 m, 10 May 2012 (fl.), F. Wen WF120510 (holotype IBK!; isotype IBK!)
FIGURE 2 Petrocodon asterocalyx. (A) habitat, (B) the adaxial leaf, (C) the abaxial leaf blade, (D) top view of flower, (E) lateral view of
flower, (F) opened corolla for showing stamens and staminodes, (G) lateral view of anthers, (H) opened anthers, (I) pistil, and (J) stigma
(Drawn by Ms. Xuan Wen based on holotype, F. Wen WF120510).
PETROCODON ASTEROCALYX Phytotaxa 343 (3) © 2018 Magnolia Press 263
FIGURE 3 Petrocodon asterocalyx. (A) habitat, (B) habit, (C) cymes, (D) the lateral view of flower and bud, (E) top view of flower, (F)
stigma, (G) opened corolla, and (H) anthers and filaments.
Perennial herb. Rhizome subterete, stemless, 1.5–5 cm long, 0.3–0.5 cm in diam. Leaves 6–10 or more, all basal, with
petiole, leaf blade symmetric or slightly asymmetric in each pair, coriaceous, fragile and easily broken, rhombic-oblong
or rhombic, 6.5–13.5 × 2–6 cm, apex obtuse or acute, base shallowly cuneate, margin crenulate to serrate, 5–7-nerved
on each side; petiole 1.5–5.5 cm long or longer; petiole, peduncle and pedicel sparsely hispid, with unapparent nodules,
green. Cymes 1–5, 4–20- flowered; peduncle purple, 3–10 cm; bracts 2, opposite, linear, entire, 10–15 × 1.5–2.0 mm,
glabrous; bracteoles similar to bracts, 6–9 × 1–1.2 mm; pedicel 10–20 mm long. Sepals 5-sect, actinomorphic from
the base, linear, 2–4 × 0.2–0.3 cm, outside sparsely strigose, inside glabrous. Corolla pinkish purple, throat with half
dark-purple ring, two or three dark-purple longitudinal stripes on each one of lower lip lobe; corolla 2.5–3.0 cm long,
extremely short pubescent hairs, tube infundibuliform, 1.2–1.3 cm long, 6–8 mm in diam. in the orifice, ca. 2.5 mm in
diam. in the base, abaxial lip ca. 7 mm long, 2-bilobed, lobes triangular, adaxial lip 1.8–2 cm long, split to the middle,
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lateral lobes obliquely triangular, ca. 4.5 mm long, 6.5–6.8 mm in diam. at base. Stamens 2, adnate to 1.0–1.1 cm above
base of corolla tube; filaments dark purple, linear, 7.8–8.5 mm long, pubescent; anthers dark purple, with longitudinal
white stripe at the center, sparsely pubescent, elliptical, 3.5–3.8 mm long, staminodes 3, glabrous, lateral ones adnate
ca. 4 mm above base of corolla tube, ca. 0.7 mm long, median adnate ca. 3 mm above base of corolla tube, ca. 0.3 mm
long. Disc about 1.5 mm high, pistil ca. 2 cm long; ovary linear, ca. 13 mm long, ca. 1.2 mm in diam. Ovary green,
nearly glabrous; style white, with extremely white pubescent hairs, ca. 7 mm long. Stigmas 2, ovate, 0.3 mm long for
each one. Fruit not seen.
Etymology:The epithet, asterocalyx”, means that the calyx lobes of this new species are actinomorphic and
the whole calyx looks like a star.
Vernacular name:—Xíng È Shí Shân Jǜ Tái (星萼石山苣苔).
Habitat and distribution:—Petrocodon asterocalyx is found growing on slopes and cliffs of some conglomerate
or sandstone hills, often at crevices in evergreen-broadleaf forest. It is only known in Ziyuan County, Guangxi, South
China, where it is of a seasonal monsoon climate. The distribution of this new species and its relatives, P. hancei and
P. coriaceifolius, are shown in Figure 4.
FIGURE 4 Distribution of Petrocodon asterocalyx (A) and its morphologically similar species, P. hancei (B, elliptical area) and relative
P. coriaceifolius (C).
Discussion
Our analyses of molecular data suggest Petrocodon asterocalyx fall into a weakly supported clade. Despite the
systematic placement of the new species is uncertain indicating more DNA regions are needed in further studies to
resolve this problem, we can easily distinguish the new species from others within this clade. The morphological
differences of peduncle, calyx lobes, corolla and stamens of the three related species are presented in the Table 2 & 3
and Figure 5.
PETROCODON ASTEROCALYX Phytotaxa 343 (3) © 2018 Magnolia Press 265
FIGURE 5 The morphological and molecular related species, Petrocodon hancei and P. coriaceifolius. (A–F) P. hancei: (A) habitat, (B)
habit, (C) cymes and frontal view of flowers, (D) cymes and buds, (E) calyx lobes and buds, and (F) stigma; (G–K) P. coriaceifolius: (G)
habitat, (H) habit and cyme, (I) frontal and lateral view of flowers, (J) top view of flowers and buds, and (K) stigma.
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266 Phytotaxa 343 (3) © 2018 Magnolia Press
TABLE 2 Morphological comparison of Petrocodon asterocalyx and P. hancei
P. asterocalyx P. hancei
Peduncle sparsely hispid, with unapparent nodules sparsely puberulent to strigose
Pedicel length 10–20 mm long 5–12 mm long
Calyx lobes linear, 20–40 × 2–3 mm; actinomorphic from the
base linear to narrowly triangular, 2.5–7 × 0.5–1.2
mm; close to the corolla tube
Corolla 2.5–3.0 cm long 1.5–2 cm long
Corolla lips adaxial lip ca. 7 mm long, abaxial lip 18–20 mm
long adaxial lip 3–5 mm long, abaxial lip 4–8.5 mm
long
Filaments 7.8–8.5 mm long, sparsely erectly pubescent 6–7 mm long, glabrous
Anthers 3.5–3.8 mm long, sparsely pubescent 1.5–3 mm long, glabrous
Staminodes 3 2
Stigmas 2, lobes ligulate 1, disciform
TABLE 3 Diagnostic characters of Petrocodon asterocalyx and the similar species P. coriaceifolius
P. asterocalyx P. coriaceifolius
Leaf blades rhombic-oblong or rhombic, base shallowly
cuneate, margin crenulate to serrate ovate-elliptic to ovate-oblong, base
shallowly cordate, margin entire
Pedicel length 10–20 mm long 3–5 mm long
Calyx lobes linear, 20–40 × 2–3 mm, actinomorphic
from the base of the corolla tube lanceolate-linear, 2–4 × 0.2–0.3 mm,
appressed to the corolla tube
Corolla lips adaxial lip ca. 7 mm long, abaxial lip 18–20
mm long adaxial lip ca. 3 mm long, abaxial lip 7–10
mm long
Filaments 7.8–8.5 mm long, sparsely erectly pubescent 5–6 mm long, pubescent
Anthers 3.5–3.8 mm long, sparsely pubescent,
elliptical ca.1.5 mm long, glabrous, reniform
Acknowledgments
We are grateful to Ms. Xuan Wen for the botanical illustration. This study was financially supported by the Fund
of Guangxi Key Laboratory of Plant Conservation and Restoration Ecology in Karst Terrain (16-A-01-01 & 02),
the National Natural Science Foundation of China (Grant No. 31460159), Science Research Foundation of Guangxi
Academy of Sciences (no. 2017YJJ23022), Guangxi Natural Science Foundation Program (2017GXNSFAA19800
6),Scientific Research Foundation of Beijing Municipal Education Commission (KM201410020009), and Opening
Foundation of Beijing Engineering Research Center of Rural Landscape Planning and Design (kf2017061).
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... After consulting the monographs Wang 2005, Wei et al. 2010) and comparing the species with all other congeners described (i.e. Chen et al. 2014, Chen et al. 2019, Xu et al. 2014, Hong et al. 2014, Guo et al. 2016, Cen et al. 2017, Lu et al. 2017b, Zhang et al. 2018, Li et al. 2019) and specimens of Gesneriaceae deposited at IBSC, IBK, KUN, PE, US and VMN. We confirmed that it is a new species and hence we describe and illustrate it below as such. ...
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... The ingroup contained 22 species of Petrocodon, while Primulina dryas (Dunn) Mich. Möller & A. Weber and P. pinnata (W.T.Wang) Y.Z.Wang were chosen as outgroup based on recent phylogenetic analyses (Li and Wang 2007, Möller et al. 2011, Weber et al. 2011, Zhang et al. 2018. All sequences are listed in Table 1. ...
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Based on molecular studies, the small Chinese genus Petrocodon (two species and one variety) has been recently enlarged to include the monotypic genera Calcareoboea, Paralagarosolen and Tengia. It is shown here that the (6-7) species of Lagarosolen, the monotypic Dolicholoma, a few species of Didymocarpus, and a number of new species that have recently been published (but not formally described) under Petrocodon and Lagarosolen should be included in this genus. This raises the size of the genus from five to around 20 species. With respect to the floral diversity (corolla form, size, and coloration; with the exception of Tengia, the androecium is always diandrous) and inferred pollination syndromes (different forms of melittophily, ornithophily, psycho- and/or sphingophily), Petrocodon represents one of the most varied genera of Old World Gesneriaceae, comparable to some New World genera.
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Petrocodon hunanensis, a new species of Gesneriaceae from limestone area in Hunan Province, China, is described and illustrated. The new species is morphologically similar to Petrocodon coriaceifolius, but readily differs from the latter one in the growth form with terrestrial stems and distinct internodes, both surfaces of leaf densely with white pubescence, petiole densely with reddish-purple or white pubescence, zygomorphic corolla white or pale purple and 2-3 cm long, pedicels 0.3-2 (2.8) cm long, 4 stamens and 1 staminodes, ovary and capsule stipitate. Molecular evidences indicate that it is systematically similar to P. hispidus, but the morphologies of two relatives are obviously different.
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Petrocodon pulchriflorus, a new species of Gesneriaceae is described and illustrated from the limestone area of southwestern Guangxi, China. It is similar to Petrocodon guangxiensis, but can be distinguished by several characters, such as the size and shape of leaf blades, inflorescences and corolla, and the position of stamens. Also the phylogenetic affinity of Petrocodon pulchriflorus is presented based on the plastid trnL-F and nuclear ribosomal ITS sequences.
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Paralagarosolen fangianum Y. G. Wei, a new genus and species of Gesneriaceae from Guangxi, China, is described and illustrated. Paralagarosolen Y. G. Wei is closely related to Lagarosolen W. T. Wang in having corolla tube cylindric, not swollen, and stigmas 2, but differs by having leaves sometimes peltate at base, cyme with only one flower, corolla lobes rounded-obtuse at apex, and capsule broadly ovoid-ellipsoid.
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Background – Anna is a small genus of Gesneriaceae, occurring in China and Vietnam. During fieldwork in Guizhou, China, a representative of this genus was discovered which did not match the description of any of the three known species. Methods – Herbarium taxonomical and morphological studies, SEM of pollen and seeds and molecular phylogenetic analyses were performed. Key results – A new species, Anna rubidiflora S.Z. He, F.Wen & Y.G. Wei, a stenochoric species from the southern part of China (Kaiyang county of Guizhou province), is described and illustrated. It differs from the morphologically similar species A. ophiorrhizoides (Hemsl.) Burtt & Davidson by being a larger plant with undivided stem, its entire leaf blade margins, its abaxial greenish-white leaf blades with slightly purple nerves, its obovate-lanceolate calyx lobes with truncate and nearly rounded apex without nerves, and its reddish-purple corolla. The newly described species is illustrated both by a line drawing and photographs. A locality and distribution map is also presented. A phylogenetic analysis confirmed that A. rubidiflora is a member of Anna and a close relative of A. ophiorrhizoides.